| differentiation of species and strains of entomopathogenic fungi by random amplification of polymorphic dna (rapd). | polymerase chain reaction (pcr)-based technology, involving random amplification of polymorphic dna (rapd), was used to assess the genomic variability between 24 isolates of deuteromycetous fungi (metarhizium anisopliae, metarhizium flavoviride, unidentified strains of metarhizium and beauveria bassiana) which were found to infect grasshoppers or locusts. m. flavoviride showed little intraspecific variability in pcr-amplified fragments when compared to m. anisopliae. the high level of variabilit ... | 1994 | 8087878 |
| pathological and physiological responses of ring-necked pheasant chicks following dietary exposure to the fungus metarhizium flavoviride, a biocontrol agent for locusts in africa. | metarhizium flavoviride, a fungal pathogen of grasshoppers and locusts, appears to be an effective, non-chemical insecticide (mycoinsecticide) for control of grasshoppers and locusts. this study, conducted during june and july, 1997, examined the pathogenic potential of this entomopathogenic fungus to non-target avian species that encounter infected insect prey items or contaminated food sources. ring-necked pheasant (phasianus colchicus) chicks were exposed to one of three diets, (spore-coated ... | 1999 | 10231746 |
| pathogenicity of four strains of entomopathogenic fungi against the bovine tick boophilus microplus. | to evaluate the pathogenicity of 4 strains of the entomopathogenic fungi metarhizium flavoviride var flavoviride and metarhizium anisopliae var anisopliae in vitro against the bovine tick boophilus microplus. | 2001 | 11560280 |
| pathogenicity of entomopathogenic fungi to different developmental stages of rhipicephalus sanguineus (acari: ixodidae). | the pathogenicity of 4 species of entomopathogenic fungi (hyphomycetes species: beauveria bassiana, metarhizium anisopliae, metarhizium flavoviride, and paecilomyces fumosoroseus) to various developmental stages of rhipicephalus sanguineus (acari: ixodidae) ticks was compared under laboratory conditions. the most virulent isolate, m. anisopliae-108, caused 92-96% mortality to unfed larvae and nymphs on day 7 postinfection (pi) and 100% mortality to unfed adults and engorged females on day 21 pi. ... | 2001 | 11780821 |
| comparison of non-linear temperature-dependent development rate models applied to in vitro growth of entomopathogenic fungi. | five non-linear models with three to five parameters, built to quantify the effect of temperature on insect development and microbial growth, were tested to describe the influence of temperature on in vitro-measured growth rates of entomopathogenic hyphomycetes. data from two isolates of each of the four fungal species, paecilomyces fumosoroseus, beauveria bassiana, metarhizium anisopliae, metarhizium flavoviride, were used to assess the features of each model. criteria for model evaluation incl ... | 2003 | 15000248 |
| nucleotide sequence variation does not relate to differences in kinetic properties of neutral trehalase from the insect pathogenic fungus metarhizium anisopliae. | genetic variability in a putative virulence factor, the neutral trehalase ( ntl) gene, was examined in strains of the insect pathogenic fungi metarhizium anisopliae and metarhizium flavoviride by restriction fragment length polymorphism (rflp). the ntl gene was sequenced from four of these strains that showed dissimilar rflp patterns. enzyme kinetic experiments were also performed on the partially purified neutral trehalase in order to assess whether nucleotide changes in these strains related t ... | 2004 | 15170238 |
| cultivation of entomopathogenic fungi for the search of antibacterial compounds. | entomopathogenic fungi are a rich source of natural bioactive compounds. to establish cultivation conditions which facilitate the production of bioactive compounds and to select good genera among entomopathogenic fungi as the producer, 47 typical entomopathogenic fungi were tested for their ability to produce antibiotic activity. thirty-eight strains (81%) and 30 strains (64%) of these fungi produced either anti-bacillus compounds or anti-staphylococcus compounds, respectively, indicating that t ... | 2005 | 16244901 |
| characterization and ultrastructural localization of chitinases from metarhizium anisopliae, m. flavoviride, and beauveria bassiana during fungal invasion of host (manduca sexta) cuticle. | extracellular chitinases have been suggested to be virulence factors in fungal entomopathogenicity. we employed isoelectric focusing and a set of three fluorescent substrates to investigate the numbers and types of chitinolytic enzymes produced by the entomopathogenic fungi metarhizium anisopliae, metarhizium flavoviride, and beauveria bassiana. each species produced a variety of n-acetyl-(beta)-d-glucosaminidases and endochitinases during growth in media containing insect cuticle. m. flavovirid ... | 1996 | 16535278 |
| [the influence of the metarhizium fungi on the embryonic development of ascaris suum]. | purpose: the present study focused on the effect of two fungal species metarhizium flavoviride and metarhizium anisopliae on the embryonic development and viability of ascaris suum larvae. results: microscope examination revealed that the presence of the mycelium inhibited the egg development, compared to control. the fungus-exposed eggs featured zygote vacuolisation, irregular blastomere cleavage, and morphological disturbances in the stages of blastula, gastrula, and larva. the embryopathies w ... | 2006 | 17131843 |
