| sulfur-oxidizing bacteria in soap lake (washington state), a meromictic, haloalkaline lake with an unprecedented high sulfide content. | culture-dependent and -independent techniques were used to study the diversity of chemolithoautotrophic sulfur-oxidizing bacteria in soap lake (washington state), a meromictic, haloalkaline lake containing an unprecedentedly high sulfide concentration in the anoxic monimolimnion. both approaches revealed the dominance of bacteria belonging to the genus thioalkalimicrobium, which are common inhabitants of soda lakes. a dense population of thioalkalimicrobium (up to 10(7) cells/ml) was found at th ... | 2007 | 17114324 |
| occurrence, phylogeny and evolution of ribulose-1,5-bisphosphate carboxylase/oxygenase genes in obligately chemolithoautotrophic sulfur-oxidizing bacteria of the genera thiomicrospira and thioalkalimicrobium. | the occurrence of the different genes encoding ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco), the key enzyme of the calvin-benson-bassham cycle of autotrophic co(2) fixation, was investigated in the members of the genus thiomicrospira and the relative genus thioalkalimicrobium, all obligately chemolithoautotrophic sulfur-oxidizing gammaproteobacteria. the cbbl gene encoding the 'green-like' form i rubisco large subunit was found in all analysed species, while the cbbm gene encoding f ... | 2006 | 16804189 |
| chemolithotrophic haloalkaliphiles from soda lakes. | this paper summarizes recent data on the occurrence and properties of lithotrophic prokaryotes found in extremely alkaline, saline (soda) lakes. among the chemolithotrophs found in these lakes the obligately autotrophic sulfur-oxidizing bacteria were the dominant, most diverse group, best adapted to haloalkaline conditions. the culturable forms are represented by three new genera, thioalkalimicrobium, thioalkalivibrio and thioalkalispira in the gammaproteobacteria. among them, the genus thioalka ... | 2005 | 16329914 |
| fatty acid, compatible solute and pigment composition of obligately chemolithoautotrophic alkaliphilic sulfur-oxidizing bacteria from soda lakes. | salt adaptation in chemolithotrophic alkaliphilic sulfur-oxidizing strains belonging to genera thioalkalimicrobium and thioalkalivibrio has been studied by determination of salt-dependent changes in fatty acid and compatible solute composition. in both alkaliphilic groups, represented by the low salt-tolerant thioalkalimicrobium aerophilum strain al 3t and the extremely salt-tolerant thioalkalivibrio versutus strain alj 15, unsaturated fatty acids predominate over saturated fatty acids. in strai ... | 2005 | 15668017 |
| [resting forms of gram negative chemolithoautotrophic bacteria thioalkalivibrio versutus and thioalkalimicrobium aerophilum]. | the haloalkaliphilic chemoautotrophic gram-negative bacteria thioalkalivibrio versutus, strain al2, and thioalkalimicrobium aerophilum, strain al3, were shown to possess the capacity to produce resting forms, namely cyst-like refractile cells (crc), whose production was controlled by the level of the d1 extracellular factors, exhibiting the function of anabiosis autoinducers. the conditions were elucidated that promoted the formation of crc in the developmental cycles of the cultures studied, in ... | 2003 | 12901006 |
| [low-molecular-weight autoregulatory factors in bacteria thioalkalivibrio versutus and thioalkalimicrobium aerophilum]. | the haloalkaliphilic, lithoautotrophic, sulfur-oxidizing gram-negative bacteria thioalkalivibrio versutus and thioalkalimicrobium aerophilum were found to possess a special system for the autoregulation of their growth. the system includes the extracellular autoinducers of anabiosis (the d1 factor) and autolysis (the d2 factor). the principal components of the d1 factor are alkylhydroxybenzenes. the principal components of the d2 factor are free unsaturated fatty acids dominated by oleic acid is ... | 2002 | 12138750 |
