| comparison of antigens of pneumonia-associated mycoplasma species by gel diffusion. | comparison of fluorocarbon-extracted antigens of six mycoplasma species by double immunodiffusion and counterimmunodiffusion techniques revealed a close reciprocal relationship among mycoplasma dispar, m. ovipneumoniae, and m. hyopneumoniae. a lesser degree of cross-reaction was also demonstrated between these three species and m. hyorhinis and m. bovoculi. the interrelationships were more clearly demonstrated by double immunodiffusion than by counterimmunodiffusion. | 1978 | 101469 |
| heterogeneity of mycoplasma dispar. | | 1978 | 364965 |
| the effect of an intramammary infusion of endotoxin on experimentally induced mycoplasmal mastitis. | the infusion of 10 microgram of endotoxin lipopolysaccharide from escherichia coli into the mammary gland of four cows 16 h before inoculation with ureaplasmas did not prevent, or even diminish, the subsequent ureaplasmal mastitis. there was no reduction in the severity or duration of the inflammatory cell response in milk or in the clinical appearance of the resulting mastitis. also, the excretion of ureaplasmas was not reduced. a similar experiment with mycoplasma dispar in two cows demonstrat ... | 1979 | 390046 |
| calf pneumonia associated with mycoplasma dispar infection. | | 1979 | 393678 |
| the incidence of mycoplasma dispar, ureaplasma and conventional mycoplasma in the pneumonic calf lung. | this report describes the incidence of mycoplasma dispar, ureaplasma and conventional (large colony) mycoplasma isolated from the pneumonic lungs of groups of young calves and the identification to species level of mycoplasmas in mixed populations with the aid of the indirect fluorescent antibody test. pneumonic lung tissue yielded one or more mycoplasma species from 88% of the 153 calves cultured. the mycoplasmas identified and percent of the calves with lungs positive for each species were: m. ... | 1979 | 398234 |
| isolation of mycoplasma dispar from pneumonic calf lungs in japan. | | 1977 | 560633 |
| experimental mycoplasma mastitis in mice. | thirteen strains of mycoplasma representing six different species, acholeplasma laidlawii, mycoplasma dispar, m. bovirhinis, m. bovigenitalium, m. agalactiae subsp. bovis, and m. mycoides subsp. mycoides, were inoculated into the mammary glands of mice, and the number of mycoplasmas present in the glands three days after inoculation was determined. the predominant response included involution of inoculated glands and a neutrophil infiltration. with the exception of m. dispar, the pathogenicity o ... | 1976 | 776833 |
| induction of pneumonia in gnotobiotic calves following inoculation of mycoplasma dispar and ureaplasmas (t-mycoplasmas). | cloned cultures of mycoplasma dispar and of ureaplasma sp were inoculated endobronchially into gnotobiotic calves. the resulting pneumonia was estimated to involve 2 to 10 per cent of the lung although no clinical signs were observed. the histopathological lesions consisted of infiltration with mononuclear cells, cuffing (peribronchiolar lympho-reticular hyperplasia), catarrhal bronchiolitis and atelectasis. the results indicate that both m dispar and ureaplasma sp are capable of causing sub-cli ... | 1976 | 968191 |
| mycoplasmas and cuffing pneumonia in a group of calves. | the mycoplasmas found in the lungs of 20 calves, housed together for six months, and the related pulmonary pathology are reported. twelve calves had cuffing pneumonia and in this group there was a significantly higher isolation frequency of mycoplasma dispar and ureaplasma spp compared with the non-pneumonic group. mycoplasma bovirhinis and acholeplasma laidlawii were isolated from the lungs of calves in both groups. mycoplasma arginini was not recovered from the lungs of any calf. the significa ... | 1975 | 1198879 |
| experimentally produced calf pneumonia. | experimental pneumonia was produced in calves by the endobronchial inoculation of pneumonic lung homogenates. irradiated homogenates produced minimal pneumonia. ampicillin treatment of the homogenates and the experimental calves reduced the extent of pneumonia. treatment with tylosin tartrate prevented experimental pneumonia. these results suggest that the total pneumonia was due to organisms susceptible to tylosin tartrate and that the residual pneumonia remaining after ampicillin treatment was ... | 1976 | 1265354 |
