| borrelia spielmanii erythema migrans, hungary. | | 2005 | 16422006 |
| the expanding lyme borrelia complex--clinical significance of genomic species? | ten years after the discovery of spirochaetes as agents of lyme disease in 1982 in the usa, three genomic species had diverged from the phenotypically heterogeneous strains of borrelia burgdorferi isolated in north america and europe: borrelia afzelii, b. burgdorferi sensu stricto (further b. burgdorferi), and borrelia garinii. whereas b. burgdorferi remained the only human pathogen in north america, all three species are aetiological agents of lyme borreliosis in europe. another seven genospeci ... | 2011 | 21414082 |
| molecular detection of borrelia bissettii dna in serum samples from patients in the czech republic with suspected borreliosis. | until recently, three spirochete genospecies were considered to be the causative agents of lyme borreliosis (lb) in europe: borrelia burgdorferi sensu stricto, borrelia afzelii and borrelia garinii. however, the dna of borrelia valaisiana, borrelia lusitaniae, borrelia spielmanii and borrelia bissettii has already been detected in samples of human origin, or the spirochetes were isolated from the patients with symptoms of lb. molecular analysis of 12 selected serum samples collected in the regio ... | 2009 | 19187198 |
| then and now: use of 16s rdna gene sequencing for bacterial identification and discovery of novel bacteria in clinical microbiology laboratories. | in the last decade, as a result of the widespread use of pcr and dna sequencing, 16s rdna sequencing has played a pivotal role in the accurate identification of bacterial isolates and the discovery of novel bacteria in clinical microbiology laboratories. for bacterial identification, 16s rdna sequencing is particularly important in the case of bacteria with unusual phenotypic profiles, rare bacteria, slow-growing bacteria, uncultivable bacteria and culture-negative infections. not only has it pr ... | 2008 | 18828852 |
| identification and characterization of the factor h and fhl-1 binding complement regulator-acquiring surface protein 1 of the lyme disease spirochete borrelia spielmanii sp. nov. | borrelia spielmanii, one of the etiological agents of lyme disease found in europe, evades host complement-mediated killing by recruitment of the immune regulators factor h and fhl-1 from human serum. serum-resistant and intermediate serum-resistant isolates express up to 3 distinct complement regulator-acquiring surface proteins (crasps) that bind factor h and/or fhl-1. the present study describes identification and functional characterization of bscrasp-1 as the dominant factor h and fhl-1 bin ... | 2009 | 18706858 |
| characterization of borrelia burgdorferi sensu lato isolates by pulsed-field gel electrophoresis after mlui restriction of genomic dna. | at least three borrelia species (borrelia afzelii, borrelia garinii and borrelia burgdorferi sensu stricto) cause disease in humans, but borrelia spielmanii, borrelia valaisiana, borrelia lusitaniae and borrelia bissettii have also been reported to be rare or potential causes of human disease in europe. pulsed-field gel electrophoresis after mlui restriction of the genomic dna (mlui large restriction fragment patterns, lrfps) represents one of several approaches that have been used to assess bor ... | 2008 | 18586084 |
| human pathogenic borrelia spielmanii sp. nov. resists complement-mediated killing by direct binding of immune regulators factor h and factor h-like protein 1. | borrelia spielmanii sp. nov. has recently been shown to be a novel human pathogenic genospecies that causes lyme disease in europe. in order to elucidate the immune evasion mechanisms of b. spielmanii, we compared the abilities of isolates obtained from lyme disease patients and tick isolate pc-eq17 to escape from complement-mediated bacteriolysis. using a growth inhibition assay, we show that four b. spielmanii isolates, including pc-eq17, are serum resistant, whereas a single isolate, pmew, wa ... | 2007 | 17635869 |
| epidemiological aspects and molecular characterization of borrelia burgdorferi s.l. from southern germany with special respect to the new species borrelia spielmanii sp. nov. | in 475 borrelia-infected ixodes ricinus (2155 ticks investigated) from southern germany the most common borrelia burgdorferi sensu lato species was b. garinii (34.3%) followed by b. afzelii (25.1%), b. burgdorferi sensu stricto (22.0%), and b. valaisiana (12.7%). b. spielmanii sp. nov. was detected in 5.9% of the 475 infected ticks. hints for a focal distribution were found for b. spielmanii sp. nov. and b. garinii ospa type 4. in 242 patient isolates, dominance (66.9%) of b. afzelii for skin co ... | 2008 | 17616434 |
| lyme borreliosis and borrelia spielmanii. | | 2006 | 16848050 |
