| evaluation of the genus listonella and reassignment of listonella damsela (love et al.) macdonell and colwell to the genus photobacterium as photobacterium damsela comb. nov. with an emended description. | the genus listonella, which was recently described on the basis of 5s rrna sequence data, was found to be of dubious value on the basis of the results of a comparison of a number of taxonomic studies involving members of the vibrionaceae. the available data suggest that 5s rrna sequences may be of limited taxonomic use at the intra- and intergeneric levels, at least for apparently recently evolved groups, such as the vibrionaceae. in this light, we assessed the generic assignment of the species ... | 1991 | 1742198 |
| nasitrema sp.-associated encephalitis in a striped dolphin (stenella coeruleoalba) stranded in the gulf of mexico. | an immature female striped dolphin (stenella coeruleoalba) found dead on a northwestern florida beach in 1988 exhibited severe inflammation bilaterally in the dorsal and mid-thalamus in association with adult trematodes (nasitrema sp.) and trematode eggs. numerous specimens of nasitrema sp. also were present in the pterygoid sinuses. pneumonia in association with a heavy growth of vibrio damsela was observed also. this report confirms the occurrence of nasitrema sp.-associated encephalitis in st ... | 1991 | 1758040 |
| identification of vibrio vulnificus o serovars with antilipopolysaccharide monoclonal antibody. | a serotyping scheme for vibrio vulnificus predicated on the detection of lipopolysaccharide (lps) antigens is proposed. the serovar o typing scheme used to type v. vulnificus employs polyclonal antisera raised in rabbits immunized with heat-killed whole-cell vaccines. polyclonal typing sera produced in this manner cross-react with heterologous strains. affinity purification of polyclonal antisera with lps affinity columns resolved some of these cross-reactions; however, affinity-purified polyclo ... | 1991 | 1761690 |
| cloning and expression of the damselysin gene from vibrio damsela. | the gene encoding damselysin, an extracellular cytolysin produced by virulent vibrio damsela strains, was cloned and expressed in escherichia coli. dna sequences homologous to that of the cloned gene were detected in hemolytic strains of v. damsela but not in other hemolytic vibrio species. | 1990 | 2294056 |
| phospholipase d activity of vibrio damsela cytolysin and its interaction with sheep erythrocytes. | exposure of sheep erythrocytes to sublytic amounts of vibrio damsela cytolysin markedly reduced their membrane sphingomyelin content and their sensitivity to lysis by the sphingomyelin-dependent cytolysins staphylococcal sphingomyelinase c (beta-toxin) and helianthin. the toxin was found to be a phospholipase d active against sphingomyelin. | 1987 | 2890587 |
| vibrio damsela: another potentially virulent marine vibrio. | | 1986 | 3950452 |
| purification and characterization of an extracellular cytolysin produced by vibrio damsela. | large amounts of an extremely potent extracellular cytolysin produced by the halophilic bacterium vibrio damsela were obtained free of detectable contamination with medium constituents and other bacterial products by sequential ammonium sulfate precipitation, gel filtration with sephadex g-100, and hydrophobic interaction chromatography with phenyl-sepharose cl-4b. the cytolysin is heat labile and protease sensitive and has a molecular weight (estimated by sodium dodecyl sulfate-polyacrylamide g ... | 1985 | 4008049 |
| illness caused by vibrio damsela and vibrio hollisae. | vibrio damsela was isolated from six wound infections in otherwise healthy persons. in five of the six cases the wounds were known to have been exposed to salt or brackish water at the time of the injury. vibrio hollisae was isolated from an index stool culture in nine cases in which no other enteric pathogen was identified. all nine patients had diarrhoea and abdominal pain; one patient had bloody diarrhoea. six of the nine patients were known to have eaten raw seafood in the five days before t ... | 1982 | 6123029 |
| halophilic vibrio species from seafish in senegal. | sucrose-positive and sucrose-negative halophilic vibrio species at counts of up to 10(7)/100 g were isolated from muscles tissue in 27 and 43%, respectively, of 128 seafish from coastal waters in senegal. vibrio parahaemolyticus, including 21% urease-positive strains, was the most common isolate, followed by vibrio alginolyticus, vibrio vulnificus, vibrio damsela, and vibrio fluvialis. | 1984 | 6476829 |
| cytolytic activity and virulence of vibrio damsela. | a correlation was observed between the ability of 19 isolates of vibrio damsela, a halophilic bacterium recently recognized as a human pathogen, to cause disease in mice and to produce large amounts of a cytolytic toxin in vitro. the yield of toxin in the culture supernatant fluids was optimal during the mid- and late-logarithmic phases of growth in medium containing 0.5% na+ ion, was stable during the stationary growth phase, and was significantly reduced in culture medium containing greater th ... | 1984 | 6715036 |
| role of iron in the pathogenicity of vibrio damsela for fish and mammals. | the ability to obtain iron of 14 isolates of vibrio damsela with different degrees of virulence for mice and turbot (scophthalmus maximus) has been evaluated in artificial and natural iron-restricted environments. all strains were capable of utilizing haemoglobin (hb) and ferric ammonium citrate (fac) as the sole iron sources in vitro. however, only virulent v. damsela strains were able to resist the bacteriostatic and bactericidal effects of human and turbot sera, their growth being enhanced by ... | 1994 | 7926669 |
| vibrio damsela. a cause of fulminant septicemia. | a previously healthy 70-year-old man presented with a rapidly progressive and fulminant infection due to vibrio damsela after suffering a knife cut while filleting bluefish at the new jersey shore. despite appropriate antibiotic therapy and localized wound exploration, the patient died. to our knowledge, this is the first reported case of v damsela sepsis with simultaneous isolation of the organism from both blood and wound. we are reporting this case to heighten physicians' awareness of this in ... | 1993 | 8333818 |
| primary vibrio damsela septicemia. | | 1996 | 8722948 |
| vibrio damsela associated with diseased fish in denmark. | a total of 26 vibrio damsela strains were isolated in denmark. fifteen strains were isolated from the head kidney of rainbow trout (oncorhynchus mykiss) in aquaculture, eight were from organs of two stingrays (dasyatis pastinaca) held in captivity, two were from organs and pathological material of a nurse shark (orectolobus ornatus) held in captivity, and one was from the water in the aquarium where the rays and shark were kept. this is the first report on the isolation of v. damsela in denmark ... | 1997 | 9293023 |
