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[herpesvirus epidemic in greek (testudo hermanni) and moorish land tortoises (testudo graeca) in switzerland].based on an epidemic in switzerland, the histopathological findings of a viremic herpes infection in spur-tailed and spur-thighed mediterranean land tortoises are described for the first time. horizontal viral transmission has been proved; the vertical transmission is suggested. an impression smear of the tongue enables an early clinical diagnosis.19902163101
influence of incubation temperaure on interferon mechanism in cells of testudo graeca kidney. 19734148898
cultivating the virus of the variolovaccine in tissue culture of pancreas of testudo graeca. 19664288456
multiplication of myxovirus parainfluenzae 1 (sendai) in lung tissue culture of tortoise (testudo graeca). 19694319709
[experimental infection of tortoises of the species testudo graeca and testudo hermanni with salmonella bacteria]. 19664862033
[effects of an estrogen hormone, estradiol benzoate, on the moorish turtle (testudo graeca l.) embryo urogenital apparatus]. 19694983264
[on the length of time of fecal arizona elimination in land turtles of the species testudo graeca and testudo hermanni]. 19655857510
[distribution of arizona bacteria among free-living land turtles of the species testudo graeca and testuda hermanni]. 19655857511
[on the duration of fecal elimination of salmonellae in land turtles of the orders testudo graeca and testudo hermanii]. 19655869208
interferon production in arbovirus-infected cell cultures of tortoise (testudo graeca) kidney. 19734405393
pneumonia in moorish tortoises (testudo graeca) associated with avian serovar a chlamydia psittaci. 19947817509
detection and isolation of a herpesvirus from a spur-thighed tortoise (testudo graeca) in the uk. 199910606021
diseases of the respiratory tract of chelonians.diseases of the respiratory tract commonly occur in captive chelonians, and several diseases also have occurred in wild chelonians. infectious causes include viruses, bacteria, fungi, and parasites. herpesviruses have surfaced as important pathogens of the oral cavity and respiratory tract in hermann's tortoise (testudo hermanii), spur-thighed tortoise (testudo graeca), and other tortoises in europe and the united states. herpesvirus-associated respiratory diseases also have been reported in the ...200011228895
enzyme-linked immunosorbent assay for detecting herpesvirus exposure in mediterranean tortoises (spur-thighed tortoise [testudo graeca] and hermann's tortoise [testudo hermanni]).an enzyme-linked immunosorbent assay (elisa) was developed for the detection of antibodies to a herpesvirus associated with an upper respiratory tract disease in mediterranean tortoises [spur-thighed tortoise (testudo graeca) and hermann's tortoise (testudo hermanni)]. this serodiagnostic test was validated through a hyperimmunization study. the mean of the a(405) readings of the plasma samples collected at time zero of the hyperimmunization study plus three times the standard deviation was used ...200111526144
[salmonella in tortoises (testudo graeca) in bulgaria. i]. 196113886458
intercellular development of mycobacterium tuberculosis (typus humanus) in tissue culture of kidney epithelium of tortoise (testudo graeca). 196213992760
[multiplication of mumps virus, vaccinia virus and herpesvirus on tortoise (testudo graeca) kidney cells cultured by a simplified method]. 196314048735
[eosinophilic nuclear inclusions induced by the multiplication of newcastle disease virus in tortoise (testudo graeca) kidney cells]. 196314048736
[cultivation of the virus of vesicular stomatitis on tissue culture of cold-blooded animals(testudo graeca)]. 196414219378
the resistance of tortoises, testudo graeca l., against toxocara canis infection. 196514272967
mortality of mediterranean tortoises (testudo graeca and testudo hermanni) in pet shops prior to sale. 19873427394
cytotoxic effects of some animal and vegetable extracts and some chemicals on liver and colon carcinoma and myosarcoma.to study, the cytotoxic effects of some biological and chemical agents on g1, s, g2, m and g0 phases of liver and colon carcinomas and myosarcoma cells obtained with chemical carcinogens dimethylbenzanthracene (dmba) and cadmium chloride.200414968209
[on the characteristics of fecal salmonella carrier state in the land turtle of the species testudo graeca and testudo hermanni]. 19665994746
[rapd variation in mediterranean turtle testudo graeca l. (testudinidae)].the polymerase chain reaction with arbitrary primers (rapd-pcr) was used to study intraspecific variation in mediterranean turtle testudo graeca, which is represented by the dagestan (t. g. pallasi) and nikolskii (t. g. nikolskii) subspecies in russia. to study the phylogenetic relationships, the rapd variation was also compared in two other t. graeca subspecies (t. g. ibera and t. g. terrestris), two closely related testudo species (t. kleinmanni and t. marginata), and central asian turtle agri ...200415648145
mitochondrial haplotype diversity in the tortoise species testudo graeca from north africa and the middle east.to help conservation programs of the endangered spur-thighed tortoise and to gain better insight into its systematics, genetic variation and evolution in the tortoise species testudo graeca (testudines: testudinidae) was investigated by sequence analysis of a 394-nucleotide fragment of the mitochondrial 12s rrna gene for 158 tortoise specimens belonging to the subspecies testudo graeca graeca, testudo graeca ibera, testudo graeca terrestris, and a newly recognized subspecies testudo graeca white ...200515836787
