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genomic analysis of oryctes rhinoceros virus reveals genetic relatedness to heliothis zea virus 1.oryctes rhinoceros virus (orv) is an unassigned invertebrate dsdna virus with enveloped and rod-shaped virions. two cloned psti fragments, c and d, of orv dna have been sequenced, consisting of 19,805 and 17,146 bp, respectively, and comprising about 30% of the orv genome. for each of the two fragments, 20 open reading frames (orfs) of 150 nucleotides or greater with no or minimal overlap were predicted. ten of the predicted 40 orfs revealed significant similarities to heliothis zea virus 1 (hzv ...200717106621
the genome of gryllus bimaculatus nudivirus indicates an ancient diversification of baculovirus-related nonoccluded nudiviruses of insects.the gryllus bimaculatus nudivirus (gbnv) infects nymphs and adults of the cricket gryllus bimaculatus (orthoptera: gryllidae). gbnv and other nudiviruses such as heliothis zea nudivirus 1 (hznv-1) and oryctes rhinoceros nudivirus (ornv) were previously called "nonoccluded baculoviruses" as they share some similar structural, genomic, and replication aspects with members of the family baculoviridae. their relationships to each other and to baculoviruses are elucidated by the sequence of the compl ...200717360757
sequencing of the large dsdna genome of oryctes rhinoceros nudivirus using multiple displacement amplification of nanogram amounts of virus dna.the genomic sequence analysis of many large dsdna viruses is hampered by the lack of enough sample materials. here, we report a whole genome amplification of the oryctes rhinoceros nudivirus (ornv) isolate ma07 starting from as few as about 10 ng of purified viral dna by application of phi29 dna polymerase- and exonuclease-resistant random hexamer-based multiple displacement amplification (mda) method. about 60 microg of high molecular weight dna with fragment sizes of up to 25 kbp was amplified ...200818598718
the genome of oryctes rhinoceros nudivirus provides novel insight into the evolution of nuclear arthropod-specific large circular double-stranded dna viruses.the oryctes rhinoceros nudivirus (ornv) is a dsdna virus with enveloped, rod-shaped virions. its genome is 127,615-ábp in size and contains 139 predicted protein-coding open reading frames (orfs). in-depth genome sequence comparisons revealed a varying number of shared gene homologues, not only with other nudiviruses (nvs) and baculoviruses, but also with other arthropod-specific large dsdna viruses, including the so-called monodon baculovirus (mbv), the salivary gland hypertrophy viruses (sghvs ...201121380757
a dna virus of drosophila.little is known about the viruses infecting most species. even in groups as well-studied as drosophila, only a handful of viruses have been well-characterized. a viral metagenomic approach was used to explore viral diversity in 83 wild-caught drosophila innubila, a mushroom feeding member of the quinaria group. a single fly that was injected with, and died from, drosophila c virus (dcv) was added to the sample as a control. two-thirds of reads in the infected sample had dcv as the best blast hit ...201122053195
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