identification of alternatives for the management of foliar nematodes in floriculture.the foliar nematodes, aphelenchoides spp, have emerged as important pests of ornamentals in north america during the last decade. due to the ban on the use of potentially toxic pesticides, there are currently no nematicides registered to manage foliar nematodes on ornamentals. therefore, we have evaluated a biological [burkholderia cepacia (syn pseudomonas cepacia)], two plant products [clove (syzygium aromaticum) extract and nimbecidine (azadirachtin)] and twelve chemical pesticides registered ...200211997971
influence of lysobacter enzymogenes strain c3 on nematodes.chitinolytic microflora may contribute to biological control of plant-parasitic nematodes by causing decreased egg viability through degradation of egg shells. here, the influence of lysobacter enzymogenes strain c3 on caenorhabditis elegans, heterodera schachtii, meloidogyne javanica, pratylenchus penetrans, and aphelenchoides fragariae is described. exposure of c. elegans to l. enzymogenes strain c3 on agar resulted in almost complete elimination of egg production and death of 94% of hatched j ...200619259452
efficacy of insecticides for control of aphelenchoides fragariae and ditylenchus dipsaci in flowering perennial ornamentals.the effects of abamectin b1, diazinon, and methiocarb insecticides on aphelenchoides fragariae and ditylenchus dipsaci in lamium maculatum, phlox subulata, rhododendron indicum, and begonia x tuberhybrida were determined in a series of greenhouse experiments. abamectin at 0.005 or 0.011 g a.i./liter (0.3 or 0.6 ml/liter avid 0.15 ec), diazinon at 0.62 or 1.87 g a.i./liter (2.6 or 7.8 ml/liter knoxout gh), or methiocarb at 3.5 g a.i./liter (4.7 g/liter mesurol) were applied in two to six weekly o ...199919270930
refinement of hot water treatment for management of aphelenchoides fragariae in strawberry.the effects of hot water treatments on a california population of the foliar nematode, aphelenchoides fragariae, and on five strawberry cuttivars ('chandler', 'douglas', 'fern', 'pajaro', and 'selva') were assessed in laboratory and greenhouse tests. nematodes extracted from fern leaves were placed in water maintained at 44.4, 46.1,47.7, or 49.4 c for different time periods. exposure periods of 15, 5, 4, and 2 minutes were required to produce 100% mortality at 44.4, 46.1, 47.7, and 49.4 c, respe ...199319279842
interrelationship of aphelenchoides fragariae and xanthomonas begoniae on rieger begonia. 197419308125
induction of glutaredoxin expression in response to desiccation stress in the foliar nematode aphelenchoides fragariae.desiccation tolerance plays an important role in the overwinter survival of the foliar nematode aphelenchoides fragariae. survival rates of a. fragariae were compared with those of the anhydrobiotic soil-dwelling nematode aphelenchus avenae after desiccation (90% rh), cold (4°c) and osmotic (500 mm sucrose) stress treatments. a. fragariae formed aggregates during desiccation and showed higher survival rates than a. avenae under desiccation and osmotic stress. analysis of transcripts with illumin ...201223483835
ruscus hypophyllum: a new host for aphelenchoides fragariae.aphelenchoides fragariae was isolated from the phylloclades of the ornamental plant ruscus hypophyllum (liliaceae). rotylenchus buxophilus, scutellonema brachyurum, and meloidogyne were identified as the most common plant-parasitic nematodes in the soil near the roots. the pathology and life history of a. fragariae were closely related to the climate. to our knowledge, this is the first report of r. hypophyllum as a host of plant-parasitic nematodes.199319279774
effectiveness of a hot water drench for the control of foliar nematodes aphelenchoides fragariae in floriculture.effectiveness of a hot water drench for the control of aphelenchoides fragariae infesting hosta (hosta sp.) and ferns (matteuccia pensylvanica) was studied. drenching with hot water at 70 degrees c and 90 degrees c in october reduced (p < 0.05) a. fragariae in the soil but not in the leaves relative to the control (25 degrees c) 300 days after treatment (dat). plants drenched with 90 degrees c water had lower numbers of nematode-infected leaves per plant than those treated with 25 degrees c and ...200419262787
conventional and pcr detection of aphelenchoides fragariae in diverse ornamental host plant species.a pcr-based diagnostic assay was developed for early detection and identification of aphelenchoides fragariae directly in host plant tissues using the species-specific primers afragfl and afragrl that amplify a 169-bp fragment in the internal transcribed spacer (its1) region of ribosomal dna. these species-specific primers did not amplify dna from aphelenchoides besseyi or aphelenchoides ritzemabosi. the pcr assay was sensitive, detecting a single nematode in a background of plant tissue extract ...200719259510
infection behavior and overwintering survival of foliar nematodes, aphelenchoides fragariae, on hosta.we studied the pathogenicity and overwintering survival of the foliar nematode, aphelenchoides fragariae, infecting hosta spp. nematodes applied to either lower or upper sides of noninjured and injured hosta leaves were able to infect and produce typical symptoms on nine cultivars. leaves of only four cultivars (borschi, fragrant blue, patomic pride, and olive bailey langdon) showed no symptoms of nematode infection. the nematodes overwintered as juveniles and adults in soil, dry leaves, and dor ...200619259438
nematicidal activity of fluensulfone against some migratory nematodes under laboratory conditions.the fluoroalkenyl fluensulfone, known to have strong nematicidal activity against meloidogyne spp. (root-knot nematodes), was evaluated in vitro and in soil against the migratory nematodes bursaphelenchus xylophilus, aphelenchoides besseyi, aphelenchoides fragariae, ditylenchus dipsaci, pratylenchus penetrans, pratylenchus thornei and xiphinema index.201424452922
population dynamics and dispersal of aphelenchoides fragariae in nursery-grown lantana.population dynamics of aphelenchoides fragariae were assessed over three growing seasons and during overwintering for naturally-infected, container-grown lantana (latana camara) plants in a north carolina nursery. during the growing season, the foliar nematode population in symptomatic leaves peaked in july each year then remained above 100 nematodes/g fresh weight into late summer. foliar nematodes were also detected in asymptomatic and abscised leaves. results suggest that leaves infected with ...201022736867
differential expression of a β-1,4-endoglucanase induced by diet change in the foliar nematode aphelenchoides fragariae.we identified and characterized a β-1,4-endoglucanase, afr-eng-1, in the foliar nematode aphelenchoides fragariae that is differentially expressed when the nematode feeds on fungi or plants. when individuals from hosta plants were transferred to a fungus culture, expression of the enzyme decreased 1,812-fold after five generations on the fungus diet. afr-eng-1 was readily detected in the genome of 75% of nematodes from the plant population but only in 38% of the diet-changed population. the gene ...201222779746
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