Publications

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post-mortem isolation of vibrio alginolyticus from an atlantic white-sided dolphin (lagenorynchus acutus).post-mortem examination of a stranded atlantic white-sided dolphin (lagenorynchus acutus) revealed congested lungs, sanguinous fluid in the peritoneal cavity, and an oral wound. histopathologic findings were acute necrotizing hepatitis and acute focal bronchopneumonia. vibrio alginolyticus was isolated from the blood and other organs.19807411736
isolation of brucella species from cetaceans, seals and an otter.brucella organisms which differed from the recognised species of the genus, were isolated from nine seals, eight cetaceans and one otter. a method is described for the isolation of brucella species from sea mammals and the first isolations of brucella species are recorded from an atlantic white-sided dolphin (lagenorhynchus acutus), two striped dolphins (stenella coeuleoalba), a hooded seal (cystophora cristata), a grey seal (halichoerus grypus) and a european otter (lutra lutra). there were dif ...19968799984
[viruses of whales and dolphins].dna- and rna-genome viruses of whales and dolphins belong to families poxviridae, herpesviridae, adenoviridae, orthomyxoviridae, paramyxoviridae, togaviridae, picornaviridae. virological, serological and pathomorphological signs of infection have been registered in odontoceti (bottle-nosed dolphin, atlantic white-sided dolphin, striped dolphin, harbona porpoise, white-beaked dolphin, common dolphin, sperm whale, pilot whale, white whale) and musticeti (sei whale, fin whale, gray whale, and bowhe ...19969044706
isolation and characterization of a helicobacter sp. from the gastric mucosa of dolphins, lagenorhynchus acutus and delphinus delphis.gastric ulcerations in dolphins have been reported for decades. some of these lesions were associated with parasitic infections. however, cases of nonparasitic gastric ulcers with no clearly defined etiology also have been reported in wild and captive dolphins. considerable speculation exists as to whether dolphins have helicobacter-associated gastritis and peptic ulcer disease. the stomachs of seven stranded atlantic white-sided dolphins, lagenorhynchus acutus, and 1 common dolphin, delphinus d ...200011055919
prevalence of sarcocystis sp. in stranded atlantic white-sided dolphins (lagenorhynchus acutus).in january 1998 and 1999, two mass strandings of dolphins occurred in wellfleet, massachusetts. the strandings were composed of 97 and 53 animals, respectively. tissues from 35 atlantic white-sided dolphins (lagenorhynchus acutus) from the 1998 stranding and 52 from the 1999 stranding were examined histologically. in the 1998 stranding, unidentified protozoal tissue cysts were seen in skeletal muscle from 11 of 28 (39%) dolphins. in addition, two dolphins had a protozoal tissue cyst in cardiac m ...200212038128
characterization of a cetacean aromatase (cyp19) and the phylogeny and functional conservation of vertebrate aromatase.aromatase (cytochrome p450 19, cyp19, p450arom) is the enzyme responsible for the production of estrogens, hormones critical for development and reproduction. aromatase was sequenced from a white-sided dolphin (lagenorhynchus acutus) ovary, transiently transfected into hek 293 cells, and the expressed protein was characterized for aromatase activity in the presence of androstenedione and testosterone and after exposure to the aromatase inhibitor letrazole. the kms for androstenedione and testost ...200515596073
identification of highly brominated analogues of q1 in marine mammals.three novel halogenated organic compounds (hocs) have been identified in the blubber of marine mammals from coastal new england with the molecular formulae c(9)h(3)n(2)br(6)cl, c(9)h(3)n(2)br(7), and c(9)h(4)n(2)br(5)cl. they were identified using high and low resolution gas chromatography mass spectrometry (gcms) and appear to be highly brominated analogues of q1, a heptachlorinated hoc suspected to be naturally produced. these compounds were found in atlantic white sided dolphin (lagenorhynchu ...200616517037
isolation of brucella species from a diseased atlanto-occipital joint of an atlantic white-sided dolphin (lagenorhynchus acutus). 200717586794
modular organizations of novel cetacean papillomaviruses.the phylogenetic position of cetacean papillomaviruses (pvs: omikron-pvs and upsilon-pvs) varies depending on the region of the genome analysed. they cluster together with alpha-pvs when analysing early genes and with xi-pvs and phi-pvs when analysing late genes. we cloned and sequenced the complete genomes of five novel pvs, sampled from genital and oesophageal lesions of free-ranging cetaceans: delphinus delphis (ddpv1), lagenorhynchus acutus (ttpv3 variant), and phocoena phocoena (pphpv1, pph ...201021195783