[distribution of fecal salmonella and arizona carriers in the free living turtles testudo graeca and testudo hermanni]. 19665991750
antibodies to viruses in wild-caught spur-thighed tortoises (testudo graeca) in turkey. 200717652436
cryptosporidium from tortoises: genetic characterisation, phylogeny and zoonotic implications.cryptosporidiosis is a disease caused by apicomplexan parasites of the genus cryptosporidium, which affect the gastrointestinal tract of humans and animals worldwide. in the present study, cryptosporidium isolates from captive european tortoises (testudo graeca, testudo hermanni and testudo marginata) in italy were genetically characterised using gene/s encoding cryptosporidium oocyst wall protein (cowp) and/or the hypervariable segment internal to the ssu rdna. six isolates from t. graeca, t. h ...200818234467
co-distribution pattern of a haemogregarine hemolivia mauritanica (apicomplexa: haemogregarinidae) and its vector hyalomma aegyptium (metastigmata: ixodidae).hyalomma aegyptium ticks were collected from tortoises, testudo graeca, at localities in northern africa, the balkans, and the near and middle east. the intensity of infestation ranged from 1-37 ticks per tortoise. the sex ratio of feeding ticks was male-biased in all tested populations. larger tortoises carried more ticks than did the smaller tortoises. the juveniles were either not infested, or carried only a poor tick load. hyalomma aegyptium was absent in the western souss valley and ourika ...200918954156
hemolivia mauritanica (haemogregarinidae: apicomplexa) infection in the tortoise testudo graeca in the near east with data on sporogonous development in the tick vector hyalomna aegyptium.testudo groeca tortoises were collected in the northern and southern golan heights (israeli occupied territory of south syria), and various locations in israel and palestine. hyoalomma oegyptium ticks were found only on golan height tortoises, and only the tortoises and ticks from the northern golan heights were infected with hemolivia mouritanica. tortoises became infected after ingesting infected ticks. male ticks carrying sporocysts, which remain attached to tortoises for extended durations, ...200617285846
salmonella in free living terrestrial and aquatic turtles.detection of salmonella in pet turtles has been the focus of extensive research, but its incidence in free living turtles is not well known. the aim of this study was to evaluate the incidence of salmonella in terrestrial and aquatic species of chelonians inhabiting a national park in southwestern spain. individuals of the terrestrial tortoise testudo graeca (n = 16) and the aquatic turtles emys orbicularis (n = 26) and mauremys leprosa (n = 50) were investigated. maximum incidence of salmonella ...200716979850
epidemiology and biology of nematodofauna affecting testudo hermanni, testudo graeca and testudo marginata in italy.tortoises of the genus testudo living in italy are testudo hermanni, testudo graeca and testudo marginata. although a great deal of information has been acquired on the internal medicine and surgery on these animals, little is known of their parasitological fauna. a survey on the presence of gastro-intestinal parasitic nematodes in tortoises bred in italy was carried out to acquire extensive epidemiological data. stool samples of 62 tortoises (37 t. hermanni hermanni, 13 t. graeca, 6 t. hermanni ...200516237575
granulomatous hepatitis caused by salmonella typhimurium in a spur-thighed tortoise (testudo graeca). 200516113171
prevalence of mycoplasma agassizii and chelonian herpesvirus in captive tortoises (testudo sp.) in the united kingdom.during the months of april to august in 1999 and 2002, oral swabs were collected from 146 tortoises (testudo sp.) in private collections in the united kingdom and tested by polymerase chain reaction (pcr) for the presence of mycoplasma agassizii and chelonian herpesvirus (chhv). the presence of m. agassizii was confirmed by restriction digestion of the pcr product. a 307-bp fragment of the chhv ul5 homologue gene was sequenced and found to show most similarity to equine herpesvirus type 1. a pre ...200415193070
zoonotic pathogens associated with hyalomma aegyptium in endangered tortoises: evidence for host-switching behaviour in ticks?hyalomma aegyptium is a hard-tick with a typical three-host life cycle. the main hosts are palearctic tortoises of genus testudo. however, other hosts can be used by immature ticks for feeding in natural conditions. given this complex ecology and multiple host use, the circulation of pathogens by h. aegyptium between various hosts can be important from epidemiological point of view. the aim of this study was to evaluate the role of h. aegyptium as natural carrier of four important zoonotic patho ...201223273169
experimental transmission of a herpesvirus in greek tortoises (testudo graeca).an experimental transmission study aimed at fulfilling koch's postulates for a herpesvirus-associated stomatitis-rhinitis in mediterranean tortoises is presented. clinical, pathologic, serologic, and molecular studies were performed linking tortoise herpesvirus with the pathogenesis of stomatitis-rhinitis. four adult greek tortoises received either intranasally or intramuscularly two tortoise herpesvirus isolates by primary experimental infection and secondary challenge 11 months later. after th ...200414715968