| rapidly fatal infection due to photobacterium (vibrio) damsela. | | 1997 | 9356821 |
| misidentification of unusual aeromonas species as members of the genus vibrio: a continuing problem. | two unusual cases of aeromonas infection are described, one associated with bacteremia (aeromonas schubertii) and another in which the organism was recovered from an infected gall bladder (aeromonas veronii biotype veronii). these strains were initially identified as vibrio damsela and vibrio cholerae by the vitek and api 20e systems, respectively. use of appropriate screening tests and familiarity with the newer aeromonas species could prevent initial misidentifications and potential public hea ... | 1998 | 9542946 |
| influence of the sequence of administration of beta-glucans and a vibrio damsela vaccine on the immune response of turbot (scophthalmus maximus l.). | yeast (saccharomyces cerevisae) beta-1,3 glucans were used as adjuvant in a vibrio damsela vaccine for turbot (scophthalmus maximus l.). turbot were injected with the adjuvant prior, at the same time and after the vaccine. several immune parameters (index and rate of phagocytosis, passive haemolytic plaque numbers, and agglutinating antibody titers) were determined at different times postinoculation. the highest activity of all the immune parameters was obtained when glucans were injected after ... | 1998 | 9656431 |
| filamentous bacteriophages of vibrio parahaemolyticus as a possible clue to genetic transmission. | we have previously reported the isolation and characterization of two filamentous bacteriophages of vibrio parahaemolyticus, designated vf12 and vf33. in this study, to understand the potential of these phages as tools for genetic transmission, we investigated the gene structures of replicative-form (rf) dnas of their genomes and the distribution of these dnas on chromosomal and extrachromosomal dnas. the 7,965-bp nucleotide sequences of vf12 and vf33 were determined. an analysis of the overall ... | 1998 | 9748441 |
| characterization of porins isolated from the outer membrane of vibrio damsela. | a protein oligomer with an approximate molecular weight of its 37-kda monomer form was purified from the cell envelope fraction of vibrio damsela cells. this oligomer exhibited strong porin activity when reconstituted into proteoliposomes with phosphatidyl choline. the functional properties for the 37-kda protein suggest that it is a nonspecific or general porin, with an apparent pore size of 1.6 nm. this porin allows penetration of a variety of hydrophilic solutes according to their molecular m ... | 1999 | 9841774 |
| 16s rrna gene sequence analysis of photobacterium damselae and nested pcr method for rapid detection of the causative agent of fish pasteurellosis. | the causative agent of fish pasteurellosis, the organism formerly known as pasteurella piscicida, has been reclassified as photobacterium damselae subsp. piscicida on the basis of 16s rrna gene sequence comparisons and chromosomal dna-dna hybridization data; thus, this organism belongs to the same species as photobacterium damselae subsp. damselae (formerly vibrio damselae). since reassignment of p. damselae subsp. piscicida was based on only two strains, one objective of the present work was to ... | 1999 | 10388687 |
| a study on the prevalence of gram-negative bacteria in bulk tank milk. | bulk tank milk from 131 dairy herds in eastern south dakota and western minnesota were examined for coliforms and noncoliform bacteria. coliforms were detected in 62.3% of bulk tank milk samples. counts ranged from 0 to 4.7 log10 cfu/ml. the mean count was 3.4 log10 cfu/ml. gram-negative noncoliform bacteria were observed in 76.3% of bulk tank milk. counts ranged from 0 to 6.2 log10 cfu/ml. the mean count was 4.8 log10 cfu/ml. a total of 234 isolates from bulk tank milk were examined to species ... | 1999 | 10629809 |
| presence of phospholipase-d (dly) gene coding for damselysin production is not a pre-requisite for pathogenicity in photobacterium damselae subsp. damselae. | the presence of the phospholipase-d (dly) gene as pre-requisite for virulence of photobacterium damselae subsp. damselae for poikilotherm and homoiotherm animals was investigated in a total of 17 strains isolated from fish, shellfish, mammals and seawater. with this aim, we developed two pcr protocols. a simple pcr using primers flanking the almost complete dly gene, and a multiplex-pcr using two sets of primers directed to internal fragments of the dly and 16s rrna genes. only six of the 17 ph. ... | 2000 | 10644497 |
| photobacterium histaminum okuzumi et al. 1994 is a later subjective synonym of photobacterium damselae subsp. damselae (love et al. 1981) smith et al. 1991. | the type strain of photobacterium histaminum, jcm 8968t (= atcc 51805t), and that of photobacterium damselae subsp. damselae, atcc 33539t, exhibit 100% identity in their 16s rrna sequence, more than 80% dna-dna homology and only one phenotypic difference. also, like p. histaminum, p. damselae subsp. damselae was shown to excrete a large amount of histamine when cells were grown on medium containing excessive histidine under acidic conditions. therefore, the name p. histaminum should be considere ... | 2000 | 10843080 |
| neuraminidase-producing ability of intestinal bacteria isolated from coastal fish. | the neuraminidase-producing ability of bacteria from coastal fish intestines and their environments was determined using a fluorogenic substrate, 2'-(4-methylumbelliferyl)-alpha-d-n-acetylneuraminic acid. of 836 isolates examined, 758 (90.7%) produced little or no neuraminidase (< 0.0002 u ml-1) while 78 (9.3%) produced >/= 0.0002 u neuraminidase ml-1. of note, 10% of vibrios from fish intestines could produce neuraminidases, and 9% did it efficiently. this result suggests that the vibrios capab ... | 2000 | 10886606 |
| virulence factors in vibrios and aeromonads isolated from seafood. | thirty-one isolates from seafood, identified as aeromonas hydrophila (7), aeromonas caviae (11), vibrio parahaemolyticus (3), vibrio fluvialis (5), vibrio alginolytictus (3), vibrio metschnikovii (1) and vibrio damsela (1), were tested for possible virulence factors including extracellular hydrolytic enzymes, haemolysins, cytotoxins (vero and hep-2 cells) and adherence ability (hep-2 cells). all the a. hydrophila strains were beta-haemolytic and produced cytotoxins as well as one strain of v. fl ... | 2001 | 11497085 |
| amplified fragment length polymorphism (aflp) and biochemical typing of photobacterium damselae subsp. damselae. | the aim of the present study was to characterize subspecifically photobacterium damselae subsp. damselae strains isolated from cultured sparus aurata and dicentrarchus labrax by means of phenotypic and molecular typing techniques (amplified fragment length polymorphism, aflp). | 2002 | 12234352 |
