| a numerical classification of the genus bacillus. | three hundred and sixty-eight strains of aerobic, endospore-forming bacteria which included type and reference cultures of bacillus and environmental isolates were studied. overall similarities of these strains for 118 unit characters were determined by the ssm, sj and dp coefficients and clustering achieved using the upgma algorithm. test error was within acceptable limits. six cluster-groups were defined at 70% ssm, which corresponded to 69% sp and 48-57% sj. groupings obtained with the three ... | 1988 | 3246588 |
| biological treatment of tannery wastewater by using salt-tolerant bacterial strains. | abstract: | 2008 | 18445252 |
| isolation and identification of an alkaliphilic bacillus flexus xju-3 and analysis of its alkaline amylase. | to isolate and identify a new bacillus strain capable of growing under highly alkaline conditions as alkaline amylase producers and to characterize its enzymatic properties. | 2008 | 18720839 |
| antibacterial activity and qsar of chalcones against biofilm-producing bacteria isolated from marine waters. | biofouling in the marine environment is a major problem. in this study, three marine organisms, namely bacillus flexus (ld1), pseudomonas fluorescens (md3) and vibrio natriegens (md6), were isolated from biofilms formed on polymer and metal surfaces immersed in ocean water. phylogenetic analysis of these three organisms indicated that they were good model systems for studying marine biofouling. the in vitro antifouling activity of 47 synthesized chalcone derivatives was investigated by estimatin ... | 2010 | 20544550 |
| isolation and characterization of new facultative alkaliphilic bacillus flexus strains from maize processing waste water (nejayote). | this work describes the isolation and characterization of two new alkaliphilic micro-organisms present in nejayote. | 2011 | 21299574 |
| naturally occurring culturable aerobic gut flora of adult phlebotomus papatasi, vector of leishmania major in the old world. | cutaneous leishmaniasis is a neglected, vector-borne parasitic disease and is responsible for persistent, often disfiguring lesions and other associated complications. leishmania, causing zoonotic cutaneous leishmaniasis (zcl) in the old world are mainly transmitted by the predominant sand fly vector, phlebotomus papatasi. to date, there is no efficient control measure or vaccine available for this widespread insect-borne infectious disease. | 2012 | 22629302 |
| antimicrobial resistance and plasmid profile of bacterial strains isolated from the urbanized eltsovka-1 river (russia). | antimicrobial resistance and plasmid profile of gram-positive and gram-negative bacterial strains isolated from the urbanized eltsovka-1 river (russia) were investigated. sequencing of the 16s rrna of of g+ strains showed 99-100% identity to that of bacillus aerophilus, bacillus altitudinis, bacillus amyloliquefaciens, bacillus anthrancis, bacillus barbaricus, bacillus cereus, bacillus flexus, bacillus indriensis, bacillus stratosphericus, bacillus subtilis subsp. subtilis, bacillus thuringiensi ... | 2015 | 25803299 |
| biodiversity of aerobic endospore-forming bacterial species occurring in yanyanku and ikpiru, fermented seeds of hibiscus sabdariffa used to produce food condiments in benin. | yanyanku and ikpiru made by the fermentation of malcavene bean (hibiscus sabdariffa) are used as functional additives for parkia biglobosa seed fermentations in benin. a total of 355 aerobic endospore-forming bacteria (aefb) isolated from yanyanku and ikpiru produced in northern and southern benin were identified using phenotypic and genotypic methods, including gtg5-pcr, m13-pcr, 16s rrna, gyra and gyrb gene sequencing. generally, the same 5-6 species of the genus bacillus predominated: bacillu ... | 2013 | 23571124 |
| antibiofilm activity of biosurfactant producing coral associated bacteria isolated from gulf of mannar. | coral associated bacteria (cab) (n = 22) isolated from the mucus of the coral acropora digitifera were screened for biosurfactants using classical screening methods; hemolysis test, lipase production, oil displacement, drop collapse test and emulsifying activity. six cab (u7, u9, u10, u13, u14, and u16) were found to produce biosurfactants and were identified by 16s ribosomal rna gene sequencing as providencia rettgeri, psychrobacter sp., bacillus flexus, bacillus anthracis, psychrobacter sp., a ... | 2014 | 25320434 |