[influence of the serum and plasma of testudo graeca on the culture of embryonal hepatic tissue and human amnion]. 196114483041
gonadogenesis in embryos of two tortoise species, emys orbicularis l. and testudo graeca l., at different temperatures. 20164778513
[the presence in the serum of the bulgarian turtle testudo graeca of agglutinating pasteurella tularensis]. 196113733589
pulmonary candidiasis caused by candida albicans in a greek tortoise (testudo graeca) and treatment with intrapulmonary amphotericin b.an adult female greek tortoise (testudo graeca) presented with dyspnea, lethargy, and anorexia. severe unilateral pulmonary candidiasis was diagnosed and confirmed by histologic and microbiologic evaluations. initial treatment with ketoconazole resulted in plasma elevations of aspartate aminotransferase, lactate dehydrogenase, and bile acids consistent with imidazole-induced hepatotoxicity. plasma chemistry abnormalities resolved upon withdrawal of the drug. temporary osteotomy permitted access ...200112785685
redescription of mehdiella microstoma and description of mehdiella petterae sp. n., with a new definition of the genus mehdiella seurat, 1918 (nematoda: pharyngodonidae).the generic diagnosis of mehdiella seurat, 1918 is emended based on study and redescription of mehdiella microstoma (drasche, 1884) from the caecum of testudo graeca linnaeus, 1758 collected in settat, morocco and on study and description of a new species, mehdiella petterae sp. n., from the large intestine of testudo hermanni (gmelin, 1789) collected in catalonia, spain. scanning electron microscopy (sem) studies revealed substantial differences in the structure of the mouth and the caudal end, ...200111437127
oxyuroids of palearctic testudinidae--new definition for alaeuris seurat, 1918 (nematoda: pharyngodonidae) and redescription of alaeuris numidica (seurat, 1918).the generic diagnosis of alaeuris is emended based on the study and redescription of alaeuris numidica from the cecum of testudo graeca collected in settat, morocco and of testudo hermanni collected in catalonia, spain. scanning electron microscopy studies revealed that the papillae previously described as adanal are simple lobes because of the lack of terminal nerves, and that both preanal and postanal papillae are pedunculate. these new findings allowed us to emend the diagnosis of the genus a ...200111227877
chronic rhinitis associated with herpesviral infection in captive spur-thighed tortoises from spain.an epidemic of chronic rhinitis in a population of 50 captive spur-thighed tortoises (testudo graeca graeca) from palafrugell (girona, spain) is described, in which eight animals died and 12 were euthanatized to perform necropsies and post-mortem studies. the main clinical sign was a bilateral, seromucous rhinitis often accompanied by stomatitis and glossitis. hematology and serum biochemistry were performed in 33 of the 50 ill animals and in 29 healthy tortoises from three disease-free populati ...19989706558
prevalence of hemolivia mauritanica (apicomplexa: adeleina: haemogregarinidae) in natural populations of tortoises of the genus testudo in the east mediterranean region.hemolivia mauritanica (sergent et sergent, 1904) infections were found in 14% (n= 14) of testudo graeca linnaeus, 1758 tortoises in bulgaria and in 92% (n = 26) of those in turkey. hemolivia mauritanica-like gametocytes were found in 81% (n = 47) of wild testudo marginata schoepff, 1792 tortoises in greece. parasitaemia intensity (the percentage of infected red blood cells found in approximately 104 cells) was 0.03-22.4% in t. graeca and 0.06-12.27% in t. marginata. on the other hand, all blood ...200516405300
herpesviruses in tortoises: investigations into virus isolation and the treatment of viral stomatitis in testudo hermanni and t. graeca.various studies were done during a spontaneous outbreak of stomatitis-rhinitis-complex (mouth rot) in a collection of mediterranean land tortoises (21 testudo hermanni, hermann's tortoises, and three testudo graeca, spur-thighed tortoises) in southern germany. these studies were intended to help diagnose the causative agent, establish a possible diagnostic method in vivo and provide information on the efficacy of aciclovir and ganciclovir against chelonian herpesviruses. thirteen t. hermanni and ...19979323927
cultivating the virus of abortion in sheep on tissue culture of cold-blooded animal (testudo graeca). 196414246385
[cultivation of rickettsia rickettsi (type dermacentroxenus conori) in tissue cultures of kidney from a cold-blooded animal (testudo graeca)]. 19675628417
[multiplication of respiratory-syncytial-virus in lung cell cultures of tortoises of the species testudo graeca]. 19695358862
rhinitis in long term captive mediterranean tortoises (testudo graeca and t hermanii).rhinitis is a common condition of captive mediterranean tortoises (testudo species). the interpretation of the results of the bacteriological examination of naso-oropharyngeal swabs from affected individuals has proved difficult, because the normal bacterial population of this region was unknown. no substantial differences in the strains of bacteria isolated from healthy individuals and from those affected by rhinitis could be found in this study. the report that sendai virus was implicated in t ...19854095882