| rapidly advancing necrotizing fasciitis caused by photobacterium (vibrio) damsela: a hyperaggressive variant. | to describe the first case of vibrio damsela necrotizing fasciitis in new england, emphasizing the importance of very early operative intervention to achieve source control in this extremely aggressive infection. | 2004 | 14707592 |
| bacterial vaccines for fish--an update of the current situation worldwide. | during the last few years, the use of vaccines for disease prevention in aquaculture has expanded both with regard to the number of fish species and number of microbial diseases. according to the responses to a questionnaire received from 41 countries, vaccination is used in the commercial aquaculture of species like atlantic salmon (salmo salar), rainbow trout (oncorhynchus mykiss), sea bass (dicentrarchus labrax), sea bream (sparus aurata), barramundi (lates calcarifer), tilapia (tilapia spp), ... | 2005 | 15962470 |
| aerobic microorganisms associated with free-ranging bottlenose dolphins in coastal gulf of mexico and atlantic ocean waters. | our abilities to assess health risks to free-ranging dolphin populations, to treat live-stranded or captive dolphins, and to evaluate the risks of disease transmission between humans and dolphins have suffered from a lack of basic information on microorganisms associated with normal, presumably healthy free-ranging individuals. in order to provide these data, we sampled free-ranging bottlenose dolphins (tursiops truncatus) off florida, texas, and north carolina during 1990-2002. blowhole and ana ... | 2006 | 17092884 |
| vibrios of the spotted rose snapper lutjanus guttatus steindachner, 1869 from northwestern mexico. | to characterize and identify vibrios present in wild and cultured juvenile snappers (lutjanus guttatus) in northwestern mexico. | 2007 | 17578416 |
| genes encoding the vibrio harveyi haemolysin (vhh)/thermolabile haemolysin (tlh) are widespread in vibrios. | v. harveyi vhh haemolysin, which shows high homology to the tlh haemolysin (the identities of their deduced amino acid sequences are up to 85.6%), is a putative virulence factor to marine cultured fish. a vhh probe, which is specific to v. harveyi vhha haemolysin gene, was used to screen ecor i digests of total dna from 57 vibrio strains, including 26 vibrio type strains, 20 v. harveyi isolates and 11 v. parahaemolyticus isolates. as a result, 1 strong hybridisation band was detected in 13 type ... | 2007 | 18062266 |
| shewanella and photobacterium spp. in oysters and seawater from the delaware bay. | shewanella algae, s. putrefaciens, and photobacterium damselae subsp. damselae are indigenous marine bacteria and human pathogens causing cellulitis, necrotizing fasciitis, abscesses, septicemia, and death. infections are rare and are most often associated with the immunocompromised host. a study was performed on the microbiological flora of oysters and seawater from commercial oyster harvesting sites in the delaware bay, new jersey. from 276 water and shellfish samples tested, 1,421 bacterial i ... | 2008 | 18378645 |
| photobacterium ganghwense sp. nov., a halophilic bacterium isolated from sea water. | a marine bacterial strain, designated fr1311t, was isolated from a sea-water sample from ganghwa island, south korea. cells were gram-negative, facultatively anaerobic, catalase- and oxidase-positive, motile, oval or rod-shaped and halophilic (optimum sea-salt concentration for growth of 5-6 %). phylogenetic analysis of its 16s rrna gene sequence revealed that it represented a distinct line of descent within the genus photobacterium. the major fatty acids were straight-chain saturated (c(16 : 0) ... | 2006 | 16585687 |
| effect of salinity on the immune response of tiger shrimp penaeus monodon and its susceptibility to photobacterium damselae subsp. damselae. | addition of nacl at 2.5% to tryptic soy broth (tsb) significantly increased the growth of photobacterium damselae subsp. damselae. tiger shrimp penaeus monodon held in 25 per thousand seawater were injected with p. damsela subsp. damselae grown in tsb containing nacl at 0.5%, 1.5%, 2.5% and 3.5% at a dose of 8.48 x 10(4)colony-forming units (cfu)shrimp(-1). over 24-96 h, the cumulative mortality was significantly higher for the shrimp challenged with p. damselae subsp. damselae grown in 2.5% nac ... | 2006 | 16213751 |
| induction of the histidine decarboxylase genes of photobacterium damselae subsp. damselae (formally p. histaminum) at low ph. | to elucidate the detailed mechanism of histamine production by photobacterium damselae subsp. damselae. | 2009 | 19302297 |
| vibrios as causal agents of zoonoses. | vibrios are gram-negative rod-shaped bacteria that are widespread in the coastal and estuarine environments. some species, e.g. vibrio anguillarum and v. tapetis, comprise serious pathogens of aquatic vertebrates or invertebrates. other groups, including grimontia (=vibrio) hollisae, photobacterium (=vibrio) damselae subsp. damselae, v. alginolyticus, v. harveyi (=v. carchariae), v. cholerae, v. fluvialis, v. furnissii, v. metschnikovii, v. mimicus, v. parahaemolyticus and v. vulnificus, may cau ... | 2010 | 19342185 |
| characterization of the 23s and 5s rrna genes and 23s-5s intergenic spacer region (its-2) of photobacterium damselae. | the 23s ribosomal rna (rrna) gene has been sequenced in strains of the fish pathogens photobacterium damselae subsp. damselae (atcc 33539) and subsp. piscicida (atcc 29690), showing that 3 nucleotide positions are clearly different between subspecies. in addition, the 5s rrna gene plus the intergenic spacer region between the 23s and 5s rrna genes (its-2) were amplified, cloned and sequenced for the 2 reference strains as well as the field isolates rg91 (subsp. damselae) and di21 (subsp. piscici ... | 2004 | 15584408 |
| simultaneous detection of marine fish pathogens by using multiplex pcr and a dna microarray. | we coupled multiplex pcr and a dna microarray to construct an assay suitable for the simultaneous detection of five important marine fish pathogens (vibrio vulnificus, listonella anguillarum, photobacterium damselae subsp. damselae, aeromonas salmonicida subsp. salmonicida, and vibrio parahaemolyticus). the array was composed of nine short oligonucleotide probes (25-mer) complementary to seven chromosomal loci (cyt, rpon, gyrb, toxr, urec, dly, and vapa) and two plasmid-borne loci (fata and a.sa ... | 2004 | 15070982 |
| toxicity of photobacterium damselae subsp. damselae strains isolated from new cultured marine fish. | the in vivo and in vitro toxicity of bacterial cells and their extracellular products (ecps) from 16 strains of photobacterium damselae subsp. damselae isolated from 7 epizootic outbreaks were evaluated. on the basis of their 50% lethal dose (ld50) values (about 1 x 10(50 cfu), these strains may be considered as moderately virulent. however, their ecps were strongly lethal for redbanded seabream pagrus auriga causing fish death within 2 h post-inoculation (protein concentration ranged between 2. ... | 2010 | 21166312 |