| delineation of borrelia burgdorferi sensu lato species by multilocus sequence analysis and confirmation of the delineation of borrelia spielmanii sp. nov. | to evaluate multilocus sequence analysis (mlsa) for taxonomic purposes in the delineation of species within borrelia burgdorferi sensu lato, seven relevant loci of various strains for which extensive dna-dna reassociation data were available were sequenced. mlsa delineation proved to be fully concordant with conventional methods. our analysis confirmed the delineation of a novel species, borrelia spielmanii sp. nov., previously known as 'borrelia spielmani' richter et al. 2004, with strain pc-eq ... | 2006 | 16585709 |
| relationships of a novel lyme disease spirochete, borrelia spielmani sp. nov., with its hosts in central europe. | to determine whether the pathogenic variant of lyme disease spirochetes, isolate a14s, is perpetuated in a particular reservoir-vector relationship, we screened vector ticks in various central european sites for a related spirochete and determined its host association. a14s-like spirochetes infect numerous questing ticks in the petite camargue alsacienne (pc). they frequently infect dormice, but no mice or voles. garden dormice appear to be better reservoir hosts for a14s-like spirochetes than f ... | 2004 | 15528500 |
| imbalanced presence of borrelia burgdorferi s.l. multilocus sequence types in clinical manifestations of lyme borreliosis. | in this study we used typing based on the eight multilocus sequence typing scheme housekeeping genes (mlst) and 5s-23s rdna intergenic spacer (igs) to explore the population structure of borrelia burgdorferi sensu lato isolates from patients with lyme borreliosis (lb) and to test the association between the b. burgdorferi s.l. sequence types (st) and the clinical manifestations they cause in humans. isolates of b. burgdorferi from 183 lb cases across europe, with distinct clinical manifestations ... | 2016 | 27125686 |
| borrelia burgdorferi sensu lato and co-infections with anaplasma phagocytophilum and rickettsia spp. in ixodes ricinus in hamburg, germany. | to obtain initial data on borrelia burgdorferi sensu lato (spirochaetales: spirochaetaceae) in ixodes ricinus (ixodida: ixodidae) ticks in hamburg, germany, 1400 questing ticks were collected by flagging at 10 different public recreation areas in 2011 and analysed using probe-based quantitative real-time polymerase chain reaction. the overall rate of infection with b. burgdorferi s.l. was 34.1%; 30.0% of adults were infected (36.7% of females and 26.0% of males), as were 34.5% of nymphs. signifi ... | 2015 | 26096626 |
| transmission differentials for multiple pathogens as inferred from their prevalence in larva, nymph and adult of ixodes ricinus (acari: ixodidae). | ixodes ricinus serves as vector for a range of microorganisms capable of causing clinical illness in humans. the microorganisms occur in the same vector populations and are generally affected by the same tick-host interactions. still, the instars have different host preferences which should manifest in different transmission patterns for various microorganisms in the tick populations, i.e., most microorganisms increase in prevalence rate from larvae to nymphs because their reservoirs are among s ... | 2017 | 28255923 |
| melting pot of tick-borne zoonoses: the european hedgehog contributes to the maintenance of various tick-borne diseases in natural cycles urban and suburban areas. | european hedgehogs (erinaceus europaeus) are urban dwellers and host both ixodes ricinus and ixodes hexagonus. these ticks transmit several zoonotic pathogens like borrelia burgdorferi (sensu lato), anaplasma phagocytophilum, rickettsia helvetica, borrelia miyamotoi and "candidatus neoehrlichia mikurensis". it is unclear to what extent hedgehogs in (sub) urban areas contribute to the presence of infected ticks in these areas, which subsequently pose a risk for acquiring a tick-borne disease. the ... | 2017 | 28270232 |
| hide and seek: how lyme disease spirochetes overcome complement attack. | overcoming the first line of the innate immune system is a general hallmark of pathogenic microbes to avoid recognition and to enter the human host. in particular, spirochetes belonging to the borrelia burgdorferi sensu lato complex have developed various means to counter the immune response and to successfully survive in diverse host environments for a prolonged period of time. in regard to complement resistance, borrelia utilize a plethora of immune evasion strategies involves capturing of hos ... | 2016 | 27725820 |
| high-throughput screening of tick-borne pathogens in europe. | due to increased travel, climatic, and environmental changes, the incidence of tick-borne disease in both humans and animals is increasing throughout europe. therefore, extended surveillance tools are desirable. to accurately screen tick-borne pathogens (tbps), a large scale epidemiological study was conducted on 7050 ixodes ricinus nymphs collected from france, denmark, and the netherlands using a powerful new high-throughput approach. this advanced methodology permitted the simultaneous detect ... | 2014 | 25120960 |