borrelia turcica sp. nov., isolated from the hard tick hyalomma aegyptium in turkey.previously, a novel, fast-growing spirochaete was isolated from the hard tick hyalomma aegyptium, which infests tortoises (testudo graeca), by using barbour-stoenner-kelly (bsk) ii medium; the tick samples were taken from the istanbul area in northwestern turkey [güner et al. (2003). microbiology 149, 2539-2544]. here is presented a detailed characterization of the spirochaete. electron microscopy revealed that strain ist7t is morphologically similar to other spirochaetes of the genus borrelia a ...200415388724
[comparative anatomical study of the synovial membrane in representatives of 4 classes of terrestrial vertebrate].the synovial membrane (sm) of some representatives of four terrestrial vertebrate classes was studied by means of scanning electron microscope (sem). the figures of sm of the mammals (man, mini-pig), birds (fowl, gallus domesticus l.), reptiles (lizard, lacerta viridis laur. and turtle, testudo graeca l.) and amphibians (frog, rana esculanta l.) are documented. the sm of all animal species investigated presents a great locally variable appearance in form and surface arrangement of the living cel ...19873314799
isolation and partial characterization of igg-like immunoglobulins from frog (rana ridibunda pall.) and tortoise (testudo graeca pall.) serum.1. by successive application of gel-filtration and ion-exchange chromatography igg fraction have been isolated from frog (rana ridibunda pall.) and tortoise (testudo graeca pall.) sera. 2. the homogeneity of the fractions has been confirmed and a similar electrophoretic mobility has been revealed by polyacrylamide gel electrophoresis and immunoelectrophoresis. 3. in rabbits polyclonal antibodies giving specific reaction with the corresponding igg immunoglobulin have been raised.19883132347
comparison of total calcium, ionised calcium and albumin concentrations in the plasma of captive tortoises (testudo species).plasma total calcium, ionised calcium and albumin levels in hermann's tortoises (testudo hermanni), spur-thighed tortoises (testudo graeca), marginated tortoises (testudo marginata) and horsfield's tortoises (testudo horsfieldi) were investigated. the animals were captive adults maintained under natural unfiltered sunlight in the uk with no dietary sources of cholecalciferol. analysis of total calcium data showed statistically significant seasonal elevations in females in august compared with ma ...200919850853
detection and partial characterization of herpesviruses from egyptian tortoises (testudo kleinmanni) imported into italy from libya.a group of approximately 370 egyptian tortoises (testudo kleinmanni) and 36 spur-thighed tortoises (testudo graeca) were illegally imported into italy from libya. within 6 mo of their entry into italy, all but 40 of the egyptian tortoises had died with signs of severe stomatitis. herpesviruses were detected from the tongues of seven egyptian tortoises by polymerase chain reaction (pcr) and virus isolation. sequencing of a portion of the ul39 homologue of the herpesviruses from three different to ...200919368266
cultivation of sheep abortion virus in tissue culture of tortoise lung (testudo graeca). 19665951005
mortality in imported tortoises (testudo graeca and t. hermanni) in the united kingdom. 19883382907
considerations on the ependyma of the encephalic ventricles of tropidonotus natrix, alligator mississipiensis and testudo graeca. 19705492413
salmonella species in free-living spur-thighed tortoises (testudo graeca) in central western morocco. 200818281632
[sex ratio in the embryos of 2 chelonians (testudo graeca l. and emys orbicularis l.) born of artificially incubated ova]. 19714997326
blood delta-alad, lead and cadmium concentrations in spur-thighed tortoises (testudo graeca) from southeastern spain and northern africa.mediterranean spur-thighed tortoise (testudo graeca) is actually included in the iucn as vulnerable species. its main european population is located in southeastern spain. although a great deal of information has been acquired on the internal medicine and survey and even parasitological fauna on these animals, there are no references about contaminants levels in this species. the objectives of this study were to compare the levels of two metals (cadmium and lead) in the blood of spur-thighed tor ...201019937111
salmonella infection in illegally imported spur-thighed tortoises (testudo graeca).the prevalence of salmonella infection was determined in a group of spur-thighed tortoises (testudo graeca) seized during two smuggling attempts and in a population of captive hermann's tortoises (testudo hermanni) sheltered in a wildlife rescue centre. salmonella spp. was isolated in 81 of 220 (36.8%) and in 17 of 67 (25.4%) cloacal swabs collected from the t. graeca and t. hermanni tortoises respectively. overall, a total of 21 different salmonella serotypes were found. some of these serotypes ...201120626717
mycoplasma and herpesvirus pcr detection in tortoises with rhinitis-stomatitis complex in spain.chelonid herpesvirus (chhv) and mycoplasmal infections cause similar clinical signs in terrestrial tortoises and may be the most important causative agents of rhinitis-stomatitis complex, a common disease in captive tortoises worldwide. currently, diagnosis of chhv and mycoplasma spp. infections is most often based on serologic testing. however, serologic results only detect past exposure, and the specificity of these tests can be reduced due to antigenic cross-reactions with other pathogens. mo ...201121270008
