| hypertonic sodium bicarbonate for taxus media-induced cardiac toxicity in swine. | to determine whether intravenous (iv) hypertonic sodium bicarbonate is effective in the reversal of qrs widening associated with severe taxus intoxication. | 2002 | 11874772 |
| [an intron-free methyl jasmonate inducible geranylgeranyl diphosphate synthase gene from taxus media and its functional identification in yeast]. | geranylgeranyl diphosphate synthase (ggpps, ec: 2.5.1.29) catalyzes the biosynthesis of geranylgeranyl diphosphate (ggpp), which is a key precursor for diterpenes including taxol, one of the most potent antitumor drugs. in order to investigate the role of ggpp synthase in taxol biosynthesis, we cloned, characterized and functionally expressed the ggpp synthase gene from taxus media. a 3743-bp genomic sequence of t. media was isolated by genome walking strategy which contained an 1182-bp open rea ... | 2005 | 15773543 |
| identification of two thaumatin-like proteins (tlps) in the pollination drop of hybrid yew that may play a role in pathogen defence during pollen collection. | we describe the proteomic identification of two pathogenesis-related group 5 (pr-5) proteins, an acidic thaumatin-like protein (tlp) and a basic tlp isolated from the pollination drop of hybrid yew (taxus x media rehder). the basic tlp (txmtlpb) was the most abundant protein in the yew pollination drop based on protein spot size after two-dimensional electrophoresis. the acidic tlp (txmtlpa) is also a major protein component of the yew ovular secretion and appears to be encoded by a number of mr ... | 2007 | 17938097 |
| an endophytic taxol-producing fungus from taxus x media, aspergillus candidus md3. | an endophytic taxol-producing fungus (strain md3) isolated from the inner bark of taxus x media was identified as aspergillus candidus according to its morphological characteristics, physiological and biochemical characteristics, and 18s rrna gene sequence analysis. taxol produced by a. candidus md3 was shown to be identical to authentic taxol analyzed by uv, hplc, ms and nuclear magnetic resonance spectroscopy. the gene encoding the 10-deacetylbaccatin iii-10-o-acetyl transferase, which catalyz ... | 2009 | 19239498 |
| effect of seven tricyclic diterpenoids from needles of taxus media var. hicksii on stimulus-induced superoxide generation, tyrosyl or serine/threonine phosphorylation and translocation of cytosolic compounds to the cell membrane in human neutrophils. | taxol has been widely used as an anticancer drug for ovarian, breast, lung and prostate cancer. some kinds of taxus plants are widely distributed in the northeast asia region. we have isolated seven tricyclic diterpenoids, taxinine, taxagifine, 5-o-cinnamoyltaxacin i triacetate, 5-decinnamoyltaxinine j, 5-cinnamoyl-9-acetyltaxicin i, taxacin and taxol from the needles of taxus media var. hicksii, and investigated their effects on stimulus-induced superoxide generation and translocation of cytoso ... | 2009 | 19288401 |
| an endophytic taxol-producing fungus from taxus media, cladosporium cladosporioides md2. | fermentation processes using taxol-producing fungi other than taxus spp. may be an alternative way to produce taxol, which is an important antitumor agent used widely in the clinic setting. in this study, a taxol-producing endophytic fungus strain md2 was isolated from the inner bark of taxus media. strain md2 produced taxol when grown in potato dextrose liquid medium. the fungal taxol-which was analyzed by ultraviolet, high-performance liquid chromatography and mass spectrometry-was shown to be ... | 2009 | 19484305 |
| metabolic responses of taxus media transformed cell cultures to the addition of methyl jasmonate. | dedifferentiated taxus media cell cultures presenting the same genetic characteristics as the parent culture were established from transformed roots. two transformed cell lines were studied: rol c, carrying the t-dna of a. rhizogenes 9,402 and txs, carrying both the t-dna of a. rhizogenes and the txs transgene of t. baccata under the control of the 35s camv promoter. in the second part of a previously optimized two-stage system, the transformed cell lines were cultured in a production medium sup ... | 2010 | 20730769 |
| 7,7?-Dimethoxyagastisflavone-induced Apoptotic or Autophagic Cell Death in Different Cancer Cells. | 7,7?-Dimethoxyagastisflavone (DMGF), a biflavonoid isolated from the needles of Taxus × media cv. Hicksii, was evaluated for its antiproliferative and antineoplastic effects in three human cancer cell lines. Interestingly, DMGF caused cell death via different pathways in different cancer cells. DMGF induced apoptosis, activated caspase-3 activity and changed the mitochondrial membrane potential in HT-29 human colon cancer cells. However, the apoptotic pathway is not the major pathway involved in ... | 2011 | 21915931 |
| diversity of endophytic fungi and screening of fungal paclitaxel producer from anglojap yew, taxus x media. | endophytic fungi represent underexplored resource of novel lead compounds and have a capacity to produce diverse class of plant secondary metabolites. here we investigated endophytic fungi diversity and screening of paclitaxel-producing fungi from taxus x media. | 2013 | 23537181 |
