| a new method for the detection of proteolytic activity in pseudomonas lundensis after sodium dodecyl sulfate-polyacrylamide gel electrophoresis. | a new method for the visualization of proteolytic activity in cell culture supernatant from pseudomonas lundensis after sodium dodecyl sulfate (sds)--gel electrophoresis is described. following conventional electrophoresis, the gel is washed in a methanol-containing buffer to facilitate partial removal of sds. after incubation with 0.5% casein the gel is stained for protein with coomassie brilliant blue r-250. bands with proteolytic activity appear as clear areas in the gel against a blue-staine ... | 1995 | 7737090 |
| identification of pseudomonads from fresh and chill-stored chicken carcasses. | results of carbon source assimilation tests (17 carbon compounds) led to 88% of pseudomonads from cold-stored chicken carcasses being assigned to one of 17 groups. of these groups, 13 had combinations of properties identical to, or with readily recognizable degrees of similarity to those of published species/biovars. two of the four groups having carbon assimilation patterns dissimilar to any known species had cellular fatty acid composition corresponding to pseudomonas fluorescens, and two to p ... | 1998 | 9553797 |
| characterization of pseudomonas spp. associated with spoilage of gilt-head sea bream stored under various conditions. | the population dynamics of pseudomonads in gilt-head sea bream mediterranean fish (sparus aurata) stored under different conditions were studied. phenotypic analysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole-cell proteins were performed to identify a total of 106 pseudomonas strains isolated from s. aurata stored under different temperatures (at 0, 10, and 20 degrees c) and packaging conditions (air and a modified atmosphere of 40% co(2)-30% n(2)-30% o(2)). pseudomon ... | 2002 | 11772610 |
| composition of the bacterial population of refrigerated beef, identified with direct 16s rrna gene analysis and pure culture technique. | the composition of the dominating population of freshly cut beef, and beef stored at 4 degrees c for 8 d, was studied by direct analysis of the 16s rrna gene (pcr amplification, cloning and sequencing) and compared with pure culture technique where the isolates picked from the viable plate count were identified by sequencing of the 16s rrna gene. the composition of the bacterial population was recorded at two different time points, at the start when the viable plate count of the meat was 4 x 10( ... | 2007 | 17765348 |
| seasonal influence on heat-resistant proteolytic capacity of pseudomonas lundensis and pseudomonas fragi, predominant milk spoilers isolated from belgian raw milk samples. | psychrotolerant bacteria and their heat-resistant proteases play a major role in the spoilage of uht-processed dairy products. summer and winter raw milk samples were screened for the presence of such bacteria. one hundred and three proteolytic psychrotolerant bacteria were isolated, characterized by api tests, rep-pcr fingerprint analysis and evaluated for heat-resistant protease production. twenty-nine strains (representing 79% of the complete collection) were further identified by 16s rrna ge ... | 2009 | 19196277 |
| regulation of pyrimidine formation in pseudomonas lundensis. | the regulation of pyrimidine formation in the food spoilage agent pseudomonas lundensis atcc 49968 by pyrimidines was examined. in p. lundensis cells grown on glucose as a carbon source, the enzymes aspartate transcarbamoylase, dihydroorotase, and orotidine 5'-monophosphate decarboxylase were induced by orotic acid. pyrimidine auxotrophs containing reduced transcarbamoylase or orotate phosphoribosyltransferase activity were isolated using chemical mutagenesis and selection procedures. independen ... | 2009 | 19370069 |
| heterogeneity of heat-resistant proteases from milk pseudomonas species. | pseudomonas fragi, pseudomonas lundensis and members of the pseudomonas fluorescens group may spoil ultra high temperature (uht) treated milk and dairy products, due to the production of heat-stable proteases in the cold chain of raw milk. since the aprx gene codes for a heat-resistant protease in p. fluorescens, the presence of this gene has also been investigated in other members of the genus. for this purpose an aprx-screening pcr test has been developed. twenty-nine representatives of import ... | 2009 | 19481283 |
| identification of a proteolytic bacterium, hw08, and characterization of its extracellular cold-active alkaline metalloprotease ps5. | a psychrophilic protease-producing bacterium, hw08, was isolated from sediment of the yellow sea in eastern china. on the basis of 16s rdna sequence analysis and physiological properties, the isolate was identified as pseudomonas lundensis. the secreted protease, named ps5, was purified from the culture supernatant as a monomer with an apparent molecular mass of 46 kda on sds-page. as a metalloprotease (inhibited by edta), the enzyme showed maximum activity at 30 degrees c at ph 10.4. it had no ... | 2010 | 20530907 |
| influence of storage conditions on the growth of pseudomonas species in refrigerated raw milk. | the refrigerated storage of raw milk throughout the dairy chain prior to heat treatment creates selective conditions for growth of psychrotolerant bacteria. these bacteria, mainly belonging to the genus pseudomonas, are capable of producing thermoresistant extracellular proteases and lipases, which can cause spoilage and structural defects in pasteurized and ultra-high-temperature-treated milk (products). to map the influence of refrigerated storage on the growth of these pseudomonads, milk samp ... | 2010 | 21115713 |
| impact of hydrocarbons, pcbs and heavy metals on bacterial communities in lerma river, salamanca, mexico: investigation of hydrocarbon degradation potential. | freshwater contamination usually comes from runoff water or direct wastewater discharges to the environment. this paper presents a case study which reveals the impact of these types of contamination on the sediment bacterial population. a small stretch of lerma river basin, heavily impacted by industrial activities and urban wastewater release, was studied. due to industrial inputs, the sediments are characterized by strong hydrocarbon concentrations, ranging from 2 935 to 28 430μg·kg(-1) of tot ... | 2015 | 25828406 |
| differential effect on cell-mediated immunity in human volunteers after intake of different lactobacilli. | probiotics are live microorganisms which have beneficial effects on the host when ingested in adequate amounts. probiotic bacteria may stimulate immune effector functions in a strain-specific manner. in this blind placebo-controlled trial, we investigated the effects on the immune system following daily intake of six different strains of lactobacilli or the gram-negative bacterium pseudomonas lundensis for 2 or 5 weeks. blood lymphocyte subsets were quantified by fluorescence activated cell sort ... | 2013 | 23574328 |
| pseudomonas endophytica sp. nov., isolated from stem tissue of solanum tuberosum l. in spain. | a bacterial strain named bstt44(t) was isolated in the course of a study of endophytic bacteria occurring in stems and roots of potato growing in a soil from salamanca, spain. the 16s rrna gene sequence had 99.7% identity with respect to that of its closest relative, pseudomonas psychrophila e-3t, and the next most closely related type strains were those of pseudomonas fragi, with 99.6% similarity, pseudomonas deceptionensis, with 99.2% similarity, and pseudomonas lundensis, with 99.0% similarit ... | 2015 | 25851593 |
| biofilm formation characteristics of pseudomonas lundensis isolated from meat. | biofilms formations of spoilage and pathogenic bacteria on food or food contact surfaces have attracted increasing attention. these events may lead to a higher risk of food spoilage and foodborne disease transmission. while pseudomonas lundensis is one of the most important bacteria that cause spoilage in chilled meat, its capability for biofilm formation has been seldom reported. here, we investigated biofilm formation characteristics of p. lundensis mainly by using crystal violet staining, and ... | 2015 | 26551486 |