| metarhizium frigidum sp. nov.: a cryptic species of m. anisopliae and a member of the m. flavoviride complex. | the anamorph genus metarhizium is composed of arthropod pathogens, several with broad geographic and host ranges. members of the genus, including "m. anisopliae var. frigidum" nomen nudum and metarhizium flavoviride, have been used as biological insecticides. in a recent revision of the genus the variety "m. anisopliae var. frigidum" was suggested to be a synonym of m. flavoviride based largely on its sequence phylogenetic analysis. in this study we conducted morphological evaluations and multig ... | 2006 | 17256577 |
| isolation of entomopathogenic fungi from northern thailand and their production in cereal grains. | spore productivity in six entomopathogenic fungal strains isolated from insect cadavers at four locations in chiang mai province was evaluated in five cereal grains: white-rice, wheat, rye, corn and sorghum. according to sequence analysis of the internal transcribed spacer regions of these isolates, they were closely related to beauveria bassiana (2 isolates), metarhizium flavoviride (1 isolate), metarhizium anisopliae (1 isolate), paecilomyces lilacinus (1 isolate) and isaria tenuipes (1 isolat ... | 2012 | 22965474 |
| a natural fungal infection of a sylvatic cockroach with metarhizium blattodeae sp. nov., a member of the m. flavoviride species complex. | a wild, forest-dwelling cockroach from the subfamily ectobiidae (order blattodea) in a nature reserve in cavalcante, in the state of goiás, brazil, was found to be infected by a new, genetically distinct species in the metarhizium flavoviride species complex that we describe here as metarhizium blattodeae. the status of this fungus as a new species is supported by both multigenic sequence comparisons and protein profiles generated by maldi-tof (matrix-assisted laser desorption/ionization time-of ... | 2016 | 27109363 |
| diversity within the entomopathogenic fungal species metarhizium flavoviride associated with agricultural crops in denmark. | knowledge of the natural occurrence and community structure of entomopathogenic fungi is important to understand their ecological role. species of the genus metarhizium are widespread in soils and have recently been reported to associate with plant roots, but the species m. flavoviride has so far received little attention and intra-specific diversity among isolate collections has never been assessed. in the present study m. flavoviride was found to be abundant among metarhizium spp. isolates obt ... | 2015 | 26519342 |
| metarhizin a suppresses cell proliferation by inhibiting cytochrome c oxidase activity. | metarhizin a was originally isolated from metarhizium flavoviride as a potent inhibitor of the growth of insect and mammalian cells. in this study, we aimed to understand the molecular targets of metarhizin a involved in its anti-proliferative activity against human cells. | 2014 | 24699005 |
| effect of liquid culture media on morphology, growth, propagule production, and pathogenic activity of the hyphomycete, metarhizium flavoviride. | two isolates of metarhizium spp. were studied for propagule production, because of their pathogenic activity towards locusts and grasshoppers (mf189 = m. flavoviride (or m. anisopliae var. acridum) strain imi 330189, and mf324 = m. flavoviride strain arsef324). both isolates were grown in seven different liquid media, which have been developed for mass production of various hyphomycetes, considered as candidates for microbial control of noxious insects. shake-flask experiments were carried out a ... | 2002 | 12171445 |
| cuticle-degrading proteases from the entomopathogen metarhizium flavoviride and their distribution in secreted and intracellular fractions. | the aim of this study was to analyse a native isolate of metarhizium flavoviride (cg423) which is being developed as a myco-insecticide against grasshoppers in brazil for the production of the cuticle-degrading subtilisin-like (pr1), and trypsin-like (pr2) proteases. | 2002 | 11849501 |
| genetic variability in metarhizium flavoviride revealed by telomeric fingerprinting. | previous studies using arbitrarily primed pcr (ap-pcr/rapd) analysis have shown only little genetic variation among isolates of the entomopathogenic fungus metarhizium flavoviride. in the current study, however, telomeric fingerprinting unambiguously differentiated several brazilian strains of m. flavoviride as well as strains from africa and australia. using this technique, similarity estimates of telomeric dna among distinct strains were less than 50%, showing this locus to be highly mutable i ... | 1999 | 10481085 |
| double-stranded rna in the entomopathogenic fungus metarhizium flavoviride. | bands of dsrna were detected in five out of seven isolates of the entomopathogenic fungus metarhizium flavoviride. the identity of these bands was proven by rnase and s1-nuclease treatments. the transference of dsrna between isolates (from cg291 to cg442) was successfully carried out through forced heterokaryons. isogenic strains, with or without dsrna, were submitted to virulence tests against the grasshopper rhammatocerus schistocercoides. in contrast to what has been found in some phytopathog ... | 1999 | 10447600 |
| virulence of the entomopathogenic fungus metarhizium flavoviride gams and rozsypal and toxicity of diflubenzuron, fenitrothion-esfenvalerate and profenofos-cypermethrin to nontarget arthropods in mauritania. | within the framework of the gtz project, biological and integrated control of locusts and grasshoppers, a laboratory screening fo rmetarhizium flavoviride (strain mfl 5) blastospore pathogenicity to the nontarget arthropods pharoscymnus anchorago f. (coleoptera:coccinellidae), trachyderma hispida (forskâl) (coleoptera:tenebrionidae), palpares cf. tesselatus rambur (neuroptera:myrmeleontidae) and thanatus sp. (araneae: philodromidae) was conducted in akjoujt research station, mauritania. various ... | 1997 | 9002437 |