| asaia bogorensis gen. nov., sp. nov., an unusual acetic acid bacterium in the alpha-proteobacteria. | eight gram-negative, aerobic, rod-shaped and peritrichously flagellated strains were isolated from flowers of the orchid tree (bauhinia purpurea) and of plumbago (plumbago auriculata), and from fermented glutinous rice, all collected in indonesia. the enrichment culture approach for acetic acid bacteria was employed, involving use of sorbitol medium at ph 3.5. all isolates grew well at ph 3.0 and 30 degrees c. they did not oxidize ethanol to acetic acid except for one strain that oxidized ethano ... | 2000 | 10758893 |
| asaia sp., an unusual spoilage organism of fruit-flavored bottled water. | a gram-negative bacillus was isolated from a batch of fruit-flavored bottled water, which had spoiled as a result of bacterial overgrowth (>10(6) cfu/ml). the spoilage organism was extremely difficult to identify phenotypically and was poorly identified as pasturella sp. (78.7% identification profile) employing the api 20ne identification scheme, which gave the profile 5040000. molecular identification through pcr amplification of a partial region of the 16s rrna gene followed by direct automate ... | 2002 | 12147519 |
| lactic acid bacteria associated with wine grapes from several australian vineyards. | the detection and isolation of lactic acid bacteria by enrichment methods from wine grapes cultivated in vineyards located in new south wales, australia. | 2006 | 16553726 |
| first report of bacteremia by asaia bogorensis, in a patient with a history of intravenous-drug abuse. | we report the first documented case of bacteremia caused by asaia bogorensis in a young patient with a history of intravenous-drug abuse. a. bogorensis was identified by sequencing the 16s rrna gene. the isolate was exceptionally resistant to almost all antibiotics that are routinely tested for gram-negative rods but was susceptible to netilmicin, gentamicin, and doxycycline. | 2006 | 16891542 |
| phylogenetic relationships between the genera swaminathania and asaia, with reference to the genera kozakia and neoasaia, based on 16s rdna, 16s-23s rdna its, and 23s rdna sequences. | | 2006 | 17310072 |
| bacteria of the genus asaia stably associate with anopheles stephensi, an asian malarial mosquito vector. | here, we show that an alpha-proteobacterium of the genus asaia is stably associated with larvae and adults of anopheles stephensi, an important mosquito vector of plasmodium vivax, a main malaria agent in asia. asaia bacteria dominate mosquito-associated microbiota, as shown by 16s rrna gene abundance, quantitative pcr, transmission electron microscopy and in situ-hybridization of 16s rrna genes. in adult mosquitoes, asaia sp. is present in high population density in the female gut and in the ma ... | 2007 | 17502606 |
| comparison of two bacteremic asaia bogorensis isolates from europe. | | 2007 | 17562089 |
| asaia, a versatile acetic acid bacterial symbiont, capable of cross-colonizing insects of phylogenetically distant genera and orders. | bacterial symbionts of insects have been proposed for blocking transmission of vector-borne pathogens. however, in many vector models the ecology of symbionts and their capability of cross-colonizing different hosts, an important feature in the symbiotic control approach, is poorly known. here we show that the acetic acid bacterium asaia, previously found in the malaria mosquito vector anopheles stephensi, is also present in, and capable of cross-colonizing other sugar-feeding insects of phyloge ... | 2009 | 19735280 |
| bacterial symbionts of the brown planthopper, nilaparvata lugens (homoptera: delphacidae). | the brown planthopper (nilaparvata lugens stål), the most destructive pest of rice, has been identified, including biotypes with high virulence towards previously resistant rice varieties. there have also been many reports of a yeast-like symbiont of n. lugens, but little is known about the bacterial microbes. in this study, we examined the bacterial microbes in n. lugens and identified a total of 18 operational taxonomic units (otus) representing four phyla (proteobacteria, firmicutes, actinoba ... | 2010 | 20097822 |
| culture-dependent and culture-independent characterization of microorganisms associated with aedes aegypti (diptera: culicidae) (l.) and dynamics of bacterial colonization in the midgut. | in this work we show that the lumen of aedes aegypti midgut is highly colonized by bacteria that were identified by culture-dependent and culture-independent methods. rdna sequences obtained were compared with those from genbank and the main bacterial genera identified were: serratia, klebsiella, asaia, bacillus, enterococcus, enterobacter,kluyvera and pantoea. all genera were identified in midgut except enterobacter that was observed only in eggs. asaia and pantoea were also identified in eggs ... | 2010 | 20434424 |
