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in vitro determination of the spermicidal activity of plant saponins.the plant kingdom might yield an effective antifertility drug. a mentha arvensis l. (labiatae) fraction with uterotonic activity was isolated, and was found to be active on the nonpregnant as well as the pregnant rat uterus. according to folklore medicine, the mexican plant montanoa tomentosa cerv. (zoapatle) possesses antifertility activity in women. the effect of various isolated preparations from this plant on early pregnancy were investigated in serveral rodent species including the mouse ...19854080814
tumor inhibitors. xxi. active principles of acer negundo and cyclamen persicum. 19676032773
[serious plant poisonings in switzerland 1966-1994. case analysis from the swiss toxicology information center].to analyze the types, frequency and severity of plant poisonings in switzerland over 29 years.19968711457
quantification of mycelium of botrytis spp. and the antagonist ulocladium atrum in necrotic leaf tissue of cyclamen and lily by fluorescence microscopy and image analysis.abstract a technique was developed to localize and quantify the internal mycelial colonization of necrotic leaf tissue of cyclamen (cyclamen persicum) or lily (lilium) by pathogenic botrytis spp. and the antagonist ulocladium atrum. this technique allows investigation of competitive substrate colonization by both fungi, which is a key process for biological control of botrytis spp. by u. atrum. a combination of differential fluorescent labeling and image analysis was applied on cryostat sections ...199918944729
phenotypical differences among b. cinerea isolates from ornamental plants.b. cinerea is a common pathogenic fungus which causes botrytis blight (grey mould) in most ornamental plants. it may be responsible for serious preharvest diseases and postharvest losses in fruits, vegetables and flowers. in this work, several b. cinerea isolates from ornamental plants (chamelaucium uncinatum, pelargonium x hortorum, euphorbia pulcherrima, lantana camara, lonicera japonica, hydrangea macrophylla, and cyclamen persicum) affected by botrytis blight in the south of spain were studi ...200819226749
mycobiota from cyclamen persicum and its interaction with botrytis cinerea.sixty-six fungi isolated from cyclamen phylloplanes were identified and assessed in vitro for antagonism to b. cinerea on leaves, petals, petioles and peduncles. the estimation of pathogen conidial production was used as indicator of biocontrol ability of each of the strains. they were classified by cluster analysis resulting in four categories according to their behavior in the different organs. the most promising category included 34 isolates that significantly reduced pathogen inoculum in all ...200919397189
temperature-dependent growth of botrytis cinerea isolates from potted plants.botrytis cinereo is a common aggressive saprophyte fungus which also invades injured plant tissues, causing botrytis blight (grey mould) in many ornamental plants, including potted flowering plants. several b. cinerea isolates from potted plants (pelargonium x hortorum, lantana camara, lonicera japonica, hydrangea macrophylla, and cyclamen persicum) affected by botrytis blight in the south of spain were studied and identified by pcr. the isolates showed phenotypic differences between them, as pr ...200920222557
triterpene saponins from cyclamen persicum.a new triterpene saponin 3-o-beta-d-glucopyranosyl-(1 --> 4)-alpha-l-arabinopyranosyl-16alpha-hydroxy-13beta,28-epoxy-oleanan-30-al (1), along with four known triterpene glycosides (2-5) were isolated from cyclamen persicum. their structures were characterized by a combination of 1d- and 2d-nmr (1h-1h cosy, tocsy, noesy, hsqc, and hmbc) and ms spectrocopic data. the cytotoxicity of compounds 2 and 4 was evaluated using two human colon cancer cell lines ht-29 and hct 116.201020734932
fusarium foetens, a new species pathogenic to begonia elatior hybrids (begonia x hiemalis) and the sister taxon of the fusarium oxysporum species complex.a new disease recently was discovered in begonia elatior hybrid (begonia × hiemalis) nurseries in the netherlands. diseased plants showed a combination of basal rot, vein yellowing and wilting and the base of collapsing plants was covered by unusually large masses of fusarium macroconidia. a species of fusarium was isolated consistently from the discolored veins of leaves and stems. it differed morphologically from f. begoniae, a known agent of begonia flower, leaf and stem blight. the fusarium ...200421148861
