| repertoire, genealogy and genomic organization of cruzipain and homologous genes in trypanosoma cruzi, t. cruzi-like and other trypanosome species. | trypanosoma cruzi, the agent of chagas disease, is a complex of genetically diverse isolates highly phylogenetically related to t. cruzi-like species, trypanosoma cruzi marinkellei and trypanosoma dionisii, all sharing morphology of blood and culture forms and development within cells. however, they differ in hosts, vectors and pathogenicity: t. cruzi is a human pathogen infective to virtually all mammals whilst the other two species are non-pathogenic and bat restricted. previous studies sugges ... | 2012 | 22685565 |
| phylogeographical, ecological and biological patterns shown by nuclear (ssrrna and ggapdh) and mitochondrial (cyt b) genes of trypanosomes of the subgenus schizotrypanum parasitic in brazilian bats. | the genetic diversity and phylogeographical patterns of trypanosoma species that infect brazilian bats were evaluated by examining 1043 bats from 63 species of seven families captured in amazonia, the pantanal, cerrado and the atlantic forest biomes of brazil. the prevalence of trypanosome-infected bats, as estimated by haemoculture, was 12.9%, resulting in 77 cultures of isolates, most morphologically identified as trypanosoma cf. cruzi, classified by barcoding using partial sequences from ssrr ... | 2010 | 19766649 |
| characterization of trypanosomes from the subgenus schizotrypanum isolated from bats, eptesicus sp. (chiroptera: vespertilionidae), captured in florianópolis, santa catarina state, brazil. | seven trypanosoma spp. isolates obtained from bats (eptesicus sp.) were characterized using experimental infection in mice, triatomines, and culicines; complement lysis; indirect fluorescence assays; as well as isoenzyme and random-amplified polymorphic dna (rapd) profiles. the trypanosoma sp. isolates were compared with trypanosoma cruzi, trypanosoma rangeli. and 2 other bat trypanosomes species, trypanosoma vespertilionis and trypanosoma hastatus. trypanosoma sp. isolates were different from t ... | 1998 | 9645864 |
| phylogenetic diversity of bat trypanosomes of subgenus schizotrypanum based on multilocus enzyme electrophoresis, random amplified polymorphic dna, and cytochrome b nucleotide sequence analyses. | trypanosome stocks isolated from bats (chiroptera) and belonging to the subgenus schizotrypanum were analyzed by multilocus enzyme electrophoresis (mlee) at 22 loci, random amplified polymorphic dna (rapd) with 14 primers and/or cytochrome b nucleotide sequence. bat trypanosomes belonged to the species trypanosoma cruzi marinkellei (10 stocks), trypanosoma dionisii (four stocks) and trypanosoma vespertilionis (three stocks). one t. rangeli stock and seven stocks of t. cruzi sensu stricto, the ag ... | 2003 | 12797982 |
| cytochrome c oxidase subunit 1 gene as a dna barcode for discriminating trypanosoma cruzi dtus and closely related species. | the dna barcoding system using the cytochrome c oxidase subunit 1 mitochondrial gene (cox1 or coi) is highly efficient for discriminating vertebrate and invertebrate species. in the present study, we examined the suitability of cox1 as a marker for trypanosoma cruzi identification from other closely related species. additionally, we combined the sequences of cox1 and the nuclear gene glucose-6-phosphate isomerase (gpi) to evaluate the occurrence of mitochondrial introgression and the presence of ... | 2017 | 29037251 |
| identification of bat trypanosomes from minas gerais state, brazil, based on 18s rdna and cathepsin-l-like targets. | several bat species can be infected by trypanosomes, but there is not much information about which of these parasites infect bats from triângulo mineiro and alto paranaíba, minas gerais state, brazil, a formerly endemic region for trypanosoma cruzi, the causative agent of chagas disease. the aim of this study was to describe, characterize, and identify the presence of trypanosomes in bats. the captured bats (448) belong to four families and to 19 different species. of those, 37 bats were found t ... | 2018 | 29340783 |
| isolation and phylogenetic relationships of bat trypanosomes from different biomes in mato grosso, brazil. | in the order chiroptera, more than 30 trypanosome species belonging to the subgenera herpetosoma, schizotrypanum, megatrypanum, and trypanozoon have been described. the species trypanosoma cruzi , trypanosoma cruzi marinkellei, and trypanosoma dionisii are the most common in bats and belong to the schizotrypanum subgenus. bats from 2 different biomes, pantanal and amazonia/cerrado in the state of mato grosso, brazil, were evaluated according to the presence of trypanosome parasites by means of h ... | 2013 | 23859496 |
