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bartonella koehlerae sp. nov., isolated from cats.two of the 25 bartonella isolates recovered during a prevalence study of bartonella henselae bacteremia in domestic cats from the greater san francisco bay region were found to differ phenotypically and genotypically from all prior b. henselae isolates. these isolates, c-29 and c-30, which were recovered from the blood of two pet cats belonging to the same household, grew on chocolate agar as pinpoint colonies following 14 days of incubation at 35 degrees c in a candle jar but failed to grow on ...199910074535
molecular phylogeny of the genus bartonella: what is the current knowledge?species of the genus bartonella are involved in an increasing variety of human diseases. in addition to the 14 currently recognized species, several bartonella strains have been recovered from a wide range of wild and domestic mammals in europe and america. such a high diversity of geographic distributions, animal reservoirs, arthropod vectors and pathogenic properties makes clarification of our knowledge about the phylogeny of bartonella species necessary. phylogenetic data have been inferred m ...200111410341
first isolation and detection by immunofluorescence assay of bartonella koehlerae in erythrocytes from a french cat. 200312904442
bartonella koehlerae, a new cat-associated agent of culture-negative human endocarditis.bartonella koehlerae is reported for the first time to be a human pathogen that causes culture-negative endocarditis. it is also shown that this species, isolated twice before from domestic cats, can be recovered as well from a stray cat population in israel. this work follows a recent report of the same case in which the causative agent was misidentified as b. henselae, based on serology and pcr-restriction fragment length polymorphism (rflp) analysis (a. schattner, o. zimhony, b. avidor, and m ...200415297484
characterization of the genome composition of bartonella koehlerae by microarray comparative genomic hybridization profiling.bartonella henselae is present in a wide range of wild and domestic feline hosts and causes cat-scratch disease and bacillary angiomatosis in humans. we have estimated here the gene content of bartonella koehlerae, a novel species isolated from cats that was recently identified as an agent of human endocarditis. the investigation was accomplished by comparative genomic hybridization (cgh) to a microarray constructed from the sequenced 1.93-mb genome of b. henselae. control hybridizations of labe ...200516109957
feline bartonellosis and cat scratch disease.bartonella species are important emerging zoonotic pathogens. transmission of these organisms in nature may be much more complex than is currently appreciated. cats can be infected with five bartonella species, including, bartonella henselae, bartonella clarridgeae, bartonella bovis, bartonella koehlerae and bartonella quintana. in addition to cats, numerous domestic and wild animals, including bovine, canine, human, and rodent species can serve as chronically infected reservoir hosts for variou ...200818295347
molecular detection of bartonella henselae and bartonella koehlerae from aortic valves of boxer dogs with infective endocarditis.cardiac aortic valves from five dogs that died from acquired infective endocarditis were retrospectively molecularly screened for bartonella infection. identification was carried out using pcr targeting four gene fragments (rpob, ribc, 16s rrna and glta), and the 16s-23s intergenic spacer (its). bartonella henselae dna was detected in aortic valve tissue from one boxer dog with moderate subaortic stenosis (sas). bartonella koehlerae dna was detected from the aortic valve of another boxer dog wit ...201019716241
bartonellosis, an increasingly recognized zoonosis.cat scratch disease is the most common zoonotic infection caused by bartonella bacteria. among the many mammals infected with bartonella spp., cats represent a large reservoir for human infection, as they are the main reservoir for bartonella henselae, bartonella clarridgeiae and bartonella koehlerae. bartonella spp. are vector-borne bacteria, and transmission of b. henselae by cat fleas occurs mainly through infected flea faeces, although new potential vectors (ticks and biting flies) have been ...201020148999
pcr amplification of bartonella koehlerae from human blood and enrichment blood cultures.abstract:201020735840
prevalence of rickettsia felis and the first identification of bartonella henselae fizz/cal-1 in cat fleas (siphonaptera: pulicidae) from taiwan.cat fleas (ctenocephalides felis [bouché]) are the primary ectoparasites of dog and cat populations. in this study, we report the monthly population dynamics of rickettsia felis and bartonella spp. (two zoonotic pathogens that can cause human disease) in cat fleas collected from dogs and cats in taipei, taiwan, from december 2006 to december 2007. natural r. felis infection in individual cat fleas was assessed by polymerase chain reaction (pcr) using prf-, ompb-, and glta-specific primer pairs. ...201121485388
molecular and serological diagnosis of bartonella infection in 61 dogs from the united states.background: molecular diagnosis of canine bartonellosis can be extremely challenging and often requires the use of an enrichment culture approach followed by pcr amplification of bacterial dna. hypotheses: (1) the use of enrichment culture with pcr will increase molecular detection of bacteremia and will expand the diversity of bartonella species detected. (2) serological testing for bartonella henselae and bartonella vinsonii subsp. berkhoffii does not correlate with documentation of bacteremia ...201121615498
