| molecular identification of microorganisms from endodontic infections. | a relatively wide range of bacteria have been isolated from root canals using standard culture techniques. however, only 50% of the bacteria in the oral cavity are cultivable (s. s. socransky et al., arch. oral biol. 8:278-280, 1963); hence, bacterial diversity in endodontic infections is underestimated. this study used a pcr-based 16s rrna gene assay, followed by cloning and sequencing of 16s rrna amplicons from a small subset of samples to assess the diversity of bacteria present in infected r ... | 2001 | 11526164 |
| characterisation of eubacterium-like strains isolated from oral infections. | the genus eubacterium currently includes a heterogeneous group of gram-positive, non-spore-forming anaerobic bacilli, many of which are slow growing, fastidious and generally unreactive in biochemical tests. as a consequence, cultivation and identification of isolates are difficult and the taxonomy of the group remains indifferent. in this study, 105 isolates from odontogenic infections, infections associated with dental implants or saliva from healthy subjects and provisionally assigned to the ... | 2001 | 11699590 |
| gram-positive anaerobic bacilli in human periodontal disease. | the uncertain taxonomy of oral anaerobic gram-positive bacilli and their generally slow growing nature has limited the understanding of their role in periodontal disease. the current objective was to design and use species-specific oligonucleotide probes to investigate the relationship of selected gram-positive anaerobic bacilli to periodontal disease. | 2004 | 15206913 |
| dna-dna relatedness and phylogenetic positions of slackia exigua, slackia heliotrinireducens, eggerthella lenta, and other related bacteria. | recently, two asaccharolytic eubacterium species, eubacterium exiguum and eubacterium lentum, and peptostreptococcus heliotrinreducens have been reclassified as slackia exigua, eggerthella lenta and slackia heliotrinireducens in the novel genera on the basis of 16s rdna sequence analysis. but dna-dna relatedness among these species and other related bacteria have not been reported yet. dna-dna relatedness is the standard arbiter and the recommended method for the designation and evaluation of ne ... | 2004 | 15327650 |
| detection frequency of periodontitis-associated bacteria by polymerase chain reaction in subgingival and supragingival plaque of periodontitis and healthy subjects. | the aim of this study was to compare the detection frequencies of 25 bacterial species in subgingival and supragingival plaque of 18 untreated periodontitis subjects and 12 periodontally healthy subjects. genomic dna was extracted from subgingival and supragingival plaque samples, and bacterial detection was performed by polymerase chain reaction of the 16s rrna genes. fourteen bacteria showed no relationship with periodontitis, and 11 of these 14 species were frequently detected (> or =50%) in ... | 2004 | 15491463 |
| identification of bacteria in endodontic infections by sequence analysis of 16s rdna clone libraries. | a significant proportion of oral bacteria are unable to undergo cultivation by existing techniques. in this regard, the microbiota from root canals still requires complementary characterization. the present study aimed at the identification of bacteria by sequence analysis of 16s rdna clone libraries from seven endodontically infected teeth. samples were collected from the root canals, subjected to the pcr with universal 16s rdna primers, cloned and partially sequenced. clones were clustered int ... | 2006 | 16388037 |
| molecular analysis of bacteria in asymptomatic and symptomatic endodontic infections. | the purpose of the present study was to use terminal restriction fragment length polymorphism analysis and the 16s rrna gene clone library to investigate the diversity of the microbiota associated with asymptomatic and symptomatic endodontic infections and to compare the bacterial community structure in these two clinical conditions. samples were taken from asymptomatic endodontic infections associated with chronic periradicular lesions and from symptomatic infections clinically diagnosed as acu ... | 2006 | 16476021 |
| the microbiology of the acute dental abscess. | the acute dental abscess is frequently underestimated in terms of its morbidity and mortality. the risk of potential serious consequences arising from the spread of a dental abscess is still relevant today with many hospital admissions for dental sepsis. the acute dental abscess is usually polymicrobial comprising facultative anaerobes, such as viridans group streptococci and the streptococcus anginosus group, with predominantly strict anaerobes, such as anaerobic cocci, prevotella and fusobacte ... | 2009 | 19141730 |
| comparisons of subgingival microbial profiles of refractory periodontitis, severe periodontitis, and periodontal health using the human oral microbe identification microarray. | this study compared the subgingival microbiota of subjects with refractory periodontitis (rp) to those in subjects with treatable periodontitis (grs = good responders) or periodontal health (ph) using the human oral microbe identification microarray (homim). | 2009 | 19722792 |
| diversity and abundance of the bacterial 16s rrna gene sequences in forestomach of alpacas (lama pacos) and sheep (ovis aries). | two bacterial 16s rrna gene clone libraries were constructed from the forestomach of alpacas and sheep fed alfalfa. after the amplification using the universal 16s rrna gene primers, equal quantities of pcr products from the same species were mixed and used to construct the two libraries. sequence analysis showed that the 60 clones from alpacas were divided into 27 phylotypes with 25% clones affiliated with eubacterium sp. f1. the 60 clones from sheep were divided into 21 phylotypes with 7 phylo ... | 2010 | 20558310 |
| description of mogibacterium pumilum gen. nov., sp. nov. and mogibacterium vescum gen. nov., sp. nov., and reclassification of eubacterium timidum (holdeman et al. 1980) as mogibacterium timidum gen. nov., comb. nov. | a new genus, mogibacterium, is proposed for anaerobic, non-spore-forming, gram-positive, rod-shaped bacteria which have been isolated from the periodontal pockets of adult human patients with periodontal disease and infected root canals. the novel isolates, strains d2-18t, ba11a-f and d5-2t, were inert in most of the conventional biochemical tests and phenotypically resemble asaccharolytic eubacterium species. the protein profiles of whole cells on sds-page gels and western immunoblotting reacti ... | 2000 | 10758875 |
| antibiotic effects on bacterial profile in osteonecrosis of the jaw. | oral diseases (2011) 18, 85-95 objective: oral infection is considered to play a critical role in the pathogenesis of bisphosphonate-related osteonecrosis of the jaw (bronj), and antibiotic therapy has become a mainstay of bronj therapy. this study was aimed to investigate the effect of antibiotics on bacterial diversity in bronj tissues. materials and methods: the bacterial profile from soft tissues associated with the bronj lesion was determined using 16s rrna-based denaturing gradient gel e ... | 2012 | 21883710 |
| taxonomic characterization of mogibacterium diversum sp. nov. and mogibacterium neglectum sp. nov., isolated from human oral cavities. | novel isolates, strains hm-7, hm-6, hh-31, p9a-ht and ujb13-d, which were isolated from tongue plaque and necrotic dental pulp, were studied taxonomically and phylogenetically. these organisms were anaerobic, non-spore-forming, gram-positive, rod-shaped bacteria that were inert in most of the conventional biochemical tests and phenotypically resemble mogibacterium species or asaccharolytic eubacterium species. the g+c contents of the dnas from the novel isolates ranged from 41 to 42 mol %. dna-d ... | 2002 | 11837293 |
| detection of slackia exigua, mogibacterium timidum and eubacterium saphenum from pulpal and periradicular samples using the polymerase chain reaction (pcr) method. | the purpose of this study was to detect slackia exigua from root canal samples using a sensitive pcr amplification method. mogibacterium timidum and eubacterium saphenum were also included because of their culture-difficult properties. | 2001 | 11556514 |