| cloning and nucleotide sequencing of the membrane-bound l-sorbosone dehydrogenase gene of acetobacter liquefaciens ifo 12258 and its expression in gluconobacter oxydans. | cloning and expression of the gene encoding acetobacter liquefaciens ifo 12258 membrane-bound l-sorbosone dehydrogenase (sndh) were studied. a genomic library of a. liquefaciens ifo 12258 was constructed with the mobilizable cosmid vector pvk102 (mob+) in escherichia coli s17-1 (tra+). the library was transferred by conjugal mating into gluconobacter oxydans ox4, a mutant of g. oxydans ifo 3293 that accumulates l-sorbosone in the presence of l-sorbose. the transconjugants were screened for sndh ... | 1995 | 7574579 |
| the phylogeny of acetic acid bacteria based on the partial sequences of 16s ribosomal rna: the elevation of the subgenus gluconoacetobacter to the generic level. | thirty-six strains of acetic acid bacteria classified in the genera acetobacter, gluconobacter, and acidomonas were examined for their partial base sequences in positions 1220 through 1375, 156 bases, of 16s rrna. the strains of the q10-equipped gluconobacter species examined were divided into two subgroups, which included the type strains of gluconobacter oxydans, the type species of the genus gluconobacter, and of a second species, gluconobacter cerinus, respectively. the base differences numb ... | 1997 | 9301103 |
| decolourization and biodegradation of n,n'-dimethyl-p-phenylenediamine by klebsiella pneumoniae rs-13 and acetobacter liquefaciens s-1. | klebsiella pneumoniae rs-13 and acetobacter liquefaciens s-1, both methyl red (mr)-degrading bacterial strains, degraded n,n'-dimethyl-p-phenylenediamine (dmpd) under aerobic conditions. dmpd, a toxic and mutagenic aromatic amine, is formed during the reductive cleavage of azo dyes such as mr. the effects of physical parameters, such as temperature and aeration, and chemical parameters, such as ph and concentrations of glucose, ethanol and ammonium sulphate in the culture medium, on the degradat ... | 1998 | 9721658 |
| description of gluconacetobacter sacchari sp. nov., a new species of acetic acid bacterium isolated from the leaf sheath of sugar cane and from the pink sugar-cane mealy bug. | a new species of the genus gluconacetobacter, for which the name gluconacetobacter sacchari sp. nov. is proposed, was isolated from the leaf sheath of sugar cane and from the pink sugar-cane mealy bug, saccharicoccus sacchari, found on sugar cane growing in queensland and northern new south wales, australia. the nearest phylogenetic relatives in the alpha-subclass of the proteobacteria are gluconacetobacter liquefaciens and gluconacetobacter diazotrophicus, which have 98.8-99.3% and 97.9-98.5% 1 ... | 1999 | 10555349 |
| molecular detection of gluconacetobacter sacchari associated with the pink sugarcane mealybug saccharicoccus sacchari (cockerell) and the sugarcane leaf sheath microenvironment by fish and pcr. | molecular tools for the detection of the newly described acetic acid bacterium gluconacetobacter sacchari from the pink sugarcane mealybug, saccharicoccus sacchari cockerell (homiptera: pseudococcidae), and in the sugarcane leaf sheath microenvironment were developed. g. sacchari specific 16s rrna-targeted oligonucleotide primers were designed and used in pcr amplification of g. sacchari dna directly from mealybugs, and in a nested pcr to detect low numbers of the bacteria from sugarcane leaf sh ... | 2000 | 10620720 |
| transfer of acetobacter oboediens sokollek et al 1998 and acetobacter intermedius boesch et al. 1998 to the genus gluconacetobacter as gluconacetobacter oboediens comb. nov. and gluconacetobacter intermedius comb. nov. | acetobacter oboediens sokollek et al. 1998 and acetobacter intermedius boesch et al. 1998 are transferred to the genus gluconacetobacter as gluconacetobacter oboediens comb. nov. and gluconacetobacter intermedius comb. nov. because, on the basis of their 16s rrna gene sequences, the type strains of both species are located in the cluster of the genus gluconacetobacter along with those of gluconacetobacter xylinus, gluconacetobacter europaeus, gluconacetobacter hansenii, gluconacetobacter liquefa ... | 2000 | 11155999 |
| novel nitrogen-fixing acetic acid bacteria, gluconacetobacter johannae sp. nov. and gluconacetobacter azotocaptans sp. nov., associated with coffee plants. | diazotrophic bacteria were isolated, in two different years, from the rhizosphere and rhizoplane of coffee (coffea arabica l.) plants cultivated in mexico; they were designated as type dor and type sad isolates. they showed characteristics of the family acetobacteraceae, having some features in common with gluconacetobacter (formerly acetobacter) diazotrophicus, the only known n2-fixing species of the acetic acid bacteria, but they differed from this species with regard to several characteristic ... | 2001 | 11491326 |
