| structural and functional comparisons and production of recombinant crustacean hyperglycemic hormone (chh) and chh-like peptides from the mud crab scylla olivacea. | sco-chh and sco-chh-l (chh-like peptide), two structural variants of the crustacean hyperglycemic hormone family identified in the mud crab (scylla olivacea), are presumably alternatively spliced gene products. in this study, sco-chh and sco-chh-l were isolated from the tissues using high performance liquid chromatography. identity of the native peptides was confirmed using mass spectrometric (ms) analyses of purified materials and of trypsin-digested peptide fragments. additionally, characteriz ... | 2010 | 20171218 |
| mud crab susceptibility to disease from white spot syndrome virus is species-dependent. | abstract: | 2010 | 21092125 |
| identification of genes associated with reproduction in the mud crab (scylla olivacea) and their differential expression following serotonin stimulation. | the central nervous system (cns) is often intimately involved in reproduction control and is therefore a target organ for transcriptomic investigations to identify reproduction-associated genes. in this study, 454 transcriptome sequencing was performed on pooled brain and ventral nerve cord of the female mud crab (scylla olivacea) following serotonin injection (5 µg/g bw). a total of 197,468 sequence reads was obtained with an average length of 828 bp. approximately 38.7% of 2,183 isotigs matche ... | 2014 | 25542017 |
| functional assessment of residues in the amino- and carboxyl-termini of crustacean hyperglycemic hormone (chh) in the mud crab scylla olivacea using point-mutated peptides. | to assess functional importance of the residues in the amino- and carboxyl-termini of crustacean hyperglycemic hormone in the mud crab scylla olivacea (sco-chh), both wild-type and point-mutated chh peptides were produced with an amidated c-terminal end. spectral analyses of circular dichroism, chromatographic retention time, and mass spectrometric analysis of the recombinant peptides indicate that they were close in conformation to native chh and were produced with the intended substitutions. t ... | 2015 | 26261986 |
| transcriptome analysis and differential gene expression on the testis of orange mud crab, scylla olivacea, during sexual maturation. | adequate genetic information is essential for sustainable crustacean fisheries and aquaculture management. the commercially important orange mud crab, scylla olivacea, is prevalent in southeast asia region and is highly sought after. although it is a suitable aquaculture candidate, full domestication of this species is hampered by the lack of knowledge about the sexual maturation process and the molecular mechanisms behind it, especially in males. to date, data on its whole genome is yet to be r ... | 2017 | 28135340 |
| mitochondrial dna diversity of mud crab scylla olivacea (portunidae) in peninsular malaysia: a preliminary assessment. | a primary factor in population management and wildlife conservation is the delineation of population units derived from descriptions of population genetic structure. yet, predicting factors that influence the patterns of gene flow in a population particularly at landscape scales remains a major challenge in evolutionary biology. here we report a population genetic study of the mud crab scylla olivacea examined based on a 542 bp segment of the mitochondrial dna cytochrome c oxidase i gene among 9 ... | 2013 | 24062076 |
| characterization of the circulating hemocytes in mud crab (scylla olivacea) revealed phenoloxidase activity. | this study focused on an isolation and characterization of the circulating hemocytes in mud crab, scylla olivacea. isolation of specific cell types of hemocytes from crab hemolymph was accomplished by using 60% percoll density gradient centrifugation. four separated bands of the hemocytes were successfully obtained. characterization of these isolated hemocytes by light microscope using trypan blue-rose bengal staining, rose bengal-hematoxilin staining, and phase contrast revealed four distinct t ... | 2014 | 24316230 |