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digestive enzymes of three teleost fishes.the activity of amylase, sucrase, protease and lipase has been examined in wallago attu, clarias batrachus and labeo rohita. the optimum ph value for carbohydrases ranges from 5.0 to 7.0 and that for trypsin between ph 6.8 and 7.8. lipase is active at a slightly more alkaline medium. the optimum ph for a given enzyme varies in different sections of the alimentary canal of the same fish and also from species to species. variations are also found in the optimum substrate concentration for a given ...197514477
studies on vertebrate kidney. v. pas-positive lipids in kidneys of fishes from different habitats.five fish species living in different habitats, i.e. fresh water, estuarine and marine, were studied for the distribution of pas-positive materia in various regions of the kidney, 10 minutes' oxidation with 0-5 per cent. hio4 being employed prior to treatment with schiff's reagent. pas-positive material was detected in different sites of the kidney, i.e. brush border of proximal tubules, proximal tubule cells' cytoplasm, distal tubule cells' cytoplasm, glomerulus, basal cell border of proximal t ...197553169
pituitary cytology of the indian carp labeo rohita (ham.).in the adenohypophysis of labeo rohita eight cell types could be identified on the basis of their different tinctorial response to various staining techniques. the rostral pars distalis has 3 types of cells. type 1 is stained with erythrosin, orange g, and acid fuchsin. type 2 cells are lead hematoxylin positive and give an amphiphilic reaction in response to tri and tetrachrome techniques. type 3 cells are stainable with pas, ath, ab, af, and aniline blue and are distributed in an area comprise ...197776451
seasonal changes in the blood parameters of two major carps, labeo rohita (ham.) and cirrhina mrigala (ham.).seasonal changes in blood properties of two indian major carps, l. rohita and c. mrigala, were investigated. haematocrit values, haemoglobin concentrations and erythrocyte numbers showed seasonal fluctuations, being high from september to november and again in april-may, and low from january to march. males had generally higher blood values than the females.197989995
pesticide-induced alterations in the oxygen uptake rate of a freshwater major carp labeo rohita. 1979119624
in vitro disruption of atp dependent active transport following treatment with aldrin and its epoxy analog dieldrin in a fresh water teleost, labeo rohita.tissue homogenates of brain, gill, liver and kidney of labeo rohita were subjected in vitro to the various concentrations as 5.00, 1.66, 0.55, 0.18 and 0.06 mu m of 2 organochlorine pesticides aldrin and dieldrin and the disruption of atp dependent active transport (involving atpase) was studied. the inhibition of total atpase, mg2+ and na+, k+-dependent atpase by both the pesticides was quite similar and there was an increased inhibition with increased concentration of the toxicant, however, at ...1978152486
in vivo effect on atpase in certain tissues of labeo rohita and saccobranchus fossilis, following chronic chlordane intoxication. 1978155483
sensitivity of atpase system in certain tissues of labeo rohita and saccobranchus fossilis following in vitro treatment with chlordane. 1979159816
fungal infection of the young hatchlings of labeo rohita (ham.). 1979431559
physiological dysfunction of the haemopoietic system in a fresh water teleost, labeo rohita, following chronic chlordane exposure. part i--alterations in certain haematological parameters. 1979486769
physiological dysfunction of the haemopoietic system in a fresh water teleost, labeo rohita, following chronic chlordane exposure, part ii -- alterations in certain organic components and serum electrolytes. 1979486770
adrenal histochemistry of the freshwater teleost labeo rohita (ham.). 1979554564
hypothalamo-hypophysial neurosecretory and vascular system of the teleost labeo rohita (ham.).the paired nucleus preopticus (npo) lying on either side of the iii ventricle gives rise to the left and right main neurosecretory tracts which proceed posteroventrally towards the pituitary and give rise to several finer axons which ramify the infundibular floor adjacent to the pituitary. the bulk of the neurosecretory tracts enter the pars intermedia and few finer ones are seen among the pars distalis. the ventromedian component of the nucleus lateralis tuberis (nlt) is aldehyde fuchsin (af) p ...1977610488
a histochemical study of the mucous cells in the bucco-pharyngeal region of four indian freshwater fishes in relation to their origin, development, occurrence and probable functions.observations on the origin, development and occurrence of mucous cells in the bucco-pharyngeal region of labeo rohita (ham.), cirrhinus mrigala (ham.), catla catla (ham.) and clarias batrachus (linn.) have been made histochemically, by pas technique. the mucous cells have been found to develop from ordinary epithelial cells of the stratum malpighii which is situated immediately above the basement membrane. the different stages in the development of mucous cells are: 1. formation stage: some of t ...1975811054
development of the weberian apparatus in indian major carp labeo rohita (ham.).1. the lateral processes of the first two vertebrae are developed from the ossification of the mesenchyme. 2. the centra of the second and third vertebrae are fused together forming a composite structure. 3. the "transverse processes" of the fourth vertebrae are homologous with the pleural ribs. 4. the ossa suspensoria are developed from the lateral and ventromesial processes from the basiventrals and form a kind of arch over the dorsal aorta. 5. the dorsal cartilaginous mass arching over the an ...1977907185
seasonal changes in the histology of the pituitary gland of labeo rohita (ham.), an indian freshwater major carp, in relation to it's spawning behavior. 1977914000
the morpho-histology of the pituitary glands in two freshwater major carps of india, labeo rohita (ham.) and cirrhinus mrigala (ham.).1. the pituitary gland in labeo rohita and cirrhinus mrigala is a small, pear-shaped, whitish, soft body situated in a cavity -- the sella turcica. the gland is of the lepto-dorso basic type. 2. it consists of the neurohypophysis and adenohypophysis. the latter comprises three lobes viz., the rostral pars distalis, the proximal pars distalis and the pars intermedia. 3. the neurohypophysis branches into the pars distalis, extends into the pars intermedia and ramifies extensively. it is composed o ...1976984440
