| purification and characterization of the alpha-1,3-mannosylmannose-recognizing lectin of crocus vernus bulbs. | a unique mannose-binding lectin, highly specific for terminal man(alpha1,3)man groups, was isolated from bulbs of crocus (crocus vernus all.). the lectin failed to bind to a mannose affinity column and was purified by simple gel permeation chromatography (sephacryl s200). the purified lectin, obtained in crystalline form, had a molecular mass of 44 kda on gel filtration and showed a single peptide band with a molecular mass of 11 kda on sds-polyacrylamide gel electrophoresis, indicating it to be ... | 1997 | 9325257 |
| quality control of saffron (crocus sativus l.): development of scar markers for the detection of plant adulterants used as bulking agents. | a method based on sequence-characterized amplified regions (scars) was developed from random amplified polymorphic dna markers (rapds) specific for arnica montana l., bixa orellana l., calendula officinalis l., carthamus tinctorius l., crocus vernus l. (hill), curcuma longa l., and hemerocallis sp. to detect these common bulking agents in commercial saffron (crocus sativus). the method enabled the unequivocal detection of low amounts (up to 1%) of each adulterant, allowing the preemptive rejecti ... | 2012 | 22989071 |
| isolation, purification, crystallization and preliminary crystallographic studies of a chitinase from crocus vernus. | a chitinase has been isolated and purified from crocus vernus corms. n-terminal amino-acid sequence analysis of the approximately 30 kda protein showed 33% identity to narbonin, a seed protein from vicia narbonensis l. the c. vernus chitinase was crystallized by the hanging-drop vapour-diffusion method using peg 8000 as the main precipitant. the crystal belonged to the monoclinic space group c2, with unit-cell parameters a=172.3, b=37.1, c=126.4 å, β=127° and two molecules per asymmetric unit. d ... | 2011 | 21393839 |
| analysis of genetic diversity in crocuses with carpathian basin origin using aflp-markers. | crocus taxonomy has until now been based primarily on morphology, taking chromosome numbers into consideration. the genetics and genome structure of the genus, the relationships and diversity within the genus are not well known. amplified fragment length polymorphism (aflp) is a whole genome approach to study genetic variation that is gaining in popularity for lower-level systematics. the present study employed the aflp technique for analyzing relationships among taxa of the crocus genus (partic ... | 2010 | 21565773 |
| low temperature maximizes growth of crocus vernus (l.) hill via changes in carbon partitioning and corm development. | in crocus vernus, a spring bulbous species, prolonged growth at low temperatures results in the development of larger perennial organs and delayed foliar senescence. because corm growth is known to stop before the first visual sign of leaf senescence, it is clear that factors other than leaf duration alone determine final corm size. the aim of this study was to determine whether reduced growth at higher temperatures was due to decreased carbon import to the corm or to changes in the partitioning ... | 2009 | 19403850 |
| pollen-wall proteins: electron-microscopic localization of acid phosphatase in the intine of crocus vernus. | | 1971 | 5087655 |
| cloning and characterization of a monocot mannose-binding lectin from crocus vernus (family iridaceae). | the molecular structure and carbohydrate-binding activity of the lectin from bulbs of spring crocus (crocus vernus) has been determined unambiguously using a combination of protein analysis and cdna cloning. molecular cloning revealed that the lectin called c. vernus agglutinin (cva) is encoded by a precursor consisting of two tandemly arrayed lectin domains with a reasonable sequence similarity to the monocot mannose-binding lectins. post-translational cleavage of the precursor yields two equal ... | 2000 | 10931189 |
| repetitive dna sequences in crocus vernus hill (iridaceae): the genomic organization and distribution of dispersed elements in the genus crocus and its allies. | eight clones of repetitive dna were isolated from crocus vernus hill. the genomic organization of the clones was analyzed by in situ hybridization to c. vernus and southern hybridization to a range of crocus and other species. seven clones were used for in situ hybridization. sequence analysis showed that all eight clones were nonhomologous, and thus represented eight different sequence-families. in situ hybridization showed that six were dispersed in high copy numbers on all chromosomes of the ... | 2000 | 11081982 |
| the genomic organization and evolutionary distribution of a tandemly repeated dna sequence family in the genus crocus (iridaceae). | from a recombinant dna-library from crocus vernus, two closely related clones of highly repetitive dna, pcvkb7 and pcvkb8, were sequenced and their genomic distribution and organization were investigated by southern and in situ hybridization. the lengths of the clones were 181 and 178 bp respectively; the sequences were approximately 85% identical, and thus belonged to a sequence family, named the pcvkb8-family. no homologous sequences were found in the databases (blast made may 2004). the prese ... | 2004 | 15383076 |
| reversible inhibition of the pollen germination and the stigma penetration in crocus vernus ssp. vernus (iridaceae) following fumigations with no2, co, and o3 gases. | we assessed the pollen hydration, the pollen germination, and the stigma papilla penetration of crocus vernus subsp. vernus (iridaceae) after 2 h fumigations with o (3), no (2), and co gases within humidified (90 - 100 % rh) box experiments. when the pollen and the pistil were separately fumigated, the pollen retained the capacity to emit a tube which penetrated papilla, and the stigma papillae retained the receptivity; when the pistils were first pollinated and then fumigated, the capacity of p ... | 2007 | 17564951 |
| structural analysis of β-prism lectin from colocasia esculenta (l.) s chott. | the mannose-binding β-prism colocasia esculenta lectin (β-pcl) was purified from tubers using ion exchange chromatography. the purified β-pcl appeared as a single band of ∼12kda on sds-page. β-pcl crystallizes in trigonal space group p3121 and diffracted to a resolution of 2.1å. the structure was solved using molecular replacement using crocus vernus lectin (pdb: 3mez) as a model. from the final refined model to an r-factor of 16.5% and an rfree of 20.4%, it has been observed that the biological ... | 2016 | 27262515 |
| suitability of different pollen as alternative food for the predatory mite amblyseius swirskii (acari, phytoseiidae). | the predacious mite amblyseius swirskii athias-henriot is used as a biological control agent against various pests in greenhouses. pollen offered as supplementary food is reported to improve their fast establishment and performance. however, the nutritional suitability of different pollens for a. swirskii is not sufficiently known yet. pollens of 21 plant species were offered to the mites as exclusive food during preimaginal development. preimaginal mortality and developmental time have been ass ... | 2013 | 23670826 |
| [the smooth endoplasmic reticulum develops in mesophyll cells of early-spring ephemerals]. | a study was made of the mesophyll cell ultrastructure of early-spring ephemerals scilla sibirica, crocus vernus, galanthus caucasicus, g. plicatus, leucojum vernum, muscari szovitsianum, ornithogalum balansae of summer-vegetating. scilla scilloides and of s. sibirica growing in greenhouse. the highly developed smooth endoplasmic reticulum (ser) was a distinctive feature of the early-spring ephemeral mesophyll cell during leaf growth (cell expansion). direct connections of ser-compartments with t ... | 2000 | 11204644 |