| cloning of escherichia coli lacz and lacy genes and their expression in gluconobacter oxydans and acetobacter liquefaciens. | an efficient transformation protocol for gluconobacter oxydans and acetobacter liquefaciens strains was developed by preparation of electrocompetent cells grown on yeast extract-ethanol medium. plasmid pbbr122 was used as broad-host-range vector to clone the escherichia coli laczy genes in g. oxydans and a. liquefaciens. although both lac genes were functionally expressed in both acetic acid bacteria, only a few transformants were able to grow on lactose. however, this ability strictly depended ... | 2002 | 11976147 |
| kozakia baliensis gen. nov., sp. nov., a novel acetic acid bacterium in the alpha-proteobacteria. | four bacterial strains were isolated from palm brown sugar and ragi collected in bali and yogyakarta, indonesia, by an enrichment culture approach for acetic acid bacteria. phylogenetic analysis based on 16s rrna gene sequences showed that the four isolates constituted a cluster separate from the genera acetobacter, gluconobacter, acidomonas, gluconacetobacter and asaia with a high bootstrap value in a phylogenetic tree. the isolates had high values of dna-dna similarity (78-100%) between one an ... | 2002 | 12054243 |
| differentiation of gluconacetobacter liquefaciens and gluconacetobacter xylinus on the basis of dna base composition, dna relatedness, and oxidation products from glucose. | gluconacetobacter liquefaciens and gluconacetobacter xylinus share very similar phenotypic characteristics. they are differentiated by the production of a reddish-brown water-soluble pigment of the former and cellulose production of the latter. however, the loss of the two distinguishing features questions the separate standings of the two species. the dna base composition and the dna relatedness of strains of the two species, including other established species of acetic acid bacteria, were det ... | 1999 | 12501396 |
| design, development, and use of molecular primers and probes for the detection of gluconacetobacter species in the pink sugarcane mealybug. | molecular tools for the species-specific detection of gluconacetobacter sacchari, gluconacetobacter diazotrophicus, and gluconacetobacter liquefaciens from the pink sugarcane mealybug (psmb) saccharicoccus sacchari cockerell (homiptera: pseudococcidae) were developed and used in polymerase chain reactions (pcr) and in fluorescence in situ hybridizations (fish) to better understand the microbial diversity and the numerical significance of the acetic acid bacteria in the psmb microenvironment. the ... | 2005 | 16047097 |
| antagonism among gluconacetobacter diazotrophicus strains in culture media and in endophytic association. | in this study the antagonistic activity among 55 gluconacetobacter diazotrophicus strains, belonging to 13 electrophoretic types (ets), in culture media was analyzed. antagonistic effects were seen only in strains belonging to two ets named et-1 and et-3. two out of 29 et-1 strains, and 3 out of 7 et-3 strains of g. diazotrophicus showed antagonistic effects against many other strains belonging to all the ets of this species analyzed, and against closely related strains of gluconacetobacter spec ... | 2005 | 16329972 |
| cloning and nucleotide sequencing of the membrane-bound l-sorbosone dehydrogenase gene of acetobacter liquefaciens ifo 12258 and its expression in gluconobacter oxydans. | volume 61, no. 2, p. 419, column 1, lines 15-19: this sentence should read as follows. "the alcohol dehydrogenase and glucose dehydrogenase have a common region reported to be related to pyrroloquinoline quinone binding (2, 10), but sndh does not contain such a region, indicating that sndh is not a quinoprotein." page 419, column 2, line 12: "(table 4)" should read "(table 3)." [this corrects the article on p. 413 in vol. 61.]. | 1995 | 16535037 |
| validation of the (gtg)(5)-rep-pcr fingerprinting technique for rapid classification and identification of acetic acid bacteria, with a focus on isolates from ghanaian fermented cocoa beans. | amplification of repetitive bacterial dna elements through the polymerase chain reaction (rep-pcr fingerprinting) using the (gtg)(5) primer, referred to as (gtg)(5)-pcr fingerprinting, was found a promising genotypic tool for rapid and reliable speciation of acetic acid bacteria (aab). the method was evaluated with 64 aab reference strains, including 31 type strains, and 132 isolates from ghanaian, fermented cocoa beans, and was validated with dna:dna hybridization data. most reference strains, ... | 2008 | 17920717 |
| genera and species in acetic acid bacteria. | taxonomic studies of acetic acid bacteria were historically surveyed. the genus acetobacter was first introduced in 1898 with a single species, acetobacter aceti. the genus gluconobacter was proposed in 1935 for strains with intense oxidation of glucose to gluconic acid rather than oxidation of ethanol to acetic acid and no oxidation of acetate. the genus "acetomonas" was described in 1954 for strains with polar flagellation and no oxidation of acetate. the proposals of the two generic names wer ... | 2008 | 18199517 |
