| effects of intradermal gamma-interferon in cutaneous leishmaniasis. | the clinical and immunological evolution of lesions in cutaneous leishmaniasis was assessed after treatment with human recombinant gamma interferon (rifn-gamma). 3 weeks after rifn-gamma treatment of lesions due to leishmania braziliensis guyanensis, 12/13 had become smaller compared with 6/13 control lesions; only 4 treated lesions were free of parasites. 9 of 13 l tropica lesions treated with rifn-gamma resolved completely within 4-8 weeks of treatment. an acute inflammatory reaction around tr ... | 1989 | 2566825 |
| first venezuelan record of lutzomyia umbratilis ward & fraiha, 1977 (diptera: psychodidae), a proven vector of leishmania braziliensis guyanensis. | | 1985 | 3832500 |
| isoenzyme patterns of leishmania isolates from colombia. | promastigotes from four cutaneous leishmaniasis cases from colombia were tested by cellulose acetate electrophoresis using nine enzyme systems. the isoenzyme profiles of the colombian isolates were indistinguishable from each other and from panamanian leishmania braziliensis panamensis controls, but were distinct from an isolate of leishmania braziliensis guyanensis from brazil and three isolates from the leishmania mexicana complex for the enzyme phosphogluconate dehydrogenase. | 1985 | 4003660 |
| identification of a major 72 kilodalton surface antigen in twelve isolates of leishmania braziliensis braziliensis. | the study of the surface antigens of leishmania braziliensis braziliensis revealed a great homogeneity among ten strains isolated from bolivia and two reference strains from brazil and belize. a 72 kda major protein, present in all l. b. braziliensis strains, was recognized by both cutaneous and mucocutaneous human sera, but was not recognized by kala-azar and chagasic sera. no cross-reactive antigens were found among strains of leishmania braziliensis guyanensis, leishmania braziliensis panamen ... | 1987 | 3627165 |
| ornithine decarboxylase and trypanothione reductase genes in leishmania braziliensis guyanensis. | ornithine decarboxylase and trypanothione reductase are the key enzymes in polyamine and trypanothione metabolism in kinetoplastids. using a heterologous trypanosoma brucei brucei probe for ornithine decarboxylase and a mixed synthetic probe of 29 oligonucleotides for trypanothione reductase, we have detected the putative genes for these enzymes by southern blot hybridization using genomic dna of leishmania braziliensis guyanensis mhom/sr/80/cumc 1. the trypanothione reductase probe was construc ... | 1989 | 2810144 |
| isoenzyme characterization of 112 leishmania isolates from french guiana. | 112 leishmania isolates, obtained in french guiana from human lesions, phlebotomine sandflies and wild mammals, were characterized by isoenzyme electrophoresis. leishmania braziliensis guyanensis and l. mexicana amazonensis were found parasitizing different natural hosts. l.b. guyanensis was the dominant species (103 isolates) responsible for most of the human lesions (96.7%). based on variations observed in 2 enzymes, 3 distinct zymodemes were distinguished within the l.b. guyanensis taxon. | 1989 | 2617620 |
| mucosal disease caused by leishmania braziliensis guyanensis. | three leishmania strains were isolated from cutaneous and mucosal lesions of a colombian male. these strains, shown to be phenotypically identical based on isoenzyme polymorphisms and monoclonal antibody reactivity, were identified as leishmania braziliensis guyanensis. six clones of the mucosal strain were phenotypically identical to the 3 strains. the clinical presentation of the cutaneous lesions and the geographic origin of the infection were consistent with infection by l. b. guyanensis. | 1990 | 2301705 |
| matrix remodelling and fibroblast phenotype in early lesions of human cutaneous leishmaniasis. | the connective matrix participates directly in early pathological events observed in the cutaneous lesion of leishmaniasis, due to leishmania braziliensis guyanensis. a sample of 19 skin biopsies was examined by light and electron microscopy, in order to identify the matrix components (collagen isotypes i to iv, elastin and membrane associated proteins) of the dermal infiltrate, and the pattern of organization of the reparative connective matrix. an extensive remodelling process of apparently pa ... | 1991 | 1792187 |
