| assignment of eubacterium sp. vpi 12708 and related strains with high bile acid 7alpha-dehydroxylating activity to clostridium scindens and proposal of clostridium hylemonae sp. nov., isolated from human faeces. | unknown eubacterium-like organisms vpi 12708 and five strains (y-1113, i-10, m-18, th-82 and 36s) had high bile acid 7alpha-dehydroxylating activity; the unknown clostridium-like organisms tn-271t and tn-272 also had the same activity. analysis of their 16s rdna sequences demonstrated that all strains belong to cluster xiva of the genus clostridium (collins et al., 1994). strain vpi 12708 and five other strains (y-1113, i-10, m-18, th-82 and 36s) formed a single cluster and strains tn-271t and t ... | 2000 | 10843034 |
| deoxycholic acid formation in gnotobiotic mice associated with human intestinal bacteria. | in humans and animals, intestinal flora is indispensable for bile acid transformation. the goal of our study was to establish gnotobiotic mice with intestinal bacteria of human origin in order to examine the role of intestinal bacteria in the transformation of bile acids in vivo using the technique of gnotobiology. eight strains of bile acid-deconjugating bacteria were isolated from ex-germ-free mice inoculated with a human fecal dilution of 10(-6), and five strains of 7alpha-dehydroxylating bac ... | 2006 | 17152920 |
| isolation and characterization of a bile acid inducible 7alpha-dehydroxylating operon in clostridium hylemonae tn271. | primary bile acids are synthesized from cholesterol in the liver, conjugated to either glycine or taurine and secreted into bile. bile salts undergo enterohepatic circulation several times each day. during this process, they are biotransformed into a variety of metabolites by gut bacteria. the major biotransformation is the 7alpha-dehydroxylation of cholic acid and chenodeoxycholic acid yielding deoxycholic acid and lithocholic acid, respectively. 7alpha-dehydroxylation is a multi-step pathway. ... | 2010 | 19464381 |