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lactobacillus acidipiscis sp. nov. and weissella thailandensis sp. nov., isolated from fermented fish in thailand.eleven strains of homofermentative, rod-shaped lactic acid bacteria and five strains of heterofermentative, sphere-shaped lactic acid bacteria were isolated from fermented fish (pla-ra and pla-chom) in thailand. they were identified as new species and named lactobacillus acidipiscis sp. nov. and weissella thailandensis sp. nov., respectively, on the basis of phylogenetic analysis of the 16s rrna gene sequences, dna relatedness and phenotypic characteristics. the type strain of l. acidipiscis is ...200010939653
lactobacillus cypricasei sp. nov., isolated from halloumi cheese.four strains of a hitherto unknown bacterium isolated from halloumi cheese were compared by using phenotypic and phylogenetic studies. comparative 16s rrna gene sequencing demonstrated that the strains were identical to each other and represent a new subline within the genus lactobacillus. the unknown bacterium was readily distinguished from other described gram-positive catalase-negative taxa by means of biochemical tests and electrophoretic analysis of whole-cell proteins. on the basis of phyl ...200111211271
lactic acid bacteria isolated from soy sauce mash in thailand.fourteen sphere-shaped and 30 rod-shaped lactic acid bacteria were isolated from soy sauce mash of two factories in thailand. these strains were separated into two groups, group a and group b, by cell shape and dna-dna similarity. group a contained 14 tetrad-forming strains, and these strains were identified as tetragenococcus halophilus by dna similarity. group b contained 30 rod-shaped bacteria, and they were further divided into four subgroups, b1, b2, b3, and b4, and three ungrouped strains ...200212469319
lactobacillus cypricasei lawson et al. 2001 is a later heterotypic synonym of lactobacillus acidipiscis tanasupawat et al. 2000.the applicability of a multilocus sequence analysis (mlsa)-based identification system for lactobacilli was evaluated. two housekeeping genes that code for the phenylalanyl-trna synthase alpha-subunit (phes) and rna polymerase alpha-subunit (rpoa) were sequenced and analysed for members of the lactobacillus salivarius species group. the type strains of lactobacillus acidipiscis and lactobacillus cypricasei were investigated further using a third gene that encodes the alpha-subunit of atp synthas ...200616825650
lactobacillus hayakitensis sp. nov., isolated from intestines of healthy thoroughbreds.two strains, kbl13(t) and gbl13, were isolated as one of intestinal lactobacilli from the faecal specimens from different thoroughbreds of the same farm where they were born in hokkaido, japan. they were gram-positive, facultatively anaerobic, catalase-negative, non-spore-forming and non-motile rods. kbl13(t) and gbl13 homofermentatively metabolize glucose, and produce lactate as the sole final product from glucose. the 16s rrna gene sequence, dna-dna hybridization, dna g+c content and biochemic ...200718048734
lactobacillus pobuzihii sp. nov., isolated from pobuzihi (fermented cummingcordia).twenty-one homofermentative lactic acid bacteria were isolated from fermented cummingcordia (pobuzihi), a traditional food in taiwan. the isolates had identical 16s rrna gene sequences that were distinct from those of other lactobacilli, and their closest neighbours in the 16s rrna gene sequence phylogenetic tree were strains of lactobacillus acidipiscis. levels of dna-dna relatedness between representative pobuzihi isolates and strains of l. acidipiscis were 17% and below. furthermore, the new ...201019783610
characterization of plac1, a cryptic plasmid isolated from lactobacillus acidipiscis and comparative analysis with its related plasmids.the plac1 plasmid of lactobacillus acidipiscis aca-dc 1533, a strain isolated from traditional kopanisti cheese, was characterised. nucleotide sequence analysis revealed a circular molecule of 3478bp with a g+c content of 37.2%. ab initio annotation indicated four putative open reading frames (orfs). orf1 and orf4 were found to encode a replication initiation protein (rep) and a mobilization protein (mob), respectively. the deduced products of orf2 and orf3 revealed no significant homology to ot ...201020538362