| cell populations in the lesion of human cutaneous leishmaniasis: a light microscopical, immunohistochemical and ultrastructural study. | to characterize the in situ cellular immune response in localized cutaneous leishmaniasis (lcl), the authors studied frozen skin biopsies from 50 patients with lcl due to leishmania braziliensis guyanensis. a panel of 31 monoclonal antibodies was used, which defined the number and distribution of inflammatory cell subsets. skin inflammatory infiltrates were composed of t cells (with a local cd4/cd8 ratio of 1.05 +/- 0.7 vs 1.48 +/- 0.3 in peripheral blood), macrophages and a smaller number of b ... | 1992 | 1413489 |
| an electron microscopic and cytochemical detection of concanavalin a receptors on the cell membrane of leishmania braziliensis guyanensis. | promastigotes of leishmania braziliensis quyanensis cultivated in the nnn medium agglutinate with concanavalin a (con a). the protozoon was agglutinated at different concentrations of con a. maximal agglutination was obtained with 150 mug/ml. three types of agglutination were observed: flagellar-flagellar, flagellar-body and body-body. cell surface polysaccharides, glycoproteins or glycolipids were demonstrated using the periodic acid-thiosemicarbazide-silver proteinate technic. a con a-horserad ... | 1976 | 961000 |
| american cutaneous leishmaniasis in guyana, south america. | the 185 patients who presented at the dermatology clinic of georgetown public hospital, guyana, between 1992 and 1998, with skin ulcers indicative of american cutaneous leishmaniasis (acl) were retrospectively reviewed. the laboratory-confirmed cases of acl were identified and the corresponding data were analysed for risk factors such as age, gender, areas of residence and of possible exposure to the causative agent (leishmania braziliensis guyanensis), ethnic origin, longevity of the ulcers, an ... | 2001 | 11339884 |
| comparison of new and old world leishmanins in an endemic region of brazil. | the control of leishmaniasis depends on a knowledge of the magnitude of the disease and of exposure to it. delayed-type hypersensitivity testing can detect prior exposure to the parasite, but there is little agreement regarding the choice of an antigen for such testing. new and old world leishmanins were tested in a study of patients with confirmed prior cutaneous leishmaniasis (cl), patients with confirmed prior american visceral leishmaniasis (avl), and controls from areas in espírito santo, b ... | 1995 | 7620013 |
| the opossum, didelphis marsupialis (marsupialia: didelphidae), as a reservoir host of leishmania braziliensis guyanensis in the amazon basin of brazil. | a total of 52 opossums (six species) were examined for evidence of infection with leishmania in three different areas of forest near manaus, amazonas state, brazil. no infections were detected in 27 opossums from a region of relatively undisturbed forest, including specimens of didelphis marsupialis (18); metachirus nudicaudatus (four); monodelphis brevicaudata (one); marmosa cinerea (two); m. murina (one) and m. parvidens (one). of 15 d. marsupialis captured from a biological reserve, much dist ... | 1981 | 7324129 |
| leishmaniasis in brazil: xvi. isolation and identification of leishmania species from sandflies, wild mammals and man in north para state, with particular reference to l. braziliensis guyanensis causative agent of "pian-bois". | a total of 125 wild mammals (14 different species) were examined for evidence of infection with leishmania in an area of primary forest highly endemic for "pian-bois", due to leishmania braziliensis guyanensis, in north pará state, brazil. parasites isolated were characterized biologically, and biochemically on enzymic profiles. l. b. guyanensis was isolated from the viscera of one lesser anteater (tamandua tetradactyla) and one opossum (didelphis marsupialis), and the skin of one rodent (proech ... | 1981 | 7324128 |
| failure to cure leishmania braziliensis guyanensis cutaneous leishmaniasis with oral ketoconazole. | | 1986 | 3726992 |
| hosts of sandfly vectors of leishmania braziliensis guyanensis in the central amazon of brazil. | the blood meals of 2,569 phlebotomine sandflies from areas endemic for cutaneous leishmaniasis in the central amazon of brazil were tested by the microcapillary precipitin method to determine their vertebrate hosts. the two-toed sloth, choloepus didactylus, was the predominant host of two incriminated vectors of leishmania braziliensis guyanensis in the region, lutzomyia umbratilis and lu. anduzei (64.0% and 63.6%, respectively). the lu. "shannoni" group, a complex of several species in which fe ... | 1982 | 7072886 |