entry and elimination of marine mammal brucella spp. by hooded seal (cystophora cristata) alveolar macrophages in vitro.a high prevalence of brucellapinnipedialis serology and bacteriology positive animals has been found in the northeast atlantic stock of hooded seal (cystophoracristata); however no associated gross pathological changes have been identified. marine mammal brucellae have previously displayed different infection patterns in human and murine macrophages. to investigate if marine mammal brucella spp. are able to invade and multiply in cells originating from a presumed host species, we infected alveol ...201323936159
application of the luminometric methylation assay to ecological species: tissue quality requirements and a survey of dna methylation levels in animals.the luminometric methylation assay (luma) measures global dna methylation. luma depends on digestion of dna with methyl-sensitive and methyl-insensitive restriction enzymes, followed by pyrosequencing. until recently, luma has been principally used for biomedical research. here, we use chickens as a model to investigate sample quality issues relating to luma and then apply the method to ecological species. first, we assessed the effect of tissue storage conditions on dna methylation values. this ...201424576185
methoxylated polybrominated diphenyl ethers (meo-pbdes) are major contributors to the persistent organobromine load in sub-arctic and arctic marine mammals, 1986-2009.a selection of meo-bde and bde congeners were analyzed in pooled blubber samples of pilot whale (globicephala melas), ringed seal (phoca hispida), minke whale (balaenoptera acutorostrata), fin whale (balaenoptera physalus), harbor porpoise (phocoena phocoena), hooded seal (cystophora cristata), and atlantic white-sided dolphin (lagenorhynchus acutus), covering a time period of more than 20 years (1986-2009). the analytes were extracted and cleaned-up using open column extraction and multi-layer ...201222225820
polychlorinated naphthalenes (pcns) in sub-arctic and arctic marine mammals, 1986-2009.a selection of pcn congeners was analyzed in pooled blubber samples of pilot whale (globicephala melas), ringed seal (phoca hispida), minke whale (balaenoptera acutorostrata), fin whale (balaenoptera physalus), harbour porpoise (phocoena phocoena), hooded seal (cystophora cristata) and atlantic white-sided dolphin (lagenorhynchus acutus), covering a time period of more than 20 years (1986-2009). a large geographical area of the north atlantic and arctic areas was covered. pcn congeners 48, 52, 5 ...201222356752
meals on wheels? a decade of megafaunal visual and acoustic observations from offshore oil & gas rigs and platforms in the north and irish seas.a decade of visual and acoustic detections of marine megafauna around offshore oil & gas (o&g) installations in the north and irish seas are presented. marine megafauna activity was monitored visually and acoustically by joint nature conservation committee (jncc) qualified and experienced marine mammal observers (mmo) and passive acoustic monitoring (pam) operators respectively, with real-time towed pam in combination with industry standard software, pamguard. monitoring was performed during rou ...201627078153
helicobacter cetorum infection in striped dolphin (stenella coeruleoalba), atlantic white-sided dolphin (lagenorhynchus acutus), and short-beaked common dolphin (delphinus delphus) from the southwest coast of england.helicobacter infection in cetaceans was first reported from the us in 2000 when the isolation of a novel helicobacter species was described from two atlantic white-sided dolphins (lagenorhynchus acutus). since then, helicobacter species have been demonstrated in cetaceans and pinnipeds from around the world. since 1990, the animal health and veterinary laboratories agency polwhele, truro, has been involved in the uk cetacean strandings investigation programme to establish the cause of death of c ...201424807181
meningoencephalitis and arthritis associated with brucella ceti in a short-beaked common dolphin (delphinus delphis).brucella species infection in marine mammal species has been reported to have a global distribution. in 2007, the description of brucella ceti was published and formally adopted for those isolates originating from cetaceans and pathologic lesions similar to those seen in terrestrial mammals infected with brucella spp. have been associated with its isolation. brucella ceti infection specific to the central nervous system has been described in two species of cetacean: striped dolphins (stenella co ...201323778612
