| characterization of a c21 neutral steroid hormone transforming enzyme, 21-dehydroxylase, in crude cell extracts of eubacterium lentum. | a strain of the obligate anaerobe, eubacterium lentum, isolated from human feces, catalyzes the 21-dehydroxylation of 11-deoxycorticosterone to progesterone. a quantitative radiochromatographic assay was developed to measure 21-dehydroxylase activity in cell extracts. maximum enzyme activity in cell extracts required both a reduced pyridine nucleotide and an oxidized flavin coenzyme. however, photochemically reduced flavin (fmnh2) could replace the requirement for nad(p)h plus oxidized flavin. n ... | 1979 | 38850 |
| bile salt 3 alpha- and 12 alpha-hydroxysteroid dehydrogenases from eubacterium lentum and related organisms. | thirty-two strains of eubacterium lentum and phenotypically similar anaerobic gram-positive bacilli were screened for intracellular bile salt 3alpha- and 12alpha-hydroxysteroid dehydrogenase (hsdhase) activities. these organisms were categorized into four groups: (a) those containing 12alpha-hsdhase only (10 strains), (b) those containing 3alpha- and 12alpha-hsdhase (13 strains), (c) those containing 3alpha-hsdhase only (2 strains), and (d) those devoid of any measurable hsdhase activity (7 stra ... | 1979 | 39496 |
| [clinical experience of cefoxitin in the field of obstetrics and gynecology (author's transl)]. | cefoxitin was given to the 7 patients of infections in the field of obstetrics and gynecology, and the following results were obtained: 1) the clinical response was excellent in 2 patients, good in 4 and poor in 1 patient with the efficacy rate of 85.7%. out of the 4 patients resistant to the previous therapy with other antibiotics, 3 patients responded to cefoxitin, and all the 3 patients of anaerobic infections responded satisfactorily to cefoxitin. 2) microorganisms isolated were 2 strains ea ... | 1979 | 160956 |
| cytochrome spectrum of an obligate anaerobe, eubacterium lentum. | an obligately anaerobic bacterium, eubacterium lentum, was shown to contain cytochromes a, b, and c and a carbon monoxide-binding pigment. extracts of cells grown with hemin gave a typical absorption spectrum for cytochrome c with maxima at 424, 525, and 553 nm. extracts from cells grown in the absence of hemin also had an absorption peak corresponding to cytochrome b (562 nm) in their reduced versus oxidized spectrum. extraction of hemes and formation of pyridine hemochromes allowed quantitatio ... | 1976 | 176143 |
| arginine, a growth-limiting factor for eubacterium lentum. | eubacterium lentum is a gram-positive, asaccharolytic, obligately anaerobic bacillus, which grows to a low turbidity (absorbancy at 650 nm = 0.05 to 0.1) in peptone-based medium. the addition of substrate amounts of arginine or citrulline dramatically increased growth (absorbancy at 650 nm =1.4). the presence of an arginine dihydrolase pathway was confirmed by measurement of the necessary enzymes and demonstration of the intermediate compounds. the production of adenosine 5'-triphosphate from th ... | 1976 | 182668 |
| nad-dependent 3alpha- and 12alpha-hydroxysteroid dehydrogenase activities from eubacterium lentum atcc no. 25559. | eubacterium lentum (atcc no. 25559) was shown to contain 3alpha-and 12alpha-hydroxysteroid dehydrogenases both of which were nad-dependent and active against conjugated and unconjugated bile salts. in addition, the 3alpha-hydroxysteroid dehydrogenase was active against members of the androstan series containing a 3alpha-hydroxyl group regardless of the stereo-orientation of the 5-h-. no measurable activity against 7alpha-, 7beta-, 11beta-, or 17beta-hydroxyl groups was demonstrated. the growth o ... | 1977 | 201289 |
| isolation and characterization of human fecal bacteria capable of 21-dehydroxylating corticoids. | it has been known for a decade that human intestinal flora include organisms capable of 21-dehydroxylating corticoids. yet the identity of the organisms synthesizing 21-dehydroxylase has remained unknown. using diluted human feces, we determined the prevalence of colonies of 21-dehydroxylating organisms on a variety of media. isolation from the medium of colonies with the highest prevalence yielded an obligate anaerobe capable of 21-dehydroxylating deoxycorticosterone and tetrahydrodeoxycorticos ... | 1977 | 303887 |
| new markers for eubacterium lentum. | of 37 strains of eubacterium lentum and phenotypically similar organisms, 26 (70%) synthesized a corticoid 21-dehydroxylase and/or a 3 alpha-hydroxysteroid dehydrogenase. it appeared that the corticoid 3 alpha-hydroxysteroid dehydrogenase was identical to the bile acid 3 alpha-hydroxysteroid dehydrogenase. steroid-metabolizing enzymes were found both in e. lentum and in phenotypically similar organisms. e. lentum is characterized by nitrate reduction and enhanced growth in the presence of argini ... | 1979 | 314778 |
