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purification and properties of l-glutamine and l-asparagine deaminase from pseudomonas aurantiaca ibpm-14.an enzymic preparation of l-glutamine and l-asparagine deamidase was obtained from pseudomonas auractiaca ibpm b-14. the preparation was purified 100--150-fold by thermal treatment and chromotography on columns with biogel p-150 and deae-cellulose. the enzymic activity was measured by the methods of hydroxylaminolysis and direct nesslerization. the deamidase preparation had an activity of 51 i. u. by glutamine and 15 i. e. by asparagine. evidence on the ph effect on the deamidase activity was ac ...197610569
[adsorption of phenylurea derivative and chlorine-substituted aniline herbicides by microorganisms].adsorption of phenylurea derivatives and chlorosubstituted anilines by pseudomonas and aspergillus was studied. all these substances were adsorbed by the microorganisms in large quantities. the bacteria adsorbed more of these substances per biomass unit than the fungi. adsorption of herbicides by the microorganisms is a physical process and is characterized by the same correlation as adsorption of herbicides by soil. derivatives of phenylurea and chlorosubstituted anilines are easily washed from ...1979119144
[3,4-dichloroaniline cometabolism by representatives of the genus pseudomonas].the effect of propanide, linuron and 3,4-dichloroaniline on soil organisms was studied. two strains of pseudomonas aurantiaca 1 and 7, were isolated from soil; they decomposed propanide yielding 3,4-dichloroaniline. these strains, as well as a number of collection cultures belonging to the pseudomonas genus, could transform 3,4-dichloroaniline at a rate of 0-100 per cent during 48 hours. a certain correlation existed between this transformation ability and the level of total oxidase activity. al ...1978651689
[new antibiotically active fluoroglucide from pseudomonas aurantiaca].a new metabolite with an antibiotic activity against grampositive bacteria in concentrations of 0.1--1.0 gamma/ml was isolated from the culture fluid of pseudomonas aurantiaca. the study of its physico-chemical properties showed that it was di-2,4-diacetylfluoroglucylmethan. the conclusion was confirmed by synthetic studies. di-2,4-diacetylfluoroglucylmethan belongs to the group of the antibiotics, derivatives of fluoroglucin, characteristics metabolites of pseudomonas aurantiaca.19751225181
[antigenic polysaccharides of bacteria. 31. the structure of the o-specific polysaccharide chain of pseudomonas aurantiaca imb 31 lipopolysaccharide].the o-specific polysaccharide chain of the pseudomonas aurantiaca imv 31 lipopolysaccharide contains n-acetyl-l-fucosamine (fucnac) and di-n-acetyl-d-bacillosamine (2,4-diacetamido-2,4,6-trideoxyglucose, bac(nac)2) in the ratio 2:1. on the basis of methylation, solvolysis with anhydrous hydrogen fluoride, and computer-assisted analysis of 13c-nmr spectrum, it was concluded that the trisaccharide repeating unit of the polysaccharide possesses the following structure: structure: ----3)-beta-d-bac( ...19882454632
[immunochemical characteristics of a lipopolysaccharide from pseudomonas aurantiaca].a lipopolysaccharide was isolated from pseudomonas aurantiaca imb 31 by extraction with aqueous phenol and purified by ultracentrifugation. the lipopolysaccharide was confined to the phenol phase. fucosamine (2-amino-2,6-dideoxygalactose) (36%) and bacillosamine (2,4-diamino-3,4,6-trideoxyglucose) (23%) were identified as hypothetic components of the o-chain in the carbohydrate moiety of the macromolecule using the techniques of paper chromatography, gas-liquid chromatography and ion-exchange ch ...19883249596
[the nature of functional groups in the active center of antitumor glutamin-(asparagin-)ase].the effect of two reagents on glutamin (asparagin) ase from pseudomonas aurantiaca-548 has been studied. 2,3-butanedione which modified arginine residues was ineffective for the inactivation of the enzyme. the enzyme was completely inactivated in the presence of n-ethyl-5-phenylisoxazolium-3'-sulfonate (woodward's reagent k). the effects of ph, reagent concentration, competitive inhibitors and their analogues on the rate or degree of enzyme inactivation were studied. the experimental results sug ...19883359021
