| taxonomic relationships of the [pasteurella] haemolytica complex as evaluated by dna-dna hybridizations and 16s rrna sequencing with proposal of mannheimia haemolytica gen. nov., comb. nov., mannheimia granulomatis comb. nov., mannheimia glucosida sp. nov., mannheimia ruminalis sp. nov. and mannheimia varigena sp. nov. | the present paper presents the conclusions of a polyphasic investigation of the taxonomy of the trehalose-negative [pasteurella] haemolytica complex. clusters previously identified by ribotyping and multilocus enzyme electrophoresis (mee) have been evaluated by 16s rrna sequencing and dna-dna hybridizations. results obtained by the different techniques were highly related and indicated that the [p.] haemolytica complex contains distinct genetic and phenotypic groups. at least seven species were ... | 1999 | 10028248 |
| sequence diversity and molecular evolution of the leukotoxin (lkta) gene in bovine and ovine strains of mannheimia (pasteurella) haemolytica. | the molecular evolution of the leukotoxin structural gene (lkta) of mannheimia (pasteurella) haemolytica was investigated by nucleotide sequence comparison of lkta in 31 bovine and ovine strains representing the various evolutionary lineages and serotypes of the species. eight major allelic variants (1.4 to 15.7% nucleotide divergence) were identified; these have mosaic structures of varying degrees of complexity reflecting a history of horizontal gene transfer and extensive intragenic recombina ... | 2001 | 11157953 |
| tetracycline resistance genes in isolates of pasteurella multocida, mannheimia haemolytica, mannheimia glucosida and mannheimia varigena from bovine and swine respiratory disease: intergeneric spread of the tet(h) plasmid pmht1. | tetracycline-resistant isolates of pasteurella multocida and mannheimia spp. from respiratory diseases in cattle and swine were investigated for the classes of tet gene and their chromosomal or plasmid location. the 34 isolates comprised eight p. multocida, 23 mannheimia haemolytica, two mannheimia varigena and a single mannheimia glucosida isolate. identification of the tet genes was achieved by pcr analysis and hybridization with specific probes. transformation and hybridization experiments se ... | 2001 | 11679552 |
| mosaic structure and molecular evolution of the leukotoxin operon (lktcabd) in mannheimia (pasteurella) haemolytica, mannheimia glucosida, and pasteurella trehalosi. | the mosaic structure and molecular evolution of the leukotoxin operon (lktcabd) was investigated by nucleotide sequence comparison of the lktc, lktb, and lktd genes in 23 mannheimia (pasteurella) haemolytica, 6 mannheimia glucosida, and 4 pasteurella trehalosi strains. sequence variation in the lkta gene has been described previously (r. l. davies et al., j. bacteriol. 183:1394-1404, 2001). the leukotoxin operon of m. haemolytica has a complex mosaic structure and has been derived by extensive i ... | 2002 | 11741868 |
| sequence diversity and molecular evolution of the heat-modifiable outer membrane protein gene (ompa) of mannheimia(pasteurella) haemolytica, mannheimia glucosida, and pasteurella trehalosi. | the ompa (or heat-modifiable) protein is a major structural component of the outer membranes of gram-negative bacteria. the protein contains eight membrane-traversing beta-strands and four surface-exposed loops. the genetic diversity and molecular evolution of ompa were investigated in 31 mannheimia (pasteurella) haemolytica, 6 mannheimia glucosida, and 4 pasteurella trehalosi strains by comparative nucleotide sequence analysis. the ompa proteins of m. haemolytica and m. glucosida contain four h ... | 2004 | 15317779 |
| tet(l)-mediated tetracycline resistance in bovine mannheimia and pasteurella isolates. | tetracycline-resistant mannheimia and pasteurella isolates, which were negative for the tetracycline resistance genes (tet) commonly detected among these bacteria, were investigated for other tet genes present and their location. | 2005 | 15972309 |
| complete nucleotide sequence of a p2 family lysogenic bacteriophage, varphimhaa1-phl101, from mannheimia haemolytica serotype a1. | the 34,525 nucleotide sequence of a double-stranded dna bacteriophage (phimhaa1-phl101) from mannheimia haemolytica serotype a1 has been determined. the phage encodes 50 open reading frames. twenty-three of the proteins are similar to proteins of the p2 family of phages. other protein sequences are most similar to possible prophage sequences from the draft genome of histophilus somni 2336. fourteen open reading frames encode proteins with no known homolog. the p2 orthologues are collinear in phi ... | 2006 | 16631219 |
