phlebotomine sandfly species in paraguay and their infection with leishmania.nine species of sandflies, lutzomyia (nyssomyia) whitmani (antunes and countinho), lutzomyia (nyssomyia) intermedia (lutz and neiva), lutzomyia (psathyromyia) shannoni (dyar), lutzomyia migonei (franca), lutzomyia (pintomyia) fischeri (pinto), lutzomyia (pintomyia) pessoai (countinho and barretto), lutzomyia cortelezzii (brethes), lutzomyia walkeri (newstead) and lutzomyia (trichopygomyia) longispinus (mangabeira), were caught, by human bait and shannon trap, in four areas of paraguay hyper-ende ...19921417209
lutzomyia migonei (frança 1920) naturally infected with peripylarian flagellates in baturité, a focus of cutaneous leishmaniasis in ceará state, brazil. 19902152202
[ecologic aspects of american tegumentary leishmaniasis. 5. stratification of the spatial and seasonal activities of phlebotominae (diptera, psychodidae) in areas of agricultural culture of the vale do ribeira region, state of são paulo, brazil].this paper deals with the investigation on the composition and stratification of the phebotominae activity developed in patch and clear areas of high agricultural activity. the employment of a cdc light trap proved to be a useful tool in determining the local faune. thirteen species of sandflies and a total of 5,423 individuals were caught. the catch places situated in the ecotone were the ones in which the results reached their best, being outstanding for psychodopygus intermedius, lutzomyia mi ...19873507916
[phlebotomines in an area of transmission of tegumentar leishmaniasis in the northern region of parana state, brazil: seasonal variation and nocturnal activity].in two years of phlebotomine captures in a modified forest an appreciable decrease in the size of this insect population has been observed when compared to previous data from the same locality. the captures with the shannon light trap were made in the outlying forest of palmital farm in terra boa county, parana state, brazil. the predominant species were lutzomyia withmani, lutzomyia migonei, lutzomyia intermedius e lutzomyia fischeri. these insects were active from 6 p.m. to 6 a.m. and the peri ...19938115833
the sand fly fauna (diptera: psychodidae: phlebotominae) of a focus of cutaneous leishmaniasis in ilhéus, state of bahia, brazil.the municipality of ilhéus, state of bahia, has a focus of cutaneous leishmaniasis where entomological studies were carried out to determine the sand fly species and their habits. lutzomyia migonei, l. sallesi, l. tupynambai, l. schreiberi, l. intermedia, l. whitmani, l. yuilli yuilli, l. fischeri, l. pessoai, l. shannoni and l. misionensis were identified. lutzomyia whitmani was the predominant species. specimens were collected indoors, at peridomestic sites, in the cocoa plantations and in oth ...19968734952
contribution to the sand fly fauna (diptera: phlebotominae) of rio grande do sul, brazil and leishmania (viannia) infections.american cutaneous leishmaniasis (acl) is endemic in the state of rio grande do sul, brazil. as reports of nearby hospitals suggest, the parque estadual do turvo seems to serve as a source for the disease. during three months from november 1996 to february 1997 we collected, in this park, 2,228 sandflies (10 lutzomyia species and 2 6species). we applied the polymerase chain reaction to 920 females which belong to the following species: lutzomyia migonei, lu. pessoai, lu. fischeri, lu. misionensi ...199910464396
[impact of environmental changes on sand fly ecology in southern brazil].tree felling around a chicken coop and periodic insecticide spraying at the palmital farm in the county of terra boa in southern brazil impacted the ecology of sand fly species. the most prevalent species (51.6%) before clearing the trees and insecticide spraying was lutzomyia migonei (frança, 1920), and it is now lutzomyia (nyssomyia) whitmani (antunes & coutinho, 1939) (89.8%). there was a highly significant difference between the proportions of l. whitmani reported by this paper (89.8%) and a ...199910633213
deltamethrin-impregnated dog collars have a potent anti-feeding and insecticidal effect on lutzomyia longipalpis and lutzomyia migonei.deltamethrin-impregnated pvc dog collars were tested to assess if they were effective in protecting dogs from sand fly bites of lutzomyia longipalpis and lu. migonei. a protective effect against old world species phlebotomus perniciosus was demonstrated before. four dogs wearing deltamethrin collars and three dogs wearing untreated collars (not impregnated with deltamethrin) were kept in separate kennels for over eight months in a village on the outskirts of fortaleza in ceará, brazil. periodica ...200111562713
assessment of pcr in the detection of leishmania spp in experimentally infected individual phlebotomine sandflies (diptera: psychodidae: phlebotominae).dna amplification by the polymerase chain reaction (pcr) was applied in the investigation of the presence of leishmania (kinetoplastida: trypanosomatidae) parasites in single phlebotomine sandflies. three phlebotomine/parasite pairs were used: lutzomyia longipalpis/leishmania chagasi, lutzomyia migonei/leishmania amazonensis and lutzomyia migonei/leishmania braziliensis, all of them incriminated in the transmission of visceral or cutaneous leishmaniasis. dna extraction was performed with whole i ...200212436164
