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fungal endophytes of vanilla planifolia across réunion island: isolation, distribution and biotransformation.the objective of the work was to characterize fungal endophytes from aerial parts of vanilla planifolia. also, to establish their biotransformation abilities of flavor-related metabolites. this was done in order to find a potential role of endophytes on vanilla flavors.201526070309
species delimitation in fungal endophyte diversity studies and its implications in ecological and biogeographic inferences.the estimation of species diversity in fungal endophyte communities is based either on species counts or on the assignment of operational taxonomic units (otus). consequently, the application of different species recognition criteria affects not only diversity estimates but also the ecological hypotheses that arise from those observations. the main objective of the study was to examine how the choice and number of genetic markers and species delimitation criteria influence biodiversity estimates ...201121557783
endophytic taxol-producing fungi from bald cypress, taxodium distichum.pestalotiopsis microspora occurs as a range of strains in bald cypress, taxodium distichum. the organisms live as endophytes in the bark, phloem and xylem, and isolates show differences in cultural and microscopic characteristics on common laboratory media. many of these fungi make taxol as determined by the reactivity of partially purified culture extracts with specific monoclonal antibodies against taxol. in the case of one strain of p. microspora (cp-4), taxol was isolated from culture medium ...19968760934
induction of the sexual stage of pestalotiopsis microspora, a taxol-producing fungus.pestalotiopsis microspora, isolate ne-32, is an endophyte of the himalayan yew (taxus wallichiana) that produces taxol, an important chemotherapeutic drug used in the treatment of breast and ovarian cancers. conditions were determined to induce the perfect stage (teleomorph) of this organism in the laboratory as a critical first step to study inheritance of taxol biosynthetic genes. the perfect stage of pestalotiopsis microspora ne-32 forms in a period of 3-6 weeks on water agarose with dried ye ...200010931912
isopestacin, an isobenzofuranone from pestalotiopsis microspora, possessing antifungal and antioxidant activities.isopestacin is an isobenzofuranone obtained from the endophytic fungus pestalotiopsis microspora. while a few other isobenzofuranones are known from natural sources, isopestacin is the only one having a substituted benzene ring attached at the c-3 position of the furanone ring. the compound was isolated from culture broths of the fungus and crystallized and its structure was determined by x-ray crystallography. both proton and carbon nmr spectral assignments are also reported for isopestacin. th ...200212009322
biodegradation of polyester polyurethane by endophytic fungi.bioremediation is an important approach to waste reduction that relies on biological processes to break down a variety of pollutants. this is made possible by the vast metabolic diversity of the microbial world. to explore this diversity for the breakdown of plastic, we screened several dozen endophytic fungi for their ability to degrade the synthetic polymer polyester polyurethane (pur). several organisms demonstrated the ability to efficiently degrade pur in both solid and liquid suspensions. ...201121764951
multihydroxylation of ursolic acid by pestalotiopsis microspora isolated from the medicinal plant huperzia serrata.the structural modification of ursolic acid by an endophytic fungus pestalotiopsis microspora, isolated from medicinal plant huperzia serrata was reported for the first time. the structure diversity was very important for the sar study of ursolic acid and its derivatives. incubation of ursolic acid 1 with p. microspora afforded four metabolites: 3-oxo-15α, 30-dihydroxy-urs-12-en-28-oic acid (2), 3β, 15α-dihydroxy-urs-12-en-28-oic acid (3), 3β, 15α, 30- trihydroxy-urs-12-en-28-oic acid (4) and 3, ...201121767617
a putative histone deacetylase modulates the biosynthesis of pestalotiollide b and conidiation in pestalotiopsis microspora.fungi of the genus pestalotiopsis have drawn attention for their capability to produce an array of bioactive secondary metabolites that have potential for drug development. here, we report the determination of a polyketide derivative compound, pestalotiollide b, in the culture of the saprophytic fungus pestalotiopsis microspora nk17. structural information acquired by analyses with a set of spectroscopic and chromatographic techniques suggests that pestalotiollide b has the same skeleton as the ...201525394605
melanin is required for the formation of the multi-cellular conidia in the endophytic fungus pestalotiopsis microspora.melanin plays an important role in regulating various biological processes in many fungi. however, its biological role in conidiation remains largely elusive. we report here that conidia production, morphogenesis, integrity, germination and their viability in pestalotiopsis microspora require the polyketide-derived melanin. a polyketide synthase gene, pks1, was identified and demonstrated responsible for melanin biosynthesis in this fungus. a targeted deletion mutant strain δpks1 displayed a def ...201526411889
microsporols a-c from the plant endophytic fungus pestalotiopsis microspore.three new ambuic acid derivatives, microsporols a-c (1-3) and the known compound ambuic acid (4), were isolated from the solid-substrate fermentation cultures of the plant endophytic fungus pestalotiopsis microspora. their structures were elucidated primarily by nmr experiments. the absolute configurations of the 6,7-diol moiety in 1 and 2 were assigned using the snatzke's method, whereas that of 3 was deduced by circular dichroism (cd) exciton chirality method. compounds 1, 3, and 4 showed mode ...201526669093