| infections and coinfections of questing ixodes ricinus ticks by emerging zoonotic pathogens in western switzerland. | in europe, ixodes ricinus is the vector of many pathogens of medical and veterinary relevance, among them borrelia burgdorferi sensu lato and tick-borne encephalitis virus, which have been the subject of numerous investigations. less is known about the occurrence of emerging tick-borne pathogens like rickettsia spp., babesia spp., "candidatus neoehrlichia mikurensis," and anaplasma phagocytophilum in questing ticks. in this study, questing nymph and adult i. ricinus ticks were collected at 11 si ... | 2012 | 22522688 |
| whole-genome sequences of borrelia bissettii, borrelia valaisiana, and borrelia spielmanii. | it has been known for decades that human lyme disease is caused by the three spirochete species borrelia burgdorferi, borrelia afzelii, and borrelia garinii. recently, borrelia valaisiana, borrelia spielmanii, and borrelia bissettii have been associated with lyme disease. we report the complete genome sequences of b. valaisiana vs116, b. spielmanii a14s, and b. bissettii dn127. | 2012 | 22207749 |
| detection of a large linear plasmid in borrelia spielmanii isolate. | borrelia spielmanii belongs to human pathogenic species within the borrelia burgdorferi sensu lato complex in europe, which is a causative agent of lyme disease. so far, the human disease caused by b. spielmanii has been associated with skin manifestations. the aim of the study was to analyze 4 human b. spielmanii isolates by pulsed-field gel electrophoresis and to localize genes of 3 important borrelia proteins: ospa, dbpa, and vlse. the analysis revealed variation within linear plasmid profile ... | 2011 | 21491987 |
| reservoir competence of various rodents for the lyme disease spirochete borrelia spielmanii. | to determine whether the pathogenic lyme disease spirochete borrelia spielmanii is adapted exclusively to garden dormice, we compared the reservoir competence of various rodent species for this spirochete, including sympatric and peridomestic rodents. the different kinds of rodents varied in their attractiveness to nymphal ticks and their level of susceptibility to tick-borne b. spielmanii infection, but only the edible dormouse appeared to be refractory. although hazel dormice and norway rats b ... | 2011 | 21460106 |
| evaluation of a real-time pcr assay for the detection, genotyping, and quantification of borrelia burgdorferi sensu lato in ixodes ticks in a routine laboratory setting. | testing of saved ixodes ticks for the presence of borrelia burgdorferi after biting a patient may be helpful to assess the risk of bacterial transmission and to decide upon preventive antibiotic treatment. a commercially available real-time pcr assay for the identification and quantification of borrelia burdorferi sensu lato species (lightmix kit for the detection of borrelia spp., tib molbiol) was evaluated. | 2011 | 21391467 |
| prevalence and genetic heterogeneity of borrelia burgdorferi sensu lato in ixodes ticks in belgium. | borrelia burgdorferi sensu lato is a genetically diverse group of spirochetes that includes the agent of lyme borreliosis in which genospecies tend to be associated with specific clinical features. the aim of the study was to determine the prevalence and genetic diversity of borrelia burgdorferi sensu lato in 524 ticks collected in woods of a western province of belgium. presence of spirochetes in ticks was determined by phase contrast microscopy. the mean infection rate of ticks was 12.0%. vari ... | 2010 | 21128558 |
| [analysis of borrelia burgdorferi genostrains among patients with lyme disease]. | lyme borreliosis is a zoonose which can be transmitted to the humans during ixodes tick bite to the skin. the disease is caused by bacteria--spirochetes of the borrelia species, classified as borrelia burgdorferi strain. in poland lyme borreliosis is caused by borrelia garinii, borrelia afzelii and borrelia burgdorferi sensu stricto. the presence of borrelia spielmani was recently reported. | 2009 | 21033412 |
| establishment of a minor groove binder-probe based quantitative real time pcr to detect borrelia burgdorferi sensu lato and differentiation of borrelia spielmanii by ospa-specific conventional pcr. | abstract: | 2010 | 20698952 |
| functional characterization of borrelia spielmanii outer surface proteins that interact with distinct members of the human factor h protein family and with plasminogen. | acquisition of complement regulator factor h (cfh) and factor h-like protein 1 (cfhl1) from human serum enables borrelia spielmanii, one of the etiological agents of lyme disease, to evade complement-mediated killing by the human host. up to three distinct complement regulator-acquiring surface proteins (crasps) may be expressed by serum-resistant b. spielmanii, each exhibiting an affinity for cfh and/or cfhl1. here, we describe the functional characterization of the 15-kda crasps of b. spielman ... | 2010 | 19858303 |