effects of paclobutrazol on botrytis cinerea isolates obtained from potted plants.the growth of different isolates of botrytis cinerea, collected from potted plants affected by botrytis blight in southern spain during recent years, was studied. these isolates, which show wide phenotypic differences when grown in vitro, are differentially affected by growth temperature, gibberellic acid, and paclobutrazol--an efficient plant growth retardant used widely in nursery potted plants to reduce plant size, favouring compactness, a more intense green foliage and increased stress toler ...201021534481
effects of indole-3-acetic acid on botrytis cinerea isolates obtained from potted plants.we study the growth of different isolates of botrytis cinerea collected from potted plants which were affected by botrytis blight in southern spain during recent years. these isolates, which show widely phenotypic differences when grown in vitro, are differentially affected by growth temperature, gibberellic acid applications and paclobutrazol, an efficient plant growth retardant and fungicide at the same time. in this work, we have evaluated the effect of the auxin indole-3-acetic acid (iaa) do ...201122702183
multi-petal cyclamen flowers produced by agamous chimeric repressor expression.cyclamen persicum (cyclamen) is a commercially valuable, winter-blooming perennial plant. we cloned two cyclamen orthologues of agamous (ag), cpag1 and cpag2, which are mainly expressed in the stamen and carpel, respectively. cyclamen flowers have 5 petals, but expression of a chimeric repressor of cpag1 (cpag1-srdx) caused stamens to convert into petals, resulting in a flower with 10 petals. by contrast, cpag2-srdx only caused incomplete formation of stamens and carpels. expression in arabidops ...201324026510
occupational respiratory allergy to cyclamen pollen: a case report.a case of cyclamen (cyclamen persicum) pollen allergy is described on a flower cultivator. the diagnosis was assessed by skin test, methacoline test, nasal provocation test and rast. sds-page and immunoblotting have shown an intense response against a 18 kda component and a weaker one at 100 kda. a rast inhibition against cupressus sempervirens and acacia floribundia showed no identity with cyclamen. this case confirm the cyclamen allergenity, in professional exposure. the nasal challenge prove ...200616752694
metabolite profiling of somatic embryos of cyclamen persicum in comparison to zygotic embryos, endosperm, and testa.somatic embryogenesis has been shown to be an efficient in vitro plant regeneration system for many crops such as the important ornamental plant cyclamen persicum, for which this regeneration pathway of somatic embryogenesis is of interest for the vegetative propagation of parental lines as well as elite plants. however, somatic embryogenesis is not commercially used in many crops due to several unsolved problems, such as malformations, asynchronous development, deficiencies in maturation and ge ...201526300898
in vitro cytotoxic and anticlastogenic activities of saxifragifolin b and cyclamin isolated from cyclamen persicum and cyclamen libanoticum.the genus cyclamen l. (primulaceae) is rich in saponins known to have interesting biological activities.201424649909
proteomic and histological analyses of endosperm development in cyclamen persicum as a basis for optimization of somatic embryogenesis.the endosperm plays an important role for the development of zygotic embryos, while somatic embryos lack a seed coat and endosperm and often show physiological disorders. this study aims at elucidating the cellular and physiological processes within the endosperm of the ornamental species cyclamen persicum mill. histological analyses were performed from 0 to 11 weeks after pollination (wap). at 3wap, a syncytium was clearly visible with a globular zygotic embryo. from 4wap, cellularization of th ...201323352402
influence of arbuscular mycorrhiza on the growth and antioxidative activity in cyclamen under heat stress.the influence of the arbuscular mycorrhizal (am) fungus, glomus fasciculatum, on the growth, heat stress responses and the antioxidative activity in cyclamen (cyclamen persicum mill.) plants was studied. cyclamen plants (inoculated or not with the am fungus) were placed in a commercial potting media at 17-20 °c for 12 weeks in a greenhouse and subsequently subjected to two temperature conditions in a growth chamber. initially, plants were grown at 20 °c for 4 weeks as a no heat stress (hs-) cond ...201323334657
thermotolerant cyclamen with reduced acrolein and methyl vinyl ketone.reduced levels of trienoic fatty acids (tas) in chloroplast membranes induce thermotolerance in several plant species, but the underlying mechanisms remain unclear. ta peroxidation in plant cell membranes generates cytotoxic, ta-derived compounds containing α,β-unsaturated carbonyl groups. the relationship between low ta levels and the amounts of cytotoxic ta-derived compounds was examined using thermotolerant transgenic cyclamen (cyclamen persicum mill.) with low ta contents. changes in the lev ...201222511805