| antimicrobial effect of amniotic fluid. | the antimicrobial effect of amniotic fluid (af) obtained during the first (af1) and second (af2) trimesters was compared with the third (af3) against anaerobic bacteria, such as bacteroides fragilis ss. fragilis (6 strains), eubacterium lentum (3 strains), and peptostreptococcus anaerobius (4 strains). escherichia coli (5 strains) served as a positive control. af1 supported the growth of all 4 anaerobes (except b fragilis for 4 hours) for the entire 24-hour period tested. af2 supported the growt ... | 1978 | 355963 |
| immuno-electron microscopic localization of a choriogonadotropin-like antigen in cancer-associated bacteria. | the presence of choriogonadotropin-like material and its intimate association with the membranes of the wall of bacteria isolated from cancer patients, has been demonstrated by immuno-electron microscopy utilizing the indirect peroxidase-antiperoxidase-labeled antibody technique. the bacteria were an escherichia coli strain isolated from a carcinoma of colon, and atcc 25559 strain of eubacterium lentum, an anaerobic microorganism originally isolated from a rectal tumor. | 1979 | 384308 |
| bacterial metabolism of corticoids with particular reference to the 21-dehydroxylation. | clostridium paraputrificum, an obligate anaerobe recovered from human feces, reduces the alpha,beta-unsaturated carbonyl of deoxycorticosterone, corticosterone, cortisone, and cortisol. the same steroids are 21-dehydroxylated by culture 116, recently isolated from human feces, and by a closely related organism, eubacterium lentum. the 21-dehydroxylase has no effect on hydroxyl groups at carbon atoms 11 and 17. | 1979 | 429305 |
| choriogonadotropin-like antigen in an anaerobic bacterium, eubacterium lentum, isolated from a rectal tumor. | using the indirect fluorescein-labeled and indirect peroxidase-antiperoxidase-labeled immunohistochemical techniques, and utilizing both antiserum specific for the beta-subunit of choriogonadotropin and antiserum for the total hormone, we have demonstrated the presence of a choriogonadotropin-like immunoreactive material in a strain of eubacterium lenthum that was originally isolated from a rectal tumor. in contrast, both immunohistochemical reactions were negative when applied to a strain of co ... | 1979 | 468380 |
| unusual enterobacteriaceae: h2s+ shigella sonnei, one authentic and one false positive due to contamination with the obligate anaerobe eubacterium lentum. | a mixture of shigella sonnei and eubacterium lentum produced h2s in triple sugar iron agar; however, neither produced any in pure culture. a second culture of s. sonnei, isolated in japan, is thought to be the first documented h2s+ shigella. | 1976 | 767362 |
| associations between microbial species in dental root canal infections. | the existence of commensal or antagonistic relationships between microorganisms in the root canals of teeth with apical periodontitis was investigated. samples were taken from 65 infected human root canals and were analysed according to species, frequency of occurrence and proportion of the total isolated flora. the most frequent species were fusobacterium nucleatum, prevotella intermedia, peptostreptococcus micros, peptostreptococcus anaerobius, eubacterium alactolyticum, eubacterium lentum and ... | 1992 | 1494447 |
| colonisation with digoxin-reducing strains of eubacterium lentum and clostridium difficile infection in nursing home patients. | stool specimens obtained from 77 residents of a nursing home were analysed to determine the relationship between colonisation with digoxin-reducing strains of eubacterium lentum and infection with clostridium difficile. patients were categorised according to previous antibiotic treatment, prescription of enteral feedings, and pattern of bowel habits. colonisation with digoxin-reducing e. lentum was less common in subjects infected with c. difficile, in those treated with antibiotics previously, ... | 1992 | 1500644 |
| [bacteriological examination of infections in the field of oral surgery]. | etiology of bacterial infections in the field of oral surgery was studied. a total of 270 samples collected from patients with encapsulated abscess in their oral cavities was examined and bacteria were isolated from the 244 samples (90.4%). the following results were found; 1) organisms more than one from one sample were frequently isolated from cases with parodontitis, pericoronitis and gnathitis. isolation of anaerobic bacteria was common (54.2%). 2) streptococcus milleri and streptococcus san ... | 1991 | 1791332 |
| characterization of unusual tetracycline-resistant gram-positive bacteria. | tetracycline-resistant tet m-negative isolates of actinomyces viscosus, eubacterium lentum, mobiluncus curtisii, and mobiluncus mulieris were screened with the tet k, tet l, and tet o dna probes. ten (71%) of the resistant mobiluncus strains hybridized with the tet o probe, two of the three e. lentum strains hybridized with the tet k probe, and the a. viscosus isolate hybridized with the tet l probe. | 1991 | 1810204 |