| accuracy of six commercially available systems for identification of members of the family vibrionaceae. | six commercially available bacterial identification products were tested with vibrio alginolyticus (12 strains), v. cholerae (30 strains), photobacterium (vibrio) damselae (10 strains), v. fluvialis (10 strains), v. furnissii (4 strains), v. hollisae (10 strains), v. metschnikovii (9 strains), v. mimicus (10 strains), v. parahaemolyticus (30 strains), and v. vulnificus (10 strains) to determine the accuracy of each system for identification. the products included api 20e, crystal e/nf, microscan ... | 2003 | 14662957 |
| occurrence of both subspecies of photobacterium damselae in mullets collected in the river magra (italy). | the natural reservoirs and biological characteristics of pathogenic populations of both subspecies of photobacterium damselae in aquatic habitants remain unclear because of difficulties in obtaining pathogenic strains from the environment. in the present study, we assessed the occurrence of photobacterium damselae subsp. piscicida and photobacterium damselae subsp. damselae , considered to be the causative agent of past epizootic outbreaks in mullets collected in the river magra, italy. two hund ... | 2011 | 21529120 |
| The Photobacterium damselae subsp. damselae hemolysins damselysin and HlyA are encoded within a new virulence plasmid. | Photobacterium damselae subsp. damselae (formerly Vibrio damsela) is a marine bacterium that causes infections and fatal disease in a wide range of marine animals and in humans. Highly hemolytic strains produce damselysin (Dly), a cytolysin encoded by the dly gene that is lethal for mice and has hemolytic activity. We found that Dly is encoded in the highly hemolytic strain RM-71 within a 153,429-bp conjugative plasmid that we dubbed pPHDD1. In addition to Dly, pPHDD1 also encodes a homologue of ... | 2011 | 21875966 |
| numerical taxonomy of vibrionaceae isolated from cultured amberjack (seriola dumerili) and surrounding water. | a numerical taxonomic study was performed on 148 isolates of gram-negative, heterotrophic, facultative anaerobic bacteria isolated from amberjack (seriola dumerili) and its surrounding culture water. the study included 30 type and reference strains belonging to genera vibrio, listonella, and photobacterium. the strains were characterized by 109 morphological, biochemical, physiological, and nutritional tests. cluster analysis of similarity matrices obtained with s(sm) and s(j) coefficients was c ... | 2003 | 12567241 |
| high affinity iron-uptake systems in vibrio damsela: role in the acquisition of iron from transferrin. | in this work, the high affinity iron-acquisition systems displayed by virulent and avirulent strains of vibrio damsela have been investigated. this species is an autochthonous member of marine ecosystems that can behave as an opportunistic pathogen for fish and mammals. all strains tested (i) were able to grow under the restricted conditions imposed by the iron chelators transferrin (tf) and eddha, (ii) secreted siderophores of hydroxamic type, other than aerobactin and desferal, that were able ... | 1997 | 12452589 |
| topically acquired bacterial zoonoses from fish: a review. | the main pathogens acquired topically from fish (through spine puncture or open wounds) are aeromonas hydrophila, edwardsiella tarda, erysipelothrix rhusiopathiae, mycobacterium marinum, streptococcus iniae, vibrio vulnificus and vibrio damsela. s. iniae has recently emerged as a public health hazard associated with aquaculture, and m. marinum often infects home aquarium hobbyists. with the expansion of aquaculture and popularity of recreational fishing in australia, medical practitioners can ex ... | 2000 | 11130351 |
| application of aflp for taxonomic and epidemiological studies of photobacterium damselae subsp. piscicida. | a collection of 106 photobacterium damselae subsp. piscicida strains and 19 photobacterium damselae subsp. damselae strains, including reference and type strains, were genetically characterized using aflp. the total genomic dna of each bacterial strain was digested using restriction endonucleases hindiii and taqi. using numerical analysis, six clusters were recognized. the largest cluster (n = 106) contained the majority of the strains tested and consisted exclusively of photobacterium damselae ... | 2000 | 10843040 |
| evidence that water transmits the disease caused by the fish pathogen photobacterium damselae subsp. damselae. | the transmission through water of the disease caused by the fish pathogen, photobacterium damselae subsp. damselae, as well as the role of the skin mucus in the initial steps of the infection, have been studied. all tested strains resisted the bactericidal activity of the mucus and showed an ability to adhere to it, but only those virulent by the intraperitoneal route were infective through water. moribund fishes showed the typical signs of the disease: haemorrhaged areas on the body surface and ... | 2000 | 10747234 |
| necrotizing fasciitis caused by vibrio damsela. | | 1999 | 10220060 |
| novel conjugative transferable multiple drug resistance plasmid paqu1 from photobacterium damselae subsp. damselae isolated from marine aquaculture environment. | the emergence of drug-resistant bacteria is a severe problem in aquaculture. the ability of drug resistance genes to transfer from a bacterial cell to another is thought to be responsible for the wide dissemination of these genes in the aquaculture environment; however, little is known about the gene transfer mechanisms in marine bacteria. in this study, we show that a tetracycline-resistant strain of photobacterium damselae subsp. damselae, isolated from seawater at a coastal aquaculture site i ... | 2012 | 22446310 |
| internalization and cytotoxicity are important virulence mechanisms in vibrio-fish epithelial cell interactions. | vibrio anguillarum and vibrio damselae are gram-negative bacteria that cause systemic infections called vibriosis in fish. they can enter fish cells and survive as intracellular parasites. the host-pathogen interactions between these vibrio species and the fish epithelial cell lines epithelioma papillosum of carp (epc) and grunt-fin tissue (gf) cells, were examined using phase-contrast, scanning electron and confocal microscopy. in addition, potential signal transduction pathways that precede ba ... | 1998 | 9846734 |
| survival of fish-virulent strains of photobacterium damselae subsp. damselae in seawater under starvation conditions. | the survival of fish-virulent strains of photobacterium damselae subsp. damselae in seawater microcosms, with and without sediment, was investigated. the strains survived as culturable bacteria at 14 and 22 degrees c for at least 1 year, and infectivity for fish was maintained. at 5 degrees c, cells lost culturability on solid media, but this was recovered when the temperature was increased to 22 degrees c. finally, morphological changes in the bacterium (rod to coccus), and production of vesicl ... | 1998 | 9835027 |