[taxonomic study of pseudomonas aurantiaca nakhimovskaya, 1948 and the proposal of a neotype strain of this species].the type strain of pseudomonas aurantiaca vkm b-876 (ncib 10068) was shown to be identical to p. aureofaciens in its biological properties, g + c--content of dna and chemical nature of produced pigments. its genome relatedness value with the type strain of p. aureofaciens was found to be 82%. the properties of 36 orange-pigmented fluorescent bacterial strains corresponding with the special description of p. aurantiaca, are presented. their dna homology values with other fluorescent species and w ...19853930927
[characteristics of pseudomonas aurantiaca]. 19724643937
[physiological and biochemical characteristics of pseudomonas aurantiaca nachimovskaya, 1948]. 19725043507
[glutamin(asparagin)ase from pseudomonas aurantiaca bkmb-548].a method for isolation of glutamin (asparagin) ase from pseudomonas aurantiaca bkmb-548 has been developed. the enzyme preparation is homogeneous during polyacrylamide gel electrophoresis (ph 7.5 and 7.0) with sds. the ph-optima of the enzyme thermal stability and of the glutaminase activity are equal to 6.0-8.0. at higher ph values the asparaginase activity increases within the ph range of 4-9. the amino acid composition of glutamin(asparagin)ase has been determined.19817248377
[molecular and catalytic properties of bacterial glutamin-(asparagin-)ase].the review summarizes and analyzes experimental evidence for the properties of glutamine(asparagine)ase from pseudomonas aurantiaca-548. the enzyme is a tetramer having a molecular weight of 148 kd and consisting of 4 identical subunits having a molecular weight of 37 kd. for glutaminase activity, the optimum ph is in the range of 6.0-8.0, asparaginase activity increases as ph rises. the enzyme is maximally stable at ph 6.8-8.0. the michaelis constants are 5.3 +/- 0.7 x 10(-6) m for l-glutamine ...19957756933
[an improved method of purification and properties of glutamine asparaginase from pseudomonas aurantiaca 548]. 19979471269
2,5-dialkylresorcinol biosynthesis in pseudomonas aurantiaca: novel head-to-head condensation of two fatty acid-derived precursors.2-hexyl-5-propylresorcinol is the predominant analog of several dialkylresorcinols produced by pseudomonas aurantiaca (pseudomonas fluorescens bl915). we isolated and characterized three biosynthetic genes that encode an acyl carrier protein, a beta-ketoacyl-acyl carrier protein synthase iii, and a protein of unknown function, all of which collectively allow heterologous production of 2-hexyl-5-propylresorcinol in escherichia coli. two regulatory genes exhibiting similarity to members of the ara ...200312533461
[ultrastructure of resting cells of some non-spore-forming bacteria].using electron microscopy (ultrathin sections and freeze-fractures), we investigated the ultrastructure of the resting cells formed in the cultures of micrococcus luteus, arthrobacter globiformis, and pseudomonas aurantiaca under conditions of prolonged incubation (up to 9 months). these resting cells included cyst-like forms that were characterized by complex cell structure and the following ultrastructural properties: (i) a thickened or multiprofiled cell wall (cw), typically made up of a laye ...200415521179
[regulation of 3-desoxy-d-arabino-heptulosonate-7-phosphate synthetase in the bacteria pseudomonas aurantiaca b-162].the regulation of activity and synthesis of dahp-synthase in pseudomonas aurantiaca b-162 was studied. analysis of partially purified preparations of the enzyme revealed two isoenzymes: dahp-synthase [tyr] and dahp-synthase [phe], each of them being regulated by a corresponding amino acid. dahp-synthase [tyr] is a dominant isoenzyme presenting 78 % of the enzyme activity, 50 % inhibition of which is possible by 1,3 x 10(-5) m of tyrosine. dahp-synthase [phe] is minor isoenzyme (sharing 22 % of e ...200516334224
[non-species-specific effects of unacylated homoserine lactone and hexylresorcinol, low molecular weight autoregulators, on the growth and development of bacteria].we conducted a comparative study of the effects of alpha-amino-gamma-butyrolactone, the common structural element of extracellular microbial regulators of the homoserine lactone (hsl) group, and of 4-n-hexylresorcinol, an autoregulator of the alkylhydroxybenzene (ahb) group, on the growth and development of gram-positive and gram-negative bacteria. we revealed non-species-specific effects of hsl and ahb and characterized their concentration dependencies. the addition of 10(-5)-10(-3) m hsl or 10 ...200617025172
fatty acid profiles of dna-bound and whole-cell lipids of pseudomonas aurantiaca drastically differ. 200617286106