| thioalkalimicrobium cyclicum sp. nov. and thioalkalivibrio jannaschii sp. nov., novel species of haloalkaliphilic, obligately chemolithoautotrophic sulfur-oxidizing bacteria from hypersaline alkaline mono lake (california). | two strains of haloalkaliphilic, obligately autotrophic, sulfur-oxidizing bacteria were isolated from the oxygen-sulfide interface water layer of stratified alkaline and saline mono lake, california, usa. strain alm 1t was a dominant species in enrichment on moderate-saline, carbonate-buffered medium (0.6 m total na+, ph 10) with thiosulfate as an energy source and nitrate as a nitrogen source. cells of alm 1t are open ring-shaped and are non-motile. it has a high growth rate and activity of thi ... | 2002 | 12054257 |
| thioalkalimicrobium aerophilum gen. nov., sp. nov. and thioalkalimicrobium sibericum sp. nov., and thioalkalivibrio versutus gen. nov., sp. nov., thioalkalivibrio nitratis sp.nov., novel and thioalkalivibrio denitrificancs sp. nov., novel obligately alkaliphilic and obligately chemolithoautotrophic sulfur-oxidizing bacteria from soda lakes. | forty-three strains of obligately chemolithoautotrophic sulfur-oxidizing bacteria were isolated from highly alkaline soda lakes in south-east siberia (russia) and in kenya using a specific enrichment procedure at ph 10. the main difference between the novel isolates and known sulfur bacteria was their potential to grow and oxidize sulfur compounds at ph 10 and higher. the isolates fell into two groups that were substantially different from each other physiologically and genetically. most of the ... | 2001 | 11321103 |
| haloalkaliphilic sulfur-oxidizing bacteria in soda lakes. | the existence of chemolithoautotrophic sulfur-oxidizing bacteria (sob) capable of growth in an extremely alkaline and saline environment has not been recognized until recently. extensive studies of saline, alkaline (soda) lakes located in central asia, africa and north america have now revealed the presence, at relatively high numbers, of a new branch of obligately autotrophic sob in these doubly extreme environments. overall more than 100 strains were isolated in pure culture. all of them have ... | 2004 | 16102598 |
| growth physiology and competitive interaction of obligately chemolithoautotrophic, haloalkaliphilic, sulfur-oxidizing bacteria from soda lakes. | two different groups of haloalkaliphilic, obligately autotrophic, sulfur-oxidizing bacteria belonging to the genera thioalkalimicrobium and thioalkalivibrio have recently been discovered in highly alkaline and saline soda lakes. to understand response to their extreme environment and different occurrence in soda lakes, the growth kinetics and competitive behavior of several representatives have been characterized in detail using batch and ph-controlled continuous cultivation. the bacteria belong ... | 2003 | 12768450 |
| identification of cytochrome c oxidase in the alkaliphilic, obligately chemolithoautotrophic, sulfur-oxidizing bacterium 'thioalcalomicrobium aerophilum' strain al 3. | cytochrome c oxidase from the novel alkaliphilic autotrophic sulfur bacterium 'thioalcalomicrobium aerophilum' strain al 3 was isolated and purified 87-fold. spectroscopic analysis revealed the presence of both c- and b-type hemes as well as copper in a ratio of 3:2:1. the purified enzyme consists of three subunits with apparent molecular masses of 41, 34 and 32 kda. the two small subunits contain covalently bound heme c. with tmpd as a substrate the ph optimum was determined to be ph 8.0. in th ... | 1999 | 10481092 |
| the microbial sulfur cycle at extremely haloalkaline conditions of soda lakes. | soda lakes represent a unique ecosystem with extremely high ph (up to 11) and salinity (up to saturation) due to the presence of high concentrations of sodium carbonate in brines. despite these double extreme conditions, most of the lakes are highly productive and contain a fully functional microbial system. the microbial sulfur cycle is among the most active in soda lakes. one of the explanations for that is high-energy efficiency of dissimilatory conversions of inorganic sulfur compounds, both ... | 2011 | 21747784 |