| phage infection, transfection and transformation of mycobacterium avium complex and mycobacterium paratuberculosis. | mycobacterium avium complex strains and mycobacterium paratuberculosis are closely related intracellular pathogens affecting humans and animals. m. avium complex infections are a leading cause of morbidity and mortality in aids patients, and m. paratuberculosis is the agent of johne's disease in ruminants. genetic manipulation of these micro-organisms would facilitate the understanding of their pathogenesis, the construction of attenuated vaccine strains and the development of new drugs and trea ... | 1995 | 7773411 |
| mycobacteriophage tm4: genome structure and gene expression. | mycobacteriophage tm4 is a dsdna-tailed phage that infects both fast-growing and slow-growing strains of mycobacteria. while tm4 has been used extensively for the construction of mycobacterial shuttle phasmids and for the delivery of reporter genes and transposons into mycobacterial cells, little is known about its genetics or molecular biology. we describe here the complete 52,797 bp genome sequence of tm4 and a map of its genome organization. while not a close relative of other mycobacteriopha ... | 1998 | 10645443 |
| expression of mycobacteriophage ms6 lysis genes is driven by two sigma(70)-like promoters and is dependent on a transcription termination signal present in the leader rna. | a mycobacteriophage ms6 strong promoter region (p(lys)) was isolated by using transcriptional fusions with the lacz reporter gene. two tandem sigma(70)-like promoter sequences (p1 and p2) were found in this region. dna sequencing of the promoter downstream region revealed a 214-bp leader sequence followed by five adjacent coding regions of 231 bp (orf1), 1,152 bp (orf2), 996 bp (orf3), 231 bp (orf4), and 372 (orf5). orf1 has the potential to encode a 77-amino-acid protein which revealed similari ... | 2002 | 12003945 |
| a peptidoglycan hydrolase motif within the mycobacteriophage tm4 tape measure protein promotes efficient infection of stationary phase cells. | the predominant morphotype of mycobacteriophage virions has a dna-containing capsid attached to a long flexible non-contractile tail, features characteristic of the siphoviridae. within these phage genomes the tape measure protein (tmp) gene can be readily identified due to the well-established relationship between the length of the gene and the length of the phage tail--because these phages typically have long tails, the tmp gene is usually the largest gene in the genome. many of these mycobact ... | 2006 | 17083467 |
| tape measure protein having mt3 motif facilitates phage entry into stationary phase cells of mycobacterium tuberculosis. | tape measure protein (tmp) having mt3 motif in mycobacteriophage tm4 genome has been reported to enable the phage infection of mycobacterium smegmatis during stationary phase. in the present work looking at eight additional mycobacteriophage genomes by in silico analysis, six of them have been found to possess mt3 motif in tmp. the absence of mt3 motif in che12 and d29 probably makes them incapable of infecting stationary phase cells of mycobacterium tuberculosis which was experimentally evaluat ... | 2008 | 18479969 |
| insights into the function of the whib-like protein of mycobacteriophage tm4--a transcriptional inhibitor of whib2. | whib-like proteins of actinomycetes are known to co-ordinate iron-sulfur (fe-s) clusters and are believed to have regulatory functions in many essential bacterial processes. the systematic determination of the genome sequences of mycobacteriophages has revealed the presence of several whib-like genes in these viruses. here we focussed on the whib-like protein of mycobacteriophage tm4, whibtm4. we provide evidence that this viral protein is capable of co-ordinating a fe-s cluster. the uv-visible ... | 2010 | 20545868 |
| cloning and expression of a mureinolytic enzyme from the mycobacteriophage tm4. | in this study, we describe the characterization, cloning, expression and purification of the lysin a gene of the mycobacteriophage tm4. the gene tm4_gp29 (gp29) is a 1644-bp gene that codes for a 58.6-kda protein and contains peptidoglycan recognition protein, zn-binding and amidase catalytic domains. the gene was cloned into escherichia coli using the 'his-tag' pqe60 vector. after affinity chromatography-mediated purification, the protein was concentrated and visualized using sodium dodecyl sul ... | 2010 | 20727013 |
| cluster k mycobacteriophages: insights into the evolutionary origins of mycobacteriophage tm4. | five newly isolated mycobacteriophages--angelica, crimd, adephagia, anaya, and pixie--have similar genomic architectures to mycobacteriophage tm4, a previously characterized phage that is widely used in mycobacterial genetics. the nucleotide sequence similarities warrant grouping these into cluster k, with subdivision into three subclusters: k1, k2, and k3. although the overall genome architectures of these phages are similar, tm4 appears to have lost at least two segments of its genome, a centr ... | 2011 | 22053209 |