detection and characterization of mycoplasma spp. and salmonella spp. in free-living european tortoises (testudo hermanni, testudo graeca, and testudo marginata).free-living and captive chelonians might suffer from upper respiratory tract disease (urtd), a pathology primarily caused by mycoplasma agassizii. wild tortoises can also be an important reservoir of salmonella spp., which are commensal in the host reptile but are potential zoonotic agents. between july 2009 and june 2010, we screened free-living european tortoises (spur-thighed tortoises testudo graeca, hermann's tortoises testudo hermanni, marginated tortoises testudo marginata) temporarily ho ...201121719840
the liver of testudo graeca (chelonia). a comparative study of hibernating and non-hibernating animals.in the present work, a comparative study of the liver of hibernating and non-hibernating specimens of testudo graeca (chelonia) has been carried out by light and electron microscopy. during hibernation, the morphological changes which take place do not affect the general architecture of the liver, but are mainly concerned with the number and distribution of the cytoplasmic organelles and reserve materials in the hepatocytes. in non-hibernating animals, the cytoplasm of the hepatocytes has a larg ...20133599126
ultrastructure of the respiratory epithelium in the lungs of the tortoise, testudo graeca.the respiratory epithelium in the lungs of the tortoise (testudo graeca) has been studied by electron microscopy. the epithelium consists of a mosaic of two different cell types (here called "pneumonocytes"). type i pneumonocytes are roughly squamous and possess attenuated flanges of cytoplasm which extend over the septal capillaries. localized cytoplasmic expansions are often present near the periphery of these flanges. most of the organelles are concentrated in the perinuclear region; the most ...1992195734
the distal forelimb musculature in aquatic and terrestrial turtles: phylogeny or environmental constraints?we compared the muscular anatomy of the distal front limb in terrestrial and aquatic chelonians to test whether observed differences between the two groups are associated with their divergent lifestyles and locomotor modes. given the different use of the forelimb in the two environments (body support and propulsion on land vs. mainly propulsion in water) we expected that: (1) aquatic and terrestrial turtles would show differences in their muscular anatomy, with aquatic species having more indivi ...200819172731
transstadial transmission of borrelia turcica in hyalomma aegyptium ticks.borrelia turcica comprises the third major group of arthropod-transmitted borreliae and is phylogenetically divergent from other borrelia groups. the novel group of borrelia was initially isolated from hyalomma aegyptium ticks in turkey and it was recently found in blood and multiple organs of tortoises exported from jordan to japan. however, the ecology of these spirochetes and their development in ticks or the vertebrate hosts were not investigated in detail; our aims were to isolate the patho ...201525695663
[synthesis activities in the apical epiblastic crest of forelimb anlagen in turtle (testudo graeca and emys orbicularis l.) embryos; histological and autoradiographic study]. 19724622080
myoid cells in the peritubular tissue (lamina propria) of the reptilian testis.the arrangement and fine structure of peritubular myoid cells was studied in the testes of three species of reptiles (lacerta dugesi, testudo graeca and natrix natrix) during two short periods of the seasonal cycle (european spring and autumn) and correlated with some ultrastructural properties of leydig cells. the lamina propria consists of myoid cells, fibroblasts and non-cellular components comprising collageneous and non-striated microfibrils. both components are arranged in alternating laye ...20051201592
a new species of haemoproteus from a tortoise (testudo graeca) in turkey, with remarks on molecular phylogenetic and morphological analysis.haemoproteus anatolicum n. sp. was identified in the tortoise testudo graeca. the new species is described based on the morphology of its blood stages and a segment of the mitochondrial cytochrome b gene, which can be used for molecular identification and diagnosis. chelonian haemoproteids recorded in the past were defined solely on the basis of their morphological characteristics. the chelonian haemoproteid we describe as a new species has a close genetic relationship to lizard haemoproteids, i ...201322924909
salmonella species in turtles.salmonella spp. occur abundantly in the feces of the turtles testudo graeca and t. hermanni but rarely in other testudines. these organisms do not seem to produce any kind of infection in the turtles. one possible explanation for the infestation is the coprophagic habit of t. graeca and t. hermanni..196217732196