reclassification of pseudomonas aurantiaca as a synonym of pseudomonas chlororaphis and proposal of three subspecies, p. chlororaphis subsp. chlororaphis subsp. nov., p. chlororaphis subsp. aureofaciens subsp. nov., comb. nov. and p. chlororaphis subsp. aurantiaca subsp. nov., comb. nov.pseudomonas chlororaphis, pseudomonas aureofaciens and pseudomonas aurantiaca were considered as separate species until 1989, when p. aureofaciens was proposed as a later heterotypic synonym of p. chlororaphis with p. aurantiaca remaining as a separate species. nevertheless, analysis of the almost complete 16s rrna gene sequences revealed that the type strain of p. aurantiaca, ncimb 10068(t), shows gene sequence similarities close to 99.5 % with respect to p. chlororaphis dsm 50083(t) and p. aur ...200717551044
heterologous expression and optimized one-step separation of levansucrase via elastin-like polypeptides tagging system.elastin-like polypeptides (elps) undergo a reversible inverse phase transition upon a change in temperature. this thermally triggered phase transition allows for a simple and rapid means of purifying a fusion protein. recovery of elps-tagged fusion protein was easily achieved by aggregation, triggered either by raising temperature or by adding salt. in this study, levansucrase has been used as a model enzyme in the development of a simple one-step purification method using elps. the levansucrase ...200718092457
characterization of antimicrobial compounds produced by pseudomonas aurantiaca s-1.pseudomonas aurantiaca s-1 can serve as a natural source of pesticides towards phytopathogens like fusarium oxysporum p1 and pseudomonas syringae pv. glycinea bim b-280. the largest pool of produced antimicrobial compounds was found in four days-old spent culture supernatant. at least two groups of bioactive substances were identified, one responsible for the antibacterial activity and the other for the antifungal activity. the fraction with strong antibacterial activity had the molecular mass 2 ...200718254494
[properties of the phenotypic variants of pseudomonas aurantiaca and p. fluorescens].different capacity for phenotypic variation of pseudomonas aurantiaca and p. fluorescens, in populations of cyst-like resting cells (crc), during their germination on solid media, was shown to be a characteristic trait of biodiversity for the dormant forms of these bacteria. this biodiversity manifests itself as qualitative and quantitative differences in the spectra and emergence frequency of phenotype variants, obtained by plating of crc, and depends on the conditions of crc formation and stor ...200819137715
[characteristics of pseudomonas aurantiaca dna supramolecular complexes at various developmental stages].differences in viscoelasticity (eta) and molecular mass (m) values, as well as in the fatty acid profile of lipids in dna supramolecular complexes (sc) isolated from pseudomonas aurantiaca cultures at the exponential and stationary growth phases were established for the first time. typical characteristics of dna sc from actively growing cells were the following: eta = 315 +/- 15 dl/g, m(dna) = 39 x 10(6) da, c16:0 > c18:0 > c18:1 present as basic fatty acids (fa) in a pool of loosely dna-bound l ...200919334598
[cloning of phzir from the endophytic pseudomonas sp. g5 and its expression in escherichia coli].we isolated a new strain of endophytic pseudomonas g5 from the stems of chinese parsley (coriandrum sativum l.), and it is tentatively identified as pseudomonas aurantiaca according to analysis of the entire substrate utilization profiles using biolog microstation system (biolog, inc, hayward ca). an array of evidence established that many gram-negative bacteria employ quorum sensing (qs) system to regulate gene expression in response to cell density using small diffusible signal molecules, n-ac ...200919777809
characterization of a phenazine and hexanoyl homoserine lactone producing pseudomonas aurantiaca strain pb-st2, isolated from sugarcane stem.a novel strain of fluorescent pseudomonad (pb-st2) was isolated from surface-sterilized stems of sugarcane grown in pakistan. the bacterium was identified as pseudomonas aurantiaca on the basis of 16s rrna gene sequence analysis and results from physiological and biochemical characteristics carried out with api50 ch and qts 24 bacterial identification kits. assays using substrate specific media for enzymes revealed lipase and protease activities but cellulase, chitinase, or pectinase were not de ...200920075638