| effects of subgingival irrigation on bacteremia following scaling and root planing. | the purpose of this study was to determine the effects of professional subgingival irrigation, together with subsequent patient administered home marginal irrigation, on the incidence of bacteremia after scaling and root planing (sc/rp). a total of 60 periodontal maintenance patients were assigned to either group 1: subgingival irrigation, with 0.12% chx and daily marginal irrigation with 0.04% chx; group 2: subgingival irrigation with 0.12% chx and daily marginal irrigation with water; group 3: ... | 1990 | 2201759 |
| eubacterium lentum atcc 43055, a new reference strain for quality control of anaerobic susceptibility tests. | a strain of eubacterium lentum (atcc 43055) was selected for quality control of anaerobic susceptibility tests. multilaboratory collaborative studies with 13 different antimicrobial agents were reviewed, and mic control limits were proposed for agar dilution tests with the new control strain. | 1990 | 2229368 |
| quality control criteria for testing the susceptibility of anaerobic bacteria to meropenem. | reference values for quality control of in vitro susceptibility tests with meropenem against anaerobic bacteria were determined in a multilaboratory study by the approved national committee for clinical laboratory standards agar dilution method for the four quality control strains. the study protocol also included the evaluation of microdilution testing, medium additives, and multiple lots of media. the recommended mic control ranges for three of the control organisms are as follows: bacteroides ... | 1990 | 2280013 |
| development of a differential medium for bile salt hydrolase-active lactobacillus spp. | an agar plate assay was developed to detect bile salt hydrolase activity in lactobacilli. on lactobacillus-selective mrs or rogosa sl medium supplemented with taurodeoxycholic, taurocholic, or taurochenodeoxycholic acids, bile salt hydrolysis was manifested at two intensities: (i) the formation of precipitate halos around colonies or (ii) the formation of opaque granular white colonies. sixty-six lactobacilli were tested for bile salt hydrolase activity by both the plate assay and a sensitive ra ... | 1989 | 2705765 |
| erythromycin-induced digoxin toxicity. | the potential interaction between certain antibiotics and digoxin has been discussed in the literature; however, few cases of actual erythromycin-induced digoxin toxicity have been reported. we present a case in which an 86-year-old woman who was taking digoxin 0.25 mg/d developed probably digoxin toxicity after the administration of erythromycin for the treatment of otitis media and streptococcal pharyngitis. her digoxin concentration increased from a trough of 1.9 to 5.1 nmol/l six days after ... | 1989 | 2800579 |
| fatal frontal sinusitis due to neisseria sicca and eubacterium lentum. | infectious sinusitis may on occasion be associated with meningitis, subdural empyema, epidural empyema, brain abscess, or osteomyelitis. we report a 29-year-old male patient with frontal sinusitis who developed all of these intracranial complications due to two previously unreported causative organisms, neisseria sicca and eubacterium lentum. the fulminant and fatal course resulting from locally invasive disease underscores the importance of early diagnosis and proper treatment of these complica ... | 1986 | 2873255 |
| ischaemic mouse thigh model for evaluation of pathogenicity of non-clostridial anaerobes. | a mouse thigh model has been devised in which the growing culture of non-clostridial anaerobe in the ischaemic tissue produces inflammatory swelling and death. the swelling of the right thigh served as an index of pathogenicity of the test strain in comparison to the negatively reacting left thigh which received injection of the control strain of bifidobacterium infantis. actinomyces naeslundii exceptionally caused death in all animals within 24 h. mortality and thigh swelling were pronounced (g ... | 1989 | 2914729 |
| does cytomegalovirus play a role in community-acquired pneumonia? | cytomegalovirus (cmv) is recognized as an important pathogen in the immuno-suppressed patient. sporadic case reports of cytomegalovirus community-acquired pneumonia have appeared. we studied 443 patients with community-acquired pneumonia requiring hospitalization to define the role of cytomegalovirus in this illness. four patients (0.9%) had good evidence that cytomegalovirus caused their pneumonia: 2 had the virus isolated from pulmonary tissue and 2 had cytomegalovirus inclusion bodies visuali ... | 1985 | 3000667 |
| antitumor effect of normal intestinal microflora on ehrlich ascites tumor. | in order to investigate the antitumor activity of intestinal microflora, the constitution of normal flora was examined in humans, guinea pigs and mice. it was clarified that eubacterium, bifidobacterium and bacteroides were the predominant bacterial genera in humans. in addition, neither clostridium nor enterobacteriaceae was detected in guinea pigs and neither clostridium nor bifidobacterium was present in mice. total bacterial counts in tumor-bearing mice were reduced in comparison with those ... | 1988 | 3128498 |