| classification of fish-pathogenic vibrios based on comparative 16s rrna analysis. | no systematic classification of fish-pathogenic vibrios has been accomplished previously despite the use of serological, physiological, and genetical classification systems. in this study, a comparative 16s rrna analysis of 34 strains (representing seven species) of fish-pathogenic vibrios was performed. the 16s rrna sequences were obtained by using reverse transcriptase. nearly complete sequences were obtained for nine strains. on the basis of the results of this analysis, the remaining strains ... | 1995 | 8590667 |
| fatal necrotizing fasciitis due to vibrio damsela. | a patient who succumbed to fulminant necrotizing fasciitis due to vibrio damsela after injury by a rabbitfish is described. despite the absence of any known underlying illness, he did not respond to appropriate antibiotic therapy and radical surgical intervention. this represents the first documented case of necrotizing fasciitis due to this organism, and is also the first reported case in southeast asia inflicted by rabbitfish. | 1993 | 8284652 |
| isolation and characterization of two hemolytic phenotypes of vibrio damsela associated with a fatal wound infection. | two hemolytic phenotypes of vibrio damsela, isolated from the tissue of a patient with a fatal wound infection, were characterized. the patient had underlying disease, and the wound was associated with an injury inflicted during the handling of a catfish. the phenotypes were morphologically and biochemically similar except for their lecithinase, lipase, and hemolytic activities. when grown on rabbit blood agar, one phenotype (lz) produced a large zone of hemolysis (10 mm) around the colony, wher ... | 1985 | 3980686 |
| vibrio damsela infection in a stranded leatherback turtle (dermochelys coriacea). | necropsy of a stranded adult leatherback turtle (dermochelys coriacea) determined that the animal died as a result of valvular endocarditis and septicemia. vibrio damsela was isolated from the endocardial thrombus. the route of entry for infection probably was through the gastrointestinal tract. | 1987 | 3682094 |
| the degradation of platelet-activating factor and related lipids: susceptibility to phospholipases c and d. | 1-o-octadecyl-2-o-methyl-rac-glycero-3-phosphocholine (et-18-och3) is an ether-linked lipid that exhibits selective cytotoxicity toward several types of tumor cells and is relatively inactive toward normal cells under the same conditions of treatment. the mechanis of this selective cytotoxicity is unknown. we conducted studies to determine whether this compound is metabolized by phospholipases c and d and, if so, whether sensitive and resistant cells differ in their ability to degrade et-18-och3 ... | 1987 | 3444369 |
| cytolytic toxins of vibrio vulnificus and vibrio damsela. | | 1988 | 3231100 |
| aeromonas schubertii, a new mannitol-negative species found in human clinical specimens. | in 1983 the vernacular name enteric group 501 was coined for a group of strains that had been referred to our laboratory as "possible vibrio damsela that does not require nacl for growth." by dna-dna hybridization (hydroxyapatite method, 32p, 60 and 75 degrees c), six strains of enteric group 501 were closely related to the labeled strain 2446-81 (70 to 95% at 60 degrees c and 71 to 93% at 75 degrees c; 0 to 1% divergence). type strains of all aeromonas species and reference strains of six other ... | 1988 | 3139706 |
| vibrio damsela wound infections in australia. | | 1989 | 2811729 |
| deaths in captive eels (anguila reinhardtii) due to photobacterium (vibrio) damsela. | | 1992 | 1530562 |
| difference of genotypic and phenotypic characteristics and pathogenicity potential of photobacterium damselae subsp. damselae between clinical and environmental isolates from japan. | photobacterium damselae subsp. damselae has been known as an opportunistic pathogen in fish and mammals. human infectious cases are often very serious and occasionally fatal. we previously reportedtwo fatal cases caused by this subspecies where the patients developed multiple organ failure within 20-36 h after the onset of initial symptoms. despite its ability to cause serious infections in humans, this subspecies has not been well studied because human infectious cases caused by this subspecies ... | 2008 | 18554860 |
| co-occurrence of viral and bacterial pathogens in disease outbreaks affecting newly cultured sparid fish. | several microbial disease outbreaks in farm stocks of newly cultured sparid fish species, such as common seabream, redbanded seabream, and white seabream, were recorded from 2004 to 2006. this study describes the isolation and characterization of the potential causative agents, either bacteria or viruses, of these outbreaks. the isolated bacterial strains were characterized according to traditional taxonomical analyses and sequencing of a 16s rdna fragment. most bacteria were identified as vibri ... | 2007 | 18076001 |
| vibrio damsela, a marine bacterium, causes skin ulcers on the damselfish chromis punctipinnis. | a previously undescribed marine bacterium, vibrio damsela, was isolated from naturally occurring skin ulcers on a species of temperate-water damselfish, the blacksmith (chromis punctipinnis). laboratory infection of the blacksmith with vibrio damsela produced similar ulcers. vibrio damsela was pathogenic for four other species of damselfish but not for members of other families of fish. the bacterium has also been isolated from water and from two human wounds and may be a cause of human disease. | 1981 | 17755898 |
| surveillance of health status on eight marine rainbow trout, oncorhynchus mykiss (walbaum), farms in denmark in 2006. | the health status of eight marine rainbow trout farms was followed from mid-june to mid-september 2006 by sampling both dead and healthy fish approximately every 2 weeks for bacteriological and virological investigation. no fish pathogenic viruses were detected, but all farms experienced disease and mortality as a result of various bacterial infections. yersinia ruckeri was found on four and renibacterium salmoninarum on five of the farms, but only during the first part of the surveillance perio ... | 2008 | 18786028 |
| photobacterium damselae subsp. damselae, an emerging pathogen in danish rainbow trout, oncorhynchus mykiss (walbaum), mariculture. | a selection of 16 field isolates of photobacterium damselae from marine rainbow trout farms in denmark was subjected to phenotypic and genotypic characterization and pathogenicity to fish. all isolates belonged to the subspecies damselae, being positive for haemolysis, motility and urease. there were considerable differences in haemolytic properties, some isolates presenting a broad zone of haemolysis and others only a narrow zone. pulsed-field gel electrophoresis revealed a high diversity indic ... | 2009 | 19364386 |
