| ketopantoyl-lactone reductase from candida parapsilosis: purification and characterization as a conjugated polyketone reductase. | ketopantoyl-lactone reductase (2-dehydropantoyl-lactone reductase, ec 1.1.1.168) was purified and crystallized from cells of candida parapsilosis ifo 0708. the enzyme was found to be homogeneous on ultracentrifugation, high-performance gel-permeation liquid chromatography and sds-polyacrylamide gel electrophoresis. the relative molecular mass of the native and sds-treated enzyme is approximately 40,000. the isoelectric point of the enzyme is 6.3. the enzyme was found to catalyze specifically the ... | 1989 | 2644973 |
| a novel fungal enzyme, nadph-dependent carbonyl reductase, showing high specificity to conjugated polyketones. purification and characterization. | a novel enzyme which specifically catalyzes the reduction of conjugated polyketones was purified to homogeneity from cells of mucor ambiguus aku 3006. the enzyme has a strict requirement for nadph and irreversibly reduces a number of quinones such as p-benzoquinone, alpha-naphthoquinone and acenaphthenequione. the enzyme also reduces polyketones such as isatin and ketopantoyl lactone, and their derivatives. the apparent km values for isatin and ketopantoyl lactone are 49.9 microm and 714 microm, ... | 1988 | 3286260 |