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isolation and biodiversity of hitherto undescribed soil bacteria related to bacillus niacini.the hitherto largely not described phylogenetic neighborhood of bacillus niacini has been explored by a comprehensive cultivation experiment and genomic variety studies. previous culture-independent studies demonstrated that approximately 15% of all bacillus 16s rdna directly extracted from soils worldwide was affiliated to b. niacini. seven different media were inoculated with soil suspensions in serial dilutions and incubated at different temperatures. then, bacterial colonies were picked and ...200415037965
ornithinibacillus gen. nov., with the species ornithinibacillus bavariensis sp. nov. and ornithinibacillus californiensis sp. nov.a gram-positive, aerobic, rod-shaped, motile, endospore-forming bacterium was isolated from pasteurized milk from bavaria, germany. 16s rrna gene sequence similarities indicated that strain wsbc 24001(t) was most closely related to virgibacillus species (95.3-96.1 %), oceanobacillus species (95.6-95.7 %), bacillus firmus iam 12464(t) (95.5 %) and bacillus niacini ifo 15566(t) (95.2 %). however, strain wsbc 24001(t) showed the highest level of sequence similarity to an unnamed strain, mb-9(t) (97 ...200616738118
bacillus pocheonensis sp. nov., a moderately halotolerant, aerobic bacterium isolated from soil of a ginseng field.a gram-positive, non-motile, endospore-forming bacterial strain, designated gsoil 420t, was isolated from soil of a ginseng field in pocheon province, south korea, and was characterized, using a polyphasic approach, in order to determine its taxonomic position. the novel isolate consisted of strictly aerobic, rod-shaped cells and was able to grow in medium supplemented with up to 12% nacl at 25 degrees c and ph 6.5-7.0. comparative 16s rrna gene sequence analysis showed that strain gsoil 420t fe ...200717978214
broad-range pcr, cloning and sequencing of the full 16s rrna gene for detection of bacterial dna in synovial fluid samples of tunisian patients with reactive and undifferentiated arthritis.broad-range rdna pcr provides an alternative, cultivation-independent approach for identifying bacterial dna in reactive and other form of arthritis. the aim of this study was to use broad-range rdna pcr targeting the 16s rrna gene in patients with reactive and other forms of arthritis and to screen for the presence of dna from any given bacterial species in synovial fluid (sf) samples.200919570210
microbial biofilms on the sandstone monuments of the angkor wat complex, cambodia.discoloring biofilms from cambodian temples angkor wat, preah khan, and the bayon and west prasat in angkor thom contained a microbial community dominated by coccoid cyanobacteria. molecular analysis identified chroococcidiopsis as major colonizer, but low similarity values (<95%) suggested a similar genus or species not present in the databases. in only two of the six sites sampled were filamentous cyanobacteria, microcoleus, leptolyngbya, and scytonema, found; the first two detected by sequenc ...201122006074
changes and recovery of soil bacterial communities influenced by biological soil disinfestation as compared with chloropicrin-treatment.soil bacterial composition, as influenced by biological soil disinfestation (bsd) associated with biomass incorporation was investigated to observe the effects of the treatment on the changes and recovery of the microbial community in a commercial greenhouse setting. chloropicrin (cp) was also used for soil disinfestation to compare with the effects of bsd. the fusarium wilt disease incidence of spinach cultivated in the bsd- and cp-treated plots was reduced as compared with that in the untreate ...201323958081
draft genome sequence of the nicotinate-metabolizing soil bacterium bacillus niacini dsm 2923.bacillus niacini is a member of a small yet diverse group of bacteria able to catabolize nicotinic acid. we report here the availability of a draft genome for b. niacini, which we will use to understand the evolution of its namesake phenotype, which appears to be unique among the species in its phylogenetic neighborhood.201425477409
bacillus ginsengisoli sp. nov., isolated from soil of a ginseng field.a novel bacterial strain dcy53(t) was isolated from a soil sample from a ginseng field and was characterized using a polyphasic approach. cells were gram-reaction-positive, rod-shaped, endospore-forming and motile with flagella. the strain was aerobic, catalase- and oxidase-positive, optimum growth temperature and ph were 30-37 °c and 6.0-7.5, respectively. on the basis of 16s rrna gene sequence analysis, strain dcy53(t) was shown to belong to the genus bacillus and the closest phylogenetic rela ...201322611202
isolation and identification of culturable bacteria from honeydew of whitefly, bemisia tabaci (g.) (hemiptera: aleyrodidae).bemisia tabaci (g.) is an important pest and a vector of gemini viruses infecting plants. during the process of feeding b. tabaci excretes honeydew which is rich in nutrients, and an excellent medium for microbial growth. recent report proved that volatile emitted by the honeydew associated bacteria of aphid, acyrthosiphon pisum harris was involved in natural enemy calling. thus understanding the honeydew associated bacteria is of paramount importance from the non-chemical method of insect pest ...201425606395
non contiguous-finished genome sequence and description of bacillus jeddahensis sp. nov.strain jce(t) was isolated from the fecal sample of a 24-year-old obese man living in jeddah, saudi arabia. it is an aerobic, gram-positive, rod-shaped bacterium. this strain exhibits a 16s rrna nucleotide sequence similarity of 97.5 % with bacillus niacini, the phylogenetically closest species with standing nomenclature. moreover, the strain jce(t) presents many phenotypic differences, when it is compared to other bacillus species, and shows a low maldi-tof mass spectrometry score that does not ...201526380635
hyaluronate lyase of a deep-sea bacillus niacini.a hyaluronate lyase (bnihl) was purified to homogeneity from a culture of a deep-sea bacillus niacin strain jam f8. the molecular mass of purified bnihl was approximately 120 kda. the purified enzyme degraded hyaluronan as well as chondroitin sulfates a and c by a β-elimination mechanism. the optimal ph and temperature were around ph 6 and 45 °c for hyaluronan degradation. the enzyme required optimally 2, 50, and 100 mm calcium ions for degradation of hyaluronan, chondroitin sulfate c, and chond ...201525680511
draft genome sequence of a deep-sea bacterium, bacillus niacini strain jam f8, involved in the degradation of glycosaminoglycans.here, we report the draft genome sequence of bacillus niacini jam f8, which was newly isolated from deep-sea sediment at a depth of 2,759 m from the izu-ogasawara trench. an array of genes related to degradation of glycosaminoglycans in this bacterium was identified by whole-genome analysis.201425278530
thermotolerant bacillus kokeshiiformis sp. nov. isolated from marine animal resources compost.a novel gram-staining-positive, endospore-forming, rod-shaped, facultatively anaerobic, thermotolerant bacterium, designated strain mo-04(t), was isolated from a marine animal resources (mar) compost. the 16s rrna gene sequence of strain mo-04(t) showed 99.4% similarity with bacillus thermolactis r-6488(t), 94.1% similarity with bacillus thermoamylovorans cncm i-1378(t), 93.3% similarity with bacillus humi lmg 22167(t), 93.2% similarity with bacillus niacini ifo 15566(t) and the similarities wit ...201424835163
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