| verrucosispora qiuiae sp. nov., isolated from mangrove swamp, and emended description of the genus verrucosispora. | a micromonspora-like strain rtiii47(t) was isolated from mangrove swamp in sanya, hainan province, china. phylogenetic analysis based on the 16s rrna gene indicated that the strain had a close association with the genus verrucosispora and shared the highest sequence similarity to verrucosispora lutea yim 013(t) (98.0 %). the strain also showed high 16s rrna gene sequence similarities to micromonospora olivasterospora dsm 43868(t) (97.9 %), plantactinospora mayteni yim 61359(t) (97.9 %), salinisp ... | 2011 | 21873512 |
| selective quantification by 2d hsqc0 spectroscopy of thiocoraline in an extract from a sponge-derived verrucosispora sp. | we recently developed a 2d 1h-13c hsqc0 approach to quantify individual chemicals in complex mixtures. the hsqc0 approach has been implemented in phase-cycled and gradient-selective versions. as in quantitative 1d nmr, the normalized integrated signal intensities in hsqc0 are proportional to the concentrations of individual chemicals in the mixture. we applied the hsqc0 approach to selectively quantify thiocoraline present at a level of 1% w/w in an extract from a verrucosispora sp. isolated fro ... | 2011 | 21923106 |
| Verrucosispora maris sp. nov., a novel deep-sea actinomycete isolated from a marine sediment which produces abyssomicins. | Verrucosispora isolate AB-18-032(T), the abyssomicin- and proximicin-producing actinomycete, has chemotaxonomic and morphological properties consistent with its classification in the genus Verrucosispora. The organism formed a distinct phyletic line in the Verrucosispora 16S rRNA gene tree sharing similarities of 99.7%, 98.7% and 98.9% with Verrucosispora gifhornensis DSM 44337(T), Verrucosispora lutea YIM 013(T) and Verrucosispora sediminis MS 426(T), respectively. It was readily distinguished ... | 2012 | 21989685 |
| first natural analogs of the cytotoxic thiodepsipeptide thiocoraline a from a marine verrucosispora sp. | a marine verrucosispora sp. isolated from the sponge chondrilla caribensis f. caribensis was found to produce thiocoraline, a potent cytotoxic compound. five new analogs of thiocoraline were isolated and represent the first analogs of thiocoraline. 22'-deoxythiocoraline (2), thiochondrilline c (5), and 12'-sulfoxythiocoraline (6) demonstrated significant cytotoxicity against the a549 human cancer cell line with ec(50) values of 0.13, 2.86, and 1.26 ++m, respectively. the analogs provide insight ... | 2011 | 21736356 |
| genome sequence of the abyssomicin and proximicin-producing marine actinomycete verrucosispora maris ab-18-032. | verrucosispora maris ab-18-032 is a marine actinomycete that produces atrop-abyssomicin c and proximicin a, both of which have novel structures and modes of action. in order to understand the biosynthesis of these compounds, to identify further biosynthetic potential and to facilitate rational improvement of secondary metabolite titers, we have sequenced the complete 6.7 mb genome of verrucosispora maris ab-18-032. | 2011 | 21551311 |
| genus-specific primers targeting the 16s rrna gene for pcr detection of members of the genus verrucosispora. | little is known about the genus verrucosispora though it does contain organisms which produce novel antibiotics. a set of genus-specific oligonucleotide primers was generated to gain an insight into the presence, distribution and taxonomic diversity of members of this genus in diverse samples taken from marine habitats. in silico and pure culture studies showed that the primers matched perfectly with target sequences of the 16s rrna genes of representatives of the genus verrucosispora. the prime ... | 2011 | 21374042 |
| a guide to successful bioprospecting: informed by actinobacterial systematics. | new structurally diverse natural products are discovered when novel screening procedures are introduced or when high quality biological materials from new sources are examined in existing screens, hence it is important to foster these two aspects of novelty in drug discovery programmes. amongst prokaryotes, actinomycetes, notably streptomycetes, remain a rich source of new natural products though it has become increasingly difficult to find such metabolites from common actinomycetes as screening ... | 2010 | 20582471 |
| terpenoids produced by actinomycetes: isolation, structural elucidation and biosynthesis of new diterpenes, gifhornenolones a and b from verrucosispora gifhornensis ym28-088. | new terpenoids named gifhornenolones a (1) and b (2) were isolated from the culture broth of verrucosispora gifhornensis ym28-088, and their structures were established as hydroxylated isopimaradiene derivatives on the basis of extensive nmr and ms spectral analyses. in addition, a known sesquiterpene compound cyperusol c (3) was isolated. the absolute configuration of 1 was determined by nuclear overhauser effect spectroscopy (noesy) and cd spectra as 4r, 5s, 9r, 10s, 13r, and that of 2 was det ... | 2010 | 20379218 |
| verrucosispora sediminis sp. nov., a cyclodipeptide-producing actinomycete from deep-sea sediment. | an actinomycete, designated ms426t, the culture broth of which showed potent antimicrobial activity, was isolated from a deep-sea sediment sample of the south china sea. an almost-complete sequence of the 16s rrna gene of strain ms426t was determined and aligned with those of representatives of the family micromonosporaceae available in public databases. phylogenetic trees were inferred by using three algorithms. strain ms426t formed a branch adjacent to verrucosispora lutea yim 013t in a distin ... | 2010 | 19767367 |
| verrucosispora lutea sp. nov., isolated from a mangrove sediment sample. | a novel gram-positive, aerobic, spore-forming actinobacterium, designated strain yim 013t, was isolated from a mangrove sediment sample and subjected to a polyphasic taxonomic study. the peptidoglycan type was a1gamma and the major whole-cell sugars contained glucose and xylose. the phospholipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides and some unknown phospholipids. the predominant menaquinone was mk-9(h4). the major fatty aci ... | 2009 | 19620381 |