isolation and identification of a pathogen of silkworm bombyx mori.a pathogenic bacterial strain, st-1, was isolated from a naturally infected silkworm. the strain was identified on the basis of its physiological and biochemical properties and the results of sequence analysis of its 16s rrna gene. the results of the 16s rrna gene sequence analysis revealed that st-1 shared the highest sequence identity (more than 99%) with pseudomonas chlororaphis subsp. aurantiaca. st-1 bacteria were gram-negative and 0.7-0.9 × 1.3-1.5 μm long, short rods with rounded ends. th ...201021046395
Levansucrases from Pseudomonas syringae pv. tomato and P. chlororaphis subsp. aurantiaca: Substrate specificity, polymerizing properties and usage of different acceptors for fructosylation.Levansucrases of Pseudomonas syringae pv. tomato DC3000 (Lsc3) and Pseudomonas chlororaphis subsp. aurantiaca (also Pseudomonas aurantiaca) (LscA) have 73% identity of protein sequences, similar substrate specificity and kinetic properties. Both enzymes produce levan and fructooligosaccharides (FOS) of varied length from sucrose, raffinose and sugar beet molasses. A novel high-throughput chip-based nanoelectrospray mass spectrometric method was applied to screen alternative fructosyl acceptors f ...201121820018
nature of dna-bound fatty acids in pseudomonas aurantiaca.the existence of pseudomonas aurantiaca dna-bound fatty acids and lipids is presented in this work. the isolation of dna was carried out by two different procedures, namely, phenol and detergent-based phenol isolation in order to prove the presence of dna-bound lipids. the lipid content of dna is expressed in terms of fatty acid profile. a high level of 16:0, 18:0 and 18:1 is characteristic for tightly bound dna lipids. on the other hand, the fatty acids such as 14:1, iso14:0 and iso16:0 are fou ...200616989655
[effect of alkylhydroxybenzenes, microbial anabiosis inducers, on the structural organization of pseudomonas aurantiaca dna and on phenotypic dissociation induction].we revealed a relationship between alkylhydroxybenzene (ahb)-induced changes in the structural organization of supramolecular complexes (sc) of the dna of pseudomonas auraniaca and the phenotypic dissociation of this bacterium. the addition of 0.1-0.3 mm hexylresorcinol (c6-ahb), a chemical analogue of microbial anabiosis autoinducers, caused the formation of cystlike refractile cells (crc) in these gram-negative, nonsporulating bacteria. inoculating pseudomonad crc on solid nutrient media resul ...200515938390
[inactivation of microbial glutamin-(asparagin-)ase by azaserine and 6-diazo-5-oxo-l-norleucine].the effect of substrate analogues on glutamin-(asparagin-)ase from pseudomonas aurantiaca-548 has been studied. the enzyme was demonstrated to be highly sensitive to the the action of 6-diazo-5-oxo-l-norleucine and azaserine. l-isomers of glutamine, aspartate, glutamate and several other substrate analogues with free alpha-amino groups protected the enzyme against the inhibitory don effect. thus, thorough preliminary selection of appropriate inhibitors, their dosage and treatment duration is nee ...19854074870
6-diazo-5-oxo-l-norleucine and azaserine as affinity inhibitors of glutamin(asparagin)ase.incubation of homogeneous glutamin(asparagin)ase from pseudomonas aurantiaca with 6-diazo-5-oxo-l-norleucine (don) and azaserine leads to an almost complete inactivation of the enzyme. the inactivation process in both cases involves the step of reversible binding of the enzyme with the inhibitor into a complex and subsequent modification of the enzyme within this complex. the data on saturation of the enzyme by low concentrations of inhibitors, the protective effect of substrate and its analogs ...19863707592
[isolation of pseudomonas aurantiaca strains capable of overproduction of phenazine antibiotics].n-methyl-n'-nitro-n-nitrosoguanidine (nh)-induced mutagenesis with subsequent selection for resistance to toxic amino acid analogues (azaserine, m-fluoro-dl-phenylalanine, and 6-diazo-5-oxo-l-norleucine) was applied to pseudomonas aurantiaca b-162. the resulting strains produced phenazine antibiotics three times more efficiently than the wild type strain and ten times more efficiently than the known pseudomonad strains. overproduction of phenazine antibiotics was shown to result either from dere ...200818522322
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