| the nasal mycoplasmal flora of healthy calves and cows. | the nasal mycoplasmal flora of 270 healthy cows from 27 herds in the netherlands and 35 healthy calves from 7 of these herds was examined. various methods for isolating mycoplasmas were compared. the prevalence of the various species was as follows: ureaplasma diversum in 3 (9%) calves; mycoplasma dispar in 14 (40%) calves; m. bovis in 1 (3%) calf; m. bovirhinis in 23 (66%) calves and 16 (6%) cows; m. bovoculi in 8 (23%) calves and 53 (20%) cows; m. canis in 1 (3%) calf; m. equirhinis in 2 (1%) ... | 1992 | 1462726 |
| a group level analysis of the associations between antibodies to seven putative pathogens and respiratory disease and weight gain in ontario feedlot calves. | the associations, at the group level, between serological titer to pasteurella haemolytica surface antigens (ph), pasteurella haemolytica cytotoxin (ph-cytox), infectious bovine rhinotracheitis virus (ibrv), bovine virus diarrhea virus (bvdv), parainfluenza-3 virus (piv3), respiratory syncytial virus (rsv), mycoplasma dispar (md), m. bovis (mb), and respiratory disease treatment rates, relapse rates, and 28 day weight gains were investigated in 14 groups of calves entering two feedlots during ye ... | 1990 | 2165846 |
| an improved method for the identification of mycoplasma dispar. | an indirect immunoperoxidase test is described for easy and reliable identification of mycoplasma dispar. cultures suspected of being m. dispar were grown on nitrocellulose filter papers and could be identified without having produced centre-forming colonies. these develop only after several passages in vitro and are required for standard identification procedures such as immunofluorescence or growth inhibition. it is to be expected that the method reported here could also be useful for the iden ... | 1987 | 2442880 |
| field trial of a quadrivalent vaccine against calf respiratory disease. | a quadrivalent vaccine containing the killed antigens of respiratory syncytial virus, parainfluenza virus type 3, mycoplasma dispar and m bovis, emulsified with an oil adjuvant, was tested for efficacy against naturally occurring calf respiratory disease. three batches of beef cattle aged 12, seven and three weeks at the time of first vaccination were used. within each batch of approximately 100 animals, half were vaccinated subcutaneously on three occasions, three weeks apart and half served as ... | 1987 | 2825397 |
| the prevalence and level of colonisation by mycoplasma dispar and other mycoplasmas on calf rearing farms. | | 1987 | 3447476 |
| transmissibility of mycoplasma dispar under experimental conditions. | | 1987 | 3447477 |
| transmission of mycoplasma dispar among calves collected and reared for beef production. | | 1987 | 3447478 |
| transmission of mycoplasma dispar among a succession of newborn calves on a dairy farm. | | 1987 | 3454547 |
| growth of mycoplasma bovis in organ cultures of bovine foetal trachea and comparison with mycoplasma dispar. | inoculation of tracheal organ cultures from bovine foetuses with mycoplasma bovis resulted in a loss of cellular structure of the lamina propria, followed 20-22 days later by lifting and detachment of overlying epithelium. the effect was associated with large numbers of m. bovis, identified by immunoperoxidase labelling and electromicroscopy, infiltrating between the epithelial cells and amassing in the lamina propria, especially in the region of the basement membrane of the epithelium. ciliary ... | 1987 | 3551320 |
| an electron-microscopic examination of certain bovine mycoplasmas stained with ruthenium red and the demonstration of a capsule on mycoplasma dispar. | | 1974 | 4139238 |
| serological comparison and haemagglutinating activity of mycoplasma dispar. | a comparison of twelve strains of mycoplasma dispar by the metabolism inhibition and indirect haemagglutination tests has shown them to form a serologically homogeneous group of micro-organisms. the twelve strains vary in their haemagglutinating activity against erythrocytes from different animal species, and certain of the strains can be distinguished by the erythrocytes they agglutinate. haemagglutination may thus provide a method by which certain strains can be typed. the erythrocyte receptor ... | 1974 | 4531453 |
| a subclinical pneumonia of calves associated with mycoplasma dispar. | | 1973 | 4591123 |
| [goat mycoplasma related to mycoplasma dispar species]. | | 1973 | 4601111 |
| inhibition by mycoplasma dispar of ciliary activity in tracheal organ cultures. | the inhibition by mycoplasma dispar of ciliary activity of organ cultures of bovine fetal trachea was found to be dependent on the presence of serum in the maintenance medium. inhibitory activity was unaffected by catalase and no toxic substance was demonstrated in organ culture supernatant fluid. | 1974 | 4604610 |