comparison of pcr-dgge and pcr-sscp analysis for bacterial flora of japanese traditional fermented fish products, aji-narezushi and iwashi-nukazuke.the bacterial flora of two japanese traditional fermented fish products, aji-narezushi (salted and long-fermented horse mackerel (trachurus japonicas) with rice) and iwashi-nukazuke (salted and long-fermented sardine (sardinops melanostica) with rice bran), was analysed using non-culture-based polymerase chain reaction (pcr) denaturing gradient gel electrophoresis (dgge) and culture-based pcr single-strand conformation polymorphism (sscp) methods.201020572057
isolation and partial characterization of halotolerant lactic acid bacteria from two mexican cheeses.isolated strains of halotolerant or halophilic lactic acid bacteria (halab) from cotija and doble crema cheeses were identified and partially characterized by phenotypic and genotypic methods, and their technological abilities were studied in order to test their potential use as dairy starter components. humidity, a(w), ph, and salt concentration of cheeses were determined. genotypic diversity was evaluated by randomly amplified polymorphic dna-polymerase chain reaction. molecular identification ...201121327742
genome sequence of lactobacillus cypricasei kctc 13900.lactobacillus cypricasei kctc 13900 is important in the generation of particular flavors and in other ripening processes associated with specific cheeses. here, we announce the draft genome sequence of lactobacillus cypricasei kctc 13900, isolated from cheeses, and describe major findings from its annotation.201121742864
identification and characterization of lactic acid bacteria isolated from mixed pasture of timothy and orchardgrass, and its badly preserved silage.in order to understand the relationship between lactic acid bacteria (lab) species and silage fermentation, a total of 65 lab strains isolated from mixed pasture of timothy (phleum pratense l.) and orchardgrass (dactylis glomerata l.), and its badly preserved silages were subjected to phenotypic and genetic analysis. according to these analyses, the isolates were divided into 13 groups, including enterococcus gallinarum, lactobacillus acidipiscis, l. coryniformis subsp. coryniformis, l. corynifo ...201122515692
phenotypic and genotypic characterization of some lactic acid bacteria isolated from bee pollen: a preliminary study.in the present work, five hundred and sixty-seven isolates of lactic acid bacteria were recovered from raw bee pollen grains. all isolates were screened for their antagonistic activity against both gram-positive and gram-negative pathogenic bacteria. neutralized supernatants of 54 lactic acid bacteria (lab) cultures from 216 active isolates inhibited the growth of indicator bacteria. they were phenotypically characterized, based on the fermentation of 39 carbohydrates. using the simple matching ...201424936378
analyzing the relation between the microbial diversity of daqu and the turbidity spoilage of traditional chinese vinegar.vinegar is a traditional fermented condiment, and the microbial diversity of daqu makes the quality of vinegar products. recently, turbidity spoilage of vinegar sharply tampered with the quality of vinegar. in this study, the relation between the microbial diversity of daqu and turbidity spoilage of vinegar was analyzed by plating technique, pcr-denaturing gradient gel electrophoresis (dgge), and high-performance liquid chromatography (hplc). the 16s rrna sequencing and dgge analysis indicated t ...201424691870
microbial diversity during fermentation of sweet paste, a chinese traditional seasoning, using pcr-denaturing gradient gel electrophoresis.the aim of this study was to elucidate the changes in the microbial community and biochemical properties of a traditional sweet paste during fermentation. pcr-denaturing gradient gel electrophoresis (dgge) analysis showed that aspergillus oryzae was the predominant species in the koji and the majority of the fungi isolated belonged to 2 zygosaccharomyces species in the mash. the bacterial dgge profiles revealed the presence of bacillus subtilis during fermentation, and lactobacillus acidipiscis, ...201728081358