a b-type histone acetyltransferase hat1 regulates secondary metabolism, conidiation, and cell wall integrity in the taxol-producing fungus pestalotiopsis microspora.in filamentous fungi, many gene clusters for the biosynthesis of secondary metabolites often stay silent under laboratory culture conditions because of the absence of communication with its natural environment. epigenetic processes have been demonstrated to be critical in the expression of the genes or gene clusters. here, we report the identification of a b-type histone acetyltransferase, hat1, and demonstrate its significant roles in secondary metabolism, conidiation, and the cell wall integri ...201627400176
cytotoxic 14-membered macrolides from a mangrove-derived endophytic fungus, pestalotiopsis microspora.seven new 14-membered macrolides, pestalotioprolides c (2), d-h (4-8), and 7-o-methylnigrosporolide (3), together with four known analogues, pestalotioprolide b (1), seiricuprolide (9), nigrosporolide (10), and 4,7-dihydroxy-13-tetradeca-2,5,8-trienolide (11), were isolated from the mangrove-derived endophytic fungus pestalotiopsis microspora. their structures were elucidated by analysis of nmr and ms data and by comparison with literature data. single-crystal x-ray diffraction analysis was used ...201627556865
biogenesis of silver nanoparticles using endophytic fungus pestalotiopsis microspora and evaluation of their antioxidant and anticancer activities.an endophytic fungal strain isolated from the leaves of gymnema sylvestre was identified as pestalotiopsis microspora vj1/vs1 based on nucleotide sequencing of internal transcribed spacer region (its 1-5.8s-its 2) of 18s rrna gene (ncbi accession number kx213894). in this study, an efficient and ecofriendly approach has been reported for the synthesis of silver nanoparticles (agnps) using aqueous culture filtrate of p. microspora. ultraviolet-visible analysis confirmed the synthesis of agnps by ...201727826190
improved pestalotiollide b production by deleting competing polyketide synthase genes in pestalotiopsis microspora.pestalotiollide b, an analog of dibenzodioxocinones which are inhibitors of cholesterol ester transfer proteins, is produced by pestalotiopsis microspora nk17. to increase the production of pestalotiollide b, we attempted to eliminate competing polyketide products by deleting the genes responsible for their biosynthesis. we successfully deleted 41 out of 48 putative polyketide synthases (pkss) in the genome of nk17. nine of the 41 pks deleted strains had significant increased production of pesta ...201728005187
taxol from pestalotiopsis microspora, an endophytic fungus of taxus wallachiana.pestalotiopsis microspora was isolated from the inner bark of a small limb of himalayan yew, taxus wallachiana, and was shown to produce taxol in mycelial culture. taxol was identified by spectroscopic and chromatographic comparisons with authentic taxol. optimal taxol production occurred after 2-3 weeks in still culture at 23 degrees c. [14c]acetate and [14c]phenylalanine served as precursors for fungal [14c]taxol. these observations on p. microspora are discussed in relation to the biological ...19968932715
the relationship between an endangered north american tree and an endophytic fungus.the florida torreya (torreya taxifolia) began a catastrophic decline in the late 1950s and is now the rarest tree in north america for which a full species designation has been established. the trees have common plant disease symptoms, but the reason for the decline has never been identified. t. taxifolia's imminent extinction gains special poignancy through its close relationship to the pacific yew (taxus brevifolia), which produces the potent anticancer agent, taxol.19959383479
analysis of beta-tubulin cdnas from taxol-resistant pestalotiopsis microspora and taxol-sensitive pythium ultimum and comparison of the taxol-binding properties of their products.the anti-cancer drug taxol binds to beta-tubulin in assembled microtubules and causes cell cycle arrest in animal cells; in contrast, in fungi, the effect of taxol varies. for instance, the taxol-producer pestalotiopsis microspora ne32, an ascomycete, is resistant to taxol (ic50 greater than 11.7 microm), whereas pythium ultimum, an oomycete, is sensitive to taxol (ic50 0.1 microm). in order to understand the differential fungal response to taxol, we isolated cdnas encoding beta-tubulin from bot ...199910628871
bioprospecting highly diverse endophytic pestalotiopsis spp. with antibacterial properties from maytenus ilicifolia, a medicinal plant from brazil.thirteen endophytic fungal strains of the genus pestalotiopsis were isolated from the medicinal plant maytenus ilicifolia mart. ex. reiss (commonly known as "espinheira santa") and their antimicrobial properties were investigated. two isolates were successful in inhibiting the growth of the tested microorganisms (escherichia coli, klebsiella pneumoniae, micrococcus luteus, staphylococcus aureus, and methicillin-resistant staphylococcus aureus (mrsa)) using the technique of bioautographic thin-la ...200718026204
in vivo addition of telomeric repeats to foreign dna generates extrachromosomal dnas in the taxol-producing fungus pestalotiopsis microspora.transformation of the taxol-producing filamentous fungus pestalotiopsis microspora with a plasmid containing the bacterial hygromycin resistance gene fused to aspergillus regulatory sequences resulted in the in vivo formation of extrachromosomal dnas with telomeric repeats in the majority of transformants. repeats of the telomeric sequence 5'-ttaggg-3' were appended to nontelomeric transforming dna termini. no fungal sequences other than telomeric repeats were detected in extrachromosomal dnas. ...19989756714
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