| synergistic and additive effects of chromosomal and plasmid-encoded hemolysins contribute to hemolysis and virulence in photobacterium damselae subsp. damselae. | photobacterium damselae subsp. damselae causes infections and fatal disease in marine animals and in humans. highly hemolytic strains produce damselysin (dly) and plasmid-encoded hlya (hlya(pl)). these hemolysins are encoded by plasmid pphdd1 and contribute to hemolysis and virulence for fish and mice. in this study, we report that all the hemolytic strains produce a hitherto uncharacterized chromosome-encoded hlya (hlyach). hemolysis was completely abolished in a single hlyach mutant of a plasm ... | 2013 | 23798530 |
| culturable microbiota of ranched southern bluefin tuna (thunnus maccoyii castelnau). | the australian tuna industry is based on the ranching of wild southern bluefin tuna (sbt, thunnus maccoyii). within this industry, only opportunistic pathogens have been reported infecting external wounds of fish. this study aimed to identify different culturable bacteria present in three cohorts of sbt and to determine normal bacteria and potential pathogens in isolates from harvest fish and moribund/dead fish. post-mortem changes in the microbiota were also studied. | 2013 | 23789748 |
| prevalence of listeria, aeromonas, and vibrio species in fish used for human consumption in turkey. | a total of 78 raw retail fish samples from 30 freshwater and 48 marine fish were examined for the presence of listeria, aeromonas, and vibrio species. the overall incidence of listeria spp. was 30% in freshwater samples and 10.4% in marine fish samples. listeria monocytogenes (44.5%) was the most commonly isolated species in freshwater fish, and listeria murrayi (83.5%) was the most commonly isolated species in marine fish samples. motile aeromonads were more common in marine fish samples (93.7% ... | 2010 | 20132688 |
| pulmonary fungal infection caused by neoscytalidium dimidiatum in a risso's dolphin (grampus griseus). | neoscytalidium dimidiatum was isolated from two 12-18 cm abscesses in the lung and the mediastinal lymph nodes of a stranded risso's dolphin (grampus griseus). histopathologic examination of samples of these organs revealed the presence of hyphae and sclerotic body-like fungal elements. photobacterium damselae subsp. damselae was recovered from the dolphin's organs which also were found to contain numerous monorygma grimaldii cysts. no histopathological signs of morbillivirus infection were seen ... | 2010 | 21174494 |
| In vitro evaluation of antibacterial activity of Asparagopsis taxiformis from the Straits of Messina against pathogens relevant in aquaculture. | Ethanol extracts of Asparagopsis taxiformis collected from the Straits of Messina (Italy) were screened for antibacterial activity against pathogenic shellfish and fish bacteria previously isolated from local marine and brackish environments. Genetic labelling by DNA barcoding allowed us to identify the algal population as a biogeographical strain conspecific to A. taxiformis. The extract obtained in May showed the broadest antibacterial activity against all tested pathogenic bacteria, especiall ... | 2012 | 22078751 |
| severe wound infection with photobacterium damselae ssp. damselae and vibrio harveyi, following a laceration injury in marine environment: a case report and review of the literature. | marine microorganisms are uncommon etiologies of skin and skin structure infections, that is, wound infections. we report a case of severe wound infection, caused by the marine photobacterium damselae (vibrionaceae), in a 64-year-old male patient, returning from australia. the isolate tested positive for pphdd1, a plasmid conferring high-level virulence. furthermore, the wound was coinfected with vibrio harveyi, a halophile bacterium, which has never been reported from human infections before. i ... | 2013 | 24171004 |
| antimicrobial and herbal drug resistance in enteric bacteria isolated from faecal droppings of common house lizard/gecko (hemidactylus frenatus). | from 194 faecal dropping samples of common house geckos collected from offices (60), houses (88), integrated farm units (ifs,18) and hostels, guest houses, and dining rooms of different canteen/mess (hgm, 28), 326 bacterial isolates of enteric bacteria belonging to 17 genera and 34 species were detected. escherichia coli were the most frequently (39) isolated followed by citrobacter freundii (33), klebsiella pneumonia (27), salmonella indica (12), enterobacter gergoviae (12), and ent. agglomeran ... | 2013 | 24223595 |
| preliminary study on the in vitro and in vivo effects of asparagopsis taxiformis bioactive phycoderivates on teleosts. | several compounds from marine organisms have been studied for their potential use in aquaculture. among the red algae, asparagopsis taxiformis is considered one of the most promising species for the production of bioactive metabolites with numerous proposed applications. here, the in vitro antibacterial activity, the easy handling and the absence of adverse effects on marine fish species are reported. depending on the seasonal period of sampling, ethanol extracts of a. taxiformis exhibited signi ... | 2016 | 27826246 |
| novel macrolide-resistance genes, mef(c) and mph(g), carried by plasmids from vibrio and photobacterium isolated from sediment and seawater of a coastal aquaculture site. | the aim of this study was to determine whether mef(c) and mph(g), originally found on the transferable multi-drug plasmid paqu1 from photobacterium damselae subsp. damselae isolated from seawater of a fish farm, are responsible for conferring macrolide resistance. since these genes are localized head-to-tail on paqu1 and only four nucleotides exist between them, the single- and combination-effect of these genes was examined. when mph(g) alone was introduced to escherichia coli, the minimum inhib ... | 2015 | 25765542 |
| characterization of a new beta-lactamase gene from isolates of vibrio spp. in korea. | pcr was performed to analyze the beta-lactamase genes carried by ampicillin-resistant vibrio spp. strains isolated from marine environments in korea between 2006 and 2009. all 36 strains tested showed negative results in pcr with the primers designed from the nucleotide sequences of various known beta-lactamase genes. this prompted us to screen new beta-lactamase genes. a novel beta-lactamase gene was cloned from vibrio alginolyticus kv3 isolated from the aquaculture water of geoje island of kor ... | 2012 | 22534305 |
| prevalence and characterization of high histamine-producing bacteria in gulf of mexico fish species. | recent developments in detection and enumeration of histamine-producing bacteria (hpb) have created powerful molecular-based tools to better understand the presence of spoilage bacteria and conditions, resulting in increased risk of scombrotoxin fish poisoning. we examined 235 scombrotoxin-forming fish from the gulf of mexico for the presence of high hpb. photobacterium damselae subsp. damselae was the most prevalent hpb (49%), followed by morganella morganii (14%), enterobacter aerogenes (4%), ... | 2015 | 26197285 |
