| rapid single-tube immunocapture rt-pcr for the detection of two yam potyviruses. | an immunocapture reverse transcription-polymerase chain reaction (ic-rt-pcr) based assay has been developed for the detection of yam-infecting potyviruses. based upon the same format two distinct simple tests have been developed, which allow the reliable diagnosis of yam mosaic virus and the tentatively named yam mild mosaic virus. by using immunocapture and a single-buffer rt-pcr reaction, the test can be performed in a single tube. the tests described have been shown to exhibit a thousand-fold ... | 1997 | 9504753 |
| nucleotide sequence of coat protein gene of yam mild mosaic virus, isolated in papua new guinea. | we determined the 3'-termimus 1353 nucleotides (nts) in length excluding the poly (a) tail of yam mild mosaic potyvirus (ymmv) rna. the sequence starts within a long open reading frame (orf) 1209 nts and is followed by untranslated region (3'-utr) of 144 nts. the coat protein (cp) contains 266 amino acids (aa) with molecular ratio (mr) of approximately 30 kda. the cp of ymmv differs substantially from yam mosaic virus (ymv), japanese yam mosaic virus (jymv) (57 and 61% of amino acid sequence ide ... | 1999 | 10481747 |
| origin, world-wide dispersion, bio-geographical diversification, radiation and recombination: an evolutionary history of yam mild mosaic virus (ymmv). | we developed an evolutionary epidemiological approach to understand the regional and world-wide dispersion of yam mild mosaic virus (ymmv) by retracing its evolutionary history. analyses of the distribution and the prevalence of ymmv in the caribbean islands of guadeloupe and martinique, and in french guyana revealed that ymmv has a wide repartition and different prevalence on dioscorea alata l. (asian and oceanic origin), on d. cayenensis lam.-d. rotundata poir. (african origin) and on d. trifi ... | 2003 | 14522183 |
| daphne mosaic virus (dapmv), a new potyvirus from daphne mezereum in the czech republic. | daphne shrubs with light green rings and mosaic on leaves contained flexuous filamentous virions (696 x 13 nm) and cylindrical inclusions typical of the subdivision iii of edwardson's classification for inclusions induced by members of the family potyviridae. decoration tests using antisera to 67 potyviruses revealed distant serological relations among chilli veinal mottle virus, colombian datura virus, papaya ringspot virus, tobacco vein mottling virus and yam mosaic virus. the 3' terminal regi ... | 2006 | 16292598 |
| the complete genome sequence of a brazilian isolate of yam mild mosaic virus. | in this study, the complete genome of an isolate of yam mild mosaic virus (ymmv) from brazil was sequenced, and the predicted amino acid sequence was analyzed. the ymmv rna genome consists of 9538 nt without the poly(a) tail, encoding a putative typical potyvirus polyprotein of 3084 amino acids. furthermore, the small overlapping orf (pipo) in the p3 gene was also deduced, and the cleavage sites of the polyprotein were predicted. multiple alignment with other potyviruses showed a maximum nucleot ... | 2013 | 23081678 |