isolation and characterization of new chlamydosporol related metabolites of fusarium chlamydosporum and fusarium tricinctum.fusarium chlamydosporum strain t-826 isolated from corn in the usa produced chlamydosporol and two analogs which have been identified by various spectroscopic techniques as: 7,8-dihydro-5-hydroxy-4-methoxy-trans-7,8-dimethyl-2h,5h-pyrano(4, 3-b)pyran-2-one (or isochlamydosporol) and 4-methoxy-5-hydroxymethyl-6-(3-butan-2-ol)-2h-pyran-2-one (or chlamydospordiol). chlamydosporol (compound a + b) chlamydospordiol (compound c) and isochlamydosporol (compound d) were produced together (up to 6000 mic ...19947984219
in vitro sensitivity of medically significant fusarium species to various antimycotics.sixteen isolates belonging to fusarium chlamydosporum (n = 4), fusarium equiseti (n = 1), fusarium moniliforme (n = 2), fusarium oxysporum (n = 3), fusarium proliferatum (n = 1), and fusarium solani (n = 5) were tested against amphotericin b, 5-fluorocytosine, fluconazole, itraconazole, ketoconazole, jai-amphotericin b (water-soluble compound), hamycin and amphotericin b combined with 5-fluorocytosine, using antibiotic medium m3, high-resolution broth (ph 7.1), sabouraud's dextrose, and yeast-ni ...19948082411
isoelectric focusing isozyme profiles and taxonomic distances among fusarium species of the sections arthrosporiella and sporotrichiella.isozymes from 18 isolates representing seven species of the fusarium sections arthrosporiella and sporotrichiella were compared by isoelectric focusing in polyacrylamide gels. of the six enzyme systems tested esterase and malate dehydrogenase showed the largest variation. a numerical analysis of the pi values determined for acid phosphatase, esterase, glucose-6-phosphate dehydrogenase, malate dehydrogenase, phosphoglucose isomerase and phosphoglucomutase resulted in a dendrogam demonstrating the ...19938304009
invasive infection with fusarium chlamydosporum in a patient with aplastic anemia.we report the first case of invasive disease caused by fusarium chlamydosporum. the patient had aplastic anemia with prolonged neutropenia and was treated with immunosuppressive therapy. while she was receiving empirical amphotericin b, a dark crusted lesion developed on her nasal turbinate. histologic analysis revealed invasive hyaline hyphae and some darkly pigmented structures that resembled conidia of dematiaceous molds. only after the mold was grown in culture were characteristic colonial m ...19989620419
fusarium species from nepalese rice and production of mycotoxins and gibberellic acid by selected species.infection of cereal grains with fusarium species can cause contamination with mycotoxins that affect human and animal health. to determine the potential for mycotoxin contamination, we isolated fusarium species from samples of rice seeds that were collected in 1997 on farms in the foothills of the nepal himalaya. the predominant fusarium species in surface-disinfested seeds with husks were species of the gibberella fujikuroi complex, including g. fujikuroi mating population a (anamorph, fusarium ...200010698766
factors determining accumulation of mycotoxin producers in cereal grain during harvesting.during the meteorologically contrasting period of 2003-2005, the contamination of winter wheat, malt barley and fodder barley grain with micromycetes during grain harvesting and preparation for storage was investigated. micromycetes of over 70 species ascribed to 16 genera were isolated and identified, the density of their populations in grain was determined. micromycetes with a population density of >50% were attributed to dominant species. short biological characteristic, ecological peculiarit ...200717655196
changes in fungi and mycotoxins in pearl millet under controlled storage conditions.pearl millet is increasingly being grown as a premium-value grain for the recreational wildlife and poultry industries in the southern us. we conducted three experiments to assess grain mold development in storage conditions typically encountered in the region of production. variables included production year, temperature, relative humidity, atmosphere, and grain moisture content. in the first experiment, grain was stored for 9 weeks at 20 or 25 degrees c and maintained at 86% or 91% relative hu ...200717701446
interaction of rotylenchulus reniformis with seedling disease pathogens of cotton.the impact of 10 fusarium species in concomitant association with rotylenchulus reniformis on cotton seedling disease was examined under greenhouse conditions. in experiment 1, fungal treatments consisted of fusarium chlamydosporum, f. equiseti, f. lateritium, f. moniliforme, f. oxysporum, f. oxysporum f.sp. vasinfectum, f. proliferatum, f. semitectum, f. solani, and f. sporotrichioides; rhizoctonia solani; and thielaviopsis basicola. the experimental design was a 2 x 14 factorial consisting of ...200419262802