co-infection with anaplasma platys, bartonella henselae, bartonella koehlerae and 'candidatus mycoplasma haemominutum' in a cat diagnosed with splenic plasmacytosis and multiple myeloma.anaplasma platys (apl), 'candidatus mycoplasma haemominutum' (cmh), bartonella henselae (bh) and bartonella koehlerae (bk) were confirmed by polymerase chain reaction (pcr) amplification and dna sequencing in a cat diagnosed with multiple myeloma. other inconsistently documented hematologic abnormalities included anemia, thrombocytopenia, eosinophilia and hypoglycemia. persistent apl infection was confirmed for the first time in a north american cat by sequencing three bacterial genes (16s rrna, ...201424445821
experimental infection of cats with afipia felis and various bartonella species or subspecies.based upon prior studies, domestic cats have been shown to be the natural reservoir for bartonella henselae, bartonella clarridgeiae and bartonella koehlerae. however, other bartonella species, such as bartonella vinsonii subsp. berkhoffii, bartonella quintana or bartonella bovis (ex weissii) have been either isolated from or bartonella dna sequences pcr amplified and sequenced. in the late 1980s, before b. henselae was confirmed as the etiological agent of cat scratch disease, afipia felis had ...201424972870
an unmatched case controlled study of clinicopathologic abnormalities in dogs with bartonella infection.we compared clinicopathologic findings in dogs with bartonella infection to bartonella spp. negative dogs suspected of a vector-borne disease. cases (n=47) and controls (n=93) were selected on the basis of positive or negative enrichment culture pcr results, respectively. signalment, clinicopathologic findings and treatments were extracted from medical records. dna sequencing identified bartonella henselae (n=28, 59.6%), bartonella vinsonii subsp. berkhoffii (n=20, 42.6%), bartonella koehlerae ( ...201323683861
prevalence, hematological findings and genetic diversity of bartonella spp. in domestic cats from valdivia, southern chile.the present study determined the prevalence, hematological findings and genetic diversity of bartonella spp. in domestic cats from valdivia, southern chile. a complete blood count and nuog gene real-time quantitative pcr (qpcr) for bartonella spp. were performed in 370 blood samples from cats in valdivia, southern chile. nuog qpcr-positive samples were submitted to conventional pcr for the glta gene and sequencing for species differentiation and phylogenetic analysis. alignment of glta gene was ...201627938417
relationship between the presence of bartonella species and bacterial loads in cats and cat fleas (ctenocephalides felis) under natural conditions.cats are considered the main reservoir of three zoonotic bartonella species: bartonella henselae, bartonella clarridgeiae, and bartonella koehlerae. cat fleas (ctenocephalides felis) have been experimentally demonstrated to be a competent vector of b. henselae and have been proposed as the potential vector of the two other bartonella species. previous studies have reported a lack of association between the bartonella species infection status (infected or uninfected) and/or bacteremia levels of c ...201526070666
bartonella species in fleas from palestinian territories: prevalence and genetic diversity.bartonellosis is an infectious bacterial disease. the prevalence and genetic characteristics of bartonella spp. in fleas of wild and domestic animals from palestinian territories are described. flea samples (n=289) were collected from 121 cats, 135 dogs, 26 hyraxes and seven rats from northern (n=165), central (n=113), and southern palestinian territories (n=11). the prevalent flea species were: ctenocephalides felis (n=119/289; 41.2%), ctenocephalides canis (n=159/289; 55%), and xenopsylla sp. ...201425424254
bartonellae in domestic and stray cats from israel: comparison of bacterial cultures and high-resolution melt real-time pcr as diagnostic methods.to determine the occurrence of feline bartonellosis in israel, blood samples were collected from 179 stray and 155 domestic cats from 18 cities or villages in central and northcentral israel. samples were screened for bartonella infection by culture isolation and molecular detection using high-resolution melt (hrm) real-time pcr assay targeting the 16s-23s rrna internal transcribed spacer (its). all positive samples were confirmed by two additional hrm real-time pcr assays targeting two fragment ...201324107217
ctenocephalides felis an in vitro potential vector for five bartonella species.the blood-sucking arthropod ctenocephalides felis has been confirmed as a vector for bartonella henselae and is a suspected vector for bartonella clarridgeiae, bartonella quintana and bartonella koehlerae in bartonella transmission to mammals. to understand the absence of other bartonella species in the cat flea, we have developed an artificial flea-feeding method with blood infected successively with five different bartonella species. the results demonstrated the ability of these five bartonell ...201323200028
prevalence of bartonella species, hemoplasmas, and rickettsia felis dna in blood and fleas of cats in bangkok, thailand.flea infestations are common in thailand, but little is known about the flea-borne infections. fifty flea pools and 153 blood samples were collected from client-owned cats between june and august 2009 from veterinary hospitals in bangkok, thailand. total dna was extracted from all samples, and then assessed by conventional pcr assays. the prevalence rates of bartonella spp. in blood and flea samples were 17% and 32%, respectively, with dna of bartonella henselae and bartonella clarridgeiae being ...201222521739
