Publications

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lunar synchronization of testicular development and steroidogenesis in rabbitfish.lunar synchronization of testicular development in the golden rabbitfish, siganus guttatus, was assessed by measuring changes in sperm motility and conditions in the seminal plasma, and by in vitro production of steroid hormones in testicular fragments and sperm preparations. the duration and percentage of sperm motility was low 1 week before spawning (the new moon), but increased significantly on the day of spawning (the first lunar quarter). during the first lunar quarter, the osmolality decre ...200111399470
lunar synchronization of in vitro steroidogenesis in ovaries of the golden rabbitfish, siganus guttatus (bloch).to assess the relationship between lunar cycle and steroidogenesis in the ovaries of the golden rabbitfish, siganus guttatus, the intact follicles of oocytes were incubated in vitro with human chorionic gonadotropin (hcg) and seven steroid hormones, 17alpha,20beta-dihydroxy-4-pregnen-3-one (dhp), 17alpha,20beta,21-trihydroxy-4-pregnen-3-one (20beta-s), 17alpha-hydroxyprogesterone (17alpha-ohp), progesterone (p), cortisol, estradiol-17beta (e2) and testosterone, during the two lunar phases, the n ...200211825028
molecular cloning and daily variations of the period gene in a reef fish siganus guttatus.as the first step in understanding the molecular oscillation of the circa rhythms in the golden rabbitfish siganus guttatus--a reef fish with a definite lunar-related rhythmicity--we cloned and sequenced a period gene (rfper). the rfper gene contained an open reading frame that encodes a protein consisting of 1,452 amino acids; this protein is highly homologous to per proteins of vertebrates including zebrafish. phylogenetic analyses indicated that the rfper protein is related to the zebrafish p ...200717393198
isolation and cdna cloning of somatolactin in rabbitfish (siganus guttatus).we report the isolation and cdna cloning of somatolactin (sl) from rabbitfish, siganus guttatus. rabbitfish sl was isolated from an alkaline extract of the pituitary glands by gel filtration chromatography on sephadex g-100 and reversed-phase high-performance liquid chromatography. sl was monitored by immunoblotting with flounder sl antiserum. the preparation (yield: 0.86 mg/g wet tissues) contained two immunoreactive bands of 24 and 28 kda on sds-page. overlapping partial cdna clones correspond ...199910417242
isolation, cdna cloning, and growth promoting activity of rabbitfish (siganus guttatus) growth hormone.we report the isolation, cdna cloning, and growth promoting activity of rabbitfish (siganus guttatus; teleostei; perciformes; siganidae) growth hormone (gh). rabbitfish gh was extracted from pituitary glands under alkaline conditions, fractionated by gel filtration chromatography on sephadex g-100, and purified by high-performance liquid chromatography. the fractions containing gh were identified by immunoblotting with bonito gh antiserum. under nonreducing conditions, the molecular weight of ra ...200010642447
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