| enhanced isolation of mycoplasma dispar by substitution of ampicillin for benzylpenicillin in growth media. | | 1973 | 4786127 |
| a new mycoplasma species isolated from pneumonic lungs of calves (mycoplasma dispar sp. nov.). | | 1970 | 5465091 |
| colonisation pattern of the respiratory tract of calves by mycoplasma dispar. | | 1984 | 6534165 |
| longstanding colonisation of the respiratory tract of calves by mycoplasma dispar. | | 1983 | 6660171 |
| immune response of calves following the inoculation of mycoplasma dispar and mycoplasma bovis. | a small but significant reduction in the number of mycoplasma dispar colonising the respiratory tract after intratracheal challenge was observed in gnotobiotic-calves previously inoculated subcutaneously three times with formalin-killed organisms and oil adjuvant. injection of m. dispar by the intramuscular route, with oil adjuvant, and 2 weeks later by the intratracheal route, without adjuvant, failed to induce immunity to subsequent intratracheal challenge. following the subcutaneous injection ... | 1983 | 6845635 |
| mycoplasma dispar as a causative agent in pneumonia of calves. | | 1980 | 7386329 |
| aminopeptidase activity of acholeplasma laidlawii, mycoplasma bovirhinis, mycoplasma dispar and mycoplasma bovis. | aminopeptidase activity was demonstrated in extracts of acholeplasma laidlawii, mycoplasma bovirhinis, m. bovis and m. dispar. the enzyme specificity, which differed amongst the mycoplasma species examined, was characterized using 19 aminoacyl-beta-naphthylamides as substrates. the differences were detected after 4 h incubation with the substrates and potentially could be employed to aid in the characterization of certain mycoplasma species. | 1980 | 7411115 |
| impaired tracheobronchial clearance of bacteria in calves infected with mycoplasma dispar. | infection of tracheal organ cultures with mycoplasma dispar results in degeneration of respiratory epithelial cells with loss of ciliary activity. to assess the effect of these changes on the clearance of bacteria from the respiratory tract, the tracheobronchial clearance of a suspension of serratia marcescens was determined in calves before and after infection with m. dispar. tracheobronchial samples were obtained at various times after deposition of the marker bacteria. clearance of s. marcesc ... | 1994 | 7701860 |
| epidemiological study of enzootic pneumonia in dairy calves in saskatchewan. | a field study involving 325 calves from 17 dairy herds in saskatchewan was conducted to determine the risk of enzootic pneumonia and to assess its association with a number of factors. two different case definitions of pneumonia were used in the analyses: the first was based on producers' treatment risk (case1) and the second was based on semimonthly clinical examinations of calves by the research veterinarian (case2). the risk of pneumonia based on case1 was 39% and on case2 was 29%. the measur ... | 1993 | 8269363 |
| mycoplasma species and related organisms isolated from ruminants in britain between 1990 and 2000. | between 1990 and 2000, more than 1600 mycoplasmas and the related acholeplasmas were identified from ruminant animals by the mycoplasma group at the veterinary laboratories agency--weybridge. mycoplasma bovis was the most commonly identified pathogen, mostly from pneumonic calves but occasionally from cattle with mastitis and arthritis. mycoplasma canis was first isolated in britain in 1995 from pneumonic calves and the number of isolates increased to 18 per cent of the total mycoplasmas isolate ... | 2004 | 15508840 |
| rapid detection of mycoplasma dispar and m. bovirhinis using allele specific polymerase chain reaction protocols. | we describe an allele specific pcr based approach for the rapid detection of two bovine mycoplasma species associated with respiratory disease. specific and universal oligonucleotides were used in combination to detect the presence of single nucleotide polymorphisms within the 16s ribosomal dna sequence. presence of mycoplasma 16s rdna is indicated by the production of a single control fragment, whilst positive samples generate an alternative smaller specific product over the same region. this t ... | 2004 | 15556716 |