| robustness of the bacterial community in the cabbage white butterfly larval midgut. | microbial communities typically vary in composition and structure over space and time. little is known about the inherent characteristics of communities that govern various drivers of these changes, such as random variation, changes in response to perturbation, or susceptibility to invasion. in this study, we use 16s ribosomal rna gene sequences to describe variation among bacterial communities in the midguts of cabbage white butterfly (pieris rapae) larvae and examine the influence of community ... | 2010 | 19924467 |
| ameyamaea chiangmaiensis gen. nov., sp. nov., an acetic acid bacterium in the alpha-proteobacteria. | two isolates, ac04(t) and ac05, were isolated from the flowers of red ginger collected in chiang mai, thailand. in phylogenetic trees based on 16s rrna gene sequences, the two isolates were included within a lineage comprised of the genera acidomonas, gluconacetobacter, asaia, kozakia, swaminathania, neoasaia, granulibacter, and tanticharoenia, and they formed an independent cluster along with the type strain of tanticharoenia sakaeratensis. the calculated pair-wise sequence similarities of isol ... | 2009 | 19809199 |
| molecular evidence for multiple infections as revealed by typing of asaia bacterial symbionts of four mosquito species. | the recent increased detection of acetic acid bacteria (aab) of the genus asaia as symbionts of mosquitoes, such as anopheles spp. and aedes spp., prompted us to investigate the diversity of these symbionts and their relationships in different mosquito species and populations. following cultivation-dependent and -independent techniques, we investigated the microbiota associated with four mosquito species, anopheles stephensi, anopheles gambiae, aedes aegypti, and aedes albopictus, which are impo ... | 2010 | 20851960 |
| acetic acid bacteria, newly emerging symbionts of insects. | recent research in microbe-insect symbiosis has shown that acetic acid bacteria (aab) establish symbiotic relationships with several insects of the orders diptera, hymenoptera, hemiptera, and homoptera, all relying on sugar-based diets, such as nectars, fruit sugars, or phloem sap. to date, the fruit flies drosophila melanogaster and bactrocera oleae, mosquitoes of the genera anopheles and aedes, the honey bee apis mellifera, the leafhopper scaphoideus titanus, and the mealybug saccharicoccus sa ... | 2010 | 20851977 |
| asaia lannaensis bloodstream infection in a child with cancer and bone marrow transplantation. | | 2009 | 19502367 |
| arbovirus detection in insect vectors by rapid, high-throughput pyrosequencing. | despite the global threat caused by arthropod-borne viruses, there is not an efficient method for screening vector populations to detect novel viral sequences. current viral detection and surveillance methods based on culture can be costly and time consuming and are predicated on prior knowledge of the etiologic agent, as they rely on specific oligonucleotide primers or antibodies. therefore, these techniques may be unsuitable for situations when the causative agent of an outbreak is unknown. | 2010 | 21085471 |
| bacterial diversity of field-caught mosquitoes, aedes albopictus and aedes aegypti, from different geographic regions of madagascar. | symbiotic bacteria are known to play important roles in the biology of insects, but the current knowledge of bacterial communities associated with mosquitoes is very limited and consequently their contribution to host behaviors is mostly unknown. in this study, we explored the composition and diversity of mosquito-associated bacteria in relation with mosquitoes' habitats. wild aedes albopictus and aedes aegypti were collected in three different geographic regions of madagascar. culturing methods ... | 2010 | 21175696 |
| identification of acetobacter, gluconobacter, and asaia strains isolated in thailand based on 16s-23s rrna gene internal transcribed spacer restriction and 16s rrna gene sequence analyses. | twenty-six strains of acetic acid bacteria were isolated from fruits, flowers and related materials collected in thailand. they were divided into three genera, acetobacter, gluconobacter and asaia, by phenotypic characterization and 16s rrna gene sequence analyses. on the basis of 16s-23s rrna gene internal transcribed spacer (16s-23s rdna its) restriction and 16s rrna gene sequence analyses, fourteen isolates assigned to the genus acetobacter were divided into five groups: 1) group 1a or a. tro ... | 2009 | 21566366 |