functional morpho-histology of the alimentary canal of an indian frewhwater major carp, labeo rohita (hamilton) during it's different life-history stages.1. the alimentary canal of the fry of l. rohita (hamilton) is of the carnivorous type in nature and disposition with a low relative length of the gut (r. l. g.). 2. end-buds are present. the buccopharyngeal region has a simple epithelial surface. mucous cells are small and few throughout the length of the alimentary canal. the presence of these structures in association with a zooplankton feeding habit indicate that the fries are primarily sightfeeders and carnivorous. 3. with increase in age an ...19751217739
comparative histomorphology of the mauthner cells in some freshwater teleosts.the histomorphological observations are made on the mauthner cells in eight species of teleosts belonging to six different families. the cells are better developed in channa punctatus, heteropneustes fossilis, labeo rohita, danio, malabaricus and puntius ticto. they are symmetrically situated in nandus nandus and are found to be absent in mastocembalus armatus. their position, shape and size vary in different species. the axon cap is well developed in channa punctatus, heteropneustes fossilis an ...19751234388
malathion induced changes in the optomotor behavior of an indian carp, labeo rohita. 19921421850
demonstration of two forms of vitellogenin in serum of estradiol-17 beta-treated indian major carp, labeo rohita.vitellogenin (vg) synthesis was induced in the male and non-vitellogenic female rohu, the indian major carp, by estradiol-17 beta(e2) where effect was more in female. a crude preparation of vg was isolated in the second peak after gel filtration on ultrogel aca 34 from the sera of vitellogenic female rohu and e2-treated male and female rohu. estimation of alkali-labile phosphorus was shown to be used as an index of vg. native-page analysis has revealed the presence of two forms of vg (vg1: 430,0 ...19921506024
a cost benefit analysis of fish culture strategy towards the control of mansonioides in shertallai, kerala state.the cost benefit analysis of fish culture as a means for vector control towards the containment of brugian filariasis in shertallai, a highly endemic tract for this disease in kerala was carried out. while the financial gain accrued to the community was to the tune of rs. 1,82,052 in ponds (under monoculture) with chinese grass carp from 763 ponds, it was rs. 1,83,740 from 1000 ponds under polyculture with catla catla, cyprinus carpio, labeo rohita, labeo fimbriatus, cirrhina mrigala and ctenoph ...19921506068
ontogenetic and comparative analysis of ldh isozymes in the three species of indian major carp.expression patterns of lactate dehydrogenase (ldh) isozyme were investigated in embryonic and post embryonic stages of labeo rohita, cirrhinus mrigala and catla catla using starch gel electrophoresis. species specific and differential enzyme locus (gene) expression patterns were found in ldh up to 18th hr of study. the isozyme up to 36th hr after fertilization seemed to be completely active and showed electrophoretic patterns very similar to those of the adults. comparative analysis of the isozy ...19921521871
copper influenced changes of lipid metabolism in the tissues of freshwater teleost labeo rohita (hamilton).modulations in the lipid metabolism in the gill, liver, muscle and brain of freshwater teleost labeo rohita exposed to 1, 2 and 3 days to lethal (1.2 mg/l) and 1, 15 and 30 days to sublethal (0.24 mg/l) concentrations of copper were studied. the total lipids decreased and there was an increase in the free fatty acids, glycerol and lipase activity in the organs studied at lethal concentration of copper. the degree in these shifts increased over time of exposure (1 less than 2 less than 3 days). i ...19921558545
perturbations in nitrogen metabolic profiles in the tissues of fish, labeo rohita exposed to fenvalerate.changes in the levels of nitrogen metabolic profiles in gill, brain, liver and muscle tissues of fish, labeo rohita exposed to sublethal concentration (10 micrograms/l) of fenvalerate for 6, 12, 24 and 48 hours were studied. ammonia content was decreased, whereas urea and glutamine levels were increased in the tissues of fenvalerate exposed fish. changes were more pronounced with an increase in the period of exposure. within the tissues, the alterations in excretory products were in the followin ...19911805800
seasonal variation in the occurrence of pallisentis ophiocephali and acanthosentis betwai (acanthocephala) in relation to their fish hosts.male and female channa striatus (bl.) and labeo rohita (ham.) were examined monthly for pallisentis ophiocephali (thapar, 1930) and acanthosentis betwai (tripathi, 1959) infections for a period of two years. c. striatus showed greater proportion of infection with p. ophiocephali in march to may and males were significantly more infected than females. l. rohita showed greater infection with a. betwai in june and july and sexes were not differentially affected. the frequencies of male and female p ...19911928802
evidence for melanin concentrating hormone (mch) receptors mediating melanosome aggregation in labeo melanophores.melanin concentrating hormone (mch: 5 x 10(-12)-5 x 10(-8) m) induced a concentration related, rapid and reversible pigment aggregation in innervated melanophores of labeo rohita. in inducing melanosome aggregation mch was found to be 10(4) times more potent than norepinephrine. experiments employing phentolamine and propranolol suggest that mch acts through its own specific receptors on the melanophores unrelated to adrenoceptors. mch was able to aggregate the melanosomes even in the absence of ...19901962758
pseudocryptogonimus pakistanensis n. gen., n. sp. (trematoda: cryptogonimidae) from the fish labeo rohita.pseudocryptogonimus pakistanensis n.g., n. sp., is described from freshwater fish labeo rohita from kalri lake, sind, pakistan. it is characterised by possessing spines at the basal margin of oral sucker; testes, postequatorial, subsymmetrical; vitellaria lateral to ovary in middle of hindbody, confluent in postovarian region and reaching to level of testes; ovary flattened; genital pore antero-lateral to acetabulum; seminal vesicle large and ejaculatory duct long. subfamily neochasminae van cle ...19902337253