influence of vertebrate blood meals on the development of leishmania (viannia) braziliensis and leishmania (leishmania) amazonensis in the sand fly lutzomyia migonei (diptera: psychodidae).the effect of blood meals from humans and seven domestic, wild, or laboratory animals (dogs, horses, chickens, rats, opossums, mice, and hamsters) on the development of leishmania braziliensis and l. amazonensis was studied in the sand fly lutzomyia migonei. the development of l. braziliensis and l. amazonensis exhibited peripylarian and suprapylarian patterns of development, respectively, in the sand fly gut with all blood meals tested. the blood meal sources influenced the infection rate of th ...200212518856
lutzomyia vectors for cutaneous leishmaniasis in southern brazil: ecological niche models, predicted geographic distributions, and climate change effects.geographic and ecological distributions of three lutzomyia sand flies that are cutaneous leishmaniasis vectors in south america were analysed using ecological niche modelling. this new tool provides a large-scale perspective on species' geographic distributions, ecological and historical factors determining them, and their potential for change with expected environmental changes. as a first step, the ability of this technique to predict geographic distributions of the three species was tested st ...200312906876
spatial and temporal patterns of phlebotomine sand flies (diptera: psychodidae) in a cutaneous leishmaniasis focus in northern argentina.phlebotomine sand flies (diptera: psychodidae) were captured in an area of argentina endemic for american cutaneous leishmaniasis (acl). a total of 44,944 flies were collected during a 130-wk interepidemic period from 1990 through 1993. these sand flies included lutzomyia neivai (pinto) (97.8%), lutzomyia migonei (franca) (1.2%), lutzomyia cortelezzii (brèthes) (0.8%), lutzomyia shannoni (dyar) (0.1%), and lutzomyia punctigeniculata (floch and abonnenc) (0.1%). lutzomyia neivai was more abundant ...200414989343
development of leishmania (viannia) braziliensis and leishmania (leishmania) amazonensis in the sand fly lutzomyia migonei (diptera: psychodidae).development of leishmania braziliensis (vianna) and leishmania amazonensis (lainson and shaw) in the sand fly lutzomyia migonei (frança) was compared by studying the parasite microhabitats in the alimentary tract, the sequence of parasite morphological changes leading to the metacyclogenesis process, and the parasite transmission to the vertebrate susceptible host. although the infections by the 2 leishmania species were initiated with the same number of amastigotes, le. amazonensis developed a ...200015218917
identification of naturally infected lutzomyia intermedia and lutzomyia migonei with leishmania (viannia) braziliensis in rio de janeiro (brazil) revealed by a pcr multiplex non-isotopic hybridisation identify naturally infected lutzomyia spp. by leishmania (viannia) braziliensis, a pcr multiplex non-isotopic hybridisation assay was developed for the analysis of insect samples collected in distinct areas of the municipality of rio de janeiro (brazil), from march to december 2003. data from experimental infection indicate that the method can detect one individual infected insect out of ten. wild sand flies were classified and grouped into pools of 10 specimens each, reaching a total of 40 f ...200516143358
phlebotominae sand flies associated with a tegumentary leishmaniasis outbreak, tucumán province, argentina.the distribution of sand flies and cases of tegumentary leishmaniasis in the area surrounding jb alberd city, and the proximities of catamarca province were studied, after an increase of reported cases from jb alberdi, tucumán province, in 2003. of 14 confirmed cases, 57% were females and 57% were less than 15 years old, suggesting peridomestic transmission. however, 86% of them lived close to the marapa river forest gallery and related wooded areas. over 1,013 sand flies were collected; lutzomy ...200617119748
phlebotomine sandfly species from an american visceral leishmaniasis area in the northern rainforest region of pernambuco state, brazil.the aim of this study was to identify the fauna of phlebotomine sandflies in an area with incidence of american visceral leishmaniasis (avl) in são vicente férrer, a municipality (county) located in the northern zona da mata in pernambuco state, brazil. sandfly captures were conducted monthly for four nights/month from december 2002 to november 2003, by means of manual captures (with a shannon trap) and by cdc light traps. a total of 23,156 specimens of phlebotomines sandflies were collected in ...200717486244
leishmania chagasi: homogenous metacyclic promastigotes isolated by buoyant density are highly virulent in a mouse model.homogenous metacyclic promastigotes of leishmania chagasi were isolated by buoyant density from in vitro heterogeneous cultures and used for biochemical characterization of isoforms of the major surface protease (msp). compared to stationary phase promastigotes, metacyclic cells had three times more msp, produced 3-fold higher parasite loads in a mouse model in vivo, and were more resistant to complement-mediated lysis in vitro. these metacyclic l. chagasi expressed both the virulence-associated ...200817706646
urban distribution of phlebotominae in a cutaneous leishmaniasis focus, argentina.urbanization and vector domestication are currently proposed as factors that contributed to the recent increase of american cutaneous leishmaniasis (acl). is likely also urban transmission? oran is the main city in the argentinean hyper-endemic area of acl, and human cases in urban residences are usually reported. in order to assess the spatial distribution of risk, phlebotomine traps were located in different environments of oran. a total of 7,787 sand flies were captured: lutzomyia neivai (98. ...200818545855