| antitumor activity and its properties of eubacterium lentum. | in the present study, some basic effects of eubacterium lentum (tyh-11), isolated from normal intestinal flora, upon the immune system and various experimental tumor cell lines were investigated. e. lentum showed no direct cytotoxicity against ehrlich ascites tumor, while serratia marcescens (ty-142) did show direct cytotoxicity. e. lentum presented striking antitumor activity which differed according to the injection route and time. this strain showed antitumor activity against 11 experimental ... | 1988 | 3128499 |
| bacterial metabolism of natural and synthetic sex hormones undergoing enterohepatic circulation. | steroids undergoing enterohepatic circulation are exposed to bacterial metabolism particularly by obligate anaerobes which account for 99.99% of the fecal flora. the most common transformation is hydrolysis of conjugated steroids. the glucuronidases are synthesized by escherichia coli and bacteroides species. the bacterial catabolism of unconjugated steroids may be considered under several headings: 1. reduction of ring-a due to clostridia species synthesizing specific enzymes; c. paraputrificum ... | 1987 | 3320550 |
| the synergistic effect of cefotaxime and desacetylcefotaxime against clinical isolates of anaerobic bacteria. | the synergistic interaction of cefotaxime and desacetylcefotaxime against 187 clinically significant anaerobic organisms was investigated. fusobacterium nucleatum, actinomyces odontolyticus, propionibacteria, lactobacilli, peptostreptococci, streptococcus intermedius and veillonella were sensitive to cefotaxime. both eubacterium lentum and streptococcus morbillorum were resistant. the susceptibility of the clostridia varied from 0.125 to greater than 256 mg/l; only 20% of species demonstrated sy ... | 1988 | 3396488 |
| [fundamental and clinical studies of imipenem/cilastatin sodium in the field of obstetrics and gynecology]. | fundamental and clinical studies of a new carbapenem antibiotic, imipenem/cilastatin sodium (mk-0787/mk-0791), were carried out to evaluate the drug in treating infections of the female genital organs. the following results were obtained: in 4 patients undergoing hysterectomy, the penetration of mk-0787 and mk-0791 into female genital organ tissues was studied following a 30-minute intravenous drip infusion of mk-0787/mk-0791 500 mg/500 mg. plasma levels of mk-0787 and mk-0791 in cubital venous ... | 1986 | 3463786 |
| comparative serum bactericidal activity against test anaerobes in volunteers receiving imipenem, clindamycin, latamoxef and metronidazole. | ten healthy volunteers received on separate days the following regimens: imipenem 500 mg, clindamycin 600 mg, latamoxef 1 g, and metronidazole 500 mg. the antibiotics were given intravenously as an infusion over 15 min. blood samples were obtained before and 30 min, 1 and 6 h after the start of the infusion. serum bacteriostatic and bactericidal activities were measured against the following strains of strict anaerobes: two strains of bacteroides fragilis, one strain each of b. vulgatus, b. thet ... | 1987 | 3471750 |
| reductive inactivation of digitoxin by eubacterium lentum cultures. | the obligate anaerobe eubacterium lentum inactivated the cardiac glycoside digitoxin by reducing the double bond in the lactone ring. this conversion was quantitative when the substrate was incubated at a concentration of 10 micrograms/ml. the reduction reaction coincided with the growth phase of the bacterium. the stereochemical configuration at c-20 of the reduction product dihydrodigitoxin was found to be r. incubation of digitoxigenin and its mono- and bisdigitoxosides individually with e. l ... | 1987 | 3579289 |
| systematic analysis of the long-chain components of eubacterium lentum. | the cellular long-chain component patterns of 33 strains of eubacterium lentum were determined by gas chromatography. two main types of long-chain component patterns were distinguished. the first (26 strains) was characterized by saturated branched-chain fatty acids (br14:0, br15:0, br16:0 and br17:0). the second (7 strains) did not contain branched-chain fatty acids and was characterized by saturated straight-chain fatty acids (11:0, 12:0, 14:0 and 16:0). both types contained fatty aldehydes an ... | 1987 | 3655724 |
| reduction of digoxin to 20r-dihydrodigoxin by cultures of eubacterium lentum. | the anaerobic bacterium eubacterium lentum, a common constituent of the intestinal microflora, inactivates digoxin by reducing the unsaturated lactone ring. reduction of the cardiac glycoside by growing cultures of e. lentum atcc 25559 proceeded in a stereospecific manner, with the 20r-dihydrodigoxin constituting more than 99% of the product formed. this is in contrast to the 3:1 ratio of 20r and 20s epimers formed in the chemical catalytic hydrogenation. formation of the reduced glycosides proc ... | 1986 | 3729400 |