the sympathetic neuro-melanophore transmission in a fresh-water indian major carp, labeo rohita (ham.).adrenaline was effective in aggregating the melanosomes both in innervated as well as denervated melanophores. isotonic kcl could induce pigment aggregation only in innervated melanophores. adrenaline- and k+-induced pigment aggregation response in these melanophores was blocked by phentolamine: propranolol failed to do so. it is suggested that the chromatic nerves in the fish, labeo rohita are adrenergic and via post-synaptic alpha-adrenoceptors, control the melanosome aggregation.19892550366
responses induced by long-term toxic effects of heavy metals on fish tissues concerned with digestion, absorption, and excretion.the present study relates to the bioassay as well as the responsiveness induced by long-term exposure to sublethal concentrations of soft acceptor heavy metals, lead (pb, iv a) and cadmium (cd, ii b), in various fish tissues viz., stomach/intestinal bulb, intestine, liver, pancreas, and kidney of mystus vittatus (bloch) and labeo rohita (hamilton) belonging to 2 different trophic strata. responses, however, have been recorded by histopathological and histochemical studies on the aforesaid tissue ...19892591700
in vitro effect of mammalian pituitary hormones on germinal vesicle breakdown in oocytes of major carps, labeo rohita, cirrhinus mrigala, catla catla and cyprinus carpio.among all the mammalian pituitary hormones, luteinizing hormone (lh) was the most potent in vitro inducer of oocyte maturation in l. rohita, c. mrigala, c. catla and c. carpio. it induced significant germinal vesicle breakdown (gvbd) at concentrations of 10, 1, 0.1 and 0.01 micrograms/ml. at the highest concentration used, lh induced 77.9 +/- 5.9, 73.8 +/- 4.6, 50.3 +/- 2.8 and 40.8 +/- 1.4% gvbd in oocytes of l. rohita, c. mrigala, c. catla and c. carpio, respectively. among other hormones, fol ...19892632393
histological changes in relation to accumulation and elimination of inorganic and methyl mercury in gills of labeo rohita hamilton.l. rohita was exposed to identical concentrations of inorganic and methyl mercury (hgcl2 and ch3hgcl) and the gills were studied for mercury bioaccumulation and histological changes. in methyl mercury exposed group the mercury level in the gills continuously increased til the end of the exposure period whereas the level started decreasing from the day 30 onwards in the other group even though the exposure was continued for 60 days. histological changes were similar in inorganic and methyl mercur ...19892807407
in vitro effects of cyanoketone and epostane on lh-induced germinal vesicle breakdown in oocytes of indian major carps.the effect of cyanoketone (inhibitor of delta 5-3 beta-hydroxysteroid dehydrogenase) and epostane (inhibitor of 3 beta-hydroxysteroid dehydrogenase) on lh-induced in vitro germinal vesicle breakdown (gvbd) in the oocytes of indian major carps, labeo rohita, cirrhinus mrigala, and catla catla, was investigated using five concentrations for each inhibitor. both of these inhibitors could significantly inhibit the lh-induced gvbd at all of their concentrations in l. rohita and c. catla. however, in ...19892920902
biochemical compartmentation of gluconeogenic enzymes in fish tissues.compartmentation of liver, kidney muscle and gill tissues in relation to glucose-6-phosphatase and fructose 1,6-diphosphatase was examined in the fishes labeo rohita, clarias batrachus and channa punctatus. the anterior region of the right and left lobes of the liver contained the maximum of fructose 1,6-diphosphatase and glucose-6-phosphatase, while the minimum was in the right and left lobes of gill tissue. herbivore fish had the highest gluconeogenic enzyme content followed by carnivore and p ...19863030045
differential distribution of hexokinase in vital organs of three teleost fishes.the phylogeny has influenced the distribution of hexokinase in various tissues of three teleosts, viz., labeo rohita, clarias batrachus and channa punctatus, which differ significantly in a number of parameters like growth rate, feeding habits and ability to get oxygen. among the organs investigated for hexokinase, the anterior region of the right lobe of the liver showed higher activity than the posterior region, followed by the anterior and posterior regions of the left lobe. the middle region ...19883250207
annual cyclical changes in the testicular activity of an indian freshwater major carp, labeo rohita (hamilton).different male germ cells identified on the basis of histological and cytological characteristics in the testicular lobules of labeo rohita have been grouped into spermatogonia, primary spermatocytes, secondary spermatocytes, spermatids and spermatozoa. the seasonal changes of the testis in l. rohita have been described according to its morphological peculiarities as well as to its variations in gonadal volumes, gsi values and frequency percentages of the different male germ cells occurring in t ...19863710120
toxicity of four pesticides on the fingerlings of indian major carps labeo rohita, catla catla, and cirrhinus mrigala.fingerlings of labeo rohita, catla catla, and cirrhinus mrigala were exposed to selected doses of four commonly used pesticides carbofuran, ddt, dimethoate, and meta-systox for a period up to 30 days to determine relative toxicological effects, lc50 values for 96 hr, maximum acceptable tolerant concentration, and application factor. the use of early life history tests has been emphasized for toxicological assessments.19863792265
distribution of phospholipids in the various organs of labeo rohita. 19704319618
yeast dosage level and the postembryonic survival and growth of the carp labeo rohita--ham. 19655884315
sulfomucins and sialomucins in the mucus cells of the alimentary canal in an indian freshwater major carp, labeo rohita (hamilton): a fluorescence microscopic study.the localization of sulfomucins and sialomucins present in the various types of mucus cells--secretory (smc) and mature non-secretory mucus cells (mmc) of the different regions of the alimentary canal in labeo rohita (ham.) has been studied by fluorescence microscopical techniques (viz., ferric alum coriphosphine--fac, coriphosphine thiazol yellow--cty and dische reaction--dr). high degree of fluorescence activity confirms the presence of relatively high amount of sulfomucins in the mucus cells ...19836199266
atpase activity of myofibrillar proteins of rohu extracted at temperatures between 15 & 18 degrees c. 19836231238