| two-stage phyto-microremediation of tannery effluent by spirodela polyrrhiza (l.) schleid. and chromium resistant bacteria. | two-stage sequential treatment of tannery effluent was conducted employing a wetland plant, spirodela polyrrhiza (l.) schleid., and chromium (cr) resistant bacterial strains. the bacterial strains were isolated from cr-enriched environmental matrices and rhizosphere of spirodela polyrrhiza. the phyto-rhizoremediation of tannery effluent by spirodela and its rhizospheric bacteria (cellulomonas biazotea apbr1-6, bacillus safensis apbr2-12, staphylococcus warneri apbr3-5, microbacterium oleivorans ... | 2016 | 27318662 |
| biodegradation of 17β-estradiol by bacteria isolated from deep sea sediments in aerobic and anaerobic media. | endocrine disrupting compounds (edcs) are considered as high research priority being a source of potential adverse ecological health effects in environmental waters. 17β-estradiol (e2), a recalcitrant natural estrogen, is typically encountered in wastewater treatment plants (wwtps) at levels ranging 10-30ngl(-1) in the influent flow and 1-3ngl(-1) in the effluent flow. the exposure to even extremely low concentrations of e2 may interfere with the normal function of the endocrine system of organi ... | 2017 | 27233210 |
| isolation, fingerprinting and genetic identification of indigenous pahs degrading bacteria from oil-polluted soils. | in the present study, thirty five bacterial isolates were obtained from hydrocarbon-contaminated soil samples using an enrichment method. these isolates were tested to grow on mineral salt medium containing anthracene or phenanthrene as sole carbon source. only five isolates showed the ability to degrade these compounds. rapd-pcr fingerprinting was carried out for the five isolates, and the dna patterns revealed that there was no similarity among the examined bacteria whenever the rflp using fou ... | 2016 | 26930863 |
| bacillus flexus outbreak in a tertiary burn center. | | 2016 | 26822696 |
| isolation and molecular characterization of multidrug-resistant halophilic bacteria from shrimp farm effluents of parangipettai coastal waters. | multidrug resistance of heterotrophic bacteria isolated from an aquaculture farm effluent in parangipettai, at the southeastern coast of india, was investigated. in the initial screening, 27 antibiotic-resistant strains were isolated. all the strains were tested for antibiotic susceptibility against chloramphenicol with varying concentrations. from these, two highly resistant strains, i.e. s1 and s5, were isolated. the selected strains were identified by 16s ribosomal rna (rrna) sequencing techn ... | 2015 | 25850744 |
| identification of intestinal bacterial flora in rhipicephalus microplus ticks by conventional methods and pcr-dgge analysis. | in this study, we have analyzed the intestinal microbial flora associated with rhipicephalus microplus ticks using both culture-dependent and independent methods based on pcr and denaturing gradient gel electrophoresis (pcr-dgge). the r. microplus ticks were collected from cattle and goats in jiangxi, hunan and guizhou provinces of china. three distinct strains of bacteria were isolated using culture-dependent methods: staphylococcus simulans, bacillus subtilis and bacillus flexus strain. ninete ... | 2015 | 25784070 |
| synergistic growth of bacillus and pseudomonas and its degradation potential on pretreated polypropylene. | this study investigated biodegradation of physically pretreated polypropylene (pp) by using two different combinations of microorganisms, namely, bacillus flexus + pseudomonas azotoformans(b1) and b. flexus + b. subtilis(b2), for a period of 12 months. the growth rate of (b1) was found to be high throughout the study period, and reached a maximum of 1 × 10(14) colony-forming units (cfu)/ml. at the end of the experiment, the polymers become hydrophilic. carbonyl indices showed that ultraviolet (u ... | 2016 | 25551336 |
| bacillus paraflexus sp. nov., isolated from compost. | a gram-stain-positive, rod-shaped, endospore-forming, aerobic bacterium capable of growing at 15-42 °c (optimum 30 °c) and at ph 5-11 (optimum ph 7) was isolated from compost. its taxonomic position was deduced using a polyphasic approach and the strain was designated rc2(t). 16s rrna gene sequence analysis showed that the isolate belongs to the division firmicutes, forming a clade within the cluster containing bacillus flexus ifo 15715(t), and showed highest similarity to b. flexus ifo 15715(t) ... | 2013 | 23990650 |
| identification and discrimination of bacteria using fourier transform infrared spectroscopy. | bacterial spectra were obtained in the wavenumber range of 4000-600 cm(-1) using ftir spectroscopy. ftir spectral patterns were analyzed and matched with 16s-rrna signatures of bacterial strains os1 and os2, isolated from oil sludge. specific spectral bands obtained from os1 (fj226761), reference strain bacillus flexus (atcc 49095), os2 (fj215874) and reference strain stenotrophomonas maltophilia (atcc 19861) respectively, suggested that os1 and atcc 49095 were closely related whereas os2 was di ... | 2013 | 23973597 |