first report of brucella ceti-associated meningoencephalitis in a long-finned pilot whale globicephala melas.fatal brucella ceti infection with histological lesions specific to the central nervous system has been described in only 3 species of cetaceans: striped dolphins stenella coeruleoalba, atlantic white-sided dolphins lagenorhynchus acutus and short-beaked common dolphins delphinus delphis. this paper describes the first report of a b. ceti-associated meningoencephalitis in a long-finned pilot whale globicephala melas, showing the increasing range of species susceptibility. brucella was recovered ...201526503778
increasing levels of long-chain perfluorocarboxylic acids (pfcas) in arctic and north atlantic marine mammals, 1984-2009.temporal variations in concentrations of perfluorinated carboxylic acids (pfcas) and sulfonic acids (pfsas), including perfluorooctane sulfonate (pfos) and perfluorooctanoate (pfoa) structural isomers, were examined in livers of pilot whale (globicephala melas), ringed seal (phoca hisida), minke whale (balaenoptera acutorostrata), harbor porpoise (phocoena phocoena), hooded seal (cystophora cristata), atlantic white-sided dolphin (lagenorhynchus acutus) and in muscle tissue of fin whales (balaen ...201222051347
static inflation and deflation pressure-volume curves from excised lungs of marine mammals.excised lungs from eight marine mammal species [harp seal (pagophilus groenlandicus), harbor seal (phoca vitulina), gray seal (halichoerus grypush), atlantic white-sided dolphin (lagenorhynchus acutus), common dolphin (delphinus delphis), risso's dolphin (grampus griseus), long-finned pilot whale (globicephala melas) and harbor porpoise (phocoena phocoena)] were used to determine the minimum air volume of the relaxed lung (mav, n=15), the elastic properties (pressure-volume curves, n=24) of the ...201122031747
mortality trends of stranded marine mammals on cape cod and southeastern massachusetts, usa, 2000 to 2006.to understand the cause of death of 405 marine mammals stranded on cape cod and southeastern massachusetts between 2000 and 2006, a system for coding final diagnosis was developed and categorized as (1) disease, (2) human interaction, (3) mass-stranded with no significant findings, (4) single-stranded with no significant findings, (5) rock and/or sand ingestion, (6) predatory attack, (7) failure to thrive or dependent calf or pup, or (8) other. the cause of death for 91 animals could not be dete ...201020225675
organohalogen contaminants and metabolites in cerebrospinal fluid and cerebellum gray matter in short-beaked common dolphins and atlantic white-sided dolphins from the western north atlantic.concentrations of several congeners and classes of organohalogen contaminants (ohcs) and/or their metabolites, namely organochlorine pesticides (ocs), polychlorinated biphenyls (pcbs), hydroxylated-pcbs (oh-pcbs), methylsulfonyl-pcbs (meso(2)-pcbs), polybrominated diphenyl ether (pbde) flame retardants, and oh-pbdes, were measured in cerebrospinal fluid (csf) of short-beaked common dolphins (n = 2), atlantic white-sided dolphins (n = 8), and gray seal (n = 1) from the western north atlantic. in ...200919375836
distribution patterns suggest biomagnification of halogenated 1'-methyl-1,2'-bipyrroles (mbps).the halogenated 1'-methyl-1,2'-bipyrroles (mbps) are a suite of marine natural products that have been detected in marine mammals worldwide. although their concentrations are similar to persistent organic pollutants that biomagnify, such as 2,2',4,4',5,5'-hexachlorobiphenyl (cb-153), it is notyet clearthat these natural products also biomagnify. here we analyze mbps and cb-153 isolated from the blubber and liver of marine mammals stranded on the eastern coast of massachusetts. four odontocete sp ...200919209594
comparison of diagnostic techniques for helicobacter cetorum infection in wild atlantic bottlenose dolphins (tursiops truncatus).helicobacter cetorum sp. nov. has been cultured from the stomach of atlantic white-sided dolphins (lagenorhynchus acutus) and the feces of pacific white-sided (l. obliquidens) and atlantic bottlenose (tursiops truncatus) dolphins and a beluga whale (delphinapterus leucas). h. cetorum has high homology to helicobacter pylori as shown by 16s rrna sequencing, and h. cetorum infection has been associated with gastritis and clinical signs in cetaceans. because the prevalence of h. cetorum in wild pop ...200312843010
