| molecular interactions between porcine and human gammaherpesviruses: implications for xenografts? | reactivation of latent herpesviruses is an important cause of morbidity and mortality in human transplantation. this issue might be further complicated in the case of xenotransplantation. zoonotic viruses could reactivate and replicate in the transplanted tissue, and interactions with homologous human viruses could take place. since the pig is a favoured animal as donor of organs for human transplants, we analysed the possibility of interactions between porcine and human herpesviruses. porcine l ... | 2006 | 16768724 |
| gene expression of porcine lymphotrophic herpesvirus-1 in miniature swine with posttransplant lymphoproliferative disorder. | porcine lymphotropic herpesvirus-1 (plhv-1) is a gamma-herpesvirus related to epstein-barr virus (ebv) and associated with development of posttransplant lymphoproliferative disorder (ptld) following allogeneic stem cell or spleen transplantation in miniature swine. oligonucleotide microarrays were designed based on known open reading frames (orfs) of plhv-1. expression was compared by cohybridization of cdna from lymph nodes of plhv-1+ swine after allogeneic spleen transplantation between either ... | 2007 | 17220799 |
| discovery of herpesviruses in bats. | seven novel gammaherpesviruses (ghv) and one novel betaherpesvirus were discovered in seven different european bat species (order chiroptera, family vespertilionidae) with a pan-herpesvirus pcr assay, targeting the dna polymerase (dpol) gene. the sequences of six bat ghv were similarly related to members of the gammaherpesvirus genera percavirus and rhadinovirus. the seventh ghv was related to the porcine lymphotropic herpesvirus 1 (genus macavirus). the betaherpesvirus appeared to be a distant ... | 2007 | 17872515 |
| posttransplant lymphoproliferative disease after allogeneic transplantation of the spleen in miniature swine. | spleen transplantation (sptx) was performed in miniature swine across full major histocompatibility complex barriers to study the tolerogenic effect of the spleen. this study describes the development of posttransplant lymphoproliferative disease (ptld) after allogeneic sptx. recipient pigs underwent whole body irradiation (100 cgy), thymic irradiation (700 cgy), and native splenectomy (day 0), and received a 45-day course of intravenous cyclosporine (trough level 400-800 ng/ml). after sptx, two ... | 2004 | 15280692 |
| a novel porcine gammaherpesvirus. | a novel porcine gammaherpesvirus was detected in the blood of domestic pigs by pcr. with degenerate-primer pcr and subsequent long-distance pcr approaches a 60-kbp genome stretch was amplified. sequence analysis revealed the presence of the gammaherpesvirus orfs 03 to 46 as well as a putative chemokine receptor and a v-bcl-2 gene. the 60-kbp sequence was compared with the corresponding sequence of the porcine lymphotropic herpesvirus 1 (plhv-1) published recently and the sequence of plhv-2, whic ... | 2003 | 12706081 |
| sequence analysis of the genome of porcine lymphotropic herpesvirus 1 and gene expression during posttransplant lymphoproliferative disease of pigs. | the porcine lymphotropic herpesvirus 1 (plhv-1), the first gammaherpesvirus of pigs, has been detected at a high prevalence in healthy pig populations. a porcine gammaherpesvirus has also been detected at high copy numbers in animals suffering from posttransplant lymphoproliferative disease (ptld). while human ptld is a ebv-associated complication following clinical transplantation, porcine ptld is a disease recently described in pigs undergoing experimental allogeneic hematopoietic stem cell tr ... | 2002 | 12009880 |
| the porcine lymphotropic herpesvirus 1 encodes functional regulators of gene expression. | the porcine lymphotropic herpesviruses (plhv) are discussed as possible risk factors in xenotransplantation because of the high prevalence of plhv-1, plhv-2 and plhv-3 in pig populations world-wide and the fact that plhv-1 has been found to be associated with porcine post-transplant lymphoproliferative disease. to provide structural and functional knowledge on the plhv immediate-early (ie) transactivator genes, the central regions of the plhv genomes were characterized by genome walking, sequenc ... | 2007 | 16979210 |