establishment of a large semi-field system for experimental study of african malaria vector ecology and control in tanzania.medical entomologists increasingly recognize that the ability to make inferences between laboratory experiments of vector biology and epidemiological trends observed in the field is hindered by a conceptual and methodological gap occurring between these approaches which prevents hypothesis-driven empirical research from being conducted on relatively large and environmentally realistic scales. the development of semi-field systems (sfs) has been proposed as the best mechanism for bridging this ga ...200818715508
phlebotominae (diptera: psycodidae) fauna in the chaco region and cutaneous leishmaniasis transmission patterns in argentina, the incidence of american cutaneous leishmaniasis (acl) has shown a steady increase over the last few decades. in the chaco biogeographical region, specifically, several outbreaks of acl were recently reported in addition to the usual time-space scattering of acl cases. however, little is known about the sandfly composition in the eastern, humid chaco (hc) region or the western, dry chaco (dc) region. therefore, phlebotomine captures were performed throughout this region and an ana ...200818949328
studies on the sandfly fauna (diptera: psychodidae: phlebotominae) from transmission areas of american cutaneous leishmaniasis in state of acre, brazil.studies were undertaken on the phlebotomines in the municipalities of bujari, xapuri and rio branco in the state of acre. the abundance of species on the ground and in the tree canopy was estimated by standardized index of species abundance. of the 52 species identified, lutzomyia (n.) antunesi, lutzomyia (n.) whitmani, lutzomyia (p.) davisi, lutzomyia migonei, lutzomyia (n.) umbratilis, lutzomyia (n.) flaviscutellata, lutzomyia (t.) ubiqui-talis, lutzomyia (p.) hirsuta hirsuta, lutzomyia (p.) p ...200819148413
canine leishmaniosis in south america.abstract : canine leishmaniosis is widespread in south america, where a number of leishmania species have been isolated or molecularly characterised from dogs. most cases of canine leishmaniosis are caused by leishmania infantum (syn. leishmania chagasi) and leishmania braziliensis. the only well-established vector of leishmania parasites to dogs in south america is lutzomyia longipalpis, the main vector of l. infantum, but many other phlebotomine sandfly species might be involved. for quite som ...200919426440
[sandflies in an areas with occurrences of human cases of american cutaneous leishmaniasis in the municipality of seropédica, state of rio de janeiro].american cutaneous leishmaniasis is caused by protozoa of the genus leishmania, which affects the skin and mucosa. it is primarily a zoonotic infection. sandfly surveys were conducted in four areas with occurrences of human cases of american cutaneous leishmaniasis in the municipality of seropédica. a manual method was used to catch specimens between 6:00 pm and 10:00 pm, for 12 consecutive months. from october 2004 to september 2005, we caught 2,390 individuals belonging to four species: lutzom ...200919448931
differences in human macrophage receptor usage, lysosomal fusion kinetics and survival between logarithmic and metacyclic leishmania infantum chagasi promastigotes.the obligate intracellular protozoan, leishmania infantum chagasi (lic) undergoes receptor-mediated phagocytosis by macrophages followed by a transient delay in phagolysosome maturation. we found differences in the pathway through which virulent lic metacyclic promastigotes or avirulent logarithmic promastigotes are phagocytosed by human monocyte-derived macrophages (mdms). both logarithmic and metacyclic promastigotes entered mdms through a compartment lined by the third complement receptor (cr ...200919702651
lutzomyia migonei as putative vector of visceral leishmaniasis in la banda, argentina.four autochthonous cases of human visceral leishmaniasis (vl) were reported in la banda, santiago del estero from june 2007 to may 2008. in the vicinity of these cases there were 3/47 rk39 sero-positive dogs, and another 4 dogs with vl were reported by passive surveillance. the sero-positive dogs and infected humans lived within a 3.1 km radius. phebotomine sand fly captures were performed twice during november/december 2007 and april 2008. in 20 of the 59 sampled sites in the areas of the human ...201019716797
differential midgut attachment of leishmania (viannia) braziliensis in the sand flies lutzomyia (nyssomyia) whitmani and lutzomyia (nyssomyia) intermedia.the interaction between leishmania and sand flies has been demonstrated in many old and new world species. besides the morphological differentiation from procyclic to infective metacyclic promastigotes, the parasite undergoes biochemical transformations in its major surface lipophosphoglycan (lpg). an upregulation of beta-glucose residues was previously shown in the lpg repeat units from procyclic to metacyclic phase in leishmania (viannia) braziliensis, which has not been reported in any leishm ...201020011070
chicken blood provides a suitable meal for the sand fly lutzomyia longipalpis and does not inhibit leishmania development in the gut.abstract:201020205803
using recombinant proteins from lutzomyia longipalpis saliva to estimate human vector exposure in visceral leishmaniasis endemic areas.leishmania is transmitted by female sand flies and deposited together with saliva, which contains a vast repertoire of pharmacologically active molecules that contribute to the establishment of the infection. the exposure to vector saliva induces an immune response against its components that can be used as a marker of exposure to the vector. performing large-scale serological studies to detect vector exposure has been limited by the difficulty in obtaining sand fly saliva. here, we validate the ...201020351785