| phylogenetic analysis of bolivian bat trypanosomes of the subgenus schizotrypanum based on cytochrome b sequence and minicircle analyses. | the aim of this study was to establish the phylogenetic relationships of trypanosomes present in blood samples of bolivian carollia bats. eighteen cloned stocks were isolated from 115 bats belonging to carollia perspicillata (phyllostomidae) from three amazonian areas of the chapare province of bolivia and studied by xenodiagnosis using the vectors rhodnius robustus and triatoma infestans (trypanosoma cruzi marenkellei) or haemoculture (trypanosoma dionisii). the pcr dna amplified was analyzed b ... | 2012 | 22590570 |
| comparative genomic analysis of human infective trypanosoma cruzi lineages with the bat-restricted subspecies t. cruzi marinkellei. | trypanosoma cruzi marinkellei is a bat-associated parasite of the subgenus schizotrypanum and it is regarded as a t. cruzi subspecies. here we report a draft genome sequence of t. c. marinkellei and comparison with t. c. cruzi. our aims were to identify unique sequences and genomic features, which may relate to their distinct niches. | 2012 | 23035642 |
| trypanosoma cruzi and leishmania infantum chagasi infection in wild mammals from maranhão state, brazil. | trypanosoma and leishmania are obligate parasites that cause important diseases in human and domestic animals. wild mammals are the natural reservoirs of these parasites, which are transmitted by hematophagous arthropods. the present study aimed to detect the natural occurrence of trypanosomatids through serological diagnosis, pcr of whole blood and blood culture (hemoculture), and phylogenetic relationships using small subunit ribosomal dna (ssu rdna), cytochrome b, and glycosomal glyceraldehyd ... | 2015 | 26501369 |
| survey of trypanosoma and leishmania in wild and domestic animals in an atlantic rainforest fragment and surroundings in the state of espírito santo, brazil. | trypanosoma and leishmania infections affect wild and domestic animals and human populations. the growing process of deforestation and urbanization of atlantic rainforest areas has given rise to introduction of humans and domestic animals to the sylvatic cycles of trypanosoma and leishmania species. serological, parasitological, and molecular surveys among wild and domestic animals in the corrego do veado biological reserve, which is an atlantic rainforest fragment in the state of espírito santo ... | 2014 | 24897863 |
| small subunit ribosomal metabarcoding reveals extraordinary trypanosomatid diversity in brazilian bats. | bats are a highly successful, globally dispersed order of mammals that occupy a wide array of ecological niches. they are also intensely parasitized and implicated in multiple viral, bacterial and parasitic zoonoses. trypanosomes are thought to be especially abundant and diverse in bats. in this study, we used 18s ribosomal rna metabarcoding to probe bat trypanosome diversity in unprecedented detail. | 2017 | 28727769 |
| the driving of immune response by th1 adjuvants in immunization of mice with trypanosoma cruzi marinkellei elicits a controversial infection control. | in previous studies, we have demonstrated that inoculation with a trypanosoma cruzi marinkellei (avirulent rm1 strain) was able to reduce parasitemia in mice challenged with t. cruzi, although it was not able to prevent histopathological lesions. th1 response stimulation by immunization is necessary for t. cruzi infection control, but the resistance is also dependent on immunoregulatory mechanisms, which can be induced by adjuvants. thus, we evaluated whether inoculation of t. cruzi marinkellei ... | 2016 | 26959861 |
| the diversity and expansion of the trans-sialidase gene family is a common feature in trypanosoma cruzi clade members. | trans-sialidase (ts) is a polymorphic protein superfamily described in members of the protozoan genus trypanosoma. of the eight ts groups recently described, ts group i proteins (some of which have catalytic activity) are present in the distantly related trypanosoma brucei and trypanosoma cruzi phylogenetic clades, whereas other ts groups have only been described in some species belonging to the t. cruzi clade. in the present study we analyzed the repertoire, distribution and phylogenetic relati ... | 2016 | 26640033 |