| comparative in vitro activity of cefbuperazone against anaerobic bacteria isolated from community hospitals. | the activity of cefbuperazone against 266 strains of anaerobic bacteria was determined by the agar dilution method and compared with cefoxitin, moxalactam, piperacillin, and clindamycin. all strains were recent clinical isolates from community hospitals. all agents tested showed good activity against bacteroides fragilis, fusobacterium spp., propionibacterium spp., clostridium septicum, clostridium perfringens, and the anaerobic, gram-positive cocci and gram-negative cocci. cefbuperazone, cefoxi ... | 1985 | 3985600 |
| anaerobic bacteria in urine before and after prostatic massage of infertile men. | in the present study 25 infertile men delivered urine before and after prostatic massage. expressed prostatic secretion (eps) was obtained from 11 men. aerobic and anaerobic bacterial analyses showed that a total of 33 isolates was found in samples of urine voided before massage as compared to 59 isolates after massage of the prostate. there was an increase in the number of anaerobes whereas there was no change in the number of aerobes. the occurrence of eps did not influence the number of aerob ... | 1985 | 4000063 |
| 12 beta-dehydrogenation of bile acids by clostridium paraputrificum, c. tertium, and c. difficile and epimerization at carbon-12 of deoxycholic acid by cocultivation with 12 alpha-dehydrogenating eubacterium lentum. | 12 beta-hydroxysteroid dehydrogenating activities were detected in 13 strains of clostridium paraputrificum, 1 strain of c. tertium, and 1 strain of c. difficile, together with a 3 alpha- and 3 beta-hydroxysteroid dehydrogenase system in many strains. redox reactions a c-12 of disubstituted and trisubstituted bile acids were performed unspecifically by representative strains of c. paraputrificum. 3 alpha,12 beta-, 3 beta,12 beta-dihydroxy-, 3 alpha, 7 alpha, 12 beta-trihydroxy-, and 3-keto,12 be ... | 1985 | 4004226 |
| isolation and characterization of a novel vitamin-k from eubacterium lentum. | a novel fat-soluble vitamin k like molecule was isolated from the prokaryote, eubacterium lentum, and its structure investigated by mass spectrometry and proton nuclear magnetic resonance spectrometry. on the basis of these studies the novel quinone is shown to be 2,5 and 6- or 2,7 and 8-trimethyl-3-farnesylfarnesyl-1,4-naphthoquinone. | 1985 | 4074371 |
| [antibacterial activity of mt-141 against anaerobic bacteria]. | in vitro and in vivo antibacterial activities of mt-141, a new cephamycin, against anaerobic bacteria were compared with those of cefmetazole (cmz), cefoxitin, cefotaxime, ceftizoxime, latamoxef and cefazolin to obtain the following results. mt-141 showed high activity against a wide variety of anaerobic bacteria including b. fragilis and c. difficile except for eubacterium lentum, e. aerofaciens and b. furcosus. antibacterial activity of mt-141 against anaerobic bacteria was almost independent ... | 1984 | 6092738 |
| comparison of moxalactam (ly127935) and cefotaxime against anaerobic bacteria. | the in vitro activities of moxalactam (ly127935 [6059s]) and cefotaxime were compared with those of cefoxitin, cefamandole, cefuroxime, carbenicillin, and penicillin by agar dilution susceptibility testing of a variety of anaerobic bacteria. moxalactam proved to be the most active agent tested against bacteroides fragilis and other species of the b. fragilis group. moxalactam and cefotaxime showed activity similar to the other drugs against the remaining species of bacteroides, fusobacterium, ac ... | 1980 | 6249198 |
| the survival of anaerobic bacteria at 4 degrees c and 22 degrees c on swabs in three transport systems. statistical evaluation by application of a variance component model. | the survival of anaerobic bacteria on swabs in three transport systems at 4 degrees c and 22 degrees c was evaluated. the transport systems were a charcoal-impregnated cotton swab in modified stuart's transport medium (ms), a plain cotton swab in a dry aerobic tube (da) and a plain cotton swab in an aerobic agar tube (aa). the test strains were bacteroides fragilis, b. melaninogenicus ss. intermedius, fusobacterium necrophorum, peptostreptococcus anaerobius, eubacterium lentum and clostridium ra ... | 1983 | 6346789 |
| the recovery of anaerobic bacteria from swabs in three transport systems. statistical evaluation by application of a variance component model. | the recovery of six anaerobic species from swabs in three transport systems was quantitatively evaluated. the test strains were bacteroides fragilis, b. melaninogenicus ss. intermedius, fusobacterium necrophorum, peptostreptococcus anaerobius, eubacterium lentum, and clostridium ramosum. the transport systems were a charcoal-impregnated cotton swab in modified stuart's transport medium (ms), or a plain cotton swab in either a dry aerobic tube (da) or an aerobic agar tube (aa). swabs were charged ... | 1983 | 6346790 |