| biochemical characterization of mycoplasma bovirhinis, mycoplasma dispar and recent bovine isolates of mycoplasma canis. | the pattern and kinetics of substrate utilization by the type strains of mycoplasma canis, m. bovirhinis and m. dispar and ten recent m. canis isolates from cattle were determined. metabolism of a range of sugars and organic acids by m. dispar was detectable by measurement of oxygen uptake. organic acids were not utilized by m. bovirhinis or m. canis, and there was no oxygen uptake during metabolism of glucose or other sugars, as monitored by a ph-change method. the m. canis strains varied in th ... | 2001 | 11214667 |
| susceptibilities of mycoplasma bovis, mycoplasma dispar, and ureaplasma diversum strains to antimicrobial agents in vitro. | the purpose of this study was to determine the susceptibility of various strains of mycoplasma bovis, mycoplasma dispar, and ureaplasma diversum, which are prevalent causes of pneumonia in calves, to 16 antimicrobial agents in vitro. the mics of the antimicrobial agents were determined by a serial broth dilution method for 16 field strains and the type strain of m. bovis, for 19 field strains and the type strain of m. dispar, and for 17 field strains of u. diversum. final mics for m. bovis and m ... | 1993 | 8452363 |
| use of a polymerase chain reaction for detection of mycoplasma dispar in the nasal mucus of calves. | a polymerase chain reaction (pcr) assay was developed for the detection of mycoplasma dispar in nasal mucus samples collected from calves. the target dna sequence was the 16s rrna gene, and the fragment was selected within a region of high polymorphism. the specificity and detection limit of the method were determined. this method was then used for the detection of m. dispar in nasal swabs collected from 301 calves, including 155 clinical samples from animals showing signs of respiratory disease ... | 2007 | 17459842 |
| acute fatal pneumonia in calves due to respiratory syncytial virus. | an acute pneumonia developed in 28 calves which had been housed together from one to two weeks of age. the clinical signs included pyrexia, tachypnoea, respiratory distress and coughing. some of the calves died. the pneumonia was characterised by an alveolitis with multinucleated syncytia, alveolar epithelial hyperplasia and bronchiolitis. interstitial emphysema was also present. fifteen of 19 calves examined serologically had rising neutralising antibody titres to respiratory syncytial virus; i ... | 1981 | 7257127 |
| inhibition of growth of mycoplasma dispar by dl-alpha-difluoromethyllsine, a selective irreversible inhibitor of lysine decarboxylase, and reversal by cadaverine (1,5-diaminopentane). | dl-alpha-difluoromethyllysine was shown to be a potent inhibitor of lysine decarboxylase from mycoplasma dispar. the inhibition appeared to be specific since neither difluoromethylornithine nor difluoromethylarginine, known to inhibit other decarboxylases, inhibited the reaction catalyzed by lysine decarboxylase in extracts of m. dispar. inhibition was irreversible since extensive dialysis could not overcome the inhibitory effect exerted by difluoromethyllysine. difluoromethyllysine (1 mm) also ... | 1984 | 6439203 |
| molecular epidemiological analysis of mycoplasma bovis isolates from the pennsylvania animal diagnostic laboratory showing genetic diversity. | we have examined the genetic variability of mycoplasma bovis strains submitted to the pennsylvania animal diagnostics laboratory, university park (pa-adl), between december 2007 and december 2008. of 4,868 total samples submitted for mycoplasma testing, 302 were determined to be culture positive. mycoplasma bovis (63.6%), mycoplasma californicum (7.3%), mycoplasma bovirhinis (2.7%), mycoplasma bovigenitalium (0.7%), mycoplasma alkalescens (4.9%), mycoplasma putrefaciens (0.3%), and mycoplasma di ... | 2011 | 21426978 |
| effect of mycoplasma dispar, mycoplasma bovirhinis, acholeplasma laidlawii and t-mycoplasmas on explant cultures of bovine trachea. | | 1974 | 4604399 |
| the association between serological evidence of mycoplasma infection and respiratory disease in feedlot calves. | calves from five ontario feedlots were bled on arrival and approximately 28 days later. calves treated during this interval for undifferentiated respiratory disease were classified as cases and untreated calves were classified as controls. serum was titrated blindly for antibodies to mycoplasma bovis and mycoplasma dispar. indirect hemagglutination titers of 1:20 or more were assumed to reflect recent or current exposure, whereas 1:10 or less were not. the titers to m. bovis increased in all fee ... | 1986 | 3756671 |