from callus to embryo: a proteomic view on the development and maturation of somatic embryos in cyclamen persicum.in this study, the proteome structures following the pathway in somatic embryogenesis of cyclamen persicum were analysed via high-resolution 2d-sds-page with two objectives: (1) to identify the significant physiological processes during somatic embryogenesis in cyclamen and (2) to improve the maturation of somatic embryos. therefore, the effects of maturation-promoting plant growth regulator abscisic acid (aba) and high sucrose levels on torpedo-shaped embryos were investigated. in total, 108 pr ...201222127736
interspecific somatic hybrids between cyclamen persicum and c. coum, two sexually incompatible species.by applying polyethylene glycol (peg)-mediated protoplast fusion, the first somatic hybrids were obtained between cyclamen persicum (2n = 2x = 48) and c. coum (2n = 2x = 30)-two species that cannot be combined by cross breeding. heterofusion was detected by double fluorescent staining with fluorescein diacetate and scopoletin. the highest heterofusion frequencies (of about 5%) resulted from a protocol using a protoplast density of 1 × 10(6)/ml and 40% peg. the dna content of c. coum was estimate ...201222108718
two serk genes are markers of pluripotency in cyclamen persicum mill.the genetic basis of stem cell specification in somatic embryogenesis and organogenesis is still obscure. somatic embryogenesis receptor-like kinase (serk) genes are involved in embryogenesis and organogenesis in numerous species. in vitro culture of cyclamen persicum immature ovules provides a system for investigating stem cell formation and maintenance, because lines forming either organs or embryos or callus without organs/embryos are available for the same cultivar and plant growth regulator ...201221976770
enolases: storage compounds in seeds? evidence from a proteomic comparison of zygotic and somatic embryos of cyclamen persicum mill.somatic embryogenesis is well established for the economic relevant ornamental crop cyclamen and thus could supplement the elaborate propagation via seeds. however, the use of somatic embryogenesis for commercial large scale propagation is still limited due to physiological disorders and asynchronous development within emerged embryos. to overcome these problems, profound knowledge of the physiological processes in cyclamen embryogenesis is essential. thus, the proteomes of somatic and zygotic e ...201121249422
large impact of the apoplast on somatic embryogenesis in cyclamen persicum offers possibilities for improved developmental control in vitro.clonal propagation is highly desired especially for valuable horticultural crops. the method with the potentially highest multiplication rate is regeneration via somatic embryogenesis. however, this mode of propagation is often hampered by the occurrence of developmental aberrations and non-embryogenic callus. therefore, the developmental process of somatic embryogenesis was analysed in the ornamental crop cyclamen persicum by expression profiling, comparing different developmental stages of emb ...201020426818
clonal propagation of cyclamen persicum via somatic embryogenesis.cyclamen (cyclamen persicum) is an economically important ornamental pot plant with local use as cut flower as well. traditionally, it is propagated via seeds, but interest is given in vegetative propagation of parental lines as well as superior single plants. somatic embryogenesis is an efficient in vitro propagation method for many cyclamen cultivars. starting from ovules of unpollinated flowers, callus is induced and propagated in a medium containing 2,4-dichlorophenoxyacetic acid (2,4-d) and ...201020099110
[cryopreservation of cyclamen persicum mill. callus].in this paper we studied cryopreservation of cyclamen persicum mill. callus to avoid variations produced by sub-culture. the callus in the logarithmic phase after sub-culture were used for experiments. firstly, the callus were pre-cultured in culture-medium containing 4%, 6% or 8% sucrose for different time periods, transferred to different cryoprotectants to directly cryopreserve or incubated for 2 hours at -20 degrees c, then submersed in liquid nitrogen, lastly thawed rapidly in a waterbath a ...200818589831