distribution of phlebotomine sand flies (diptera: psychodidae) in a primary forest-crop interface, salta, argentina.disordered urbanization and deforestation are the main activities proposed as causal factors of re-emergence of american cutaneous leishmaniasis caused by leishmania braziliensis. the purpose of this work was to investigate, in the hyperendemic area of argentina, the distribution of phlebotomine sand flies at the modified primary vegetation-crop interface, as one of the potential sites where the effects of changing landscape on sand fly populations may be manifested. twenty samplings were made b ...201021175047
[repellent activity of plant essential oils against bites of lutzomyia migonei (diptera: psychodidae)].natural repellents from plant extracts have demonstrated good efficacy against bites of some insect species. the present study evaluated the repellent effect of essential oils extracted from 8 plants species against bites of lutzomyia migonei, the leishmania vector. the essential oils were extracted by steam destillation in clevenger chamber, from the following plants: hyptis suaveolens, pimenta racemosa, piper marginatum, monticalia imbricatifolia, pseudognaphalium caeruleocanum, espeletia shul ...201021250485
the salivary glands of two sand fly vectors of leishmania: lutzomyia migonei (fran+ºa) and lutzomyia ovallesi (ortiz)(diptera: psychodidae).leishmaniasis is a vector-borne disease transmitted by the intradermal inoculation of leishmania (kinetoplastida: trypanosomatidae) promastigotes together with saliva during the bite of an infected sand fly.201021713342
sybr green-based real-time pcr targeting kinetoplast dna can be used to discriminate between the main etiologic agents of brazilian cutaneous and visceral leishmaniases.abstract: background: leishmaniases control has been hampered by the unavailability of rapid detection methods and the lack of suitable therapeutic and prophylactic measures. accurate diagnosis, which can distinguish between leishmania isolates, is essential for conducting appropriate prognosis, therapy and epidemiology. molecular methods are currently being employed to detect leishmania infection and categorize the parasites up to genus, complex or species level. real-time pcr offers several a ...201222240199
subversion of complement by hematophagous parasites.the complement system is a crucial part of innate and adaptive immunity which exerts a significant evolutionary pressure on pathogens. it has selected for those pathogens, mainly microorganisms but also parasites, that have evolved countermeasures. the characterization of how pathogens evade complement attack is a rapidly developing field of current research. in recent years, multiple complement evasion strategies have been characterized. in this review, we focus on complement escape mechanisms ...200918762211
proceedings of the ix international symposium on phlebotomine sandflies (isops ix), reims, france, june 28th-july 1st, 2016. 201627677701
diversity and distribution of sandflies (diptera: psychodidae: phlebotominae) in a military area in the state of amazonas, brazil.this study reports the distribution, ecotopes and fauna diversity of sandflies captured in five training bases on a military reserve in manaus, state of amazonas (am). a total of 10,762 specimens were collected, which were distributed among 58 species, with the highest number recorded at base instruction 1 (bi1). a higher rate of species richness was found at the base instruction boina rajada and low levels of diversity associated with a high abundance index with the clear dominance of lutzomyia ...023903983
sand-fly saliva-leishmania-man: the trigger trio.leishmaniases are worldwide diseases transmitted to the vertebrate host by the bite of an infected sand-fly. sand-fly biting and parasite inoculation are accompanied by the injection of salivary molecules, whose immunomodulatory properties are actively being studied. this mini review focuses on how the interactions between sand-fly saliva and the immune system may shape the outcome of infection, given its immunomodulatory properties, in experimental models and in the endemic area. additionally, ...201324312093
multi-modal analysis of courtship behaviour in the old world leishmaniasis vector phlebotomus argentipes.the sand fly phlebotomus argentipes is arguably the most important vector of leishmaniasis worldwide. as there is no vaccine against the parasites that cause leishmaniasis, disease prevention focuses on control of the insect vector. understanding reproductive behaviour will be essential to controlling populations of p. argentipes, and developing new strategies for reducing leishmaniasis transmission. through statistical analysis of male-female interactions, this study provides a detailed descrip ...201425474027
current and future niche of north and central american sand flies (diptera: psychodidae) in climate change scenarios.ecological niche models are useful tools to infer potential spatial and temporal distributions in vector species and to measure epidemiological risk for infectious diseases such as the leishmaniases. the ecological niche of 28 north and central american sand fly species, including those with epidemiological relevance, can be used to analyze the vector's ecology and its association with transmission risk, and plan integrated regional vector surveillance and control programs. in this study, we mod ...201324069478