lymphoid aggregates in gonads of embryos, hatchlings, and young of turtles with temperature-dependent sex determination.cellular infiltrations forming lymphoid-like aggregates were previously observed in gonads of two turtle species exhibiting temperature-dependent sex determination (tsd): at hatching in chelydra serpentina; at and after hatching in emys orbicularis. we show here that such aggregates are also present in gonads of testudo graeca by the end of embryonic development, suggesting that their occurrence is general in turtles. since in c. serpentina, infiltrations were observed mainly in testes exhibitin ...200414743515
hidden threat of tortoise ticks: high prevalence of crimean-congo haemorrhagic fever virus in ticks hyalomma aegyptium in the middle east.it is the first time that crimean-congo haemorrhagic fever virus (cchfv), causing potentially lethal disease of humans, has been reported from the middle east region and from the tortoise tick hyalomma aegyptium from a tortoise host, whose epidemiological significance may have remained almost completely overlooked so far. we used rt-pcr to screen for 245 ticks collected from 38 testudo graeca tortoise individuals. results of our genetic screening provide unambiguous evidence of occurrence of cch ...201424618184
[contacts of the basal processes of the visual cone cells in the outer plexiform layer of the sensory membrane of the eyeball in turtles].in two tortoise (agrionemys horsfieldi gray, testudo graeca l.) and three turtle (emys orbicularis l., clemmys (mauremys) caspica gmel, tryonix sinensis wieg) species, contacts of basal processes (synaptic peduncles of the cone cells) have been studied electron microscopically. their ultrastructure and peculiarities in organization of specialized junctions between each other and with other neural elements are described. the interreceptory contacts revealed are considered to be of one of the gap ...19836661055
[tunisian reptiles. iii. distribution and need for protection of terrestrial and fresh water turtles].the distribution and habitats in tunisia of the tortoise testudo graeca graeca and of the two turtles emys orbicularis and mauremys caspica leprosa are investigated throughout the country. the present low level of population density is recent; its causes are precised. the protection, more specially for the two first species, must be very efficient.2006747452
aortic intimal thickenings in tortoises.the aorta and coronary arteries of two tortoise species, testudo graeca ibera and emys orbicularis are investigated. the elastic aorta of the first species showed minimal focal intimal thickenings while the muscular aorta of the second presented marked diffuse hyperplasia narrowing the caliber of the vessel. the author discusses this striking finding of the aortic structural differences between the two species investigated as well as the significance of such lesions for atherosclerosis developme ...2003594625
[topography and cytoarchitectonics of the main cortical zones of the turtle forebrain].the description of cytoarchitectonics and topography of the three principle cortical zones of the forebrain of emys orbicularis reveals that the cortical structures in the species are precisely distinguished with respect to their neuronal characteristics, density and the situation of cellular elements. emys orbicularis occupies an intermediate position among the species of the order chelonia described in the literature as regards the level of development of the forebrain cellular structures. emy ...1977597065
ultrastructure of the joint receptors of the tortoise (testudo graeca. emys orbicularis).the ultrastructure of the spray-like ramified encapsulated corpuscles with the primitive inner core from the joint capsules of the large limb joints of the tortoise (testudo graeca and emys orbicularis) was examined. each of the branches of the receptor consists of three components. through the middle of the receptor branche runs the nerve terminal, containing in the receptor matrix numerous mitochondria, tiny light vesicles and neurofilaments and neurotubules running in the axial way. the nerve ...19751234809
[temperature effects on the development of genital glands in the embryos of 2 chelonians, emys orbicularis l. and testudo graeca l]. 19724622404
[contributions to ontogenesis of the hypophysis of testudo graeca and emys orbicularis, with special consideration of its relations to prechordal plate, chorda and intestinal roof]. 195613468674
differences in helminth infections between captive and wild spur-thighed tortoises testudo graeca in southern spain: a potential risk of reintroductions of this species.although the spur-thighed tortoise, testudo graeca, is one of the most widely distributed species of tortoises, its natural populations are threatened through its whole range. particularly at south-eastern spain, the species is mainly threatened by habitat destruction and over-collection, given that this chelonian has been traditionally considered an appreciate pet. as south-eastern spanish wildlife recovery centers shelter hundreds of captive animals mainly coming from illegal trade or captive- ...201222440722
prevalence and intensity of blood apicomplexan infections in reptiles from romania.in order to evaluate prevalence and intensity of apicomplexan hemoparasites in free-ranging reptiles from romania, blood smears were collected from european pond turtles (emys orbicularis), sand lizards (lacerta agilis), and spur-thighed tortoises (testudo graeca). all three host species were positive for blood parasites, with prevalence of infected individuals between 60.71% and 100% and variable intensity. similarities and differences with other epidemiological data are discussed.200818283494