| bacteria of the genus asaia: a potential paratransgenic weapon against malaria. | symbiotic bacteria have been proposed as tools for control of insect-borne diseases. primary requirements for such symbionts are dominance, prevalence and stability within the insect body. most of the bacterial symbionts described to date in anopheles mosquitoes, the vector of malaria in humans, have lacked these features. we describe an alpha-proteobacterium of the genus asaia, which stably associates with several anopheles species and dominates within the body of an. stephensi. asaia exhibits ... | 2008 | 18510013 |
| energy metabolism of a unique acetic acid bacterium, asaia bogorensis, that lacks ethanol oxidation activity. | acetic acid bacteria (aab) are known as a vinegar producer on account of their ability to accumulate a high concentration of acetic acid due to oxidative fermentation linking the ethanol oxidation respiratory chain. reactions in oxidative fermentation cause poor growth because a large amount of the carbon source is oxidized incompletely and the harmful oxidized products are accumulated almost stoichiometrically in the culture medium during growth, but a newly identified aab, asaia, has shown unu ... | 2008 | 18391448 |
| tanticharoenia sakaeratensis gen. nov., sp. nov., a new osmotolerant acetic acid bacterium in the alpha-proteobacteria. | tanticharoenia sakaeratensis gen. nov., sp. nov. is proposed for three strains isolated from soil collected in thailand. the three strains, ac37(t), ac38, and ac39, were included within a lineage comprising the genera asaia, kozakia, swaminathania, neoasaia, acetobacter, gluconobacter, and saccharibacter in a phylogenetic tree based on 16s rrna gene sequences, but formed a quite different, independent cluster. pair-wise sequence similarities of strain ac37(t) were 96.5-92.1% to the type strains ... | 2008 | 18323667 |
| asaia lannaensis sp. nov., a new acetic acid bacterium in the alphaproteobacteria. | asaia lannaensis sp. nov. was described for two strains isolated from flowers of the spider lily collected in chiang mai, thailand. the isolates produced acetic acid from ethanol on ethanol/calcium carbonate agar, differing from the type strains of asaia bogorensis, asaia siamensis, and asaia krungthepensis, but did not grow in the presence of 0.35% acetic acid (v/v). the new species is the fourth of the genus asaia, the family acetobacteraceae. | 2008 | 18323663 |
| genera and species in acetic acid bacteria. | taxonomic studies of acetic acid bacteria were historically surveyed. the genus acetobacter was first introduced in 1898 with a single species, acetobacter aceti. the genus gluconobacter was proposed in 1935 for strains with intense oxidation of glucose to gluconic acid rather than oxidation of ethanol to acetic acid and no oxidation of acetate. the genus "acetomonas" was described in 1954 for strains with polar flagellation and no oxidation of acetate. the proposals of the two generic names wer ... | 2008 | 18199517 |
| recent advances in nitrogen-fixing acetic acid bacteria. | nitrogen is an essential plant nutrient, widely applied as n-fertilizer to improve yield of agriculturally important crops. an interesting alternative to avoid or reduce the use of n-fertilizers could be the exploitation of plant growth-promoting bacteria (pgpb), capable of enhancing growth and yield of many plant species, several of agronomic and ecological significance. pgpb belong to diverse genera, including azospirillum, azotobacter, herbaspirillum, bacillus, burkholderia, pseudomonas, rhiz ... | 2008 | 18177965 |
| genus-specific profile of acetic acid bacteria by 16s rdna pcr-dgge. | an effective method for grouping acetic acid bacteria (aab) genera was defined and evaluated as a tool for preliminary screening of the major aab species involved in vinegar production. acetobacter, gluconobacter, gluconacetobacter, asaia, neoasaia, saccharibacter, frateuria and kozakia aab strains were screened on the basis of the 16s rdna sequences using polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge) technique. the dgge profile of all the strains tested, consisted ... | 2008 | 17919758 |
| identification of thai isolates assigned to the genus asaia based on 16s rdna restriction analysis. | | 2007 | 17878666 |
| identification of strains assigned to the genus asaia yamada et al. 2000 based on 16s rdna restriction analysis. | | 2006 | 17116973 |
| identification of strains assigned to the genus asaia yamada et al. 2000 based on restriction analysis of 16s-23s rdna internal transcribed spacer regions. | | 2006 | 16598159 |
