| nucleotide sequence of cucurbit aphid-borne yellows luteovirus. | the nucleotide sequence (5669 residues) of the genomic rna of cucurbit aphid-borne yellows luteovirus (cabyv) is presented. analysis of genome organization and sequence homologies indicate that cabyv is a member of luteovirus subgroup 2 (other sequenced members: beet western yellows virus, potato leafroll virus, and barley yellow dwarf virus, rpv isolate) and appears to be most closely related to beet western yellows virus. | 1994 | 8030201 |
| synthesis of a full-length infectious cdna clone of cucurbit aphid-borne yellows virus and its use in gene exchange experiments with structural proteins from other luteoviruses. | a full-length cdna of cucurbit aphid-borne yellows virus (cabyv) has been constructed and expressed either as an in vitro transcript, under control of a bacteriophage t7 rna polymerase promoter, or in vivo, under control of the cauliflower mosaic virus 35s promoter in an agroinfection vector. the biological activity of the cloned cdna was demonstrated by the ability of its in vitro transcript to replicate in protoplasts and of the agroinfection vector to infect agroinoculated plants. virus in th ... | 1995 | 8525610 |
| sequence comparison and classification of beet luteovirus isolates. | three distinct sequence groups were found among partial nucleotide sequences of 38 isolates of beet western yellows virus (bwyv) and beet mild yellowing virus (bmyv) from europe, iran and the usa. the first group contains both sugar beet and oilseed rape specific isolates, and the differentiating characteristic linked to this host range specificity are 2 single base pair changes in a 1,200 nucleotide region of the genome. it is proposed that the european bwyv strains that can be transferred at l ... | 1995 | 8572940 |
| purification and coat protein gene sequence of a montana rmv-like isolate of barley yellow dwarf virus. | a montana barley yellow dwarf virus (bydv) isolate, bydv-rmv-mt, is serologically identical to the new york rmv type isolate (rmv-ny) but differs in aphid transmission phenotype. a purification procedure for bydv-rmv-mt was developed and cdnas encompassing the entire coat protein gene and a portion of the putative polymerase gene of both rmv-mt and rmv-ny were cloned and sequenced. diameters of rmv-mt virions averaged 24.7 nm. average virus yield was 4.2 mg/kg plant tissue. there was 81% sequenc ... | 1996 | 8645094 |
| the genome-linked protein of potato leafroll virus is located downstream of the putative protease domain of the orf1 product. | the sequence of the 32 n-terminal amino acids of the protein (vpg) which is covalently linked to the rna of potato leafroll virus has been determined. the obtained vpg sequence mapped to position 400 to 431 of the plrv orf1 product, downstream of the putative protease domain and in front of the rna-dependent rna polymerase. comparison with other viral sequences revealed significant similarities with the orf1 products of beet western yellows virus, cucurbit aphid-borne yellows virus, and beet mil ... | 1997 | 9268161 |
| in planta transcription of a second subgenomic rna increases the complexity of the subgroup 2 luteovirus genome. | the genetic information of potato leafroll virus (plrv), a typical member of the subgroup 2 luteoviruses, is contained in a single-stranded (+) sense rna of approximately 5.9 kb. a single subgenomic rna (sgrna1) of approximately 2.3 kb has been characterized as the mrna for the 3' clustered viral open reading frames orf3, orf3/5 and orf4. here we demonstrate by northern blot analyses of polysomal rnas from plrv-infected solanum tuberosum and physalis floridana plants that, as with luteoviruses b ... | 1998 | 9421494 |
| p0 of beet western yellows virus is a suppressor of posttranscriptional gene silencing. | higher plants employ a homology-dependent rna-degradation system known as posttranscriptional gene silencing (ptgs) as a defense against virus infection. several plant viruses are known to encode proteins that can suppress ptgs. here we show that p0 of beet western yellows virus (bwyv) displays strong silencing suppressor activity in a transient expression assay based upon its ability to inhibit ptgs of green fluorescent protein (gfp) when expressed in agro-infiltrated leaves of nicotiana bentha ... | 2002 | 12050394 |