ddt toxicity: gluconeogenic enzymes and non-specific phosphomonoesterases in three teleosts.in 3 fish species (labeo rohita, clarias batrachus and channa punctatus) subjected to a sublethal concentration (0.035 mg/l) of ddt continuously for 20 days both hepatic and renal acid and alkaline phosphatases were elevated, whereas the rise in glucose-6-phosphatase and fructose-1,6-diphosphatase was restricted to hepatic tissue. the variations in phosphatase levels were more pronounced in l. rohita than in the other two species.19826294922
lipid-protein interaction causing denaturation of frozen rohu myofibrillar proteins--probable role of cryoprotective agents. 19846526417
scanning electron microscopic study of the intestinal mucosa of an indian freshwater adult major carp, labeo rohita (hamilton).fine anatomical structures of the different regions (viz., anterior, middle and posterior) of the intestinal mucosa in adult labeo rohita (hamilton) have been studied by using scanning electron microscope (sem). surface mucosal modifications of the anterior and the middle of the intestine not only increase the luminal surface by 2-4 times but serve also the storage function of the ingested food due to the absence of stomach in the fish studied. fine anatomical structures of the columnar epitheli ...19836670351
acute toxicity of vegetable oil factory effluent to some freshwater teleosts in relation to size.specimens of cirrhina mrigala (ham.), labeo rohita (ham.) and channa punctatus (bl.) falling in the size (total length) range of 5.5 +/- 1.0 cm to 27.0 +/- 2.0 cm were exposed to different concentrations of the vegetable oil factory effluent for evaluating the influence of size on the acute toxicity of the factory effluent. the results suggest that relative toxicity of the effluent decreased with increase in the size of the fish. however, for specimens exceeding 20.0 +/- 1.6 cm in size, the toxi ...19846719496
toxicity of carbaryl & 1-naphthol to the freshwater fish labeo rohita. 19806772552
haematological and haemopoietic studies in an air-breathing fish on cyanocobalamin and folacin deficient diet.experimental deficiencies of cyanocobalamin and folacin separately and in combination were induced in an air-breathing teleost fish channa punctatus with the help of a complete vitamin test diet. cyanocobalamin deficiency produced normocytic hypochromic anaemia while the folacin deficiency as well as the combined deficiency of both vitamins produced macrocytic hypochromic anaemia. leucocytosis was observed in the individual and combined deficiencies of these vitamins with significant increase in ...19817346998
size related toxicity of copper & mercury to lebistes reticulatus (peter), labeo rohita (ham.) & cyprinus carpio linn. 19807399616
relative toxicity of endosulfan, its isomers, and formulated products to the freshwater fish labeo rohita.the relative toxicity of endosulfan, its isomers, and formulated products to the freshwater fish labeo rohita was tested. the 96-h lc50 values showed that toxicity decreased in the following order: isomer a, 35% emulsifiable concentrate, technical endosulfan, 4% dust, and isomer b. the principal method of detoxification was by formation of endosulfan ether in the liver, endosulfant, at both sublethal and lethal doses, enhanced oxygen consumption in l. rohita. histological studies showed minor ch ...19807420483
composite fish culture for mosquito control in rice fields in southern india.composite culture of edible fishes (common carp, cryprinus carpio; silver carp, hypopthalmithys molitrix, grass carp, ctenopharyngodon idella; catla, catla catla; rohu, labeo rohita; and mrigal, cirrhinus mrigala) in rice fields in the cauvery delta of tamil nadu, southern india, resulted in 81.0% reduction in the immature mosquito population of anophelines and 83.5% of culicines. analysis of fish feces for mosquito larval head capsules showed that common carp and silver carp are effective larvi ...19947777919
comparison of the sublethal effect of mercury and lead on visceral dehydrogenase system in three inland teleosts.the sublethal effect of mercury and lead was investigated on visceral (liver, muscle, gill, kidney and brain) succinic, malic and lactic dehydrogenases in labeo rohita, clarias batrachus and chana punctatus in acute experiments. the highest decrease of succinic, malic and lactic dehydrogenases was recorded in the hepatic tissue in comparison to muscle, brain, kidney and gill. this decrease was greater in l. rohita than in c. batrachus or in c. punctatus. mercury was more effective than lead. mar ...19938329378
cypermethrin-induced in vivo alterations in the carbohydrate metabolism of freshwater fish, labeo rohita.aspects of carbohydrate metabolism under in vivo conditions were analyzed in functionally different tissues of the freshwater fish, labeo rohita, exposed to a lethal (lc50/96 hr = 5.24 micrograms liter-1) and sublethal concentration (0.52 micrograms liter-1) of cypermethrin for 4 days. all exposed fish exhibited a hyperglycemic condition. an increase in tissue lactate with a decrease in pyruvate, total carbohydrates, and glycogen contents was noted. activity of lactate dehydrogenase was elevated ...19958521784
acute renal failure associated with freshwater fish toxin.clinical observations on toxicity after ingestion of gall bladder of labeo rohita, a freshwater fish found commonly in india, were recorded from 22 patients between 1985 to 1990. the gall bladder in raw, cooked or desiccated form was swallowed as a traditional method of treatment for various chronic diseases. patients generally presented with gastrointestinal symptoms such as cramping pain, nausea and vomiting within 12 hours (mean 4.6 +/- 3.7 hours) after ingestion. subsequently renal failure w ...19958592773
sublethal effect of mercury and lead on monoamine oxidase in different regions of the brain in three freshwater teleosts.effect of mercury and lead at 24 and 48 h was investigated on monoamine oxidase (mao) activity in different regions of the brain (telencephalon, cerebellum, diencephalon and medulla oblongata) in labeo rohita, ham., clarias batrachus, l. and channa punctatus, bloch. highest rise in mao activity was recorded in telencephalon with mercury followed by lead. maximum variations in the level of mao activity in different regions of the brain were recorded at 24 h exposure. the observed alterations were ...19958606989