| identification of mycoplasmatales in pneumonic calf lungs. | lungs from 153 calves with clinical signs of pneumonia were examined post-mortem (pm) for the presence of mycoplasmas and ureaplasmas during a 38-month period. sixty-two percent of the cases were submitted during the months when wide fluctuations in climatic conditions occur. using indirect fluorescent antibody tests (ifat) and culture, mycoplasmas and/or ureaplasmas were detected in 63% of the lungs examined. mycoplasma dispar was detected in 39%, m. bovis in 36%, ureaplasma spp. in 22% and m. ... | 1986 | 3518226 |
| growth and interaction of mycoplasma bovirhinis and mycoplasma dispar in vitro. | two mycoplasmas were grown in pure and mixed cultures in glucose calf-serum broth with initial ph 7.8. sensitivity to ph was also tested. the main data for mycoplasma bovirhinis and mycoplasma dispar, respectively, in pure cultures were as follows: lag phase, days: less than 1, 1-2; log phase: 1-2, 1-4; relatively stationary phase: 0-1, 2-4; decline phase (to the extent roughly logarithmic): 2-6, 5-10; maximal titers: 5 x 10(7) to 10(9), 5 x 10(6) to 10(8) color changing units per 0.2 ml; highes ... | 1987 | 3322657 |
| comparative pathogenicity of mycoplasma bovis and mycoplasma dispar for the respiratory tract of calves. | mycoplasma bovis and m. dispar are both important in the etiology of calf respiratory disease, but marked differences are evident in the pathogenesis of disease caused by the two species. both attach to bovine cells but vary in their invasiveness. m. bovis is capable of invading the lung parenchyma from the airways, while m. dispar predominantly infects the ciliated epithelial cells lining the respiratory tract. m. dispar causes ciliostasis in fetal-bovine tracheal organ cultures, whereas m. bov ... | 1987 | 3312099 |
| cadaverine covalently linked to a peptidoglycan is an essential constituent of the peptidoglycan necessary for the normal growth in selenomonas ruminantium. | cadaverine links covalently to the d-glutamic acid residue of the peptidoglycan in selenomonas ruminantium, a strictly anaerobic gram-negative bacterium (kamio, y., itoh, y., and terawaki, y. (1981) j. bacteriol. 146, 49-53). this report clarifies a physiological function of cadaverine in this organism by using dl-alpha-difluoromethyllysine, which had previously been shown to be a selective irreversible inhibitor of lysine decarboxylase of mycoplasma dispar (pösö, h., macann, p.p., tanskanen, r. ... | 1986 | 3084485 |
| capsulelike surface material of mycoplasma dispar induced by in vitro growth in culture with bovine cells is antigenically related to similar structures expressed in vivo. | electron microscopy has been used to show that mycoplasma dispar produces an external capsulelike material in vivo that has an affinity for both ruthenium red and polycationic ferritin. this extracellular material is lost upon passage in culture medium but can be regained with a single passage on bovine lung fibroblast (blf) cells. to confirm that the extracellular material associated with cell-grown mycoplasmas was the same as that observed in infected calves, rabbit antibodies were produced to ... | 1991 | 1715319 |
| interaction of mycoplasma dispar with bovine alveolar macrophages. | the capacity to avoid phagocytosis and the activation of bovine alveolar macrophages (bam) by encapsulated mycoplasma dispar or purified m. dispar capsule was investigated. encapsulated and unencapsulated m. dispar were cocultured with bam in the presence or absence of antisera prepared against unencapsulated m. dispar or purified capsule antiserum. unopsonized mycoplasmas resisted phagocytosis, while only anti-capsule antibodies enhanced the phagocytosis of encapsulated mycoplasmas. bam were cu ... | 1992 | 1612758 |
| prevalence of mycoplasmas in the respiratory tracts of pneumonic calves. | the prevalence of mycoplasmas in the respiratory tracts of 148 pneumonic calves originating from 25 herds in the netherlands is reported. four types of culture media were used to isolate mycoplasmas: solid modified edward medium, 2 types of friis media, and a7b differential agar medium. mycoplasmas were isolated both from nasal swab specimens and lung lavage fluids collected from live calves and from nasal mucosa and lung tissue specimens collected post mortem. all of the mycoplasma strains isol ... | 1992 | 1462720 |