| chromosome-encoded hemolysin, phospholipase and collagenase contribute to virulence for fish in plasmidless isolates of photobacterium damselae subsp. damselae. | photobacterium damselae subsp. damselae is a pathogen of marine animals including fish of importance in aquaculture. the virulence plasmid pphdd1, characteristic of highly hemolytic isolates, encodes the hemolysins damselysin (dly) and phobalysin (phlyp). strains lacking pphdd1 constitute the vast majority of the isolates from fish outbreaks, but genetic studies to identify virulence factors in plasmidless strains are scarce. here we show that the chromosome-i encoded hemolysin phlyc plays a rol ... | 2017 | 28341681 |
| a proteomic analysis of the iron response of photobacterium damselae subsp. damselae reveals metabolic adaptations to iron levels changes and novel potential virulence factors. | photobacterium damselae subsp. damselae (pdd) is a marine bacterium that can infect numerous species of marine fish as well as other species including humans. low iron availability is one of the signs that bacterial pathogens can detect in order to begin colonizing their host, and the reduction of iron levels is a nonspecific host defense strategy that prevents bacterial proliferation. in this work a proteomic approach was used to study the gene expression adaptations of a pdd strain in response ... | 2017 | 28284618 |
| repair of a bacterial small β-barrel toxin pore depends on channel width. | membrane repair emerges as an innate defense protecting target cells against bacterial pore-forming toxins. here, we report the first paradigm of ca(2+)-dependent repair following attack by a small β-pore-forming toxin, namely, plasmid-encoded phobalysin of photobacterium damselae subsp. damselae in striking contrast, vibrio cholerae cytolysin, the closest ortholog of phobalysin, subverted repair. mutational analysis uncovered a role of channel width in toxicity and repair. thus, the replacement ... | 2017 | 28196960 |
| a new multilocus sequence typing scheme and its application for the characterization of photobacterium damselae subsp. damselae associated with mortality in cetaceans. | photobacterium damselae subsp. damselae (pdd) is a known pathogen of fish, humans and marine mammals. in this study, a multilocus sequence typing (mlst) scheme based on six housekeeping genes (glp, gyrb, metg, pnt, pyrc, and toxr) was developed to better understand the pdd population structure and used to type 73 pdd isolates from cetaceans, mainly striped dolphins (stenella coeruleoalba) involved in mortality episodes, and from a few marine chelonians. five reference atcc strains were also incl ... | 2016 | 27818651 |
| first report of isolation and characterization of photobacterium damselae subsp. damselae from cage-farmed cobia (rachycentron canadum). | | 2016 | 27696450 |
| photobacterium damselae subsp. damselae, an emerging fish pathogen in the black sea: evidence of a multiclonal origin. | photobacterium damselae subsp. damselae is considered to be an emerging pathogen of marine fish of importance in aquaculture, with a notable increase in its geographical distribution during the last several years. in this study, we carried out for the first time to our knowledge a genetic and pathobiological characterization of 14 strains isolated from sea bass (dicentrarchus labrax) reared in the southeastern black sea, where high mortalities were observed at two aquaculture farms during the su ... | 2016 | 27084008 |
| photobacterium damselae subsp. damselae major virulence factors dly, plasmid-encoded hlya, and chromosome-encoded hlya are secreted via the type ii secretion system. | photobacterium damselae subsp. damselae is a marine bacterium that causes septicemia in marine animals and in humans. previously, we had determined a major role of pphdd1 plasmid-encoded dly (damselysin) and hlya (hlyapl) and the chromosome-encoded hlya (hlyach) hemolysins in virulence. however, the mechanisms by which these toxins are secreted remain unknown. in this study, we found that a mini-tn10 transposon mutant in a plasmidless strain showing an impaired hemolytic phenotype contained an i ... | 2015 | 25583529 |
| evidence for horizontal gene transfer, gene duplication and genetic variation as driving forces of the diversity of haemolytic phenotypes in photobacterium damselae subsp. damselae. | photobacterium damselae subsp. damselae, a marine bacterium that causes infections in marine animals and in humans, produces up to three different haemolysins involved in virulence, which include the pphdd1 plasmid-encoded damselysin (dly) and hlyapl , and the chromosome-encoded hlyach . we screened 45 isolates from different origins, and found a correlation between their haemolytic phenotypes and the differential haemolysin gene content. all highly and medium haemolytic strains harboured pphdd1 ... | 2014 | 24813762 |
| virulence properties, biofilm formation and random amplified polymorphic dna analysis of photobacterium damselae subsp. damselae isolates from cultured sea bream (sparus aurata) and sea bass (dicentrarchus labrax). | photobacterium damselae subsp. damselae has been isolated from different outbreaks affecting cultured sparus aurata and dicentrarchus labrax. the aim of the present study was to characterize the phenotype and genotype of 12 p. damselae subsp. damselae strains isolated from these outbreaks. the roles of skin mucus in resistance to the initial infection steps have been studied. all tested strains resisted the bactericidal activity of the mucus and showed an ability to adhere to it, but only those ... | 2014 | 24685699 |
| photobacterium damselae subsp. damselae, a bacterium pathogenic for marine animals and humans. | photobacterium damselae subsp. damselae (formerly vibrio damsela) is a pathogen of a variety of marine animals including fish, crustaceans, molluscs, and cetaceans. in humans, it can cause opportunistic infections that may evolve into necrotizing fasciitis with fatal outcome. although the genetic basis of virulence in this bacterium is not completely elucidated, recent findings demonstrate that the phospholipase-d dly (damselysin) and the pore-forming toxins hlyapl and hlyach play a main role in ... | 2013 | 24093021 |
| antibiotic resistance and molecular typing of photobacterium damselae subsp. damselae, isolated from seafood. | the objectives of our study is to determinate the antibiotic susceptibility of this organism to different antibiotics to determine the discriminatory power of the molecular typing methods. | 2013 | 23230901 |