successful use of posaconazole to treat invasive cutaneous fungal infection in a liver transplant patient on sirolimus.fungi are an important and common cause of cutaneous infections affecting solid organ transplant recipients. these infections can represent a primary site of infection with the potential for dissemination, or a manifestation of metastatic infection. the high morbidity and mortality associated with these infections necessitates urgent therapy with antifungal drugs; however, the interaction between these drugs and immunosuppressive therapies can be a major limitation because of drug toxicity. a ca ...201223730320
detoxification of toxic phorbol esters from malaysian jatropha curcas linn. kernel by trichoderma spp. and endophytic fungi.the presence of phorbol esters (pes) with toxic properties limits the use of jatropha curcas kernel in the animal feed industry. therefore, suitable methods to detoxify pes have to be developed to render the material safe as a feed ingredient. in the present study, the biological treatment of the extracted pes-rich fraction with non-pathogenic fungi (trichoderma harzianum jq350879.1, t. harzianum jq517493.1, paecilomyces sinensis jq350881.1, cladosporium cladosporioides jq517491.1, fusarium chla ...201424504029
less-frequent fusarium species of clinical interest: correlation between morphological and molecular identification and antifungal susceptibility.forty-eight fusarium isolates morphologically identified as belonging to seven species of clinical interest (i.e., fusarium chlamydosporum, fusarium dimerum, fusarium incarnatum, fusarium napiforme, fusarium nygamai, fusarium proliferatum, and fusarium sacchari) were characterized molecularly by the analysis of the sequences of the tub region of the beta-tubulin gene. f. chlamydosporum and f. dimerum were the most genetically heterogeneous species. a high degree of correlation between the morpho ...200919321723
concurrent fusarium chlamydosporum and microsphaeropsis arundinis infections in a cat.a 7-year-old cat was presented initially with multiple draining sinuses on the metatarsal region of its right hindlimb. another lesion had appeared at the same time on the fifth proximal interphalangeal joint of the left forelimb. histopathological examination of a biopsy from the right hindlimb lesion revealed chronic pyogranulomatous inflammation associated with yeast-like bodies and septate mycelia; a fungus was cultured on conventional media but not identified further. culture of a swab coll ...200415265482
purification, characterization, and antifungal activity of chitinase from fusarium chlamydosporum, a mycoparasite to groundnut rust, puccinia arachidis.chitinase (ec was isolated from the culture filtrate of fusarium chlamydosporum and purified by ion-exchange chromatography and gel filtration. the molecular mass of purified chitinase was 40 kda as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. chitinase was optimally active at a ph of 5 and stable from ph 4 to 6 and up to 40 degrees c. among the metals and inhibitors tested, mercuric chloride completely inhibited the enzyme activity. the activity of chitinase ...19989783424
in-vitro antifungal susceptibility of clinical and environmental fusarium spp. strains.the mics of amphotericin b, miconazole, ketoconazole, flucytosine, itraconazole and fluconazole for 19 isolates of fusarium oxysporum, 16 fusarium solani, seven fusarium verticilliodes, four fusarium proliferatum, four fusarium dimerum, three fusarium equiseti, and one each of the following species: fusarium graminearum, fusarium chlamydosporum, fusarium semitectum, fusarium avenaceum and fusarium subglutinans were determined by a broth microdilution method. thirty-eight of these isolates were o ...19979069536
an exoantigen test for the rapid identification of medically significant fusarium species.the accurate identification of fusarium species can take 2-3 weeks. preliminary exoantigen studies indicate that a mature culture suspected of being a fusarium species may be immunologically identified 48 h after receipt. exoantigen extracts of 10-day-old slant cultures of fusarium chlamydosporum, fusarium moniliforme (= fusarium verticilloides), fusarium oxysporum, fusarium proliferatum and fusarium solani and partially purified reference homologous and heterologous shake culture extracts (6-we ...19958544080
catheter-associated fungemia caused by fusarium chlamydosporum in a patient with lymphocytic lymphoma.a case of catheter-associated fungemia caused by fusarium chlamydosporum is described in a patient with lymphocytic lymphoma. the fungus, which has been isolated from soil but not reported to cause human infection, characteristically produces microconidiophores that are polyphialides bearing microconidia that are spindle-shaped but never globose. results of in vitro antimicrobial susceptibility tests depended on the test conditions used.19853838757