| reduction of 17-keto steroids by anaerobic microorganisms isolated from human fecal flora. | the 17-keto function of phenolic steroids is reduced by the following intestinal bacteria: eubacterium lentum, clostridium paraputrificum, clostridium j-1 and clostridium innocuum. the 17-keto group of androstenedione is reduced solely by bacteroides fragilis. | 1984 | 6477942 |
| comparative efficacy of four antibiotics in anaerobic pulmonary infection. an experimental model in rabbits. | the efficacy of cefoxitin, mezlocillin, latamoxef and metronidazole in anaerobic lung infection was studied using a rabbit model. a mixture of bacteroides fragilis, peptococcus morbillorum, eubacterium lentum and fusobacterium nucleatum was inoculated transtracheally to produce infection within the lung. mezlocillin was most effective, achieving bacteriologic cure in 5 out of 8 animals. with cefoxitin therapy, 4 out of 8 became bacteriologically sterile. severe diarrhea with elevated titers of c ... | 1984 | 6488936 |
| biotransformation of linoleic acid and bile acids by eubacterium lentum. | eubacterium lentum is a gram-positive, nonsporeforming, nonmotile, asaccharolytic anaerobe. in the present investigations, 3 e. lentum strains (group e) isolated from rat feces were compared with 30 e. lentum strains (groups a, b, c, and d) previously studied by macdonald et al. (i. a. macdonald, j. f. jellet, d. e. mahony, and l. v. holdeman, appl. environ. microbiol. 37:992-1000, 1979). all strains alkalized (ph 8 to 8.5) arginine-containing (2 to 15 mg/ml) culture media, and growth of the maj ... | 1984 | 6582800 |
| fecal steroid 21-dehydroxylase, a potential marker for colorectal cancer. | eubacterium lentum and phenotypically similar organisms synthesize a steroid 21-dehydroxylase which converts biliary tetrahydrodeoxycorticosterone to pregnanolone. tetrahydrodeoxycorticosterone, in contrast to pregnanolone, is carcinogenic for hamster embryonic cells (hect test). in patients with recently diagnosed, untreated sigmoidal or rectal cancer the fecal concentration of 21-dehydroxylating organisms is reduced by more than 99% as compared with age-matched controls. the lack of fecal 21-d ... | 1983 | 6603789 |
| the effect of lincomycin-neomycin treatment on experimental anaerobic bacterial bovine mastitis. | three healthy lactating quarters of a friesland cow were each experimentally infected with a pure culture of a strain of either bacteroides fragilis, eubacterium lentum or a peptostreptococcus sp. respectively. the onset and progression to clinical mastitis was monitored 12 hourly by examination for clinical signs of inflammation, bacterial culture, somatic cell counts and with a strip cup. all infected quarters developed clinical mastitis within 24 hours. the 2 quarters infected with b. fragili ... | 1983 | 6668573 |
| 7 alpha-dehydroxylation of bile acids by resting cells of a eubacterium lentum-like intestinal anaerobe, strain c-25. | 7 alpha-dehydroxylation of cholic acid and chenodeoxycholic acid by whole cells of strain c-25, a eubacterium lentum-like intestinal anaerobe, was studied. 7 alpha-dehydroxylase activity was observed only in whole cells grown in the presence of the primary bile acid (cholic acid or chenodeoxycholic acid). chenodeoxycholic acid was twice as effective as cholic acid as an inducer. although cells grown in the presence of chenodeoxycholic acid had no significant substrate specificity for the two pri ... | 1984 | 6721490 |
| cooperative formation of omega-muricholic acid by intestinal microorganisms. | three anaerobic bacteria, isolated from the ceca of rats and mice, converted, through a concerted mechanism, beta-muricholic acid, the predominant bile acid in germfree rats, into omega-muricholic acid. one isolate was a eubacterium lentum strain; the second and third isolates were tentatively identified as atypical fusobacterium sp. strains. the conversion of beta-muricholic acid into omega-muricholic acid proceeded in two steps: e. lentum oxidized the 6 beta-hydroxyl group of beta-muricholic a ... | 1983 | 6824314 |
| enhancement of the 7 alpha-dehydroxylase activity of a gram-positive intestinal anaerobe by flavins. | the addition of flavins to the growth medium specifically enhanced the 7alpha-dehydroxylation of bile acids by anaerobically growing cultures of a eubacterium lentum-like intestinal anaerobe, strain c-25, without an increase in cell growth. the order of the enhancement of the reaction was flavin adenine dinucleotide > flavin mononucleotide >> riboflavin. | 1983 | 6824319 |
| digoxin-inactivating bacteria: identification in human gut flora. | digoxin, the most widely used cardiac glycoside, undergoes significant metabolic conversion in many patients to cardioinactive metabolites in which the lactone ring is reduced. this appears to occur within the gastrointestinal tract. an attempt was made to isolate and identify the organisms capable of reducing digoxin from stool cultures obtained from human volunteers. of hundreds of isolates studied, only eubacterium lentum, a common anaerobe of the human colonic flora, converted digoxin to red ... | 1983 | 6836275 |