| tanticharoenia sakaeratensis gen. nov., sp. nov., a new osmotolerant acetic acid bacterium in the alpha-proteobacteria. | tanticharoenia sakaeratensis gen. nov., sp. nov. is proposed for three strains isolated from soil collected in thailand. the three strains, ac37(t), ac38, and ac39, were included within a lineage comprising the genera asaia, kozakia, swaminathania, neoasaia, acetobacter, gluconobacter, and saccharibacter in a phylogenetic tree based on 16s rrna gene sequences, but formed a quite different, independent cluster. pair-wise sequence similarities of strain ac37(t) were 96.5-92.1% to the type strains ... | 2008 | 18323667 |
| cold atmospheric plasma decontamination of the pericarps of fruit. | this investigation describes the inactivation by cold atmospheric plasmas of one pathogenic and three spoilage organisms on the pericarps of mangoes and melons. the operating voltage necessary for efficient microbial decontamination of fruit pericarps was first established using escherichia coli at a concentration of 10(7) cfu/cm2 on the surface of mango. it was found that, when the plasma was sustained slightly above its breakdown voltage of 12 kv (peak to peak), no inactivation was detected wh ... | 2008 | 18326179 |
| ameyamaea chiangmaiensis gen. nov., sp. nov., an acetic acid bacterium in the alpha-proteobacteria. | two isolates, ac04(t) and ac05, were isolated from the flowers of red ginger collected in chiang mai, thailand. in phylogenetic trees based on 16s rrna gene sequences, the two isolates were included within a lineage comprised of the genera acidomonas, gluconacetobacter, asaia, kozakia, swaminathania, neoasaia, granulibacter, and tanticharoenia, and they formed an independent cluster along with the type strain of tanticharoenia sakaeratensis. the calculated pair-wise sequence similarities of isol ... | 2009 | 19809199 |
| screening for and isolation and identification of malathion-degrading bacteria: cloning and sequencing a gene that potentially encodes the malathion-degrading enzyme, carboxylestrase in soil bacteria. | five malathion-degrading bacterial strains were enriched and isolated from soil samples collected from different agricultural sites in cairo, egypt. malathion was used as a sole source of carbon (50 mg/l) to enumerate malathion degraders, which were designated as is1, is2, is3, is4, and is5. they were identified, based on their morphological and biochemical characteristics, as pseudomonas sp., pseudomonas putida, micrococcus lylae, pseudomonas aureofaciens, and acetobacter liquefaciens, respecti ... | 2010 | 20401686 |
| production of 4-keto-d-arabonate by oxidative fermentation with newly isolated gluconacetobacter liquefaciens. | production of 4-keto-d-arabonate (4kab) was confirmed in a culture medium of gluconacetobacter liquefaciens strains, newly isolated from water kefir in argentina. the strains rapidly oxidized d-glucose, d-gluconate (ga), and 2-keto-d-gluconate (2kga), and accumulated 2,5-diketo-d-gluconate (25dka) exclusively before reaching the stationary phase. 25dka was in turn converted to 4kab, and 4kab remained stable in the culture medium. the occurrence of 4kab was assumed by ameyama and kondo about 50 y ... | 2010 | 21150092 |
| formation of 4-keto-d-aldopentoses and 4-pentulosonates (4-keto-d-pentonates) with unidentified membrane-bound enzymes from acetic acid bacteria. | in our previous study, a new microbial reaction yielding 4-keto-d-arabonate from 2,5-diketo-d-gluconate was identified with gluconacetobacter liquefaciens rctmr 10. it appeared that decarboxylation and dehydrogenation took place together in the reaction. to analyze the nature of the reaction, investigations were done with the membrane fraction of the organism, and 4-keto-d-arabinose was confirmed as the direct precursor of 4-keto-d-arabonate. two novel membrane-bound enzymes, 2,5-diketo-d-glucon ... | 2011 | 21897028 |
| numerical analysis of phenotypic features and protein gel electrophoregrams of a wide variety of acetobacter strains. proposal for the improvement of the taxonomy of the genus acetobacter beijerinck 1898, 215. | ninety-eight strains, representing all acetobacter species and subspecies from the approved lists of bacterial names (skerman et al., 1980), were examined in a numerical analysis of 177 phenotypic features and compared to ninety-eight gluconobacter and seven frateuria strains. four phenons could be delineated, corresponding to frateuria (phenon 1), a. aceti subsp. liquefaciens (phenon 2), gluconobacter (phenon 3) and acetobacter minus a. aceti subsp. liquefaciens (phenon 4). acetobacter, frateur ... | 1983 | 23194734 |
| endobacter medicaginis gen. nov., sp. nov., isolated from alfalfa nodules in an acidic soil. | a bacterial strain designated m1ms02(t) was isolated from a surface-sterilized nodule of medicago sativa in zamora (spain). the 16s rrna gene sequence of this strain showed 96.5 and 96.2 % similarity, respectively, with respect to gluconacetobacter liquefaciens ifo 12388(t) and granulibacter bethesdensis cgdnih1(t) from the family acetobacteraceae. the novel isolate was a gram-stain-negative, non-sporulating, aerobic coccoid to rod-shaped bacterium that was motile by a subpolar flagellum. the ma ... | 2013 | 23002052 |