| diversity of temperate bacteriophages induced in bovine and ovine mannheimia haemolytica isolates and identification of a new p2-like phage. | the diversity of temperate bacteriophages was examined in 32 mannheimia haemolytica, six mannheimia glucosida and four pasteurella trehalosi isolates. phage particles were induced and identified by electron microscopy in 24 (75%) m. haemolytica isolates, but in only one (17%) m. glucosida and one (25%) p. trehalosi isolate. the m. haemolytica phages were relatively diverse as seven siphoviridae, 15 myoviridae and two podoviridae-like phages were identified; the myoviridae-type phages also exhibi ... | 2006 | 16842340 |
| characterisation of mannheimia spp. strains isolated from bovine nasal exudate and factors associated to isolates, in dairy farms in the central valley of mexico. | mannheimia spp. strains obtained from bovine nasal exudates of either clinically healthy or clinically affected by respiratory tract disease animals were isolated and characterised to estimate the prevalence of isolated serotypes in dairy farms in mexico, by means of a trans-sectional descriptive study. strains were isolated and typified through biochemical and immunological tests. chi(2) or fisher statistical tests were applied, as well as odds ratio calculation and logistic regression analysis ... | 2008 | 17521689 |
| a multiplex polymerase chain reaction assay for the identification of mannheimia haemolytica, mannheimia glucosida and mannheimia ruminalis. | the objective of this study was to design a multiplex pcr assay to identify mannheimia haemolytica, mannheimia glucosida and mannheimia ruminalis. the multiplex pcr included primer sets hp, amplifying a dna region from an unknown hypothetical protein, lkt and lkt2, amplifying different regions of the leukotoxind gene, and 16s to amplify universal bacterial sequences of the 16s rrna gene. based on positive amplification, isolates were delineated as m. haemolytica (hp, lkt, 16s), m. glucosida (hp, ... | 2008 | 18308486 |
| respiratory disease in calves: microbiological investigations on trans-tracheally aspirated bronchoalveolar fluid and acute phase protein response. | trans-tracheal aspirations from 56 apparently healthy calves and 34 calves with clinical signs of pneumonia were collected in six different herds during september and november 2002. the 90 samples were cultivated and investigated by pcr tests targeting the species histophilus somni, mannheimia haemolytica, pasteurella multocida, mycoplasma bovis, mycoplasma dispar, and mycoplasma bovirhinis. a pcr test amplifying the lktc-artj intergenic region was evaluated and shown to be specific for the two ... | 2009 | 19186010 |
| [fatty acid composition of cells and lipopolysaccliarides of mannheimia haemolytica, mannheimia glucosida and bibersteinia trehalosi strains]. | the mannheimia haemolytica, mannheimia glucosida and bibersteinia trehalosi strains and the similar fatty acid composition of cells with domination of c(16:1) and c(16:0), which were in almost equal quantities, c(14:0 and c(18:1) + c(18:2). the fatty acid composition of lipopolysaccharides (lps) of the studied bacteria had no essential differences too. it was mainly represented by c(14:0) and 3-oh-c(14:0) which consisted of more than 80% of all lps fatty acids. c(12:0), c(16:1) and c(16:0) were ... | 2008 | 19351048 |
| comparison of repetitive pcr and pulsed-field gel electrophoresis for the genotyping of mannheimia haemolytica. | mannheimia haemolytica is an opportunistic pathogen that can cause fibrinonecrotic pneumonia in cattle and is the main bacterial agent implicated in bovine respiratory disease-complex (brd). despite its economic importance to the cattle industry, few studies have characterized the genetic nature of m. haemolytica and none have genotyped isolates from feedlots. identifying and monitoring genetic variants of m. haemolytica is important to understanding the etiology of brd in cattle. we investigate ... | 2010 | 20122972 |
| mannheimia species associated with ovine mastitis. | mannheimia glucosida, m. haemolytica, and m. ruminalis were isolated from cases of acute mastitis in ewes. m. glucosida was found to be a common cause of clinical mastitis in sheep. selected phenotypic tests in addition to genotyping were needed to definitively identify mannheimia species causing ovine mastitis. | 2010 | 20660220 |
| mannheimia caviae sp. nov., isolated from epidemic conjunctivitis and otitis media in guinea pigs. | strains t138021/75t, pg 19 and pg 20 (taxon 25 of bisgaard) all isolated from guinea pigs were characterized. strains t138021-75 t and pg20 showed identical 16s rrna gene sequences and were distantly related with the highest 16s rrna similarity of 98.6 % to the published strain p224, while the type strain of mannheimia glucosida and other strains of mannheimia glucosida demonstrated 97.8% similarity, while the type strain of mannheimia varigena showed 97.3% 16s rrna similarity, whereas all other ... | 2010 | 20729306 |