| overproduction of geranylgeraniol in coprinopsis cinerea by the expression of geranylgeranyl diphosphate synthase gene. | (e, e, e)-geranylgeraniol (ggoh) is a valuable ingredient of many perfumes and a valuable precursor for synthesizing pharmaceuticals. in an attempt to increase the ggoh concentration in coprinopsis cinerea, we demonstrated that the expression of geranylgeranyl diphosphate synthase (ggpps) gene isolated from taxus x media could promote ggoh production. furthermore, the concentrations of squalene and ergosterol were measured in the engineered strains. expectedly, significant decreases of squalene ... | 2014 | 25138463 |
| [cloning and functional characterization of a cdna encoding isopentenyl diphosphate isomerase involved in taxol biosynthesis in taxus media]. | taxol is one of the most potent anti-cancer agents, which is extracted from the plants of taxus species. isopentenyl diphosphate isomerase (ipi) catalyzes the reversible transformation between ipp and dmapp, both of which are the general 5-carbon precursors for taxol biosynthesis. in the present study, a new gene encoding ipi was cloned from taxus media (namely tmipi with the genbank accession number kp970677) for the first time. the full-length cdna of tmipi was 1 232 bps encoding a polypeptide ... | 2015 | 26234147 |
| coronatine, a more powerful elicitor for inducing taxane biosynthesis in taxus media cell cultures than methyl jasmonate. | coronatine is a toxin produced by the pathogen pseudomonas syringae. this compound has received much attention recently for its potential to act as a plant growth regulator and elicitor of plant secondary metabolism. to gain more insight into the mechanism by which elicitors can affect the biosynthesis of paclitaxel (px) and related taxanes, the effect of coronatine (cor) and methyl jasmonate (meja) on taxus media cell cultures has been studied. for this study, a two-stage cell culture was estab ... | 2013 | 23102875 |
| plant species differences in particulate matter accumulation on leaf surfaces. | particulate matter (pm) accumulation on leaves of 22 trees and 25 shrubs was examined in test fields in norway and poland. leaf pm in different particle size fractions (pm(10), pm(2.5), pm(0.2)) differed among the species, by 10- to 15-folds at both test sites. pinus mugo and pinus sylvestris, taxus media and taxus baccata, stephanandra incisa and betula pendula were efficient species in capturing pm. less efficient species were acer platanoides, prunus avium and tilia cordata. differences among ... | 2012 | 22554531 |
| transforming echoes into pseudo-action potentials for classifying plants. | animals perceive their environment by converting sensory stimuli into action potentials, or temporal point processes, that are interpreted by the brain. this paper investigates the information content of point processes extracted from echoes from in situ plants in an effort to understand how bats recognize landmarks in the field. a mobile sonar converts echoes into biologically similar temporal point processes. termed pseudo-action potentials (paps), whose inter-pap interval relates to echo ampl ... | 2001 | 11681396 |
| glucosylation of the peptide leucinostatin a, produced by an endophytic fungus of european yew, may protect the host from leucinostatin toxicity. | yew species (taxus spp.) throughout the world are hosts to hundreds, or perhaps thousands, of endophytic organisms. most commonly, these organisms are fungi, living in a commensal or a symbiotic relationship with their host plant, so the plants exhibit little or no outward evidence that they are supporting these microorganisms. little is known about any of the biochemical mechanisms that mediate the interactions between the yew host and its associated microbes. we feel that such information may ... | 1997 | 9263641 |
| evaluation of selected plants for acute toxicosis in budgerigars. | pairs of budgerigars were given samples, by gavage, of plants considered potentially toxic to pet birds. samples were prepared by flash-freezing and powdering fresh plant material in liquid nitrogen and resuspending the material in deionized water for administration. of the 19 plants tested, only 6 induced clinical signs of illness; these plants included yew, oleander, clematis, avocado, black locust, and virginia creeper (taxus media, nerium oleander, clematis sp, persea americana, robinia pseu ... | 1992 | 1577644 |
| molecular cloning and heterologous expression of a 10-deacetylbaccatin iii-10-o-acetyl transferase cdna from taxus x media. | a full-length cdna encoding 10-deacetylbaccatin iii-10-o-acetyl transferase (designated as tmdbat), which catalyzes the acetylation of the c-10 hydroxyl group of the advanced metabolite 10-deacetylbaccatin iii (10-dab) to yield baccatin iii, the immediate diterpenoid precursor of taxol, was isolated from taxus x media. heterologous expression of tmdbat in e. coli demonstrated that tmdbat was a functional gene. tissue expression pattern analysis revealed that tmdbat expressed strongly in leaves, ... | 2007 | 17094009 |