| neoasaia chiangmaiensis gen. nov., sp. nov., a novel osmotolerant acetic acid bacterium in the alpha-proteobacteria. | an acetic acid bacterium, designated as isolate ac28(t), was isolated from a flower of red ginger (khing daeng in thai; alpinia purpurata) collected in chiang mai, thailand, at ph 3.5 by use of a glucose/ethanol/acetic acid (0.3%, w/v) medium. a phylogenetic tree based on 16s rrna gene sequences for 1,376 bases showed that isolate ac28(t) constituted a cluster along with the type strain of kozakia baliensis. however, the isolate formed an independent cluster in a phylogenetic tree based on 16s-2 ... | 2005 | 16314684 |
| quick identification of acetic acid bacteria based on nucleotide sequences of the 16s-23s rdna internal transcribed spacer region and of the pqq-dependent alcohol dehydrogenase gene. | acetic acid bacteria (aab) are well known for oxidizing different ethanol-containing substrates into various types of vinegar. they are also used for production of some biotechnologically important products, such as sorbose and gluconic acids. however, their presence is not always appreciated since certain species also spoil wine, juice, beer and fruits. to be able to follow aab in all these processes, the species involved must be identified accurately and quickly. because of inaccuracy and very ... | 2005 | 16261863 |
| swaminathania salitolerans gen. nov., sp. nov., a salt-tolerant, nitrogen-fixing and phosphate-solubilizing bacterium from wild rice (porteresia coarctata tateoka). | a novel species, swaminathania salitolerans gen. nov., sp. nov., was isolated from the rhizosphere, roots and stems of salt-tolerant, mangrove-associated wild rice (porteresia coarctata tateoka) using nitrogen-free, semi-solid lgi medium at ph 5.5. strains were gram-negative, rod-shaped and motile with peritrichous flagella. the strains grew well in the presence of 0.35% acetic acid, 3% nacl and 1% kno3, and produced acid from l-arabinose, d-glucose, glycerol, ethanol, d-mannose, d-galactose and ... | 2004 | 15280289 |
| asaia bogorensis peritonitis identified by 16s ribosomal rna sequence analysis in a patient receiving peritoneal dialysis. | here the authors report a case of refractory peritonitis leading to multiple hospitalizations and the loss of peritoneal dialysis access in a patient on automated peritoneal dialysis, caused by asaia bogorensis, a bacterium not previously described as a human pathogen. this organism was identified by sequence analysis of the 16s ribosomal rna gene. unusual microbial agents may cause peritonitis, and molecular microbiological techniques are important tools for identifying these agents. | 2004 | 15264206 |
| emendation of the genus acidomonas urakami, tamaoka, suzuki and komagata 1989. | the genus acidomonas and the species acidomonas methanolica were recharacterized by using the type strain (nric 0498(t)), three reference strains and 10 methanol-utilizing bacteria that were isolated from activated sludge from three different sewage-treatment plants in tokyo. based on 16s rdna sequences, all strains formed a single cluster within the acetobacteraceae that was clearly different from the genera acetobacter, gluconobacter, gluconacetobacter, asaia and kozakia: the 14 strains were i ... | 2004 | 15143037 |
| asaia krungthepensis sp. nov., an acetic acid bacterium in the alpha-proteobacteria. | three bacterial strains were isolated from flowers collected in bangkok, thailand, by an enrichment-culture approach for acetic acid bacteria. phylogenetic analysis based on 16s rrna gene sequences showed that the isolates were located in the lineage of the genus asaia but constituted a cluster separate from the type strains of asaia bogorensis and asaia siamensis. the dna base composition of the isolates was 60.2-60.5 mol% g+c, with a range of 0.3 mol%. the isolates constituted a taxon separate ... | 2004 | 15023938 |
| kozakia baliensis gen. nov., sp. nov., a novel acetic acid bacterium in the alpha-proteobacteria. | four bacterial strains were isolated from palm brown sugar and ragi collected in bali and yogyakarta, indonesia, by an enrichment culture approach for acetic acid bacteria. phylogenetic analysis based on 16s rrna gene sequences showed that the four isolates constituted a cluster separate from the genera acetobacter, gluconobacter, acidomonas, gluconacetobacter and asaia with a high bootstrap value in a phylogenetic tree. the isolates had high values of dna-dna similarity (78-100%) between one an ... | 2002 | 12054243 |
| asaia siamensis sp. nov., an acetic acid bacterium in the alpha-proteobacteria. | five bacterial strains were isolated from tropical flowers collected in thailand and indonesia by the enrichment culture approach for acetic acid bacteria. phylogenetic analysis based on 16s rrna gene sequences showed that the isolates were located within the cluster of the genus asaia. the isolates constituted a group separate from asaia bogorensis on the basis of dna relatedness values. their dna g+c contents were 58.6-59.7 mol%, with a range of 1.1 mol%, which were slightly lower than that of ... | 2001 | 11321102 |