| songbirds as general transmitters but selective amplifiers of borrelia burgdorferi sensu lato genotypes in ixodes rinicus ticks. | we investigated to what extent a european songbird (parus major) selectively transmits and amplifies borrelia burgdorferi s.l. bacteria. borrelia-naïve birds were recurrently exposed to ixodes ricinus nymphs carrying a community of more than 34 5s-23s genotypes belonging to five genospecies (borrelia garinii, borrelia valaisiana, borrelia afzelii, b. burgdorferi s.s. and borrelia spielmanii). fed ticks were screened for borrelia after moulting. we found evidence for co-feeding transmission of av ... | 2014 | 24118930 |
| geodemographic analysis of borrelia burgdorferi sensu lato using the 5s-23s rdna spacer region. | lyme borreliosis is the predominant tick-borne disease in the northern hemisphere, with considerable heterogeneity in clinical manifestations. here, we evaluated one genetic marker for its use in population genetic based analysis. for that we collected molecular and epidemiological records of borrelia burgdorferi sensu lato isolates from ticks, animals and humans at various sites in the netherlands and worldwide. | 2013 | 23602839 |
| prevalence of borrelia burgdorferi s. l. in ixodes ricinus ticks from four localities in bavaria, germany. | as a part of a larger survey a total of 599 ixodes ricinus ticks collected from four locations (neustadt an der waldnaab, amberg, poppenricht and lintach) in the north of the upper palatinate in bavaria were investigated for infection with borrelia burgdorferi sensu lato (s. l.) species using the 5s-23s intergenic spacer of rrna gene as a target in a nested pcr (rijpkema et al., 1995) and a sequencing method. overall, 15.8% ticks were infected with b. burgdorferi s. i. borrelia afzelii (43.1%) w ... | 2012 | 23045802 |
| identification of borrelia burgdorferi genospecies isolated from ixodes ricinus ticks in the south moravian region of the czech republic. | during 2008-2012, a total of 466 ticks ixodes ricinus removed from humans were collected and tested for the presence of borrelia burgdorferi sensu lato (bbsl). ticks were collected in all districts of the south moravian region of the czech republic (cz). | 2015 | 26706968 |
| lyme borreliosis spirochetes and spotted fever group rickettsiae in ixodid ticks from pianosa island, tuscany archipelago, italy. | a study on tick fauna and tick-borne pathogens was undertaken in pianosa, an island in the tuscany archipelago that constitutes an important stopping and nesting point for migratory birds. ticks were removed from feral cats and a few terrestrial birds, and host-seeking ticks were collected by dragging. a total of 89 ticks were found on animals: 57 ixodes ventalloi gil collado, 1936 and 32 ixodes acuminatus neumann, 1901. host-seeking ticks were 354 hyalomma spp. larvae and 18 hyalomma spp. adult ... | 2013 | 23289398 |
| prevalence and distribution of borrelia and babesia species in ticks feeding on dogs in the u.k. | ticks were collected during march-july 2015 from dogs by veterinarians throughout the u.k. and used to estimate current prevalences and distributions of pathogens. dna was extracted from 4750 ticks and subjected to polymerase chain reaction and sequence analysis to identify borrelia burgdorferi sensu lato (spirochaetales: spirochaetaceae) and babesia (piroplasmida: babesiidae) species. of 4737 ticks [predominantly ixodes ricinus linneaus (ixodida: ixodidae)], b. burgdorferi s.l. was detected in ... | 2017 | 28846148 |
| borrelia burgdorferi sensu lato in ixodes longiscutatus ticks from brazilian pampa. | borrelia burgdorferi sensu lato (s.l.) complex includes the agents of lyme disease/borreliosis in north america, europe, and asia, such borrelia burgdorferi sensu stricto, borrelia afzelii, borrelia garinii, borrelia bavariensis, borrelia spielmanii, borrelia bissettiae, and borrelia mayonii. in 2013 b. burgdorferi s.l. was reported for the first time in the neotropical region, from ixodes aragaoi ticks in uruguayan pampa. in addition, from 2011 to 2016, 17 suspected human cases of borreliosis-l ... | 2017 | 28847698 |
| approaches for reverse line blot-based detection of microbial pathogens in ixodes ricinus ticks collected in austria and impact of the chosen method. | ticks transmit a large number of pathogens capable of causing human disease. in this study, the pcr-reverse line blot (rlb) method was used to screen for pathogens in a total of 554 ixodes ricinus ticks collected from all provinces of austria. these pathogens belong to the genera borrelia, rickettsiae, anaplasma/ehrlichia (including "candidatus neoehrlichia"), babesia, and coxiella the pathogens with the highest detected prevalence were spirochetes of the borrelia burgdorferisensu lato complex, ... | 2017 | 28455331 |
| in vitro susceptibility of borrelia spielmanii to antimicrobial agents commonly used for treatment of lyme disease. | ten isolates of the recently delineated genospecies borrelia spielmanii were tested against antimicrobial agents used to treat lyme disease and compared to eight isolates of the other three human-pathogenic borrelial genospecies. despite some small but significant differences in four out of eight antibiotic agents, the susceptibility pattern of b. spielmanii mainly parallels that of the other known human-pathogenic members of the b. burgdorferi sensu lato complex. | 2009 | 19075048 |