role of phosphatases during transport and energy metabolism in labeo rohita after exposure to cypermethrin.freshwater fish, labeo rohita, were exposed to sublethal concentration (0.5 micrograms.l-1) of cypermethrin for 7 and 15 days to examine the bioenergetics in functionally four different tissues, namely, gill, liver, brain and muscle. whole animal oxygen consumption was measured first and it was found to decrease in both the exposure periods (eps), manifesting respiratory distress of the animal in toxic environment. ionic regulation and energy requirements were also found to be altered under stre ...19968721628
microheterogeneity of c-reactive protein in the sera of fish labeo rohita induced by metal pollutants.a group of c-reactive proteins (crps) have been purified to apparent homogeneity by calcium (ca++) dependent affinity chromatography on phosphoryl choline sepharose column from the sera of l. rohita confined in normal and nonlethal doses of cadmium (cd++) and mercury (hg++) polluted water. the crps levels are elevated in the serum of both cd++ and hg++ polluted fishes. all three crps are glycoproteins having identical subunits of mr 33,000. the total content of carbohydrate are 21.4%, 16.4% and ...19968831675
a cell line from the gill tissues of indian cyprinoid labeo rohita. 19979201509
immunization with bacterial antigens: infections with motile aeromonads.motile aeromonads are a heterogeneous group of organisms which are involved in a number of disease syndromes of warm water fish. they are commonly associated with bacterial haemorrhagic septicaemia, infectious dropsy, red mouth disease, ulcerative conditions etc. a variety of factors has been considered to be associated with virulence including haemolysins, proteases, surface array protein, acetylcholinesterase etc. we have studied the immune response of indian major carps to antigens of motile ...19979270842
characterization of mboi satellites in cirrhina mrigala and clarias batrachus (pisces).we have cloned and characterized two highly reiterated, tandemly repeated, and a+t rich mboi dna fragments, one in cirrhina mrigala (cyprinidae), with a monomer size of 266 bp, and one in clarias batrachus (clariidae), with a monomer size of 227 bp. the mboi fragment in c. mrigala is species-specific and absent in other carps, such as catla catla and labeo rohita. the mboi fragment in c. batrachus was also present in two other catfishes tested, namely clarias gariepinus and heteropneustes fossil ...19989549057
vitamin d is not an essential nutrient for rora (labeo rohita) as a representative of freshwater fish.this investigation was directed towards finding the need of vitamin d for fish. the freshwater column feeder fish labeo rohita (rora) was used for the study. early fry stage fish were divided into four experimental groups of 350 each: two groups were kept in natural light, while the other two were maintained under total darkness. one each of the light and dark-grown groups was supplied dietary vitamin d3 [1,650 i.u/kg diet], whereas the other groups were fed a vitamin d-deficient diet for six mo ...19989675700
effect of different concentrations of cryoprotectant and extender on the hatching of indian major carp embryos (labeo rohita, catla catla, and cirrhinus mrigala) stored at low temperaturerohu (labeo rohita), catla (catla catla) and mrigal (cirrhinus mrigala) embryos were examined for hatching success after short-term (8-13 h) storage at low temperature (4 degreesc) using three concentrations of three cryoprotectants, methanol (1, 2, and 3 m), dimethyl sulfoxide (1.282, 1.923, and 2.564 m, equal to 10, 15, and 20%), and glycerol (1.087, 1.63, and 2.174 m, equal to 10, 15, and 20%), separately as well as together with a single concentration of sucrose (0.5 m). the aim was to deter ...19989917348
vitamin d2 is not biologically active for rora (labeo rohita) as vitamin d3.the present investigation was directed towards finding the relative biopotency of vitamin d3 and d2 in fish. the freshwater column feeder fish labeo rohita (rora) was used for the study. the feeding of rora with graded levels of vitamin d2 (550, 1,100 and 1,650 i.u./kg diet) and vitamin d3 (1,100 and 1,650 i.u./kg diet) resulted in no behavioural or morphological changes in comparison with the group fed a vitamin d-deficient diet. also, the growth rate, feed efficiency, mortality rate, carcass p ...199910360238
purification and characterization of fish liver ferritins.ferritin from the liver of fresh, salt and brackish water fishes was purified by thermal denaturation of liver homogenate followed by ammonium sulphate fractionation and sephacryl s-300 gel filtration. yield and iron content of purified fish ferritins were 0.016-0.026 mg/g of wet tissue and 4-14%, respectively. the iron content of ferritins from marine and brackish species was higher than from fresh water species. the phosphate/iron ratio ranged from 0.5 to 1.8 and was higher than mammalian ferr ...199910481257
bioassay of cadmium and its effect on differential distribution of dehydrogenases in different brain regions in labeo rohita (ham).the sublethal effect of cadmium on the specific activities of lactic, malic and succinic dehydrogenases in different brain regions in labeo rohita (ham) was assessed with reference to acute, chronic and recovery conditions. cadmium enhanced succinic, malic and lactic dehydrogenases to a marked extent in the cerebrum from 0 to 12 h exposure. however, a subsequent fall of the above enzymes in some regions was recorded from 12 to 24 h. in chronic studies, the greatest decrease in succinic dehydroge ...199910523059
chronic toxic effects of quinalphos on some biochemical parameters in labeo rohita (ham.).the effect of exposure to sublethal concentrations of the organophosphate pesticide, quinalphos (1.12, 0.22 mg/l) on biochemical parameters of muscle and enzyme activities in brain, liver and kidney of the indian major carp, labeo rohita was studied after 15, 30 and 45 days. the muscle protein and rna levels decreased whereas dna levels and acid phosphatase were elevated. similarly, alkaline phosphatase was depleted. the brain acetyl cholinesterase activity was decreased most (-75.43%) in 1.12 m ...200010713464
cloning of partial putative gonadotropin hormone receptor sequence from fish.a search for the presence of mariner-like elements in the labeo rohita genome by polymerase chain reaction led to the amplification of a partial dna sequence coding for a putative transmembrane domain of gonadotropin hormone receptor. the amplified dna sequence shows a high degree of homology to the available turkey and human luteinizing and follicle stimulating hormone receptor coding sequences. this is the first report on cloning such sequences of piscine origin.200010824197