marine mammals from northeast atlantic: relationship between their trophic status as determined by delta13c and delta15n measurements and their trace metal concentrations.the relationship between trophic position through delta13c and delta15n and trace metal concentrations (zn, cd, cu and hg) was investigated in the tissues of six marine mammal species from the northeast atlantic: striped dolphin stenella coeruleoalba, common dolphin, delphinus delphis, atlantic white-sided dolphin lagenorhynchus acutus, harbour porpoise phocoena phocoena, white beaked-dolphin lagenorhynchus albirostris, grey seal halichoerus grypus stranded on french channel and irish coasts. wh ...200312738219
helicobacter cetorum sp. nov., a urease-positive helicobacter species isolated from dolphins and whales.a novel helicobacter with the proposed name helicobacter cetorum, sp. nov. (type strain mit 99-5656; genbank accession number af 292378), was cultured from the main stomach of two wild, stranded atlantic white-sided dolphins (lagenorhynchus acutus) and from the feces of three captive cetaceans (a pacific white-sided dolphin [lagenorhynchus obliquidens]; an atlantic bottlenose dolphin [tursiops truncatus]; and a beluga whale [delphinapterus leucas]). the infected captive cetaceans were either sub ...200212454148
concentrations of chlorinated hydrocarbons and trace elements in marine mammal tissues archived in the u.s. national biomonitoring specimen bank.the u.s. national biomonitoring specimen bank (nbsb) provides for the long term storage of well documented and preserved specimens representing several types of environmental matrices. a major part of this inventory consists of marine mammal tissues (e.g., blubber, liver, kidney, and muscle). within the nbsb selected specimens are periodically analyzed for chlorinated hydrocarbons and trace elements. although only 20% of the 560 marine mammal specimens in the nbsb have been analyzed, the databas ...19979159906
identification of mercury and other metals complexes with metallothioneins in dolphin liver by hydrophilic interaction liquid chromatography with the parallel detection by icp ms and electrospray hybrid linear/orbital trap ms/ms.a novel analytical procedure for the identification of metal (hg, cd, cu, zn) complexes with individual metallothionein (mt) isoforms in biological tissues by electrospray ms/ms was developed. the sample preparation was reduced to three rapid steps: the two-fold dilution of the sample cytosol with acetonitrile, the recovery of the supernatant containing mt-complexes by centrifugation and its concentration under nitrogen flow. the replacement of reversed phase hplc by hydrophilic interaction lc ( ...201222456936
hemoglobin as a major binding protein for methylmercury in white-sided dolphin liver.as methylmercury (mehg) can be bioaccumulated and biomagnified in the trophic web, its toxicity for marine mammals is of major concern. mercury speciation in marine biota has been widely studied, mainly focused on the discrimination and quantification of inorganic hg and mehg. less attention has been paid to the interactions of hg with biomolecules and the characterization of its specific binding, which play a key role in metabolic pathways controlling its uptake, transformation, and toxicity. i ...201423942567
a genomewide catalogue of single nucleotide polymorphisms in white-beaked and atlantic white-sided dolphins.the field of population genetics is rapidly moving into population genomics as the quantity of data generated by high-throughput sequencing platforms increases. in this study, we used restriction-site-associated dna sequencing (radseq) to recover genomewide genotypes from 70 white-beaked (lagenorhynchus albirostris) and 43 atlantic white-sided dolphins (l. acutus) gathered throughout their north-east atlantic distribution range. both species are at a high risk of being negatively affected by cli ...201625950249
mycotic dermatitis in an atlantic white-sided dolphin, a pygmy sperm whale, and two harbor seals.an atlantic white-sided dolphin (lagenorhynchus acutus), a pygmy sperm whale (kogia breviceps), 2 harbor seals (phoca vitulina) developed raised, firm, erythematous, cutaneous nodules that were most prominent on their heads, trunks, and on the caudal portions of their bodies. prior to the onset of the condition, all 4 animals may have been stressed by factors such as being stranded on a beach, being transported long distances, or being relocated locally. microbial culturing of the lesions on mul ...19968617632