| distribution and molecular diversity of three cucurbit-infecting poleroviruses in china. | cucurbit aphid-borne yellows virus (cabyv) and melon aphid-borne yellows virus (mabyv) have been found to be associated with cucurbit yellowing disease in china. our report identifies for the first time a third distinct polerovirus, tentatively named suakwa aphid-borne yellows virus (sabyv), infecting suakwa vegetable sponge. to better understand the distribution and molecular diversity of these three poleroviruses infecting cucurbits, a total of 214 cucurbitaceous crop samples were collected fr ... | 2009 | 19664664 |
| a reinvestigation provides no evidence for sugar residues on structural proteins of poleroviruses and argues against a role for glycosylation of virus structural proteins in aphid transmission. | poleroviruses are strictly transmitted by aphids. glycosylation of turnip yellows virus (tuyv) was previously reported and this modification was supposed to be required for aphid transmission. using different approaches based on (i) a lectin-binding assay, (ii) use of specific complex glycan antibodies and (iii) mass spectrometry, we found no evidence that the structural proteins of tuyv and cucurbit aphid-borne yellow virus (cabyv) carry glycan residues. moreover, mutation of each of the four p ... | 2010 | 20416918 |
| phloem protein partners of cucurbit aphid borne yellows virus: possible involvement of phloem proteins in virus transmission by aphids. | poleroviruses are phytoviruses strictly transmitted by phloem-feeding aphids in a circulative and nonpropagative mode. during ingestion, aphids sample virions in sieve tubes along with sap. therefore, any sap protein bound to virions will be acquired by the insects and could potentially be involved in the transmission process. by developing in vitro virus-overlay assays on sap proteins collected from cucumber, we observed that approximately 20 proteins were able to bind to purified particles of ... | 2010 | 20459319 |
| detection and discrimination of members of the family luteoviridae by real-time pcr and sybr® greener™ melting curve analysis. | this study investigated the suitability of a two step real-time rt-pcr melting curve analysis as a tool for the detection and discrimination of nine species in the plant virus family luteoviridae, being soybean dwarf virus [sbdv], bean leafroll virus [blrv], beet chlorosis virus [bchv], beet mild yellowing virus [bmyv], beet western yellows virus [bwyv], cereal yellow dwarf virus-rpv [cydv-rpv], cucurbit aphid-borne yellows virus [cabyv], potato leafroll virus [plrv] and turnip yellows virus [tu ... | 2010 | 20933015 |
| incidence and molecular diversity of poleroviruses infecting cucurbit crops and weed plants in thailand. | overall, 244 samples of cucurbit crops with yellowing symptoms and selected weed species, from 15 provinces in thailand, were screened by rt-pcr using primers polero-cp-f and polero-cp-r. a total of 160 samples (~66%) were infected by poleroviruses. analysis of a 1.4 kb region covering the 3' rna-dependent rna polymerase (rdrp) gene, the intergenic non-coding region (incr), and the coat protein (cp), showed that four poleroviruses, namely, cucurbit aphid-borne yellows virus (cabyv), luffa aphid- ... | 2017 | 28352973 |
| pepo aphid-borne yellows virus: a new species in the genus polerovirus. | pepo aphid-borne yellows virus (pabyv) has been proposed as a putative representative of a new species in the genus polerovirus in the family luteoviridae. the genomes of two south african (sa) isolates of cucurbit-infecting pabyv were described in this record. total rna, extracted from a pattypan (cucurbita pepo l.) and a baby marrow (c. pepo l.) leaf samples, was subjected to next-generation sequencing (ngs) on the hiseq illumina platform. sanger sequencing was subsequently used to authenticat ... | 2017 | 27623838 |
| resistance to cucurbit aphid-borne yellows virus in melon accession tgr-1551. | the genetic control of resistance to cucurbit aphid-borne yellows virus (cabyv; genus polerovirus, family luteoviridae) in the tgr-1551 melon accession was studied through agroinoculation of a genetic family obtained from the cross between this accession and the susceptible spanish cultivar 'bola de oro'. segregation analyses were consistent with the hypothesis that one dominant gene and at least two more modifier genes confer resistance; one of these additional genes is likely present in the su ... | 2015 | 26075973 |