traditional knowledge of wild edible plants used in palestine (northern west bank): a comparative study.a comparative food ethnobotanical study was carried out in fifteen local communities distributed in five districts in the palestinian authority, pa (northern west bank), six of which were located in nablus, two in jenin, two in salfit, three in qalqilia, and two in tulkarm. these are among the areas in the pa whose rural inhabitants primarily subsisted on agriculture and therefore still preserve the traditional knowledge on wild edible plants.200818474107
the anatomy of the stigma and style from cyclamen persicum (mill.) cv. "pure white" and its relation to pollination success.old flowers of cyclamen generate few or no seeds. to understand the pollination problems of cyclamen we investigate the general anatomy of the stigma and the style of cyclamen persicum by scanning electron microscopy at different stages of floral maturity. our investigations confirm that there is a hollow style. against data commonly found in the literature, we present evidence of pollen germination and tube growth that show the stigma is not outside the style but inside it. furthermore the matu ...200717099843
comparative analysis of cell cycle events in zygotic and somatic embryos of cyclamen persicum indicates strong resemblance of somatic embryos to recalcitrant seeds.embryo development and germination of cyclamen persicum have been comparatively characterized for zygotic and somatic embryos with regard to mitotic activity and morphology in order to identify developmental abnormalities in somatic embryogenesis. zygotic embryo development proved to be highly synchronous with distinct periods of cell division, cell elongation and embryo maturation within a total period of 17 weeks of seed development. somatic embryo development was accomplished within only 3 we ...200616496150
proteomic analyses of somatic and zygotic embryos of cyclamen persicum mill. reveal new insights into seed and germination physiology.in the horticulturally important ornamental species cyclamen persicum mill., somatic embryogenesis is an efficient vegetative propagation method and the development of artificial seeds is an ultimate aim. this study aims at a systematic comparison of the proteomes of zygotic embryos, somatic embryos grown in liquid medium containing 30 or 60 g l(-1) sucrose, germinating embryos of both types and endosperm in order to obtain novel insights into seed and germination physiology. using high resoluti ...200616496095
two plant fish-poisons in lebanon.the use of a powder for poisoning sea fish off lebanon is reported for the first time. the powder is prepared from the seeds of storax (styrax officinalis l) and tubers of common cyclamen (cyclamen persicum mill.), both species growing wild in lebanon. references to the properties of these species are provided.200415587258
cryopreservation of embryogenic suspension cultures of cyclamen persicum mill.we have developed a simple protocol for the cryopreservation of embryogenic suspension cultures of cyclamen persicum. embryogenic suspension cultures in the linear growth phase 7-10 days after subculture were used for cryopreservation. of the different cryoprotectants tested during a 2-day pre-culture, 0.6 m sucrose resulted in the highest re-growth rates of 75%. an additional pre-treatment with 0.6 m sucrose and 10% dmso (dimethylsulfoxide) for 1 h also positively affected re-growth. microscopi ...200415160283
shoot regeneration in long-term callus cultures derived from mature flowering plants of cyclamen persicum mill.in long-term callus cultures of cyclamen persicum mill. two types of tissue could be distinguished. one type featured a brown suberised outer layer and was poorly organogenic. the other type was yellowish in appearance and gave rise to many shoot buds. both types co-existed on the same callus, the former prevailing. selection for organogenic tissue resulted in cultures yielding approximately three times more petioles than random subcultures. callus-derived shoots could be rooted and established ...199624178470
does pollination induce corolla abscission of cyclamen flowers by promoting ethylene production?very low ethylene production rates were measured in nonpollinated cyclamen persicum mill flowers, and no change in production was observed during the whole life span of the flower until death. normal senescence was accompanied by a gradual discoloration and loss of turgor followed by wilting. pollination induced a dramatic increase in ethylene evolution, culminating in a peak 4 days after pollination, and abscission of the corolla on that day. silver-thiosulfate, an inhibitor of ethylene action, ...198416663740
[research on plant viruses. virus note vii. speckling of the flower of the cyclamen (cyclamen persicum l.)]. 196414139171
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