lectin like properties and differential sugar binding characteristics of c-reactive proteins purified from sera of normal and pollutant induced labeo rohita.different forms of c-reactive proteins (crps) have been purified to electrophoretic homogeneity from the sera of labeo rohita confined in freshwater (crp(n)) and water polluted with nonlethal doses of cadmium (crp(cd)) or mercury (crp(hg)). crp(n), crp(cd), and crp(hg) show remarkable differences in their electrophoretic mobility but exhibit strong immunological cross reactivity. all these crps exhibit variable agglutination properties with erythrocytes from diverse sources in presence of ca+2, ...199911003559
bioconcentration of endosulfan and monocrotophos by labeo rohita and channa punctata. 200011014846
uptake and processing of biofilm and free-cell vaccines of aeromonas hydrophila in indian major carps and common carp following oral vaccination--antigen localization by a monoclonal antibody.uptake and processing of biofilm (bf) and free-cell (fc) vaccines of aeromonas hydrophila were studied in the indian major carps catla catla catla, and rohu labeo rohita and in the common carp cyprinus carpio following a single dose oral vaccination of 10(11) cfu g(-1) fish. fish were sampled at 0.5, 1, 3, 6, 12, 24 h and at 2, 3, 5 and 10 d following vaccination and antigen localization was studied in the gut, kidney and spleen employing monoclonal antibody based immunofluorescence and immunope ...200011145450
acute toxicity of cadmium to fish labeo rohita and copepod diaptomus forbesi pre-exposed to cao and kmno4.96-h lc50 values of cadmium (cd) to fish labeo rohita and the copepod diaptomus forbesi, determined by static bioassays, were, respectively, 89.5 and 10.2 mg/l. lc50 values increased significantly when fish pre-exposed to 100-350 mg/l cao or 0.5-1.5 mg/l kmno4 for 4 d and the copepod to 20-70 mg/l cao or 0.25-1.0 mg/l kmno4 for same period. the lc50 values also increased when the pre-exposure period of cao was increased to 12 d at concentration 100 mg/l for fish and 20 mg/l for copepod. all fish ...200111272918
copper-influenced changes in lactate dehydrogenase and g-6-pdh activities of the freshwater teleost, labeo rohita. 200111429684
immunosuppressive effects of aflatoxin b1 in indian major carp (labeo rohita).aflatoxin b1 (afb1) is a potent immunomodulator in endotherms. the experiment was carried out to study the immunosuppressive nature of afb1 in one ectothermic species of indian major carp. graded levels (0, 1.25, 5.00 mg/kg of body weight) of purified afb1 were intraperitoneally (i.p.) injected into rohu (labeo rohita) fingerlings weighing 30-50 g, and the fish were observed for a period of 90 days. at the end of the trial, blood samples were collected from the control group as well as the afb1 ...200111440188
influence of protective agents on metal induced respiratory distress in labeo rohita (ham). 200111443331
acute and subchronic toxicity of aflatoxin b1 to rohu, labeo rohita (hamilton).pathological alterations in various organs of rohu (l. rohita) fingerlings following acute (0, 7.50, 11.25 and 13.75 mg/kg body weight) and subchronic (0, 1.25 and 2.50 mg/kg body weight) single i.p. aflatoxin b1 exposure for 10 and 90 days, respectively, were investigated. mortality (dose-dependent) was marked only during acute toxicosis. the changes observed in various organs were dose and time dependent. the acute dose groups revealed toxic changes viz., necrotic and vascular changes in liver ...200111510129
growth performance of two indian major carps, catla (catla catla) and rohu (labeo rohita) fed diets containing different levels of spirulina platensis.the influence of spirulina platensis meal on the growth and carcass composition of two indian major carps, catla, catla catla and rohu, labeo rohita was investigated in a 90-day culture trial. four experimental diets were used and spirulina replaced fish meal protein from the standard diet at 25%, 50%, 75% and 100% levels. there was no significant difference in the final weight attained by catla at all levels of spirulina incorporation as compared to the fish-meal-based control diet. however, th ...200111563701
insulin alone can lead to a withdrawal of meiotic arrest in the carp oocyte.meiotic arrest of oocyte in an indian carp, labeo rohita ham. has been found for the first time to be withdrawn by insulin only. addition of insulin to oocytes in vitro caused germinal vesicle breakdown (gvbd), one of the first visual markers to determine initiation of the final maturational process. under the influence of insulin the germinal vesicle (gv) of the oocyte migrated towards the animal pole, reached the micropyle and then dissolved (gvbd). by using different concentrations of insulin ...200111568479
acute phase response of c-reactive protein of labeo rohita to aquatic pollutants is accompanied by the appearance of distinct molecular forms.different forms of c-reactive proteins have been purified to electrophoretic homogeneity by calcium dependent affinity chromatography on a phosphorylcholine (pc)-sepharose column from the sera of labeo rohita confined in fresh water (crp(n)) and water polluted with sublethal doses of cadmium (crp(cd)), mercury (crp(hg)), phenol (crp(ph)), and hexachlorocyclohexane (crp(hx)), which elevate serum crp levels by three- to fivefold. on native page, induced forms of crp show remarkable differences in ...200111747291
effect of dietary beta-1,3 glucan on immune responses and disease resistance of healthy and aflatoxin b1-induced immunocompromised rohu (labeo rohita hamilton).the immunostimulant beta-1,3 glucan was fed at 0.1% in feed for 7 days to healthy and aflatoxin b1 (afb1)-induced immunocompromised fish, labeo rohita (one of the major tropical carp species), in a 60 day trial. the effects of afb1, glucan and their interactions on non-specific and specific immunity levels and disease resistance of fish were studied. a single intraperitoneal injection of afb1 at 1.25 mg kg(-1) body weight) caused a significant (p < 0.05) reduction in non-specific immunity as mea ...200111759039