| cellular fatty acid profile and h(+)-atpase activity to assess acid tolerance of bacillus sp. for potential probiotic functional attributes. | the present study has been focused widely on comparative account of probiotic qualities of bacillus spp. for safer usage. initially, 170 heat resistant flora were isolated and selected for non-pathogenic cultures devoid of cytk, hbld, and nhe1 virulence genes. subsequently, through biochemical tests along with 16s rrna gene sequencing and fatty acid profiling, the cultures were identified as bacillus megaterium (ar-s4), bacillus subtilis (hr-s1), bacillus licheniformis (csm1-1a and hn-s1), and b ... | 2014 | 25125040 |
| permanent draft genome sequence of bacillus flexus strain t6186-2, a multidrug-resistant bacterium isolated from a deep-subsurface oil reservoir. | previous studies suggest that antibiotic resistance genes have an ancient origin, which is not always linked to the use of antibiotics but can be enhanced by human activities. bacillus flexus strain t6186-2 was isolated from the formation water sample of a deep-subsurface oil reservoir. interestingly, antimicrobial susceptibility testing showed that this strain is susceptible to kanamycin, however, resistant to ampicillin, erythromycin, gentamicin, vancomycin, fosfomycin, fosmidomycin, tetracycl ... | 2014 | 25301038 |
| concomitant production of detergent compatible enzymes by bacillus flexus xju-1. | a soil screened bacillus flexus xju-1 was induced to simultaneously produce alkaline amylase, alkaline lipase and alkaline protease at their optimum levels on a common medium under submerged fermentation. the basal cultivation medium consisted of 0.5% casein, 0.5% starch and 0.5% cottonseed oil as an inducer for protease, amylase, and lipase, respectively. the casein also served as nitrogen source for all 3 enzymes. the starch was also found to act as carbon source additive for both lipase and p ... | 2014 | 25477924 |
| isolation and identification of bacteria to improve the strength of concrete. | the objective of this research work is to isolate and identify calcite precipitating bacteria and to check the suitability of these bacteria for use in concrete to improve its strength. bacteria to be incorporated in concrete should be alkali resistant to endure the high ph of concrete and endospore forming to withstand the mechanical stresses induced in concrete during mixing. they must exhibit high urease activity to precipitate calcium carbonate in the form of calcite. bacterial strains were ... | 2015 | 25946328 |
| arsenite-oxidizing bacteria exhibiting plant growth promoting traits isolated from the rhizosphere of oryza sativa l.: implications for mitigation of arsenic contamination in paddies. | arsenite-oxidizing bacteria exhibiting plant growth promoting (pgp) traits can have the advantages of reducing as-uptake by rice and promoting plant growth in as-stressed soil. a gram-positive bacterium bacillus flexus aso-6 resistant to high levels of as (32 and 280 mm for arsenite and arsenate, respectively) and exhibiting elevated rates of as(iii) oxidation (vmax=1.34 μm min(-1) 10(-7) cell) was isolated from rhizosphere of rice. the presence of aoxb gene and exhibition of as(iii)-oxidase enz ... | 2016 | 26448489 |
| bacillus flexus strain as-12, a new arsenic transformer bacterium isolated from contaminated water resources. | a total of 14 arsenic-resistant bacteria were isolated from an arsenic-contaminated travertine spring water in the central district of qorveh county, kurdistan province, iran. one of strains designated as-12 was selected for further investigation because of its ability to transform arsenic. the strain was identified by cultural, morphological and biochemical tests, and 16s rrna gene sequencing. finally, the growth characteristics of the isolate were investigated in a chemically defined medium wh ... | 2017 | 27912188 |
| study of new feruloyl esterases to understand lipase evolution: the case of bacillus flexus. | recently, the crystal structure of the feruloyl esterase a from aspergillus niger (anfaea) was elucidated. this enzyme displays an α/β hydrolase fold and a catalytic triad similar to that found in fungal lipases (30-37% identity). surprisingly, anfaea showed an overall fold similarity with the rhizomucor miehei and other related fungal lipases. all these data strongly suggest that the ancestral function (lipase) had shifted, with molecular adaptation leading to a novel enzyme (type-a feruloyl es ... | 2012 | 22426711 |