| elicitation of hypersensitive responses in nicotiana glutinosa by the suppressor of rna silencing protein p0 from poleroviruses. | plant disease resistance (r) proteins that confer resistance to viruses recognize viral gene products with diverse functions, including viral suppressors of rna silencing (vsrs). the p0 protein from poleroviruses is a vsr that targets the argonaute1 (ago1) protein for degradation, thereby disrupting rna silencing and antiviral defences. here, we report resistance against poleroviruses in nicotiana glutinosa directed against turnip yellows virus (tuyv) and potato leafroll virus (plrv). the p0 pro ... | 2015 | 25187258 |
| viruses of cucurbit crops in the mediterranean region: an ever-changing picture. | cucurbit crops may be affected by at least 28 different viruses in the mediterranean basin. some of these viruses are widely distributed and cause severe yield losses while others are restricted to limited areas or specific crops, and have only a negligible economic impact. a striking feature of cucurbit viruses in the mediterranean basin is their always increasing diversity. indeed, new viruses are regularly isolated and over the past 35 years one "new" cucurbit virus has been reported on avera ... | 2012 | 22682166 |
| genetic diversity and potential vectors and reservoirs of cucurbit aphid-borne yellows virus in southeastern spain. | the genetic variability of a cucurbit aphid-borne yellows virus (cabyv) (genus polerovirus, family luteoviridae) population was evaluated by determining the nucleotide sequences of two genomic regions of cabyv isolates collected in open-field melon and squash crops during three consecutive years in murcia (southeastern spain). a phylogenetic analysis showed the existence of two major clades. the sequences did not cluster according to host, year, or locality of collection, and nucleotide similari ... | 2013 | 23802870 |
| the complete genomic sequence of pepper yellow leaf curl virus (pylcv) and its implications for our understanding of evolution dynamics in the genus polerovirus. | we determined the complete sequence and organization of the genome of a putative member of the genus polerovirus tentatively named pepper yellow leaf curl virus (pylcv). pylcv has a wider host range than tobacco vein-distorting virus (tvdv) and has a close serological relationship with cucurbit aphid-borne yellows virus (cabyv) (both poleroviruses). the extracted viral rna was subjected to solid next-generation sequence analysis and used as a template for reverse transcription synthesis, which w ... | 2013 | 23936244 |
| molecular diversity of poleroviruses infecting cucurbit crops in four countries reveals the presence of members of six distinct species. | when 66 cucurbit samples with yellowing symptoms from fields in mali, the philippines, thailand and uzbekistan were screened by rt-pcr using universal polerovirus primers, 21 were identified as harboring polerovirus rna. when these 21 samples were screened with specific primers for the known cucurbit-infecting poleroviruses, suakwa aphid-borne yellows virus and a recombinant strain of cucurbit aphid-borne yellows virus were detected for the first time in the philippines and thailand. however, se ... | 2014 | 24337720 |
| interfamilial recombination between viruses led to acquisition of a novel translation-enhancing rna element that allows resistance breaking. | many plant viruses depend on functional rna elements, called 3'-utr cap-independent translation enhancers (3'-cites), for translation of their rnas. in this manuscript we provide direct proof for the existing hypothesis that 3'-cites are modular and transferable by recombination in nature, and that this is associated with an advantage for the created virus. by characterizing a newly identified melon necrotic spot virus (mnsv; tombusviridae) isolate, which is able to overcome eukaryotic translati ... | 2014 | 24372390 |