lipophosphoglycans from leishmania amazonensis strains display immunomodulatory properties via tlr4 and do not affect sand fly infection.the immunomodulatory properties of lipophosphoglycans (lpg) from new world species of leishmania have been assessed in leishmania infantum and leishmania braziliensis, the causative agents of visceral and cutaneous leishmaniasis, respectively. this glycoconjugate is highly polymorphic among species with variation in sugars that branch off the conserved gal(β1,4)man(α1)-po4 backbone of repeat units. here, the immunomodulatory activity of lpgs from leishmania amazonensis, the causative agent of di ...201627508930
aspects of the ecology of phlebotomines (diptera: psychodidae: phlebotominae) in an area of cutaneous leishmaniasis occurrence, municipality of angra dos reis, coast of rio de janeiro state, brazil.over a complete two-year period, phlebotomine specimens were caught in an area of cutaneous leishmaniasis occurrence in the municipality of angra dos reis. a manual suction tube was used to catch phlebotomines on house walls, and also light traps in domestic and peridomestic settings and in the forest. this yielded 14,170 specimens of 13 species: two in the genus brumptomyia and eleven in the genus lutzomyia. l. intermedia predominantly in domestic and peridomestic settings, with little presence ...024626417
acoustic communication in insect disease vectors.acoustic signalling has been extensively studied in insect species, which has led to a better understanding of sexual communication, sexual selection and modes of speciation. the significance of acoustic signals for a blood-sucking insect was first reported in the xix century by christopher johnston, studying the hearing organs of mosquitoes, but has received relatively little attention in other disease vectors until recently. acoustic signals are often associated with mating behaviour and sexua ...024473800
environmental niche modelling of phlebotomine sand flies and cutaneous leishmaniasis identifies lutzomyia intermedia as the main vector species in southeastern brazil.cutaneous leishmaniasis (cl) is caused by a protozoan of the genus leishmania and is transmitted by sand flies. the state of espírito santo (es), an endemic area in southeast brazil, has shown a considerably high prevalence in recent decades. environmental niche modelling (enm) is a useful tool for predicting potential disease risk. in this study, enm was applied to sand fly species and cl cases in es to identify the principal vector and risk areas of the disease. sand flies were collected in 46 ...201627783641
bacterial diversity of the american sand fly lutzomyia intermedia using high-throughput metagenomic sequencing.parasites of the genus leishmania cause a broad spectrum of diseases, collectively known as leishmaniasis, in humans worldwide. american cutaneous leishmaniasis is a neglected disease transmitted by sand fly vectors including lutzomyia intermedia, a proven vector. the female sand fly can acquire or deliver leishmania spp. parasites while feeding on a blood meal, which is required for nutrition, egg development and survival. the microbiota composition and abundance varies by food source, life sta ...201627581188
phenotypic and genotypic variations among three allopatric populations of lutzomyia umbratilis, main vector of leishmania south america, lutzomyia umbratilis is the main vector of leishmania guyanensis, one of the species involved in the transmission of american tegumentary leishmaniasis. in brazil, l. umbratilis has been recorded in the amazon region, and in the state of pernambuco, northeastern region, where an isolated population has been identified. this study assessed the phylogeographic structure and size and shape differences of the wing of three brazilian populations.201526338469
molecular detection of leishmania in phlebotomine sand flies in a cutaneous and visceral leishmaniasis endemic area in northeastern brazil.several phlebotomine sand fly species have been regarded as putative or proven vectors of parasites of the genus leishmania in brazil, but data for the northeastern region remains incipient. in this study, a total of 600 phlebotomine sand flies were grouped in pools of 10 specimens each and tested by a leishmania genus-specific pcr and by a pcr targeting leishmania (leishmania) infantum. fourteen out of 60 pools were positive by the genus-specific pcr, being five pools of l. migonei, seven of l. ...025076439
peridomiciliary breeding sites of phlebotomine sand flies (diptera: psychodidae) in an endemic area of american cutaneous leishmaniasis in southeastern brazil.the occurrence of american cutaneous leishmaniasis (acl) in areas modified by humans indicates that phlebotomine sand fly vectors breed close to human habitations. potential peridomiciliary breeding sites of phlebotomines were sampled in an area of transmission of leishmania (viannia) braziliensis in southeastern brazil. three concentric circles rounding houses and domestic animal shelters, with radii of 20, 40, and 60 m, defined the area to be monitored using adult emergence traps. of the 67 ph ...201223091196
distribution and abundance of phlebotominae, vectors of leishmaniasis, in argentina: spatial and temporal analysis at different scales.the spatial-temporal analysis of the abundance of insects, vectors of tegumentary leishmaniasis (tl) and visceral leishmaniasis (vl), was performed in argentina using spatial-temporal increasing scales. in the microscale (microfocal), the effect of the primary vegetation-crop interface in vector abundance was observed, and also how the shelters, food sources, and other environmental characteristics contribute to habitat microheterogeneity and so to a microheterogeneous vector distribution. in th ...201222315620