| penicillin failure in the treatment of bacteroides fragilis lung abscess. experimental study in rabbits. | carbenicillin, chloramphenicol, doxycycline, and clindamycin were compared with penicillin for the treatment of lung abscess in an animal model produced by transtracheal inoculation of a mixture of anaerobes: bacteroides fragilis, peptococcus morbillorum, eubacterium lentum and fusobacterium nucleatum. both chloramphenicol and doxycycline eliminated the bacteria but failed to close the abscess cavity. carbenicillin, although it eradicated b. fragilis, failed to close the abscess cavity in 3 of 6 ... | 1983 | 6872619 |
| transformation of bile acids by eubacterium lentum. | a group of fecal isolates identified as eubacterium lentum elaborated 3 alpha-, 7 alpha-, and 12 alpha-dehydrogenases and also an epimerizing enzyme(s) for the 3 alpha-hydroxy group. the activities of the enzymes, however, were variably manifested according to the kind of bile acid substrate and the oxygen tension under which the reaction occurred. | 1981 | 6947718 |
| enhancement of the 7 alpha-dehydroxylase activity of a gram-positive intestinal anaerobe by bacteroides and its significance in the 7-dehydroxylation of ursodeoxycholic acid. | the 7 alpha-dehydroxylation of chenodeoxycholic acid (cdca) and cholic acid (ca) by a eubacterium lentum-like intestinal anaerobe was specifically enhanced by the bacteroides present in mixed cultures and also by the addition to the growth medium of cell extracts from the bacteroides. the 7 alpha-dehydroxylating organism also possessed 7 alpha-hydroxysteroid dehydrogenase activity, and, in collaboration with a 7 beta-dehydrogenating organism, converted ursodeoxycholic acid (udca) into cdca. larg ... | 1982 | 6960114 |
| characterization of a corticosteroid 21-dehydroxylase from the intestinal anaerobic bacterium, eubacterium lentum. | an oxygen-sensitive corticosteroid 21-dehydroxylase has been characterized in cell extracts of eubacterium lentum. the enzyme was highly specific for corticosteroids containing and alpha-ketol structure and required fmnh2 or reduced benzyl viologen for activity. the enzyme used deoxycorticosterone, deoxycortisol, dehydrocorticosterone, and corticosterone as substrates. substrate saturation kinetics using [3h]corticosterone yielded an apparent km of 7.35 microm and a vmax of 15.4 nmol (11 beta-[3 ... | 1980 | 6967506 |
| spurious hydrogen sulfide production by providencia and escherichia coli species. | hydrogen sulfide production was noted in two escherichia coli strands and one provaidenica alcalifaciens (proteus inconstans a) strain isolated from clinical stool specimens durin the summer of 1979. an investigation into this phenomenon revealed the predence of eubacterium lentum, an anaerobe, growing in synergism with the enterobacteriaceae and producing h2s. the implications of this association are discssed with reference to clinical microbiology laboratory practice. | 1980 | 7000823 |
| chemoprophylaxis of anaerobic pulmonary infections. an experimental study in rabbits. | chemoprophylaxis of anaerobic pulmonary infection due to aspiration was studied in a rabbit model with the view of comparing the efficacy of procaine penicillin, clindamycin, chloramphenicol, doxycycline and cefoxitin. the antibiotic treatment was commenced immediately following transtracheal inoculation of a mixture of bacteroides fragilis, streptococcus morbillorum, fusobacterium nucleatum and eubacterium lentum. treatment was stopped after 48 h and lungs were examined for evidence of infectio ... | 1982 | 7075325 |
| 16 alpha-dehydration of corticoids by bacteria isolated from rat fecal flora. | two strains (no. 144 and no. 146) of rat intestinal anaerobic bacteria, phenotypically similar to eubacterium lentum, were isolated and found capable of 16 alpha-dehydrating corticoids. the initial step in the 16 alpha-dehydration of 16 alpha-hydroxyprogesterone was dehydration at the c-16 and c-17 position with the accumulation of 16-dehydroprogesterone. this step required the side chain at c-17. in bacterial cultures the 16-dehydroprogesterone was then slowly reduced to iso-progesterone. 16 al ... | 1982 | 7078162 |
| biotransformation of 1-nitropyrene in intestinal anaerobic bacteria. | mutagenic nitroaromatic compounds have recently been found in photocopies, urban atmosphere, automobile exhaust and wastewater. 1-nitropyrene (1-np) is readily formed when pyrene, ubiquitous in the environment, is exposed to nitrogen dioxide in the urban atmosphere or in automobile exhaust, and is highly mutagenic, inducing 449 his+ revertants/plate/nmol from salmonella typhimurium strain ta98 in the absence of s9 fraction in the salmonella-microsome test. it is possible to swallow sputum or som ... | 1982 | 7167066 |