| localized immunity in experimental bovine mastitis caused by mycoplasma dispar. | development of immunity to reinfection with mycoplasma dispar occurred in the bovine mammary gland and appeared to be confined to the quarter or quarters previously infected with this mycoplasma; it was not generalized throughout the gland. immunity followed the inoculation of virulent strains of m. dispar but not of an avirulent strain. the immunity engendered protected against both homologous and heterologous strains of m. dispar. challenge of immune quarters with virulent m. dispar resulted i ... | 1975 | 1193732 |
| isolation of mycoplasma dispar from cattle in the united states. | | 1975 | 1154634 |
| interaction of mycoplasma dispar and mycoplasma agalactiae subsp. bovis with bovine alveolar macrophages and bovine lacteal polymorphonuclear leukocytes. | mycoplasma dispar and mycoplasma agalactiae subsp. bovis survived or grew in cultures of bovine lacteal polymorphonuclear leukocytes or bovine alveolar macrophages. in the presence of specific bovine antibody, macrophages and polymorphonuclear leukocytes appeared to kill both species of mycoplasma. specific rabbit antisera also promoted the killing of these mycoplasmas by bovine macrophages but had no demonstrable activity for bovine polymorphonuclear leukocytes. it is suggested that phagocytosi ... | 1976 | 985802 |
| pathogenicity of certain mycoplasma species in the bovine mammary gland. | strains of three of the species of mycoplasmas isolated from pneumonic calf lungs, mycoplasma dispar, mycoplasma bovirhinis and acholeplasma laidlawii, were inoculated into the bovine mammary gland. pathogenicity of the species was monitored by counting the inflammatory cells and mycoplasmas in milk. six of the seven m dispar strains produced clinical mastitis, but the seventh strain, vic12, was apparently avirulent. the four strains of m bovirhinis caused subclinical mastitis. none of the four ... | 1976 | 935661 |
| viral and bacterial pathogens in bovine respiratory disease in finland. | pathogens causing bovine respiratory tract disease in finland were investigated. eighteen cattle herds with bovine respiratory disease were included. five diseased calves from each farm were chosen for closer examination and tracheobronchial lavage. blood samples were taken from the calves at the time of the investigation and from 86 calves 3-4 weeks later. in addition, 6-10 blood samples from animals of different ages were collected from each herd, resulting in 169 samples. serum samples were t ... | 2004 | 15663079 |
| isolation of mycoplasma species from the lower respiratory tract of healthy cattle and cattle with respiratory disease in belgium. | between 1997 and 2000, a total of 150 healthy cattle and 238 animals with respiratory disease were examined for six mycoplasma species. attempts were made to detect mycoplasma canis, mycoplasma dispar and ureaplasma diversum in calves with recurrent disease, and all three of these species were identified in calves with recurrent disease and in healthy lungs. in healthy calves, 84 per cent of bronchoalveolar lavage fluids were mycoplasma free; when cultures were positive, mycoplasma bovirhinis wa ... | 2002 | 12418530 |
| comparison of sampling procedures for isolating pulmonary mycoplasmas in cattle. | three sampling procedures were compared to determine the optimal technique for isolating mycoplasmas in cattle with respiratory diseases. the prevalence of mycoplasmas isolated from these animals is also reported. in the first group, bronchoalveolar lavage (bal) and nasal swab cultures were compared with the corresponding lung cultures from cattle necropsied for fatal respiratory diseases (n = 20). in a second group, nasal swabs were compared with corresponding bal cultures in living animals wit ... | 2002 | 12212722 |
| the associations of viral and mycoplasmal antibody titers with respiratory disease and weight gain in feedlot calves. | blood samples from 32 groups of calves (n = 700) were taken on arrival and after 28-35 days at the feedlot. eleven groups were housed in feedlots in ontario, and 21 groups in feedlots in alberta. serum antibody titers to bovine viral diarrhea virus (bvdv), bovine respiratory syncytial virus (brsv), parainfluenza virus type 3 (piv-3), infectious bovine rhinotracheitis virus (ibrv), mycoplasma dispar and m. bovis, plus data on bovine corona virus (bcv) from a previous study were investigated for t ... | 1999 | 12001336 |
| isolation of mycoplasma dispar from mastitis in dry cows. | | 1983 | 16030956 |