| both structural and non-structural forms of the readthrough protein of cucurbit aphid-borne yellows virus are essential for efficient systemic infection of plants. | cucurbit aphid-borne yellows virus (cabyv) is a polerovirus (luteoviridae family) with a capsid composed of the major coat protein and a minor component referred to as the readthrough protein (rt). two forms of the rt were reported: a full-length protein of 74 kda detected in infected plants and a truncated form of 55 kda (rt*) incorporated into virions. both forms were detected in cabyv-infected plants. to clarify the specific roles of each protein in the viral cycle, we generated by deletion a ... | 2014 | 24691251 |
| spatio-temporal dynamics of viruses are differentially affected by parasitoids depending on the mode of transmission. | relationships between agents in multitrophic systems are complex and very specific. insect-transmitted plant viruses are completely dependent on the behaviour and distribution patterns of their vectors. the presence of natural enemies may directly affect aphid behaviour and spread of plant viruses, as the escape response of aphids might cause a potential risk for virus dispersal. the spatio-temporal dynamics of cucumber mosaic virus (cmv) and cucurbit aphid-borne yellows virus (cabyv), transmitt ... | 2012 | 23202516 |
| control of insect vectors and plant viruses in protected crops by novel pyrethroid-treated nets. | long-lasting insecticide-treated nets (llitns) constitute a novel alternative that combines physical and chemical tactics to prevent insect access and the spread of insect-transmitted plant viruses in protected enclosures. this approach is based on a slow-release insecticide-treated net with large hole sizes that allow improved ventilation of greenhouses. the efficacy of a wide range of llitns was tested under laboratory conditions against myzus persicae, aphis gossypii and bemisia tabaci. two n ... | 2015 | 25404196 |
| biological and molecular characterization of a korean isolate of cucurbit aphid-borne yellows virus infecting cucumis species in korea. | surveys of yellowing viruses in plastic tunnels and in open field crops of melon (cucumis melo cultivar catalupo), oriental melon (c. melo cultivar oriental melon), and cucumber (c. sativus) were carried out in two melon-growing areas in 2014, korea. severe yellowing symptoms on older leaves of melon and chlorotic spots on younger leaves of melon were observed in the plastic tunnels. the symptoms were widespread and included initial chlorotic lesions followed by yellowing of whole leaves and thi ... | 2015 | 26673519 |
| studies on interaction of cucurbit aphid-borne yellow virus proteins using yeast two-hybrid system and bimolecular fluorescence complementation. | in this article, yeast two-hybrid system (yths) and bimolecular fluorescence complementation (bifc) were used to analyze the interactions of cucurbit aphid-borne yellows virus (cabyv)-encoded proteins. p0, p1, p1-2, p3, p4, and p5 were tested by yths in all possible pairwise combinations, and only p3/p3 interaction was detected. results obtained by bifc further confirmed the self-interaction of p3, and the subcellular localization of reconstituted yfp fluorescence was observed mainly in nuclei o ... | 2011 | 21978157 |
| a novel strain of beet western yellows virus infecting sugar beet with two distinct genotypes differing in the 5'-terminal half of genome. | the complete genomic sequences of two distinct beet western yellows virus (bwyv) genotypes infecting sugar beet in beijing, named as bwyv-bj(a) and bwyv-bj(b) (genbank accession number hm804471, hm804472, respectively), were determined by rt-pcr sub-cloning approach. bwyv-bj(a) and bwyv-bj(b) were 5674 and 5626nt in length, respectively. bwyv-bj(b) was 48nt shorter than bwyv-bj(a) in the regions 1589-1615 and 1629-1649nt. sequence alignment analysis showed that the full length of bwyv-bj(a) and ... | 2010 | 21104195 |
| molecular characterization of geographically different cucurbit aphid-borne yellows virus isolates. | cucurbit aphid-borne yellows virus (cabyv) causes yellowing symptoms in cucurbit crops worldwide. in this work, the sequences for the coat protein (cp) gene for cabyv isolates from iran and slovakia are reported for the first time. three iranian isolates shared 96.2-99.3% and the slovak isolate 98.9% of nucleotide identity in comparison with the reference french cabyv-n isolate. phylogenetic analysis showed that the sequence of cp gene of examined cabyv isolates differed only slightly. relativel ... | 2009 | 19301954 |