| studies on microbiologically influenced corrosion of ss304 by a novel manganese oxidizer, bacillus flexus. | a manganese oxidizing bacterium was isolated from the surface of steel scraps and biochemical tests and 16s rrna sequencing analysis confirmed the isolate as bacillus flexus. potentiodynamic polarization curves showed ennoblement of open circuit potential, increased passive current, a lowering of breakdown potential, active re-passivation potential and enhanced cathodic current in the presence of b. flexus. adhesion studies with b. flexus on ss304 specimens with different surface treatments demo ... | 2011 | 21749279 |
| biodegradation of 2-nitrotoluene by micrococcus sp. strain smn-1. | a bacterial consortium capable of degrading nitroaromatic compounds was isolated from pesticide-contaminated soil samples by selective enrichment on 2-nitrotoluene as a sole source of carbon and energy. the three different bacterial isolates obtained from bacterial consortium were identified as bacillus sp. (a and c), bacillus flexus (b) and micrococcus sp. (d) on the basis of their morphological and biochemical characteristics and by phylogenetic analysis based on 16s rrna gene sequences. the p ... | 2011 | 20582618 |
| a simple kinetic model for growth and biosynthesis of polyhydroxyalkanoate in bacillus flexus. | polyhydroxyalkanoate (pha), which is produced by several bacteria, is a biodegradable polymer that has many industrial and medical applications. this study deals with development of a simple kinetic model and modification of the logistic equation that can provide an adequate description of pha formation process by bacillus flexus. the parameters studied were kinetics of microbial growth, substrate consumption, and product formation. the microbial growth was described by simplification of monod's ... | 2009 | 19427420 |
| immunostimulatory activity of bacillus spores. | bacillus species, typically bacillus subtilis, are being used as probiotics and mounting evidence indicates that bacillus species are important for development of a robust gut-associated lymphoid system (galt). we used a number of gut isolates of bacillus incorporating three species, b. subtilis, bacillus licheniformis and bacillus flexus to evaluate the nature of interaction between spores and the galt. in mice orally administered with spores, evidence of cell proliferation was determined in th ... | 2008 | 18430003 |
| bacillus koreensis sp. nov., a spore-forming bacterium, isolated from the rhizosphere of willow roots in korea. | an endospore-forming, rod-shaped bacterium was isolated from the rhizosphere of willow roots in korea. the bacterium, designated strain br030t, was a strictly aerobic, motile rod. the cell wall contained type a1gamma peptidoglycan with meso-diaminopimelic acid as the diagnostic diamino acid. the major cellular fatty acids were anteiso-c(15 : 0), iso-c(15 : 0), iso-c(14 : 0) and iso-c(16 : 0). the major cellular phospholipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanola ... | 2006 | 16403867 |
| bacillus herbersteinensis sp. nov. | two bacterial strains, designated d-1,5a(t) and d-1,5b, were isolated from a medieval wall painting in the chapel of castle herberstein, styria (austria). the gram-positive, heterotrophic, aerobic, spore-forming rods showed nearly identical whole-cell protein patterns, identical genomic fingerprints and identical physiological profiles, demonstrating their relationship at the species level. both strains contained meso-diaminopimelic acid in their peptidoglycan, possessed a quinone system compris ... | 2005 | 16166719 |
| bacillus barbaricus sp. nov., isolated from an experimental wall painting. | in a project concerning bacterial colonization of experimental wall paintings, a large number of isolates have been acquired with high similarities in their whole-cell protein patterns obtained after sds-page. of this group, four strains, designated v2-biii-a2(t), v2-bi-a9, v2-bi-04 and v2-bii-a8, were chosen for further characterization. banding patterns obtained after eric-pcr were barely distinguishable among these four strains, indicating their affiliation within a single species. the isolat ... | 2003 | 12807193 |
| purification and properties of beta-cyclomaltodextrin glucanotransferase from bacillus flexus sv 1. | cyclomaltodextrin glucanotransferase is a unique enzyme that degrades starch into cyclic oligosaccharides called cyclodextrins, which have numerous applications in various industries such as pharmaceutical, textile, agricultural, cosmetics etc. due to its wide applications, microorganism producing one type of cyclodextrin is of interest as it simplifies the down streaming process of separating mixture of cyclodextrins. in the present study, β-cgtase was isolated from bacillus flexus sv 1 and bio ... | 2017 | 28857197 |