whole genome characterization of a chelonian orthoreovirus strain identifies significant genetic diversity and may classify reptile orthoreoviruses into distinct species.in this study we report the sequence and phylogenetic characterization of an orthoreovirus strain, ch1197/96, isolated from a spur-thighed tortoise (testudo graeca) on chicken embryo fibroblast cells. the 23,957 bp long genome sequence was obtained by combined use of semiconductor and capillary sequencing. although the genomic characterization showed that the virus was most similar to the bush viper reovirus strain, 47/02, and in phylogenies performed with all segments the two strains formed a m ...201626892635
isolation and partial characterization of igm-like immunoglobulins from the serum of carp (cyprinus carpio l.), frog (rana ridibunda pall.) and tortoise (testudo graeca pall.).1. igm-like immunoglobulin fractions have been isolated by combinations of precipitation procedures and gel-filtration from the serum of three vertebrate species, representative of the classes of pisces (cyprinus carpio l.), amphibia (rana ridibunda pall.) and reptilia (testudo graeca pall.). 2. the homogeneity of the isolated fractions has been confirmed electrophoretically and immunoelectrophoretically. 3. electrophoretic mobility of igm, similar for the three species studied has been observed ...19883132346
descriptions of two new species of the genus tachygonetria wedl, 1862 (nematoda-pharyngodonidae) and discussion of the relationships among the species of the genus.two species of pinworm, parasites of testudo graeca linneaus, 1758 from morocco, are described: tachygonetria poulini sp. nov. and tachygonetria settatensis sp. nov. light and scanning electron microscopy studies revealed morphological differences in the structure of the cephalic end in both sexes, as well as in the structure of the caudal end in the male, which enabled us to differentiate these species from others, and from each other. the relationships among the species of the genus tachygonet ...200312898227
description of tachygonetria combesi n. sp. and redescriptions of four species of tachygonetria wedl, 1862 (nematoda: pharyngodonidae), with a new diagnosis of the genus.the generic diagnosis of tachygonetria wedl, 1862 is modified based on the study and redescription of tachygonetria vivipara wedl, 1862 (collected from large intestine of uromastyx acanthinurus bell, from north africa) and t. dentata (drasche, 1883) (collected from large intestine of testudo graeca linnaeus in settat, morocco and t. hermanni gmelin in catalonia, spain). the following taxa were redescribed: tachygonetria conica (drasche, 1883) and t. robusta (drasche, 1883) (both from the large i ...200212386421
soft plastron, soft carapace with skeletal abnormality in juvenile tortoises. histopathology and isolation of a novel picornavirus from testudo graeca and geochelone elegans.a disease is described in juvenile tortoises (testudo graeca and geochelone elegans) consisting mainly of a soft carapace, soft plastron and deformed skeleton. the aim of this study was to determine histopathological lesions and the biological properties of the isolated viruses.201425323213
surfing in tortoises? empirical signs of genetic structuring owing to range expansion.much of our current knowledge about the genetic dynamics in range expansions originates from models, simulations and microcosm experiments that need to be corroborated by field data. here, we report a neutral genetic pattern that matches the predictions of the genetic surfing theory. genetic surfing occurs when repeated founding events and genetic drift act on the wave of advance of an expanding population, promoting strong spatial structure. in the range expansion of the tortoise testudo graeca ...201323554278
physical reparative treatment in reptiles.the tissue growth necessary to achieve a complete or partial restitution ad integrum as a result of injury to soft tissue and/or hard times in reptiles is variable and often needs long time in relation to the species, to the habitat and to their intrinsic physiological characteristics. the purpose of this work was to see if the tissue optimization (to) treatment with radio electric asymmetric conveyer (reac) provided good results in these animals and whether its use translates into reduced time ...201323442770
multiple congenital malformations in a dicephalic spur-thighed tortoise (testudo graeca ibera).a 22-day-old dicephalic spur-thighed tortoise (testudo graeca ibera) died following a history of lethargy, anorexia and absence of defecation. the two heads were anatomically similar with independent reaction to external stimuli. the carapace showed doubled first and extra second vertebral scutes. radiography and transplastronal ultrasonography, performed when the animal was alive, revealed two symmetrical stomachs and two asynchronous hearts. these findings were confirmed by necropsy examinatio ...201323352900
first records of hyalomma aegyptium (acari: ixodida: ixodidae) from the russian spur-thighed tortoise, testudo graeca nikolskii, with an analysis of tick population dynamics.during the fall of 1995 and the spring of 1996, 77 statistically comparable tick collections, comprising 792 specimens, were made from adults of the russian spur-thighed tortoise, testudo graeca nikolskii, at 4 sites along russia's black sea coast. these are the first tick collections reported from t. g. nikolskii since its recognition as a taxonomic entity. all ticks were determined to be hyalomma (hyalomma) aegyptium, a common tortoise parasite in southern russia that in 1930 was erroneously d ...19989920339