| actinobacterial diversity from marine sediments collected in mexico. | seventeen different media known to support the growth and isolation of members of the class actinobacteria were evaluated as selective isolation media for the recovery of this microbial group from marine sediments samples collected in the gulf of california and the gulf of mexico. a general selective isolation procedure was employed for six sediments and nearly 300 actinomycetes were recovered from the selective isolation plates. full 16s rrna gene sequencing revealed that the isolates belonged ... | 2009 | 19023674 |
| proximicin a, b and c, novel aminofuran antibiotic and anticancer compounds isolated from marine strains of the actinomycete verrucosispora. | a family of three novel aminofuran antibiotics named as proximicins was isolated from the marine verrucosispora strain mg-37. proximicin a was detected in parallel in the marine abyssomicin producer "verrucosispora maris" ab-18-032. the characteristic structural element of proximicins is 4-amino-furan-2-carboxylic acid, a hitherto unknown gamma-amino acid. proximicins show a weak antibacterial activity but a strong cytostatic effect to various human tumor cell lines. | 2008 | 18503194 |
| proximicins a, b, and c-antitumor furan analogues of netropsin from the marine actinomycete verrucosispora induce upregulation of p53 and the cyclin kinase inhibitor p21. | | 2008 | 18348121 |
| abyssomicins g and h and atrop-abyssomicin c from the marine verrucosispora strain ab-18-032. | abyssomicin c is a complex polyketide-type antibiotic and the first natural inhibitor of the p-aminobenzoate biosynthesis produced by the marine verrucosispora strain ab-18-032. we have now isolated three novel naturally produced abyssomicins, among them the even more active atrop-abyssomicin c. the chemical structures were elucidated by mass spectrometry and nmr spectroscopy. | 2007 | 17617698 |
| diversity of culturable actinobacteria isolated from marine sponge haliclona sp. | this study describes actinobacteria isolated from the marine sponge haliclona sp. collected in shallow water of the south china sea. a total of 54 actinobacteria were isolated using media selective for actinobacteria. species diversity and natural product diversity of isolates from marine sponge haliclona sp. were analysed. twenty-four isolates were selected on the basis of their morphology on different media and assigned to the phylum actinobacteria by a combination of 16s rrna gene based restr ... | 2007 | 17566868 |
| marine actinomycetes as a source of novel secondary metabolites. | a set of 600 actinomycetes strains which were isolated from marine sediments from various sites in the pacific and atlantic oceans were screened for the production of bioactive secondary metabolites. marine streptomycete strains were found to be producers of well known chemically diverse antibiotics isolated from terrestrial streptomycetes, as in the case of marine micromonospora strains. new marine members of the rare genus verrucosispora seem to be a promising source for novel bioactive second ... | 2005 | 15726289 |
| abyssomicins, inhibitors of the para-aminobenzoic acid pathway produced by the marine verrucosispora strain ab-18-032. | a screening method was established to detect inhibitors of the biosynthetic pathways of aromatic amino acids and para-aminobenzoic acid, the precursor of folic acid, using an agar plate diffusion assay modified as an antagonism test. by this screening method, a family of three novel polycyclic polyketides named as abyssomicins was isolated from a marine strain of verrucosispora. the main component abyssomicin c inhibits the pathway between chorismate and para-aminobenzoic acid and is strongly ac ... | 2004 | 15217192 |
| abyssomicin c-a polycyclic antibiotic from a marine verrucosispora strain as an inhibitor of the p-aminobenzoic acid/tetrahydrofolate biosynthesis pathway. | | 2004 | 15127456 |
| investigations on rhizoplane actinobacteria communities of papyrus (cyperus papyrus) from an egyptian wetland. | wetlands have important global ecological functions, which include carbon storage and water interception. wetland contributes to the maintenance of regional and global biodiversity. though many important wetland ecological functions are based on microbial metabolism, we have scanty knowledge on microbial diversity in wetlands. plant rhizoplane habitats are considered to harbor highly diverse bacterial communities. most of the floating mats on river nile are dominated by papyrus (cyperus papyrus) ... | 2002 | 12512252 |
| proposal to transfer catellatospora ferruginea and 'catellatospora ishikariense' to asanoa gen. nov. as asanoa ferruginea comb. nov. and asanoa ishikariensis sp. nov., with emended description of the genus catellatospora. | the taxonomic position of catellatospora ferruginea and 'catellatospora ishikariense' was investigated by phylogenetic, chemotaxonomic and physiological characterization. the 16s rdna sequences of the organisms were compared with those of members of the genus catellatospora and other genera of the micromonosporaceae and phylogenetic trees were inferred by using distance-matrix and parsimony methods. the organisms formed a distinct cluster within the radiation of this family that was supported by ... | 2002 | 12054264 |
| verrucosispora gifhornensis gen. nov., sp. nov., a new member of the actinobacterial family micromonosporaceae. | a gram-positive, aerobic, spore-forming actinomycete strain, hr1-2t, was isolated from a peat bog near gifhorn, lower saxony, germany. comparative analysis of the 16s rdna sequence indicated that hr1-2t was phylogenetically related to members of the family micromonosporaceae, branching adjacent to spirilliplanes yamanashiensis, couchioplanes caeruleus, catenuloplanes japonicus and members of the genus micromonospora. the affiliation to the family was supported by the presence of family-specific ... | 1998 | 9828414 |