acoustic signals in the sand fly lutzomyia (nyssomyia) intermedia (diptera: psychodidae).acoustic signals are part of the courtship of many insects and they often act as species-specific signals that are important in the reproductive isolation of closely related species. here we report the courtship songs of the sand fly lutzomyia (nyssomyia) intermedia, one of the main vectors of cutaneous leishmaniasis in brazil.201121569534
leishmaniasis transmission in an ecotourism area: potential vectors in ilha grande, rio de janeiro state, brazil.the south coast of rio de janeiro state, in brazil, is endemic for cutaneous and visceral leishmaniases and is frequently visited by tourists from different parts of the world. since the complex epidemiology of leishmaniases demands local studies, the goal of this study was to investigate the phlebotomine sand fly fauna and leishmaniases transmission in ilha grande, an ecotourism area of angra dos reis municipality.201324499568
identification of phlebotomine sand fly blood meals by real-time pcr.phlebotomine sand flies are blood-feeding insects of great medical and veterinary significance acting as vectors of leishmania parasites. studying the blood-feeding pattern of these insects may help in the understanding of their interactions with potential reservoir hosts of leishmania parasites. in this study, we developed real time pcr assays for the identification of sand fly blood meal.201525889289
participation of ticks in the infectious cycle of canine visceral leishmaniasis, in teresina, piauí, this study, we detected leishmania spp. infection in r. sanguineus collected from dogs that were naturally infected with l. (l.) infantum. we examined 35 dogs of both sexes and unknown ages. the infected dogs were serologically positive by the immunofluorescence antibody test (ifat), enzyme-linked immunosorbent assay (elisa), and quick test-dpp (dual path platform), as well as parasitological examination of a positive skin biopsy or sternal bone marrow aspiration. ten negative dogs were inclu ...025076429
could phlebotomus mascittii play a role as a natural vector for leishmania infantum? new data.the occurrence of phlebotomine sand flies in central europe was questioned until they were recorded for the first time in germany in 1999, and ten years later also in austria. the aim of this study was to investigate sand flies collected in austria for their carrier status of leishmania spp.201627542911
the effect of avian blood on leishmania development in phlebotomus duboscqi.the development of pathogens transmitted by haematophagous invertebrate vectors is closely connected with the digestion of bloodmeals and is thus affected by midgut enzymatic activity. some studies have demonstrated that avian blood inhibits leishmania major infection in the old world vector phlebotomus papatasi; however, this effect has never been observed in the new world vectors of the genus lutzomyia infected by other leishmania species. therefore, our study was focused on the effect of chic ...201324059328
a lectin from dioclea violacea interacts with midgut surface of lutzomyia migonei, unlike its homologues, cratylia floribunda lectin and canavalia gladiata lectin.leishmaniasis is a vector-borne disease transmitted by phlebotomine sand fly. susceptibility and refractoriness to leishmania depend on the outcome of multiple interactions that take place within the sand fly gut. promastigote attachment to sand fly midgut epithelium is essential to avoid being excreted together with the digested blood meal. promastigote and gut sand fly surface glycans are important ligands in this attachment. the purpose of the present study was to evaluate the interaction of ...201425431778
visceral leishmaniasis caused by leishmania infantum in salta, argentina: possible reservoirs and vectors.cases of human visceral leishmaniasis (hvl) were not recorded until recently in the chaco region of northwestern argentina. dogs were surveyed at the sites of infection of two hvl index cases in the chaco region of salta province. canine cases (canl) were diagnosed by two parasitological methods, two molecular methods targeting mini- and maxicircle dna, and immunochromatographic dipstick. among 77 dogs studied, 10 (13%) were found infected with leishmania spp. in seven dogs and two humans, the i ...201526055744
canine visceral leishmaniasis case investigation in the jacare region of niteroi, rio de janeiro, brazil.american visceral leishmaniasis is a vector-borne zoonosis in expansion in brazil. dogs are the main urban reservoir. departing from a case of canine visceral leishmaniasis (cvl) in jacaré, niterói, rio de janeiro state, an epidemiological canine and entomological study was performed to assess the extension of the disease at the location. sample was collected around the case and the dogs identified by serological tests (rapid double platform immunochromatographic exams, immunoenzymatic assay/eli ...026422157
lutzomyia migonei is a permissive vector competent for leishmania infantum.leishmania infantum is the most widespread etiological agent of visceral leishmaniasis (vl) in the world, with significant mortality rates in human cases. in latin america, this parasite is primarily transmitted by lutzomyia longipalpis, but the role of lutzomyia migonei as a potential vector for this protozoan has been discussed. laboratory and field investigations have contributed to this hypothesis; however, proof of the vector competence of l. migonei has not yet been provided. in this study ...201626988559