insights into genetic diversity, parentage, and group composition of atlantic white-sided dolphins (lagenorhynchus acutus) off the west of ireland based on nuclear and mitochondrial genetic markers.the analysis of stranding events and the application of molecular markers can be powerful tools to study cryptic biological aspects of delphinid species that occur mainly in open ocean habitat. in the present study, we investigated nuclear and mitochondrial genetic variability of atlantic white-sided dolphins that stranded from 1990 to 2006 (n = 42) along the west coast of ireland, using 8 microsatellite loci and 599 bp of the mitochondrial dna control region. results from both classes of marker ...201021059883
catalytic and immunochemical detection of hepatic and extrahepatic microsomal cytochrome p450 1a1 (cyp1a1) in white-sided dolphin (lagenorhynchus acutus).we have characterized microsomal systems and measured the levels of microsomal cytochrome p450 1a1 (cyp1a1) and ethoxyresorufin-o-deethylase (erod) activity in multiple internal organs of male and female white-sided dolphin (lagenorhynchus acutus) from the northwest atlantic ocean. internal organs were sampled within 24h of death, sometimes in a period of hours, collection times which are significantly less than usually seen for marine mammals. tissue autolysis, as assessed by histological analy ...201020005581
hexabromocyclododecane in white-sided dolphins: temporal trend and stereoisomer distribution in tissues.hexabromocyclododecane (hbcd) is a brominated flame retardant used primarily in expanded polystyrene foams and other styrene resins. samples of blubber (n = 57) and liver (n = 16) from atlantic white-sided dolphins, lagenorhynchus acutus, that stranded on the eastern coast of united states between 1993 and 2004 were obtained from the national marine mammal tissue bank (nmmtb). blubber samples from most of these animals (n = 47) were previously analyzed for polybrominated diphenyl ethers (pbde), ...200818505011
volumetric neuroimaging of the atlantic white-sided dolphin (lagenorhynchus acutus) brain from in situ magnetic resonance images.the structure and development of the brain are extremely difficult to study in free-ranging marine mammals. here, we report measurements of total white matter (wm), total gray matter (gm), cerebellum (wm and gm), hippocampus, and corpus callosum made from magnetic resonance (mr) images of fresh, postmortem brains of the atlantic white-sided dolphin (lagenorhynchus acutus) imaged in situ (i.e., the brain intact within the skull, with the head still attached to the body). wm:gm volume ratios of th ...200818286607
neuroanatomy of the subadult and fetal brain of the atlantic white-sided dolphin (lagenorhynchus acutus) from in situ magnetic resonance images.this article provides the first anatomically labeled, magnetic resonance imaging (mri) -based atlas of the subadult and fetal atlantic white-sided dolphin (lagenorhynchus acutus) brain. it differs from previous mri-based atlases of cetaceans in that it was created from images of fresh, postmortem brains in situ rather than extracted, formalin-fixed brains. the in situ images displayed the classic hallmarks of odontocete brains: fore-shortened orbital lobes and pronounced temporal width. olfactor ...200717957751
factors influencing persistent organic pollutant concentrations in the atlantic white-sided dolphin (lagenorhynchus acutus).assessing the trends of persistent organic pollutants (pops) in cetaceans is difficult because of age and gender influences on accumulation. persistent organic pollutants bioaccumulate and are poorly metabolized; hence, concentrations may increase with age in males while females reduce their pop burden through parturition and lactation. age and gender effects on contaminant concentrations are species specific because of life history and reproductive strategies. these influences must be understoo ...200516110985
persistent organic pollutants in two dolphin species with focus on toxaphene and polybrominated diphenyl ethers.assessing trends of persistent organic pollutants (pops) in marine mammals is difficult due to age, gender, and metabolism influences on accumulation. to help elucidate these effects in dolphins, pop concentrations were determined in the atlantic white-sided dolphin, lagenorhynchus acutus, a pelagic delphinid inhabiting north atlantic waters, and in the rough-toothed dolphin, steno bredanensis, a pelagic delphinid inhabiting tropical and subtropical waters. the specific objectives of this study ...200515757328
white-sided dolphin metallothioneins: purification, characterisation and potential role.metallothioneins (mts) were characterised in the kidneys of a white-sided dolphin lagenorhynchus acutus stranded along the belgian coast, displaying high levels of cadmium (cd) and mercury (hg) in liver and kidney. the protein has two isoforms: mt-1 and mt-2. mt-1 binds cu, zn, hg and cd, while mt-2 only binds zn, hg and cd. this suggests different metabolic functions for the two isoforms: mt-1 is mainly involved in cu homeostasis; mt-2, which was four-fold more abundant than mt-1, detoxifies mo ...200211912049