| metagenomic assessment of the eastern oyster-associated microbiota. | bacteria associated with the eastern oysters (crassostrea virginica) native to apalachicola bay, fl, were investigated using 16s rrna gene amplicon metagenomic sequencing which revealed that the oyster microbiome was predominated by cyanobacteria and proteobacteria. we also found that the oyster tissues were predominated by the pathogenic and symbiotic photobacterium spp. (formerly known as vibrio damselae). | 2014 | 25342691 |
| rstb regulates expression of the photobacterium damselae subsp. damselae major virulence factors damselysin, phobalysin p and phobalysin c. | the marine pathogenic bacterium photobacterium damselae subsp. damselae causes septicemia in marine animals and in humans. the pphdd1 plasmid-encoded hemolysins damselysin (dly) and phobalysin p (phlyp), and the chromosome-encoded hemolysin phobalysin c (phlyc) constitute its main virulence factors. however, the mechanisms by which expression of these three hemolysins is regulated remain unknown. here we report the isolation of a mini-tn10 transposon mutant which showed a strong impairment in it ... | 2017 | 28443076 |
| development of a real-time pcr assay for rapid detection and quantification of photobacterium damselae subsp. piscicida in fish tissues. | the availability of a rapid and accurate method for the diagnosis of photobacterium damselae subsp. piscicida (phdp), able to discriminate its strictly correlated subsp. damselae (phdd), formally known as vibrio damsela, is essential for managing fish pasteurellosis outbreaks in farmed fish. a single-step, high-sensitivity real-time pcr assay for simultaneous detection and quantification of p. damselae was designed targeting partial of the sequence of the bamb gene and tested for specificity and ... | 2017 | 28857188 |
| secreted citrate serves as iron carrier for the marine pathogen photobacterium damselae subsp damselae. | photobacterium damselae subsp damselae (pdd) is a vibrionaceae that has a wide pathogenic potential against many marine animals and also against humans. some strains of this bacterium acquire iron through the siderophore vibrioferrin. however, there are virulent strains that do not produce vibrioferrin, but they still give a strong positive reaction in the cas test for siderophore production. in an in silico search on the genome sequences of this type of strains we could not find any orf which c ... | 2017 | 28848719 |
| detection and characterization of histamine-producing strains of photobacterium damselae subsp. damselae isolated from mullets. | photobacterium damselae subsp. damselae (pdd) is considered to be an emerging pathogen of marine fish and has also been implicated in cases of histamine food poisoning. in this study, eight strains isolated from mullets of the genera mugil and liza captured in the ligurian sea were characterized, and a method to detect histamine-producing pdd from fish samples was developed. the histamine-producing potential of the strains was evaluated in culture media (tsb+) using a histamine biosensor. subseq ... | 2017 | 29056690 |
| revisiting the genus photobacterium: taxonomy, ecology and pathogenesis. | the genus photobacterium, one of the eight genera included in the family vibrionaceae, contains 27 species with valid names and it has received attention because of the bioluminescence and pathogenesis mechanisms that some of its species exhibit. however, the taxonomy and phylogeny of this genus are not completely elucidated; for example, p. logei and p. fischeri are now considered members of the genus aliivibrio, and previously were included in the genus vibrio. in addition, p. damselae subsp. ... | 2017 | 28581017 |
| sphingomyelinase d/ceramide 1-phosphate in cell survival and inflammation. | sphingolipids are major constituents of biological membranes of eukaryotic cells. many studies have shown that sphingomyelin (sm) is a major phospholipid in cell bilayers and is mainly localized to the plasma membrane of cells, where it serves both as a building block for cell architecture and as a precursor of bioactive sphingolipids. in particular, upregulation of (c-type) sphingomyelinases will produce ceramide, which regulates many physiological functions including apoptosis, senescence, or ... | 2015 | 25938271 |
| first time isolation of photobacterium damselae subsp. damselae from caranx sexfasciatus in persian gulf, iran. | photobacterium damselae subsp. damselae is a marine pathogenic bacterium which causes disease in marine animals and human. this bacterium mostly found in coastal shallow seawater. so, the aim of this study was isolation and characterization of photobacterium damselae subsp. damselae from edible fish of persian gulf, bandar abbas. | 2015 | 26668707 |
| phobalysin, a small β-pore-forming toxin of photobacterium damselae subsp. damselae. | photobacterium damselae subsp. damselae, an important pathogen of marine animals, may also cause septicemia or hyperaggressive necrotizing fasciitis in humans. we previously showed that hemolysin genes are critical for virulence of this organism in mice and fish. in the present study, we characterized the hlya gene product, a putative small β-pore-forming toxin, and termed it phobalysin p (phlyp), for "photobacterial lysin encoded on a plasmid." phlyp formed stable oligomers and small membrane p ... | 2015 | 26303391 |
| vitamin a supplementation enhances senegalese sole (solea senegalensis) early juvenile's immunocompetence: new insights on potential underlying pathways. | senegalese sole (solea senegalensis) has been considered since the 1990's to be a promising flatfish species for diversifying european marine aquaculture. however, pathogen outbreaks leading to high mortality rates can impair senegalese sole commercial production at the weaning phase. different approaches have been shown to improve fish immunocompetence; with this in mind the objective of the work described herein was to determine whether increased levels of dietary vitamin a (va) improve the im ... | 2015 | 26272637 |
| characterization of a novel bacteriophage, phda1, infecting the histamine-producing photobacterium damselae subsp. damselae. | photobacterium damselae subsp. damselae is a potent histamine-producing micro-organism. the aim of this study was to isolate and characterize a bacteriophage phda1 that infected p. damselae subsp. damselae to inhibit its growth and histamine accumulation. | 2015 | 25810004 |
| novel host-specific iron acquisition system in the zoonotic pathogen vibrio vulnificus. | vibrio vulnificus is a marine bacterium associated with human and fish (mainly farmed eels) diseases globally known as vibriosis. the ability to infect and overcome eel innate immunity relies on a virulence plasmid (pvvbt2) specific for biotype 2 (bt2) strains. in the present study, we demonstrated that pvvbt2 encodes a host-specific iron acquisition system that depends on an outer membrane receptor for eel transferrin called vep20. the inactivation of vep20 did not affect either bacterial growt ... | 2015 | 25630302 |