molecular identification of lutzomyia migonei (diptera: psychodidae) as a potential vector for leishmania infantum (kinetoplastida: trypanosomatidae).visceral leishmaniasis (vl) in brazil is caused by the protozoan leishmania infantum. this parasite is transmitted by the bite of a female sand fly. the most important sand fly species in vl transmission is lutzomyia longipalpis. in fortaleza, the capital of ceará state, brazil, the simultaneous occurrence of lutzomyia migonei and l. longipalpis was detected in localities where vl transmission is observed. the purpose of this study was to determine conclusively if l. migonei can be found natural ...201626995718
ecological aspects of phlebotomine sand flies (diptera, psychodidae, phlebotominae) in areas of american cutaneous leishmaniasis, in the municipality of paraty, rio de janeiro, brazil. i-index of abundance by location and type of capture.the description of the first and autochthonous case of diffuse cutaneous leishmaniasis in the municipality of paraty, state of rio de janeiro, brought the interest of this study. sand flies were captured over a 3-yr period. using manual suction tubes, sand flies were collected from the inner and outer walls of homes, in the living spaces of domestic animals, and in shannon light traps, which were set up outside homes and in the forest. cdc light traps were installed inside homes, around the exte ...201526336245
ecology of lutzomyia longipalpis and lutzomyia migonei in an endemic area for visceral leishmaniasis.the main vector for visceral leishmaniasis (vl) in brazil is lutzomyia longipalpis. however, the absence of l. longipalpis in a region of autochthonous vl demonstrates the participation of other species in the transmission of the parasite. studies conducted in la banda, argentina, and são vicente férrer, pernambuco state, brazil, have correlated the absence of l. longipalpis and the presence of l. migonei with autochthonous cases of vl. in são vicente férrer, pernambuco, there was evidence for t ...201625271451
sandflies (diptera: psychodidae) in an urban area of northeastern brazil.the sandfly fauna is well studied globally. in brazil, sandfly fauna is very diverse in the northeast region, especially in states such as maranhão, ceará, and bahia. however, in the state of pernambuco, the distribution of these insects is still not well known. therefore, the objective of this study was to identify the different species that constitute the sandfly fauna in an urban area in the northeast region of brazil, where an outbreak of visceral leishmaniasis (vl) was recently reported.201728001216
ecological and epidemiological aspects of the sand fly (diptera, psychodidae) fauna of the national monument of pontões capixabas, state of espírito santo, southeastern brazil.we evaluated the ecological and epidemiological aspects of the sand fly fauna in an area of the atlantic forest biome with records of visceral and cutaneous leishmaniasis. sand fly collections at three different localities at the national monument of pontões capixabas, state of espírito santo, southeastern brazil, were conducted by using two centers of disease control and prevention automatic light traps in the peridomiciliary environment and eight centers of disease control and prevention autom ...201324843925
study of sand flies (diptera: psychodidae) in visceral and cutaneous leishmaniasis areas in the central-western state of minas gerais, brazil.the transmission of leishmania involves several species of sand flies that are closely associated with various parasites and reservoirs, with differing transmission cycles in brazil. a study on the phlebotomine species composition has been conducted in the municipality of divinópolis, minas gerais, brazil, an endemic area for cutaneous leishmaniasis (cl), which has intense occurrence of visceral leishmaniasis (vl) cases. in order to study the sand flies populations and their seasonality, cdc lig ...201323178219
studies on phlebotominae (diptera: psychodidae) in the campus fiocruz mata atlântica, jacarepaguá, in the city of rio de janeiro, brazil.the presence of american cutaneous leishmaniasis (acl) in the communities of the campus fiocruz mata atlântica (cfma) in the city of rio de janeiro initiated the investigation of the phlebotominae fauna in the atlantic forest to determine the occurrence of putative acl vectors associated with the enzootic cycle.201525860460
[sand flies in timóteo, minas gerais, brazil]sporadic cases of tegumentary leishmaniasis have occurred in timóteo, minas gerais state, basically among the rural population. in order to study the region's sand fly population, new jersey light traps were set in seven different neighborhoods. specimens were gathered from june through october 1994, december 1994, and january through march 1995, with a total of 3,240 hours per trap. a total of 4,396 sand flies were captured, distributed among two genera and twenty species: brumptomyia cunhai, b ...199710886917
insecticide activity of ageratina jahnii and ageratina pichinchensis (asteraceae) against lutzomyia migonei (diptera: psychodidae).insects are mostly pathogens transmitters, thus the necessity of finding effective bioinsecticides to combat them. in the present investigation, the insecticide activity of ageratina jahnii and ageratina pichinchensis (asteraceae) essential oils, methanol, and aqueous extracts was evaluated against lutzomyia migonei (diptera: psychodidae) females, leishmania transmitters, a wide distributed parasitosis in latin america.028553626
lipophosphoglycan polymorphisms do not affect leishmania amazonensis development in the permissive vectors lutzomyia migonei and lutzomyia longipalpis.lipophosphoglycan (lpg) is a dominant surface molecule of leishmania promastigotes. its species-specific polymorphisms are found mainly in the sugars that branch off the conserved gal(β1,4)man(α1)-po4 backbone of repeat units. leishmania amazonensis is one of the most important species causing human cutaneous leishmaniasis in the new world. here, we describe lpg intraspecific polymorphisms in two le. amazonensis reference strains and their role during the development in three sand fly species.201729246180