| mosquito-bacteria symbiosis: the case of anopheles gambiae and asaia. | the symbiotic relationship between asaia, an α-proteobacterium belonging to the family acetobacteriaceae, and mosquitoes has been studied mainly in the asian malaria vector anopheles stephensi. thus, we have investigated the nature of the association between asaia and the major afro-tropical malaria vector anopheles gambiae. we have isolated asaia from different wild and laboratory reared colonies of a. gambiae, and it was detected by pcr in all the developmental stages of the mosquito and in al ... | 2010 | 20571792 |
| asaia spathodeae sp. nov., an acetic acid bacterium in the alpha-proteobacteria. | | 2010 | 20339225 |
| nosocomial infection with asaia lannensis in two paediatric patients with idiopathic dilated cardiomyopathy. | this is the first report, to our knowledge, of two temporally and geographically related nosocomial asaia lannensis infections in a paediatric setting. two patients with idiopathic dilated cardiomyopathy awaiting cardiac transplantation developed bacteraemia during their hospital stay. the physical location of both patients, the temporal association of infections, as well as the isolation of two identical pathogens suggested a nosocomial transmission. commonly used identification methods and ins ... | 2010 | 20339020 |
| bacterial biodiversity and dynamics during malolactic fermentation of tempranillo wines as determined by a culture-independent method (pcr-dgge). | the bacterial population during malolactic fermentation of tempranillo wine was studied using the polymerase chain reaction-denaturing gradient gel electrophoresis, a culture-independent method successfully used for identification and monitoring of bacterial population in different habitats included food fermentations. the results showed that oenococcus oeni was the predominant species in the malolactic fermentation of tempranillo wines, although the presence of gluconobacter oxydans, asaia siam ... | 2010 | 20217079 |
| gluconobacter as well as asaia species, newly emerging opportunistic human pathogens among acetic acid bacteria. | acetic acid bacteria (aab) are broadly used in industrial food processing. among them, members of the genera asaia, acetobacter, and granulibacter were recently reported to be human opportunistic pathogens. we isolated aab from clinical samples from three patients and describe here the clinical and bacteriological features of these cases. we report for the first time (i) the isolation of a gluconobacter sp. from human clinical samples; (ii) the successive isolation of different aab, i.e., an asa ... | 2010 | 20826638 |
| asaia astilbes sp. nov., asaia platycodi sp. nov., and asaia prunellae sp. nov., novel acetic acid bacteria isolated from flowers in japan. | | 2010 | 20953098 |
| ultrafine cellulose fibers produced by asaia bogorensis, an acetic acid bacterium. | the ability to synthesize cellulose by asaia bogorensis, a member of the acetic acid bacteria, was studied in two substrains, aj and jcm. although both strains have identical 16s rdna sequence, only the aj strain formed a solid pellicle at the air-liquid interface in static culture medium, and we analyzed this pellicle using a variety of techniques. in the presence of cellulase, glucose and cellobiose were released from the pellicle suggesting that it is made of cellulose. field emission electro ... | 2011 | 21650167 |
| nitrogen fixation in asaia sp. (family acetobacteraceae). | the genus asaia (family acetobacteraceae) was first introduced with a single species-asaia bogorensis and later six more species were described namely a. siamensis, a. krungthepensis, a. lannaensis, a. platycodi, a. prunellae, and a. astilbes. acetobacteraceae family has been divided into ten genera but, only three of them include nitrogen fixing species: gluconacetobacter, acetobacter, and swaminathania. this article originated from our study primarily aimed to isolate new endosymbiotic nitroge ... | 2011 | 21681635 |
| do mosquito-associated bacteria of the genus asaia circulate in humans? | symbiotic bacteria of the genus asaia have been proposed as tools for control of mosquito-borne diseases, specifically malaria. however, safety issues are a major concern for paratransgenesis strategies. the aim of this study is to investigate, with immunofluorescence assays and quantitative pcr experiments, whether asaia spp. is circulating among humans. all human sera and whole blood samples analyzed were negative for asaia spp., thus suggesting that this organism could be utilized, in the fut ... | 2011 | 21971818 |