| antitumor exopolysaccharides derived from novel marine bacillus: isolation, characterization aspect and biological activity | objective: exopolysaccharides gained attention as new source for cancer treatment as recent treatments cause side effects and multidrug resistance. polysaccharides containing sulfur and uronic acids exhibited antioxidant activity, by restoring cell redox regulation, thus inhibiting cell proliferation and cancer formation. following this context, our study was performed to assess the cytotoxic activity of exopolysaccharides produced by novel egyptian marine bacterial strains on hepg2 cells. metho ... | 2017 | 28749119 |
| extractability of polyhydroxyalkanoate synthesized by bacillus flexus cultivated in organic and inorganic nutrient media. | bacillus flexus was isolated from local soil sample and identified by molecular methods. in inorganic nutrient medium (im) containing sucrose as carbon source, yield of biomass and polyhydroxyalkanoate (pha) were 2 g/l and 1 g/l (50% of biomass), respectively. substitution of inorganic nitrogen by peptone, yeast extract or beef extract resulted in biomass yields of 4.1, 3.9 and 1.6 g/l, respectively. corresponding yields of pha in biomass was 30%, 40% and 44%. cells subjected to change in nutrie ... | 2010 | 23100809 |
| synthesis of anisotropic silver nanoparticles using novel strain, bacillus flexus and its biomedical application. | synthesis of metallic nanoparticles has attracted by bacterial based production and alternative to physical and chemical approaches. the present work was focused to nominate a bacterial strain for synthesis of potential silver nanoparticles. the target was achieved by screening of 127 isolates from silver mining wastes. a strain designated s-27 found to be a potential candidate for rapid synthesis of silver nanoparticles among tested microorganisms. it was subjected to molecular characterization ... | 2013 | 23018021 |
| bacterial extracellular polymeric substances and their effect on settlement of zoospore of ulva fasciata. | the extracellular polymeric substances (epss) secreted by bacillus flexus (gu592213) were estimated to have the molecular weight of approximately 1528 and 33,686 kda with the elemental composition of na, p, mg, c, o, cl and s. the (1)h nmr and ft-ir analysis of eps confirmed the presence of different aliphatic and aromatic groups. the eps was amorphous in nature with an average particle size of 13.969 μm (d 0.5) and roughness of 193 nm. the gc-ms analysis has revealed different monosaccharides s ... | 2013 | 23201741 |
| biodegradation of microcystin-rr by bacillus flexus isolated from a saudi freshwater lake. | a bacterium capable of degrading microcystin-rr (mc-rr) was isolated from a saudi eutrophic lake which was previously reported to have microcystin-producing cyanobacteria. based on the analysis of the 16s rrna gene sequence, the isolated strain ssz01, most likely belong to the genus bacillus with a highest sequence similarity (99%) with bacillus flexus strain emga5. it was found that b. flexus strain ssz01, possesses an mlra gene encoding the most important enzyme for mc degradation. this strain ... | 2012 | 23961204 |
| characterization of fluoride-tolerant halophilic bacillus flexus nm25 (hq875778) isolated from fluoride-affected soil in birbhum district, west bengal, india. | a new gram-positive, nonpigmented, rod-shaped fluoride-tolerant bacterial strain, nm25, was isolated from waterlogged muddy field soil collected from the fluoride endemic area of rampurhat ii block (average fluoride in water, 4.7 mg/l, and in soil, 1.5 mg/kg) in birbhum district, west bengal, india. the study was undertaken to characterize the fluoride-tolerant bacterial isolate, to determine its role in bioaccumulation of fluoride, and to analyze the water and soil quality of the bacterial envi ... | 2014 | 24068284 |
| selective inhibition of toxic cyanobacteria by β-carboline-containing bacterium bacillus flexus isolated from saudi freshwaters. | a bacterial strain ssz01 isolated from a eutrophic lake in saudi arabia dominated by cyanobacterial blooms, showed an antialgal activity against cyanobacteria species. based on the analysis of the 16s rdna gene sequence, the isolated strain (ssz01) most likely belonged to the genus bacillus with a 99% similarity to bacillus flexus strain emga5. the thin layer chromatography (tlc) analysis of the ethyl acetate extract of this bacterium revealed that this strain can produce harmine and norharmane ... | 2013 | 24235872 |