molecular identification of ehrlichia species and 'candidatus neoehrlichia mikurensis' from ticks and wild rodents in shizuoka and nagano prefectures, japan.a total of 293 ticks and 111 wild rodents that were collected in shizuoka and nagano prefectures, japan, were examined for infection of ehrlichia species and 'candidatus neoehrlichia mikurensis.' the 16s rdna or the omp-1 gene of these bacterial dnas were detected from the spleens of tick-inoculated mice (5 positive/total 29 mice) or from the spleens of wild rodents (25 positive/total 111 rodents) by pcr amplifi-cation. sequencing of the 16s rdna revealed ehrlichia spp. from the 5 tick-inoculate ...200616428872
new findings on members of the family anaplasmataceae of veterinary importance.members of the family anaplasmataceae are obligate intracellular gram-negative bacteria that naturally infect a variety of wild and domestic animal species, the spillover of which may lead to zoonosis. i discuss new findings on members of the family anaplasmataceae of veterinary importance and therefore, i will describe the recent findings on neorickettsia risticii in the trematode and related neorickettsia species. i also will review the recent progress on aegyptianella pullorum and other aegyp ...200617114752
molecular survey of babesia microti, ehrlichia species and candidatus neoehrlichia mikurensis in wild rodents from shimane prefecture, japan.a significant number of patients are diagnosed with "fevers of unknown origin" (fuo) in shimane prefecture in japan where tick-borne diseases are endemic. we conducted molecular surveys for babesia microti, ehrlichia species, and candidatus neoehrlichia mikurensis in 62 fuo cases and 62 wild rodents from shimane prefecture, japan. pcr using primers specific for the babesia 18s small-subunit rrna (rdna) gene and anaplasmataceae groesl amplified products from 45% (28/62) and 25.8% (16/62) of captu ...200717446675
diversity of ixodes ricinus tick-associated bacterial communities from different forests.nymphal ixodes ricinus ticks (n=180) were collected from three different areas in the netherlands to investigate the effect of forest composition on tick-associated microbial communities. sampled habitats differed in thickness of leaf litter and humus layers and vegetation associations and were located near amsterdam (beech-oak), ede (birch-oak) and veldhoven (birch-oak). analysis of nine 16s rrna gene clone libraries made from individual ticks showed nearest matches with presumed pathogens cand ...200818355299
raccoons (procyon lotor), but not rodents, are natural and experimental hosts for an ehrlichial organism related to "candidatus neoehrlichia mikurensis"."candidatus neoehrlichia mikurensis" has been reported from a variety of rodent and ixodes tick species in europe and asia. recently, an ehrlichial organism closely related to "candidatus neoehrlichia mikurensis" was cultured from a raccoon (procyon lotor) from georgia, usa. to determine prevalence and distribution, we conducted a molecular survey of free-ranging raccoons (n=197) from 10 populations in 3 states and found that infections were common in tick-infested populations (50-94%). in an ef ...200818524503
incidence of various tick-borne microorganisms in rodents and ticks of central this study, we detected rickettsia helvetica, candidatus midichloria mitochondrii, anaplasma phagocytophilum, ehrlichia muris, candidatus neoehrlichia mikurensis, and bartonella sp. infections in wild rodents and ticks collected from the vegetation of central slovakia. the microorganisms were identified by pcr and sequencing. yellow-necked mice (apodemus flavicollis) were infected with e. muris and bartonella sp., while ticks ixodes ricinus collected from the vegetation were infected with r. ...200818999892
first case of human "candidatus neoehrlichia mikurensis" infection in a febrile patient with chronic lymphocytic immunocompromised patient presented with febrile episodes, an erysipelas-like rash, and thromboembolic complications. amplification of 16s rrna gene sequences from blood and sequence analysis revealed "candidatus neoehrlichia mikurensis." we report the first case of human disease caused by "ca. neoehrlichia mikurensis."201020220155
detection of "candidatus neoehrlichia mikurensis" in two patients with severe febrile illnesses: evidence for a european sequence variant.recently, a new genus of anaplasmataceae termed "candidatus neoehrlichia" was discovered in ticks and rodents. here, we report on two patients who suffered from febrile bacteremia due to "candidatus neoehrlichia mikurensis" associated with thrombotic or hemorrhagic events. 16s rrna and groel gene sequencing provided evidence of three groups of sequence variants.201020519481
septicemia caused by tick-borne bacterial pathogen candidatus neoehrlichia mikurensis.we have repeatedly detected candidatus neoehrlichia mikurensis, a bacterium first described in rattus norvegicus rats and ixodes ovatus ticks in japan in 2004 in the blood of a 61-year-old man with signs of septicemia by 16s rrna and groel gene pcr. after 6 weeks of therapy with doxycycline and rifampin, the patient recovered.201020587186
candidatus neoehrlichia mikurensis infection identified in 2 hematooncologic patients: benefit of molecular techniques for rare pathogen detection.hematooncologic patients often host rare or fastidious pathogens. using 16s rdna sequencing and transmission electron microscopy, we have identified 2 lymphoma patients infected with candidatus neoehrlichia mikurensis. in both individuals, the clinical presentation suggested ehrlichiosis-like syndrome. we believe that molecular techniques open new vistas in the field of pathogen detection.201121353949
"candidatus neoehrlichia mikurensis" infection in a dog from germany."candidatus neoehrlichia mikurensis" is a new intracellular pathogen associated with human infection and death. "candidatus neoehrlichia mikurensis" infection in a chronically neutropenic dog from germany was confirmed by dna sequencing. the same organism was previously described from ticks and two sick human beings from germany.201121367991
wild rodents and novel human pathogen candidatus neoehrlichia mikurensis, southern sweden.we examined small mammals as hosts for anaplasmataceae in southern sweden. of 771 rodents, 68 (8.8%) were infected by candidatus neoehrlichia mikurensis, but no other anaplasmataceae were found. candidatus n. mikurensis has recently been found in human patients in germany, switzerland, and sweden, which suggests that this could be an emerging pathogen in europe.201121888802
"Candidatus Neoehrlichia mikurensis", Anaplasma phagocytophilum and Lyme disease spirochetes in questing European vector ticks and in feeding ticks removed from people.To estimate the likelihood of people coming into contact with the recently described tick-borne agent "Candidatus Neoehrlichia mikurensis", we compared its frequencies with those of Lyme disease spirochetes and Anaplasma phagocytophilum in questing adult Ixodes ricinus ticks collected in various Central European sites and examined ticks, which had been removed from people, for the presence of these pathogens. Whereas spirochetes infected questing adult ticks most frequently (22.3%), more than a ...201122205824
[study of the heterogeneity of 16s rrna gene and groesl operone in the dna samples of anaplasma phagocytophilum, ehrlichia muris, and "candidatus neoehrlichia mikurensis" determined in the ixodes persulcatus ticks in the area of urals, siberia, and far east of russia].a total of 3552 ixodes persulcatus from sverdlovsk, chelyabinsk, novosibirsk, irkutsk regions and khabarovsk territory were examined on the ehrlichia and anaplasma presence by nested pcr based on the 16s rrna gene. both anaplasma phagocytophilum and ehrlichia muris dna were found in i. persulcatus in all studied regions. a. phagocytophilum was detected in 1.3-6.3% of ticks and e. muris - in 2.0-14.1% of ticks. moreover, "candidatus neoehrlichia mikurensis" dna was found in 8 ticks collected in n ...201121786632
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