| influence of clinical presentation on the efficacy of a short course of pentamidine in the treatment of cutaneous leishmaniasis in french guiana. | the cure 'rates' achieved using intramuscular pentamidine isethionate (two injections of 4 mg/kg separated by an interval of 48 h) were investigated in french guiana, in 198 consecutive patients with cutaneous leishmaniasis caused by leishmania braziliensis guyanensis. one aim was to see if initial clinical presentation could be used to predict treatment failure. the cure rate after one course of pentamidine isethionate was 87% and almost all (80%) of the treatment failures responded to an ident ... | 2001 | 11454242 |
| the importance of edentates (sloths and anteaters) as primary reservoirs of leishmania braziliensis guyanensis, causative agent of "pianbois" in north brazil. | | 1981 | 7324143 |
| leishmania braziliensis guyanensis dermal leishmaniasis: cell-mediated immunity related to clinical features. | the non-specific and specific cellular immune responses in vitro were investigated in 26 patients infected with leishmania braziliensis guyanensis and compared with those of 12 healthy donors. an absence of marked suppression of lymphocyte proliferation in vitro to con a, pha and pwm mitogens, and a high variability in the cellular responsiveness to leishmania antigens were detected in the infected patient group. the occurrence of relapses seemed to be associated with a lower lymphocyte transfor ... | 1985 | 3887683 |
| [note on the relations between vectors of leishmaniasis and forest trees in french guiana]. | the study of relationships between phlebotomine sandflies and different species of trees, 0 to 1.5 m above ground, was carried out in the french guiana forest. we found that certain forest trees are more propitious than others to the development or maintenance of certain leishmaniasis vectors. lu. umbratilis, vector of leishmania braziliensis guyanensis, represents between 28 and 88% of samples collected on trunks. the association of lu. umbratilis and lu. rorotaensis represents between 58 and 9 ... | 1986 | 3813430 |
| lr1: a candidate rna virus of leishmania. | although viruses are important biological agents and useful molecular tools, little is known about the viruses of parasites. we report here the discovery of a candidate for an rna virus in a kinetoplastid parasite. this potential virus, which we term lr1, is present in the promastigote form of the human pathogen leishmania braziliensis guyanensis cumc1-1a but not in 11 other stocks of leishmania that were examined nor in trypanosoma brucei. the candidate viral rna has a size of approximately 600 ... | 1988 | 3200841 |
| epidemiology of leishmaniasis in french guiana. | lutzomyia umbratilis is confirmed as the vector of leishmania braziliensis guyanensis, the cause of "pian bois" in man in french guiana. although spending most of the year in the forest canopy, this sandfly is abundant at ground level for about two weeks at the beginning of the long rainy season. the maximum number of infective bites per man per hour (3.9) was reached at the end of november 1979. infections were found in one of seven potos flavus, seven of 15 choleopus didactylus and two of 19 r ... | 1982 | 7080142 |
| a pilot study to control lutzomyia umbratilis (diptera:psychodidae), the major vector of leishmania braziliensis guyanensis, in a peri-urban rainforest of manaus, amazonas state, brazil. | in the second half of 1980, 112 (or ca. 16%) of the inhabitants of the new settlement of são josé, city of manaus, contracted cutaneous leishmaniasis whilst clearing their properties of terra firme rainforest. with the aid of sucam, the authors carried out a pilot study to investigate the feasibility of reducing populations of lutzomyia umbratilis, the local silvatic vector of leishmania braziliensis guyanensis, by spraying insecticide on its favoured diurnal resting sites, the bases of the larg ... | 1985 | 4088044 |
| flagellate infections of brazilian sand flies (diptera: psychodidae): isolation in vitro and biochemical identification of endotrypanum and leishmania. | flagellate infections were found in 1,063 of 18,895 sand flies collected in the states of amazonas, pará, rondonia and acre, brazil. infection rates were 13.4% (species group shannoni); 7.5% (subgenus nyssomyia); 6.7% (subgenus lutzomyia series cruciata); 0.5% (genus psychodopygus) and 3.1% for other sand flies (various subgenera). leishmania braziliensis guyanensis and l. mexicana amazonensis were isolated, respectively, from the known vectors, lutzomyia umbratilis and l. flaviscutellata. singl ... | 1985 | 3938924 |