| isolation and molecular characterization of a novel cytopathogenic paramyxovirus from tree shrews. | a cytopathic infectious agent was isolated from the kidneys of an apparently healthy tree shrew (tupaia belangeri) that had been captured in the area around bangkok. the infectivity was propagated in tupaia fibroblast and kidney cell cultures. paramyxovirus-like pleomorphic enveloped particles and helical nucleocapsids were observed by electron microscopy and accordingly the infectious agent was termed tupaia paramyxovirus (tpmv). however, no serological cross-reactions were detected between tpm ... | 1999 | 10366580 |
| identification and phylogenetic comparison of salem virus, a novel paramyxovirus of horses. | a virus that could not be identified as a previously known equine virus was isolated from the mononuclear cells of a horse. electron microscopy revealed enveloped virions with nucleocapsid structures characteristic of viruses in the paramyxoviridae family. the virus failed to hemabsorb chicken or guinea pig red blood cells and lacked neuraminidase activity. two viral genes were isolated from a cdna expression library. multiple sequence alignments of one gene indicated an average identity of 45% ... | 2000 | 10793001 |
| full-length genome sequence of mossman virus, a novel paramyxovirus isolated from rodents in australia. | mossman virus (mov) was isolated on two occasions from wild rats trapped in queensland, australia, during the early 1970s. together with nariva virus and j-virus mov belongs to a group of novel paramyxoviruses isolated from rodents during the last 40 years, none of which had been characterized at the molecular level until now. cdna subtraction strategies used to isolate virus-specific cdna derived from both mov-infected cells and crude mov pellets were pivotal steps in rapid characterization of ... | 2003 | 14698671 |
| a tupaia paramyxovirus vector system for targeting and transgene expression. | viruses from the diverse family of paramyxoviridae include important pathogens and are applied in gene therapy and for cancer treatment. the tupaia paramyxovirus (tpmv), isolated from the kidney of a tree shrew, does not infect human cells and neutralizing antibodies against other paramyxoviridae do not cross-react with tpmv. here, we present a vector system for de novo generation of infectious tpmv that allows for insertion of additional genes as well as targeting using antibody single-chain va ... | 2017 | 28809150 |