[autoradiographic study of the anterior limb-bud development in two species of chelonians (testudo graeca l. and emys orbicularis l)]. 19734717984
human-mediated secondary contact of two tortoise lineages results in sex-biased introgression.human-mediated secondary contact of recently diverged taxa offers valuable opportunities for studying the evolutionary mechanisms involved in the establishment and maintenance of genetic boundaries between taxa. we used mitochondrial and microsatellite markers to examine a recently introduced population of the spur-thighed tortoise (testudo graeca) of mixed origin in the doñana national park (sw spain). the earliest records of tortoises in doñana trace back to the 18th century, but several popul ...201728642457
hyperplastic stomatitis and esophagitis in a tortoise (testudo graeca) associated with an adenovirus infection.a 2-year-old female, spur-thighed tortoise (testudo graeca) was presented with poor body condition (1/5) and weakness. fecal analysis revealed large numbers of oxyurid-like eggs, and radiographs were compatible with gastrointestinal obstruction. despite supportive medical treatment, the animal died. at gross examination, an intestinal obstruction was confirmed. histopathology revealed severe hyperplastic esophagitis and stomatitis with marked epithelial cytomegaly and enormous basophilic intranu ...201627486139
evaluation of local ecological knowledge as a method for collecting extensive data on animal abundance.the use of local ecological knowledge (lek) has been advocated for biodiversity monitoring and management. to date, however, it has been underused in studying wild populations of animals and, particularly, in obtaining quantitative abundance estimates. we evaluated lek as a tool for collecting extensive data on local animal abundance and population trends. we interviewed shepherds in southeastern spain, asking them to estimate the local abundance of the terrestrial tortoise testudo graeca. we qu ...200919183211
development of a consensus reverse transcription pcr assay for the specific detection of tortoise picornaviruses.picornaviruses (pvs) of different terrestrial tortoise species, previously designated as virus "x," have been frequently detected from various tissues by virus isolation in terrapene heart cell culture as the preferred laboratory method for diagnosis. here, we describe the development of 2 diagnostic reverse transcription (rt)-pcr-based assays for the identification and characterization of tortoise pvs belonging to the tentative genus topivirus to test the novel diagnostic systems, pvs were isol ...201627034342
wildlife dna forensics against crime: resolution of a case of tortoise theft.a paternity test was used to investigate a robbery case involving captive individuals of greek tortoise (testudo graeca). six tortoises were allegedly stolen from a private breeder and offered for sale on the web by the supposed thief. the stolen tortoises were confiscated by the rangers of the state forestry corps (cfs). a panel of 14 autosomal microsatellite loci was used to genotype the seized tortoises and ten individuals assumed to be legally owned by the breeder. kinship analyses reliably ...201424315609
the use of negative pressure wound therapy following subcarapacial abscess excision in a tortoise.a 42-year-old female testudo graeca was referred with a five-month history of a non-healing carapacial wound. previous treatment had involved long-term antibiotic therapy and conscious debridement of necrotic tissue. on presentation an extensive deep wound with purulent discharge was visible on the right side of the carapace. a computed tomographic scan was performed to assess the extent of the wound. this revealed a large carapacial deficit with two underlying soft tissue masses. surgical debri ...201323889043
gastrointestinal parasitic burdens in uk tortoises: a survey of tortoise owners and potential risk factors.despite gastrointestinal parasites being commonly diagnosed in captive tortoises throughout the uk, there is a lack of data regarding the prevalence. the aims of this study were to investigate the prevalence of gastrointestinal parasites in tortoises in the uk, and to investigate the factors affecting the prevalence of these parasites. owners were invited to submit a faecal sample from their tortoise in conjunction with a completed questionnaire covering details of signalment and husbandry. data ...201324158320
ultrasonographic diagnosis of pericardial effusion and atrial dilation in a spur-thighed tortoise (testudo graeca).ultrasonography was used to diagnose pericardial effusion, atrial dilatation and liver masses in a spur-thighed tortoise which was more than 80 years old and suffering from posthibernation anorexia, lethargy, oedema and pneumonia. the tortoise was treated twice with frusemide and ceftazidime for the pneumonia, resulting each time in a temporary remission for about a month. after a further recurrence, the animal was euthanased and the lesions predicted by ultrasound were confirmed postmortem. it ...200010718590
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