| aerosol challenge of calves with haemophilus somnus and mycoplasma dispar. | the aim of the study was to examine the ability of haemophilus somnus and mycoplasma dispar to induce pneumonia in healthy calves under conditions closely resembling the supposed natural way of infection, viz. by inhalation of aerosol droplets containing the microorganisms. the infections were investigated by recording clinical data, cytokine expression of peripheral blood cells and pathology. twelve calves were included in the study: three animals were exposed to h. somnus only, and two to m. d ... | 2000 | 10727833 |
| amplified-fragment length polymorphism fingerprinting of mycoplasma species. | amplified-fragment length polymorphism (aflp) is a whole-genome fingerprinting method based on selective amplification of restriction fragments. the potential of the method for the characterization of mycoplasmas was investigated in a total of 50 strains of human and animal origin, including mycoplasma genitalium (n = 11), mycoplasma pneumoniae (n = 5), mycoplasma hominis (n = 5), mycoplasma hyopneumoniae (n = 9), myco plasma flocculare (n = 5), mycoplasma hyosynoviae (n = 10), and mycoplasma di ... | 1999 | 10488196 |
| epidemiologic and pathologic characteristics of respiratory tract disease in dairy heifers during the first three months of life. | to describe the incidence of respiratory tract disease in dairy calves and to compare antibody titers and microbial isolates from transtracheal wash samples between calves with and without respiratory tract disease (cases and controls, respectively). | 1996 | 8707680 |
| immune responses to the capsular polysaccharide of mycoplasma dispar in calves and mice. | humoral and cell-mediated immune responses to the capsular polysaccharide (cps) of m. dispar and polygalacturonic acid (pgaiu--a structurally similar polysaccharide) were investigated in calves experimentally infected with mycoplasma dispar and in mice immunized with cps or pgaiu. sera, tracheobronchial lavage and nasal fluids, collected before and after infection in calves, were checked for the presence of anti-cps and anti-pgaiu antibodies. the sera from mice injected with cps or pgaiu were ch ... | 1995 | 8549115 |
| respiratory disease in calves: microbiological investigations on trans-tracheally aspirated bronchoalveolar fluid and acute phase protein response. | trans-tracheal aspirations from 56 apparently healthy calves and 34 calves with clinical signs of pneumonia were collected in six different herds during september and november 2002. the 90 samples were cultivated and investigated by pcr tests targeting the species histophilus somni, mannheimia haemolytica, pasteurella multocida, mycoplasma bovis, mycoplasma dispar, and mycoplasma bovirhinis. a pcr test amplifying the lktc-artj intergenic region was evaluated and shown to be specific for the two ... | 2009 | 19186010 |
| acute phase protein changes in calves during an outbreak of respiratory disease caused by bovine respiratory syncytial virus. | bovine acute phase proteins (apps), lipopolysaccharide binding protein (lbp), serum amyloid a (saa), haptoglobin (hp) and alpha(1)-acid glycoprotein (agp) were evaluated as inflammatory markers during an outbreak of bovine respiratory disease (brd) caused by bovine respiratory syncytial virus (brsv). calves (n = 10) presented mild to moderate signs of respiratory disease. secondary bacterial infections, pasteurella multocida and mycoplasma dispar as major species, were detected in tracheobronchi ... | 2011 | 19897247 |
| laboratory diagnosis of mycoplasma infection in young cattle. | the aim of this study was to detect mycoplasma species in the respiratory tract of 110, 310 and 510 day-old groups of cattle by serological, bacteriological and histopathological investigations. antibodies against m. bovis were found in 75% of the 110 day-old, in 50%--of the 310 day-old and in 55%--of the 510 day-old groups of cattle. bacteriological examination of the samples from nasal cavities revealed that mycoplasma carriers were found in 60% of the 110 day-old group of cattle, 40% of the 3 ... | 2011 | 21528717 |
| determination of bacterial aetiologic factor on tracheobronchial lavage in relation to clinical signs of bovine respiratory disease. | this study aimed to determine the occurrence of mannheimiahaemolytica, pasteurella multocida and mycoplasma spp., in relation to clinical signs of respiratory disease. tracheobronchial lavage samples were collected from 96 (healthy and unhealthy) cattle in the state of são paulo, brazil. mycoplasma spp. (12.5 %) and pasteurellamultocida (15.50 %) were the most prevalent species. bacillus sp., staphylococcus sp., escherichia coli, klebsiella oxytoca, pseudomonas aeruginosa and klebsiella pneumoni ... | 2016 | 27582268 |