copulatory courtship song in lutzomyia migonei (diptera: psychodidae).lutzomyia migonei is a vector of leishmaniasis with a wide distribution in south america, which could favour population differentiation and speciation. cryptic species of the lutzomyia longipalpis complex, the widely distributed sand fly vector of visceral leishmaniasis in latin america, have previously been shown to display distinct copulation songs. we found that lu. migonei males also produce a song during copulation. this "lovesong" presents short trains (6-8 pulses) with an inter-pulse inte ...201021225208
adulticide effect of monticalia greenmaniana (asteraceae) against lutzomyia migonei (diptera: psychodidae).leishmaniasis is a public health problem that has been increasing year by year, with the further difficulty that an efficient control system is not available. therefore, it is necessary to search for less contaminating and dangerous alternatives for controlling leishmania transmitting sandflies. the purpose of this study was to evaluate the activity of monticalia greenmaniana (asteraceae) extracts and essential oil as an adulticide against lutzomyia migonei (diptera: psychodidae) females, from a ...201222476600
phlebotomine sandflies (diptera: psychodidae) in são vicente férrer, a sympatric area to cutaneous and visceral leishmaniasis in the state of pernambuco, the last decades, a considerable geographic expansion of the leishmaniases in all regions of brazil has been observed. the present study was carried out to identify the composition of the phlebotomine sandfly fauna and verify the seasonal variation of the main species after environmental changes occurred in são vicente férrer municipality, state of pernambuco, brazil.201222370831
hourly activity of lutzomyia neivai in the endemic zone of cutaneous leishmaniasis in tucumán, argentina: preliminary the present work, the hourly activity of lutzomyia neivai was studied in the southern part of the province of tucumán, argentina, in an area of transmission of cutaneous leishmaniasis during two months of higher activity. in addition, the variables that influenced the abundance of lu. neivai were evaluated. a total of 1,146 individuals belonging to lu. neivai (97%) and lutzomyia migonei (3%) were captured. the hourly activity of lu. neivai was mainly nocturnal, with a bimodal pattern in both ...201121894389
comparative study of the mouthparts of males and females of lutzomyia migonei (diptera: psychodidae) by scanning electron microscopy.the mouthparts of both males and females of lutzomyia migonei frança were compared by scanning electron microscopy (sem) and light microscopy (lm), and their structures are herein described. mandibles are present in both sexes but in males they are reduced and, in contrast to females, no teeth were observed. as in females, the laciniae in males are highly sclerotized, but the number of lateral teeth are reduced and retrorse teeth are absent. only females possess teeth at the tip of the hypophary ...200011004789
physical factors influencing the oviposition of lutzomyia migonei (diptera: psychodidae) in laboratory determine the influence of physical factors on oviposition of lutzomyia migonei (diptera: psychodidae) under laboratory conditions, two sets of experiments were performed. the first test was to determine the influence of the size of pots on oviposition. gravid flies were placed individually or in groups in different oviposition pots. the number of eggs laid, oviposition time and survival of gravid females were observed. in the second experiment, the influence of irregular surfaces on oviposit ...20069566207
comparison of three methods for age-grading of female neotropical phlebotomine sandflies.changes in appearance of the accessory glands (ag), ovaries (ov) and the absence or presence of crease marks at the edges of the genital atrium armature (ga) were compared as methods to age-grade female laboratory-bred (lutzomyia migonei) and wild-caught (lu.youngi, lu.spinicrassa, lu.ovallesi) neotropical sandflies. dissections at different physiological stages revealed that all parous and high proportions of nulliparous (4/38) unmated, 12/12 mated) females showed similar residual ag secretions ...19979061671
the genital atrium as a new character for distinguishing parous and nulliparous neotropical sandflies.changes in the appearance of the membrane delimiting the genital atrium of lutzomyia migonei are described for the first time. dissections of laboratory-bred females at different physiological stages (i.e. on emergence, after a bloodmeal and after oviposition) revealed clear and constant differences between the membranes of the parous and nulliparous flies. the state of the membrane can be used as an alternative method for accurately age-grading female sandflies. dissection methods and a simplif ...19968762411
[american cutaneous leishmaniasis: phlebotominae of the area of transmission in the north of paraná, brazil].most of the cases of cutaneous leishmaniasis diagnosed in the laboratory of the state university of maringá, paraná, brazil, are related to areas of northern paraná state, which have been settled since 40 years age, when the destructions of vast areas of forest in this region was begun. the native vegetation, already, in large part, destroyed is constituted of dense forest of the type found in the transition from tropical to subtropical regions. the landscape is constituted of plateau with a cli ...19911784969
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