spawning behaviour of an air-breathing catfish heteropneustes fossilis (bloch).spawning behavior of h. fossilis was studied by breeding the fish in laboratory through hypophysation using pituitary glands of the indian major carps (catla catla and labeo rohita) at a dose of about 15 mg/100 g body weight of the recipient. the spawning activity started about 6 to 10 hr after the administration of pituitary injection. the activity lasted 2 to 6 hr in various experiments. during this period, intermittent mating acts were observed, the average rate of mating being once every 2 t ...197711803690
the effect of dietary immunomodulation upon edwardsiella tarda vaccination in healthy and immunocompromised indian major carp (labeo rohita).in order to determine the impact on disease resistance of four dietary immunomodulators viz., beta-1,3 glucan, levamisole, vitamins c and e, in an important farmed indian major carp species, rohu (labeo rohita ham.), fish were fed diets containing various levels of these substances during a 60 day trial. aflatoxin b1 (afb,) at 125 mg kg(-1) body weight was injected intraperitoneally (i.p.) into fish to induce an immunosuppressive state on the first day of the experiment in some individuals. the ...200211866127
molecular cloning of growth hormone-encoding cdna of an indian major carp, labeo rohita, and its expression in escherichia coli and zebrafish.the cdna clones encoding for growth hormone (gh) of an indian major carp rohu labeo rohita were isolated from a cdna library constructed from the poly(a)(+) rna extracted from the pituitary glands of rohu. partial gh cdna of the rohu (3'-end) was amplified by rt-pcr and used as probe to screen the cdna library. full-length gh-specific cdna clones (1180 bp) were isolated and sequenced. the sequence contains 48-bp 5'-noncoding region followed by an orf of 621 bp and a 3'-noncoding region of 521 bp ...200211884069
production of superoxide anion by head-kidney leucocytes of indian major carps immunised with bacterins of aeromonas hydrophila.on contact with micro-organisms or any other antigenic particles which are recognised as phagocytosable, the leucocytes of vertebrates raise their oxygen consumption suddenly (respiratory burst). the consumed oxygen is then converted into reactive oxygen species (ros). an effort has been made in the present study to estimate the production of superoxide anion, one of the ros, by the resident as well as activated head-kidney leucocytes of indian major carps. activation was accomplished by immunis ...200211931016
toxicity and residue studies of fenvalerate to some selected freshwater fishes.the toxicity test of fenvalerate were conducted to fresh water fishes labeo rohita (hamilton), catla catla (hamilton), cirrhinus mrigala (hamilton), aplocheilus punchex (hamilton) and ctenopharygodon idellus (valenciennes). the static and continuous flow through tests were employed to determine the lethal concentration (lc50) for 24h, 48h and 96h to the test fish. it was observed that cirrhinus mrigala is more sensitive to this pesticide followed by applochielus punchex, lebeo rohita, catla catl ...200112017257
effect of water extract of the bark of buchanania lanzan linn. on behaviour and chromatophores of a fresh water fish, labeo rohita.fish exposed to 150 ppm and 250 ppm doses saparately, showed eratic swimming with hyper-excitability, spiralling, convulsion and mortality. however at 350 ppm dose fish became lethargic and steady at the bottom of aquarium. this behaviour appears to be due to sedative effects of fish implicating the phychoactive nature of the plant b. ianzan. at 150 ppm to 350 ppm doses, fish scale changed from reticulate to punctate chromatophores; and treated fish when transferred to fresh water, were slowly r ...200112017267
haematological investigations on fishes infested with fungal growth.considerable changes have been observed in the haematological parameters of labeo rohita, mystus cavasius, nandus nandus and puntius sarana infested with aphanomyces sp., saprolegnia parasitica, achlya prolifera and dictyuchus monosporus, respectively. substantial decline have been observed in the number of erythrocytes and lymphocytes. the number of granulocytes and monocytes were noticed significantly increased. the amount of haemoglobin has also been decreased drastically in all the cases.200112018597
molecular cloning of growth hormone encoding cdna of indian major carps by a modified rapid amplification of cdna ends strategy.a modified rapid amplification of cdna ends (race) strategy has been developed for cloning highly conserved cdna sequences. using this modified method, the growth hormone (gh) encoding cdna sequences of labeo rohita, cirrhina mrigala and catla catla have been cloned, characterized and overexpressed in escherichia coli. these sequences show 96-98% homology to each other and are about 85% homologous to that of common carp. besides, an attempt has been made for the first time to describe a 3-d mode ...200212089475
duckweed (lemna polyrhiza) leaf meal as a source of feedstuff in formulated diets for rohu (labeo rohita ham.) fingerlings after fermentation with a fish intestinal bacterium.eight isonitrogenous (35% crude protein approximately) and isocaloric (4.2 kcal g(-1) approximately) diets were formulated including raw and fermented duckweed (lemna polyrhiza) leaf meal at 10%, 20%, 30% and 40% levels. a particular bacterial strain (bacillus sp.) isolated from carp (cyprinus carpio) intestine and having extracellular amylolytic, cellulolytic, proteolytic and lipolytic activities was used for leaf meal fermentation for 15 days at 37 degrees c. the fibre content of leaf meal red ...200212146637
immunisation of indian major carps against aeromonas hydrophila by intraperitoneal injection.the indian major carps catla catla, labeo rohita and cirrhinus mrigala were immunised against the potent bacterial fish pathogen, aeromonas hydrophila by the intraperitoneal route, in field conditions. two different polyvalent antigen preparations namely, whole cell and extracellular products (ecp) were used. immunisation schedule consisting of a single booster dose given 28 days after priming resulted in a good agglutinating antibody response in the carps. kinetics of the response were similar ...200212201649
efficacy of water hyacinth compost in nursery ponds for larval rearing of indian major carp, labeo rohita.the use of water hyacinth compost as manure in nursery ponds for larval rearing of indian major carp, labeo rohita was studied. better performance with regard to growth and survivability of the larvae was recorded in the ponds treated with water hyacinth compost (group a) than in either the ponds treated with inorganic fertilizers (group b) or the ponds where no treatment was applied (group c). the average percentages of survival in groups a, b and c were 14.3, 11.2 and 5.0, respectively. thus a ...200212365499