cadmium-containing granules in kidney tissue of the atlantic white-sided dolphin (lagenorhyncus acutus) off the faroe islands.top predators from the northern sub-polar and polar areas exhibit high cadmium concentrations in their tissues. in the aim to reveal possible adverse effects, samples of five atlantic white-sided dolphins lagenorhyncus acutus have been collected on the occasion of the drive fishery in the faroe islands, for ultrastructural investigations and energy dispersive x-ray microanalyses. cadmium concentrations were less than the limit of detection in both immature individuals and ranged from 22.7 to 31. ...200111701395
concentrations and patterns of organic contaminants in atlantic white-sided dolphins (lagenorhynchus acutus) from irish and scottish coastal waters.chlorobiphenyls (cbs) and organochlorine pesticides (ocps) have been determined in the blubber of 17 atlantic white-sided dolphins stranded at killala bay, co mayo, ireland and five from the scottish coast. the concentrations of the contaminants measured range from 773 to 63,400 microg kg(-1) for sigmacb and from 160 to 54,600 microg kg(-1) for sigmaddt. the concentrations of the cbs and ocps is highly dependent on the age, sex, reproductive state and nutritional condition of the animals in addi ...199715093341
polybrominated diphenyl ethers (pbdes) in marine mammals from arctic and north atlantic regions, 1986-2009.a selection of pbde congeners was analyzed in pooled blubber samples of pilot whale (globicephala melas), ringed seal (phoca hispida), minke whale (balaenoptera acutorostrata), fin whale (balaenoptera physalus), harbor porpoise (phocoena phocoena), hooded seal (cystophora cristata) and atlantic white-sided dolphin (lagenorhynchus acutus), covering a time period of more than 20 years (1986-2009). the analytes were extracted and cleaned-up using open column extraction and multi-layer silica gel co ...201221802148
comparative anatomy of the foramen ovale in the hearts of cetaceans.the structure of the cardiac foramen ovale from 17 species representing six cetacean families, the monodontidae, phocoenidae, delphinidae, ziphiidae, balaenidae and the balaenopteridae, was studied using the scanning electron microscope. eight white whale fetuses (delphinapterus leucas) and a narwhal fetus (monodon monoceros) represented the monodontidae; one fetal and nine neonatal harbour porpoises (phocoena phocoena) and a finless porpoise fetus (neophocoena phocoenoides) represented the phoc ...200717532800
dolphin pox: a skin disease of cetaceans.poxvirus has been identified morphologically from skin lesions in captive and free-ranging bottlenosed dolphins, tursiops truncatus and a stranded atlantic white-sided dolphin, lagenorhynchus acutus. the lesions, commonly referred to as ring or pinhole lesions, appear as solitary or coalesced circular grey blemishes. advanced ring lesions may take the form of black punctiform stippled patterns known as "tattoo". histologically, the stratum externum is thickened, and there is ballooning degenerat ...1976232852
trace element concentrations in cetacean liver tissues archived in the national marine mammal tissue bank.the national biomonitoring specimen bank (nbsb), a collaborative project of several u.s. government agencies, includes marine mammal tissues collected for the alaska marine mammal tissue archival project (ammtap) and the national marine mammal tissue bank (nmmtb). tissues were collected from 139 animals representing 13 species of marine mammals from around the u.s. recently, concentrations for up to 30 elements in liver tissues of nine long-finned pilot whales (globicephala melas), six harbor po ...19958560237
intramuscular sarcocystis in two beluga whales and an atlantic white-sided dolphin from the st. lawrence estuary, quebec, canada. 19938507713
passive cambering and flexible propulsors: cetacean flukes.the flukes are the primary locomotor structure in cetaceans, which produce hydrodynamic thrust as the caudal vertebrae are oscillated dorso-ventrally. effective thrust generation is a function of the kinematics of the flukes, the angle of attack between the flukes and the incident water flow, and the shape of the flukes. we investigated the effect of bending within the caudal region of odontocete cetaceans to determine how changes in angular displacement between caudal vertebrae could effect pas ...200617671317
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