isolation of bartonella henselae, bartonella koehlerae subsp. koehlerae, bartonella koehlerae subsp. bothieri and a new subspecies of b. koehlerae from free-ranging lions (panthera leo) from south africa, cheetahs (acinonyx jubatus) from namibia and captive cheetahs from california.bartonellae are blood- and vector-borne gram-negative bacteria, recognized as emerging pathogens. whole-blood samples were collected from 58 free-ranging lions (panthera leo) in south africa and 17 cheetahs (acinonyx jubatus) from namibia. blood samples were also collected from 11 cheetahs (more than once for some of them) at the san diego wildlife safari park. bacteria were isolated from the blood of three (5%) lions, one (6%) namibian cheetah and eight (73%) cheetahs from california. the lion ...201627453220
isolation of bartonella henselae and two new bartonella subspecies, bartonellakoehlerae subspecies boulouisii subsp. nov. and bartonella koehlerae subspecies bothieri subsp. nov. from free-ranging californian mountain lions and bobcats.domestic cats are the natural reservoir of bartonella henselae, b. clarridgeiae and b. koehlerae. to determine the role of wild felids in the epidemiology of bartonella infections, blood was collected from 14 free-ranging california mountain lions (puma concolor) and 19 bobcats (lynx rufus). bartonella spp. were isolated from four (29%) mountain lions and seven (37%) bobcats. these isolates were characterized using growth characteristics, biochemical reactions, molecular techniques, including pc ...201626981874
bartonella species identified in rodent and feline hosts from island and mainland western australia.bacteria of the genus bartonella have been described in multiple mammalian hosts with many species capable of causing disease in humans. cats and various species of rats have been reported to play a role as vertebrate hosts to a number of bartonella spp. this study aimed to identify bartonella spp. in western australia, dirk hartog island (dhi), and christmas island (ci) and to investigate the presence of potential arthropod vectors. feral cats were collected from ci (n = 35), dhi (n = 23) and s ...201626900749
bartonella henselae and b. koehlerae dna in birds. 201424589235
analysis of seroreactivity against cell culture-derived bartonella spp. antigens in dogs.little is known about the specificity of bartonella spp. immunofluorescent antibody (ifa) assays in dogs. bacteremia in sick dogs most often has been associated with bartonella henselae (bh), bartonella vinsonii subspecies berkhoffii (bvb), and bartonella koehlerae (bk). clarification of the diagnostic utility of ifa serology when testing against these organisms is needed.201724341682
experimental infection of dogs with bartonella henselae and bartonella vinsonii subsp. berkhoffii.the lack of a suitable infection model remains an important obstacle for the pathophysiological understanding of bartonella spp. the following pilot study was designed to determine whether cell culture-grown bartonella henselae sa2 and bartonella vinsonii subsp. berkhoffii genotype iii would cause persistent bacteremia in dogs. pre-inoculation screening established that two laboratory-raised golden retrievers were naturally-infected with bartonella koehlerae. despite prior infection, one dog eac ...201324120155
spontaneous onset of complex regional pain syndrome type i in a woman infected with bartonella koehlerae.after a short-term fever, complex regional pain syndrome, characterized by hyperalgesia, intermittent swelling, erythema and cyanosis of both feet, was diagnosed in a female veterinarian. the woman was infected with bartonella koehlerae and she was also bartonella vinsonii subsp. berkhoffii seroreactive. having failed other treatments, symptoms resolved following initiation of antibiotics.201424323298
hallucinations, sensory neuropathy, and peripheral visual deficits in a young woman infected with bartonella koehlerae.a young woman experiencing depression, anxiety, mood swings, severe headaches, muscle spasms, interphalangeal joint stiffness, decreased peripheral vision, diminished tactile sensation, and hallucinations was persistently bartonella koehlerae seroreactive and bacteremic. following antibiotic treatment, b. koehlerae antibodies and dna were not detected and all symptoms resolved.201121734026
experimental infection of domestic cats with bartonella koehlerae and comparison of protein and dna profiles with those of other bartonella species infecting felines.bartonella koehlerae, a recently described feline bartonella species, was isolated from two naturally infected cats in northern california. we experimentally infected domestic cats with b. koehlerae to establish the microbiological and immunological characteristics of this infection in cats and to compare it to infections with those caused by b. henselae and b. clarridgeiae. four cats were inoculated intradermally with b. koehlerae (8.6 x 10(7) to 3.84 x 10(8) cfu/ml). none of the cats presented ...200211825958
first description of bartonella koehlerae infection in a spanish dog with infective endocarditis.bartonella koehlerae has been recently described as a new cat- and cat fleas-associated agent of culture-negative human endocarditis. it has been also encountered in one dog from israel and six dogs from the usa, but other clinically relevant reports involving this bacterium are lacking.201728526098
molecular detection of vector-borne pathogens in greek cats.infectious diseases have been increasingly recognized in cats worldwide. the objective of this study was the molecular investigation of the prevalence of selected pathogens in healthy and sick cats from greece, a country highly endemic for several canine vector-borne pathogens. blood and/or bone marrow samples from 50 clinically healthy and 50 sick adult (>1 year-old) cats were retrospectively examined for the amplification of bartonella spp., haemoplasmas, ehrlichia spp., anaplasma spp., babesi ...201728887102
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