| the p0 gene of sugarcane yellow leaf virus encodes an rna silencing suppressor with unique activities. | the sugarcane yellow leaf virus (scylv) p0, a member of the highly heterologous proteins of poleroviruses, is a suppressor of posttranscriptional gene silencing (ptgs) and has additional activities not seen in other p0 proteins. the p0 protein in previously tested poleroviruses (beet western yellows virus and cucurbit aphid-borne yellows virus), suppresses local, but not systemic, ptgs induced by both sense gfp and inverted repeat gf using its f-box-like domain to mediate destabilization of the ... | 2009 | 19046592 |
| occurrence and distribution of ten viruses infecting cucurbit plants in guilan province, iran. | during the 2006 and 2007 growing seasons, a systematic survey was conducted in open-field of melon (cucumis melo l.), cucumber (c. sativus l.), squash (cucurbita sp.), and watermelon (citrulus lanatus l.) crops in 16 major cucurbit-growing areas of guilan province in iran. symptomatic leaf samples were collected and screened by double-antibody sandwich elisa (das-elisa) or rt-pcr to detect zucchini yellow mosaic virus (zymv), watermelon mosaic virus (wmv), cucurbit aphid-borne yellows virus (cab ... | 2008 | 18564898 |
| complete sequence analysis reveals two distinct poleroviruses infecting cucurbits in china. | the complete rna genomes of a chinese isolate of cucurbit aphid-borne yellows virus (cabyv-chn) and a new polerovirus tentatively referred to as melon aphid-borne yellows virus (mabyv) were determined. the entire genome of cabyv-chn shared 89.0% nucleotide sequence identity with the french cabyv isolate. in contrast, nucleotide sequence identities between mabyv and cabyv and other poleroviruses were in the range of 50.7-74.2%, with amino acid sequence identities ranging from 24.8 to 82.9% for in ... | 2008 | 18414972 |
| the polerovirus minor capsid protein determines vector specificity and intestinal tropism in the aphid. | aphid transmission of poleroviruses is highly specific, but the viral determinants governing this specificity are unknown. we used a gene exchange strategy between two poleroviruses with different vectors, beet western yellows virus (bwyv) and cucurbit aphid-borne yellows virus (cabyv), to analyze the role of the major and minor capsid proteins in vector specificity. virus recombinants obtained by exchanging the sequence of the readthrough domain (rtd) between the two viruses replicated in plant ... | 2005 | 16014930 |
| posterior midgut and hindgut are both sites of acquisition of cucurbit aphid-borne yellows virus in myzus persicae and aphis gossypii. | members of the family luteoviridae ('luteovirids') rely strictly on aphid vectors for plant-to-plant transmission. this interaction operates according to a persistent and circulative manner, which implies that the virions are being endocytosed and exocytosed across two epithelial barriers (alimentary tract and accessory salivary glands) in the vector's body. in several luteovirid-aphid vector species combinations, the route of virions in the insect has been investigated ultrastructurally by tran ... | 2003 | 14645929 |
| analysis of an rna-seq strand-specific library from an east timorese cucumber sample reveals a complete cucurbit aphid-borne yellows virus genome. | analysis of an rna-seq library from cucumber leaf rna extracted from a fast technology for analysis of nucleic acids (fta) card revealed the first complete genome of cucurbit aphid-borne yellows virus (cabyv) from east timor. we compare it with 35 complete cabyv genomes from other world regions. it most resembled the genome of the south korean isolate hd118. | 2017 | 28495776 |
| impact of vat resistance in melon on viral epidemics and genetic structure of virus populations. | cultivar choice is at the heart of cropping systems and resistant cultivars should be at the heart of disease management strategies whenever available. they are the easiest, most efficient and environmentally friendly way of combating viral diseases at the farm level. among the melon genetic resources, vat is a unique gene conferring resistance to both the melon aphid aphis gossypii and the viruses it carries. the 'virus side' of this pleiotropic phenotype is seldom regarded as an asset for viru ... | 2017 | 28587865 |