seasonal changes in plasma steroid levels in indian major carp labeo rohita: influence of homologous pituitary extract on steroid production and development of oocyte maturational competence.circannual variations in plasma levels of testosterone (t), 17beta-estradiol (e(2)), and 17 alpha, 20 beta-dihydroxy-4-pregnen-3-one (dhp) as well as seasonal fluctuations in ovarian steroid synthetic potential were observed in indian major carp, labeo rohita. a study was also conducted to examine the mechanism of the development of gonadotropin-induced maturational competence in oocytes of this fish. the present study recorded the lowest values of plasma e(2) and t in l. rohita during the perio ...200212392685
high vitamin a content in some small indigenous fish species in bangladesh: perspectives for food-based strategies to reduce vitamin a deficiency.recognising the importance of fish in the bangladeshi diet, the objective of the present study was to screen commonly consumed fish species for vitamin a content to evaluate the potential of fish as a vitamin a source in food-based strategies to combat vitamin a deficiency. samples of 26 commonly consumed fish species and one crustacean were collected in kishoreganj and mymensingh, bangladesh. to obtain edible parts, the fish were cleaned by bangladeshi women according to traditional practices. ...200212396468
bath immunisation of spawn, fry and fingerlings of indian major carps using a particulate bacterial antigen.larval mortality in indian major carps is one of the major problems encountered in the pond culture system. the present investigation was carried out to investigate the proper age, duration of exposure, and optimum bacterin concentration for vaccinating rohu (labeo rohita) and catla (catla catla) at their early stages with a formalin killed edwardsiella tarda bacterin suspension. the development of immunological competence was recorded with spawn of rohu and catla of 3 weeks of age exposed to a ...200212400863
potential of amaranthus seeds in supplementary feed and its impact on growth in some carps.amaranthus seeds were used at three different levels (20%, 35%, 50%) in fish diets under a semi-intensive fish culture system and their impact on the growth of common carp, cyprinus carpio, and rohu, labeo rohita, was studied. growth in terms of body weight gain was maximum in fish fed on diets containing 20% amaranthus seeds, that replaced rice bran and groundnut oil cake in the feed. overall, the fish fed on diets containing amaranthus seeds at different levels showed better growth than the co ...200312421004
utilization of fermented silkworm pupae silage in feed for carps.fermented silkworm pupae (swp) silage or untreated fresh swp pastes were incorporated in carp feed formulations replacing fishmeal. the feed formulations were isonitrogenous (30.2-30.9% protein) and isocaloric (me = 2905-2935 kcal/kg). feeding under a polyculture system consisting of 30% each of catla (catla catla), mrigal (cirrhinus mrigala) and rohu (labeo rohita) with 10% silver carps (hypophthalmychthys molitrix) was carried out in ponds to evaluate the nutritive quality of swp silage. survi ...200312421005
chromium induced alterations in some biochemical profiles of the indian major carp, labeo rohita (hamilton). 200312478433
toxicity of cypermethrin in labeo rohita fingerlings: biochemical, enzymatic and haematological consequences.the effect of exposure to sub-lethal concentrations of cypermethrin, a synthetic pyrethroid pesticide, on biochemical parameters of muscle, blood and enzyme activities in brain, liver and kidney of the indian major carp, labeo rohita was studied. the sub-lethal exposure studies were done for up to 45 days at 1/10 and 1/50 of 96 h lc(50) of cypermethrin. the 96 h lc(50) was found to be 0.139 ppm. rna levels decreased while dna levels were elevated. acid phosphatase was unchanged while alkaline ph ...200312524023
effect of formulated diet on digestive enzymes of labeo rohita (ham.).six sets of feeding experiments were carried out using formulated diets containing prawn head waste (pw), chicken intestine waste (cw), banana flower (bf), cauliflower waste (cau) dolicos lab lab (dll) and groundnut leaf (gnl) in four levels of inclusion (15, 30, 45 and 60%) to assess the pattern of distribution and activities of digestive enzymes like cellulase, amylase, maltase, invertase, protease and lipase in the digestive tracts of labeo rohita fingerlings. a control group of fish was fed ...200212561975
feed utilisation and growth in juveniles of labeo rohita under the stress of endosulfan.the objective of the present study is to evaluate the nutrition and growth of labeo rohita under the sublethal stress of endosulfan. it has been observed that the feed utilisation and growth were inferior in the fish under the stress of the toxicant. it was more so in the test fish under the influence of higher sublethal concentration. the food conversion ratio was higher suggesting that the nutrients are wasted.200212597568
immunomodulation by dietary vitamin c in healthy and aflatoxin b1-induced immunocompromised rohu (labeo rohita).the aim of this study was to examine the immunomodulatory effect of high levels of dietary vitamin c in healthy and immunocompromised rohu (labeo rohita) treated with aflatoxin b1 (afb1). four groups of rohu were fed experimental diets containing either no vitamin c or supplemented with vitamin c at 500 ppm for 60 days. on the first day of feeding, one group fed the high vitamin c diet and one fed the vitamin c deficient diet, were injected intraperitoneally with a single doses of afb1 at 1.25 m ...200312602688
effect of mercury and methyl parathion on the ovaries of labeo rohita (ham).adult pre-spawning fish labeo rohita were sublethally (1/5th 96h lc50) exposed to mercuric chloride and metacid-50 (methyl parathion). accumulation of mercury and methyl parathion was studied and it was found that pre-spawning ovary appears as a potent organ for deposition of both the pollutants. rna/dna ratio of the control and treated fish were studied. it was found that the signifcant decrease in rna/dna ratio occurs after 9 and 30 days of exposure for mercury and 30 days for methyl parathion ...200212617319
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