chlamydosporol, a new metabolite from fusarium chlamydosporum.extracts of rice on which an isolate of fusarium chlamydosporum had been cultured were toxic to brine shrimps. the toxic fraction was purified by flash chromatography to give two compounds which were identified by uv, ir, nmr and mass spectroscopy at the 6 alpha and 6 beta isomers of 5-hydroxy-4-methoxy-6,8a-dimethyl-6,7-dihydro-2h, 8ah-pyrano[2,3-b]pyran-2-one. these lactones for which the name chlamydosporol is proposed have not been reported previously. when tested in brine shrimp and hela ce ...19902388683
toxicity of some fusarium section sporotrichiella strains in relation to mycotoxin production.the relationship between the toxicities of crude extracts and purified toxins of fusarium spp. belonging to the section sporotrichiella has been assessed. toxicity was determined on the basis of death of artemia salina larvae and of viability and blastogenic response of bovine and human lymphocytes. trichothecene-producing strains of fusarium sporotrichioides and fusarium poae were toxic to a. salina and to lymphocyte blastogenesis. a strain of fusarium tricinctum, producing visoltricin and chla ...19921610205
synergy between glomus fasciculatum and a beneficial pseudomonas in reducing root diseases and improving yield and forskolin content in coleus forskohlii briq. under organic field conditions.root rot and wilt, caused by a complex involving fusarium chlamydosporum (frag. and cif.) and ralstonia solanacearum (smith), are serious diseases affecting the cultivation of coleus forskohlii, a crop with economic potential as a source of the medicinal compound forskolin. the present 2-year field experiments were conducted with two bioinoculants (a native pseudomonas monteilii strain and the exotic arbuscular mycorrhizal (am) fungus glomus fasciculatum) alone and in combination under organic f ...201322648372
synergysm of voriconazole or itraconazole with other antifungal agents against species of fusarium.infections caused by fusarium are difficult to treat because these fungi show in vitro and in vivo resistance to practically all the antifungal agents available, which explains the high mortality rates. an attempt to overcome fungal resistance is the combination of antifungal agents, especially those with different mechanisms of action.201623402831
perinephric abscess caused by fusarium chlamydosporum in an immunocompetent child: case report and identification of the morphologically atypical fungal strain.fusarium infections are important problem worldwide, cause a broad spectrum of infections in human including superficial infections as well as locally invasive and disseminated infections. we report a rare case of perinephric abscess caused by fusarium chlamydosporum in a child who had a recent episode of pyelonephritis. this case illustrates the ever increasing spectrum of rare but offending pathogenic fungi in an immunocompetent host. fungal infections should always be suspected in patients ha ...201324152521
morphological and molecular characterization of fusarium spp pathogenic to pecan tree in brazil.the occurrence of fusarium spp associated with pecan tree (carya illinoinensis) diseases in brazil has been observed in recent laboratory analyses in rio grande do sul state. thus, in this study, we i) obtained fusarium isolates from plants with disease symptoms; ii) tested the pathogenicity of these fusarium isolates to pecan; iii) characterized and grouped fusarium isolates that were pathogenic to the pecan tree based on morphological characteristics; iv) identified fusarium spp to the species ...201425501150
use of mass spectrometry to identify clinical fusarium isolates.fusarium spp. have recently emerged as significant human pathogens. identification of these species is important, both for epidemiological purposes and for patient management, but conventional identification based on morphological traits is hindered by major phenotypic polymorphism. in this study, 62 strains, or isolates, belonging to nine fusarium species were subjected to both molecular identification tef1 gene sequencing and matrix-assisted laser desorption ionization-time-of-flight (maldi-to ...200919456834
inhibitory effects of na-hypochlorite and heating on the mycobiota associated with fruits or juice of passion (passiflora edulis sims) in uganda.a total of 34 species belonging to 21 genera of fungi were recorded on passion fruits of both pure and hybrid origin in uganda, however, the pure type exhibited wider spectrum (28 species and 16 genera) than the hybrid type (21 & 15). also, yeasts (unidentified and rhodotorula mucilaginosa) were also encountered in high numbers. moreover, the mean count of all mycobiota obtained from the pure type was higher than that of hybrid, despite the bigger size of the later. members of yeasts and cladosp ...200624039477
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