| evidence for a common gene pool and frequent recombinational exchange of the tbpba operon in mannheimia haemolytica, mannheimia glucosida and bibersteinia trehalosi. | the tbpba operon was sequenced in 42 representative isolates of mannheimia haemolytica (32), mannheimia glucosida (6) and bibersteinia trehalosi (4). a total of 27 tbpb and 20 tbpa alleles were identified whilst the tbpba operon was represented by 28 unique alleles that could be assigned to seven classes. there were 1566 (34.8% variation) polymorphic nucleotide sites and 482 (32.1% variation) variable inferred amino acid positions among the 42 tbpba sequences. the tbpba operons of serotype a2 m. ... | 2010 | 20884693 |
| microbial diversity and dynamics during the production of may bryndza cheese. | diversity and dynamics of microbial cultures were studied during the production of may bryndza cheese, a traditional slovak cheese produced from unpasteurized ewes' milk. quantitative culture-based data were obtained for lactobacilli, lactococci, total mesophilic aerobic counts, coliforms, e. coli, staphylococci, coagulase-positive staphylococci, yeasts, fungi and geotrichum spp. in ewes' milk, curd produced from it and ripened for 0 - 10 days, and in bryndza cheese produced from the curd, in th ... | 2014 | 24291178 |
| acute die-off of chamois (rupicapra rupicapra) in the eastern austrian alps due to bacterial bronchopneumonia with pasteurellaceae. | monitoring circulating pathogens in wildlife populations is important in evaluating causes and sources of disease as well as understanding transmission between wild and domestic animals. in spring 2010, a sudden die-off in a chamois (rupicapra rupicapra) population sharing habitat with livestock occurred in northeastern austria. nineteen animals were submitted for examination. necropsy and pathohistologic and bacteriologic results yielded lesions associated with pasteurellaceae species. addition ... | 2014 | 24807183 |
| phylogenetic and epidemiologic relationships among pasteurellaceae from colorado bighorn sheep herds. | we used 16s rrna sequencing and leukotoxin gene (lkta) screening via pcr assay to clarify phylogenetic and epidemiologic relationships among pasteurellaceae isolated from bighorn sheep (ovis canadensis). only six of 21 bighorn isolates identified as "mannheimia haemolytica" in original laboratory reports appeared to be isolates of m. haemolytica sensu stricto based on 16s rrna sequence comparisons; the remainder grouped with m. glucosida (n=8) or m. ruminalis (n=7). similarly, 16s rrna sequence ... | 2013 | 23778616 |
| the upper respiratory tract is a natural reservoir of haemolytic mannheimia species associated with ovine mastitis. | lamb suckling has been suggested to be an important way of infecting a ewe's udder with different bacteria, including mannheimia haemolytica. to test the potential role of lambs in transferring mannheimia species to the ewe's udder, the restriction endonuclease cleavage patterns of isolates obtained from nasopharyngeal swabs were compared with those obtained from cases of mastitis. sterile cotton swabs were used to collect nasopharyngeal samples from 50 ewes and 36 lambs from three flocks. m. ha ... | 2015 | 26542125 |
| sequence diversity, cytotoxicity and antigenic similarities of the leukotoxin of isolates of mannheimia species from mastitis in domestic sheep. | species within the genus mannheimia are among the most important causes of ovine mastitis. isolates of these species can express leukotoxin a (lkta), a primary virulence factor of these bacteria. to examine the significance of variation in the lkta, the sequences of the lkta genes in a panel of isolates from cases of ovine mastitis were compared. the cross-neutralising capacities of rat antisera raised against lkta of one mannheimia glucosida, one haemolytic mannheimia ruminalis, and two mannhei ... | 2014 | 25246232 |
| molecular epidemiology of mannheimia haemolytica and mannheimia glucosida associated with ovine mastitis. | while mannheimia haemolytica and mannheimia glucosida have been recognized as causes of intramammary infection in sheep, there has been no investigation of the epidemiology of the strains involved. pulsed field gel electrophoresis was used to study the molecular epidemiology of isolates of these 2 species associated with ovine mastitis. ten distinct strains were recognized among 12 m. haemolytica isolates, and 7 distinct strains among 13 m. glucosida isolates. the results demonstrate a high dive ... | 2012 | 22621951 |
| human wound infection with mannheimia glucosida following lamb bite. | mannheimia spp. are veterinary pathogens that can cause mastitis and pneumonia in domestic cattle and sheep. while mannheimia glucosida can be found as normal flora in oral and respiratory mucosa in sheep, there have been no reported cases of human infection with this organism. | 2015 | 26202121 |