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selection and validation of reference genes for real-time rt-pcr studies in the non-model species delomys sublineatus, an endemic brazilian rodent.quantitative real-time rt-pcr (qrt-pcr) is a sensitive technique for gene expression analysis. a critical factor for creating reliable data in relative quantification is the normalization of the expression data of genes of interest. therefore the needed normalization factor is calculated out of the expression data of co-amplified genes that are stable expressed in the certain sample material, the so-called reference genes. in this study, we demonstrate the important process of validating potenti ...201020059981
are associations of immune gene expression, body condition and parasite burden detectable in nature? a case study in an endemic rodent from the brazilian atlantic forest.host-parasite co-evolutionary processes are the most important drivers shaping the host's immune system. during successful host immune responses to helminthic infections, usually a balanced cascade of different immune genes like mhc, t helper cell 1 and 2 (th1 and th2) cytokines is expressed. this information comes largely from human or laboratory studies. the situation under which the immune system has evolved, however, is more complicated and natural variation need to be included to provide a ...201021074637
permanent genetic resources added to molecular ecology resources database 1 april 2010 - 31 may 2010.this article documents the addition of 396 microsatellite marker loci to the molecular ecology resources database. loci were developed for the following species: anthocidaris crassispina, aphis glycines, argyrosomus regius, astrocaryum sciophilum, dasypus novemcinctus, delomys sublineatus, dermatemys mawii, fundulus heteroclitus, homalaspis plana, jumellea rossii, khaya senegalensis, mugil cephalus, neoceratitis cyanescens, phalacrocorax aristotelis, phytophthora infestans, piper cordulatum, pte ...201021565124
an integrative appraisal of the diversification in the atlantic forest genus delomys (rodentia: cricetidae: sigmodontinae) with the description of a new species.recent taxonomic studies on neotropical mammals have benefited from the use of genetic data to unravel and recognize species diversity in a number of genera, including the atlantic forest endemic genus delomys. however, the success of this approach depends on ability to link genetically identified lineages to species names based on voucher specimens that lack genetic data. cytogenetic studies in the atlantic forest endemic rodent genus delomys have revealed two widespread karyotypes, 2n=72/fn=90 ...201424870069
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