lead biosorption of probiotic bacteria: effects of the intestinal content from laying hens.this study investigated the effects and the possible mechanisms of intestinal content (ic) from laying hens on in vitro lead (pb(2+)) biosorption of four probiotic bacterial strains (bifidobacterium longum bb79, lactobacillus paracasei kgl6, lactobacillus pentosus ita23, and lactobacillus acidipiscis ita44). the total pb(2+) removal capacity of the four probiotic strains, with and without capsule polysaccharides (cpss), increased in the presence of ic compared to the control (without ic). sem im ...201728390025
complete genome sequence of the dairy isolate lactobacillus acidipiscis aca-dc 1533.lactobacillus acidipiscis is a gram-positive lactic acid bacterium belonging to the lactobacillus salivarius clade. here, we present the first complete genome sequence of l. acidipiscis isolated from traditional greek kopanisti cheese. strain aca-dc 1533 may play a key role in the strong organoleptic characteristics of kopanisti cheese.201728126948
protective potential of lactobacillus species in lead toxicity model in broiler chickens.to alleviate adverse effects of heavy metal toxicity, diverse range of removing methods have been suggested, that is usage of algae, agricultural by-products and microorganisms. here, we investigated lead (pb) biosorption efficacy by two lactic acid bacteria species (labs) in broiler chickens. in an in vitro study, pb was added to culture medium of labs (lactobacillus pentosus ita23 and lactobacillus acidipiscis ita44) in the form of lead acetate. results showed that these labs were able to abso ...201627804905
natural lactic acid bacteria population of tropical grasses and their fermentation factor analysis of silage prepared with cellulase and inoculant.natural lactic acid bacteria (lab) populations in tropical grasses and their fermentation characteristics on silage prepared with cellulase enzyme and lab inoculants were studied. a commercial inoculant lactobacillus plantarum chikuso 1 (ch), a local selected strain lactobacillus casei th14 (th14), and 2 cellulases, acremonium cellulase (ac) and maicelase (mc; meiji seika pharma co. ltd., tokyo, japan), were used as additives to silage preparation with fresh and wilted (6 h) guinea grass and nap ...201627743669
tracing microbiota changes in yamahai-moto, the traditional japanese sake starter.sake is made from steamed rice, malted rice, and water. sake production begins with the preparation of a small-scale starter (moto); the quality of moto significantly influences the flavor and richness of sake. in the traditional starter, yamahai-moto, the growth of naturally occurring lactic acid bacteria represses the putrefactive micro-organisms, whereas in the modern starter, sokujo-moto, this is achieved by adding lactic acid. in this study, the successive change in bacterial flora of yamah ...201626479869
detection and genomic characterization of motility in lactobacillus curvatus: confirmation of motility in a species outside the lactobacillus salivarius clade.lactobacillus is the largest genus within the lactic acid bacteria (lab), with almost 180 species currently identified. motility has been reported for at least 13 lactobacillus species, all belonging to the lactobacillus salivarius clade. motility in lactobacilli is poorly characterized. it probably confers competitive advantages, such as superior nutrient acquisition and niche colonization, but it could also play an important role in innate immune system activation through flagellin–toll-like r ...201525501479
draft genome sequence of lactobacillus pobuzihii e100301t.lactobacillus pobuzihii e100301(t) is a novel lactobacillus species previously isolated from pobuzihi (fermented cummingcordia) in taiwan. phylogenetically, this strain is closest to lactobacillus acidipiscis, but its phenotypic characteristics can be clearly distinguished from those of l. acidipiscis. we present the draft genome sequence of strain l. pobuzihii e100301(t).201323661478
microbiota and metabolome during controlled and spontaneous fermentation of nocellara etnea table olives.this study is aimed to investigate bacterial community and its dynamics during the fermentation of nocellara etnea table olives and to study its effect on metabolome formation. six different combination of bacterial cultures (bc1-bc6) were used as starters for table olive fermentation and one additional process, conducted without addition of any starters, was used as control (c). the processes were conducted in triplicate and, overall, 21 vessels were performed at industrial scale. the fermentat ...201728399996
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