| formation of 20 beta-dihydrosteroids by anaerobic bacteria. | cortisol was metabolized to a variety of products, among them small amounts of cortol by fecal flora of humans and rats. a microorganism, bifidobacterium adolescentis, isolated from both sources, synthesized a 20-hydroxysteroid dehydrogenase which reduced cortisol to 20 beta-dihydrocortisol. the metabolite was reduced to cortol by clostridium paraputrificum. the 20-hydroxysteroid dehydrogenase showed a wide substrate specificity; it was independent of the 4-ene and the configuration at c3, c-11, ... | 1982 | 7176656 |
| inactivation of digoxin by eubacterium lentum, an anaerobe of the human gut flora. | digoxin is converted to cardioinactive reduced metabolites (drp) in vivo and in vitro by the human gut flora. digoxin inactivation is mediated by eubacterium lentum, a normal inhabitant of the gastrointestinal flora. e. lentum appears to be the sole organism performing this reaction. fecal bacteria that convert digoxin to drp in vitro have been found to be present in some subjects who fail to make drp in vivo. drp-forming e. lentum have been isolated from the stools of two such subjects. when in ... | 1982 | 7182977 |
| animal model for anaerobic lung abscess. | there are no satisfactory animal models for the study of anaerobic lung abscess. aspiration of food, gastric mucin, or hydrochloric acid, or any combination of these, along with oropharyngeal bacteria, is commonly believed to cause aspiration pneumonia and lung abscess. in the animal model described, none of the adjuvants was effective in producing anaerobic lung abscesses. anaerobic bacteria derived from dental scrapings of a healthy adult (peptococcus morbillorum, fusobacterium nucleatum, euba ... | 1981 | 7216463 |
| transformation of bile acids by mixed microbial cultures from human feces and bile acid transforming activities of isolated bacterial strains. | microbiol transformation of cholic acid and chenodeoxycholic acid by anaerobic mixed cultures of human fecal microorganisms was investigated, and the results were examined in relation to the bile acid transforming activities of 75 bacterial strains isolated from the same fecal cultures. the reactions involved in the mixed cultures were dehydrogenation and dehydroxylation of the 7 alpha-hydroxy group in both primary bile acids and epimerization of the 3 alpha-hydroxy group in all metabolic bile a ... | 1981 | 7253965 |
| isolation and significance of anaerobic bacteria isolated from cases of bovine mastitis. | the role of obligate anaerobic bacteria in the aetiology of mastitis of lactating dairy cows was investigated. anaerobes were isolated from 12% of lactating mastitic cows, which were representative of 50% of the 10 dairy herds examined. bacteroides fragilis was the most frequently isolated organism of (50%), followed by peptococcus indolicus (33%), eubacterium lentum (33%), e. aerofaciens (17%), propionibacterium granulosum (17%) and an anaerobic streptococcus sp. (17%). these obligate anaerobes ... | 1981 | 7312305 |
| infectious complications of cervical cerclage. | a study of 115 gravid patients who required cervical cerclage indicated that the optimum time to perform the surgical procedure was between the fourteenth and eighteenth weeks. if the operation is delayed until later in the second trimester, the incidence of chorioamnionitis is increased 2.6-fold, and premature rupture of the membranes prior to the thirty-second week of gestation is trebled. amniocentesis performed in nine patients with chorioamnionitis and intact membranes after cerclage demons ... | 1981 | 7315918 |
| isolation and characterization of fecal bacteria capable of 16 alpha-dehydroxylating corticoids. | for more than a decade it has been known that the fecal flora of humans and rats includes organisms capable of 16 alpha-dehydroxylating corticoids, but their identity has remained unknown. to isolate these organisms, mueller-hinton agar plates were seeded with fresh feces from proteus-free rats and incubated anaerobically. on an average, 1 of every 35 colonies consisted of organisms synthesizing 16 alpha-dehydroxylase. isolation of the individual colonies yielded two obligate anerobes, strains 1 ... | 1980 | 7425626 |
| effect of temperature, humidity and exposure to oxygen on the survival of anaerobic bacteria. | the relative effect of humidity, temperature and exposure to oxygen on the survival of 11 strains of bacteriodes spp., fusobacterium necrophorum, eubacterium lentum, clostridium ramosum, peptococcus asaccharolyticus and peptostreptococcus anaerobius was evaluated. suspensions of the test strains were applied to membrane filters either in empty sterile petri dishes or on plates of non-nutrient agar to provide dry or moist conditions. the filters were stored aerobically or anaerobically in an atmo ... | 1980 | 7431377 |
| effect of h2-receptor antagonists on bile acid metabolism. | several reports have been presented concerning pronounced overgrowth of bacteria in gastric juices of patients treated with h2-receptor antagonists. however, there has been no report concerning influence of h2-receptor antagonists on jejunal flora. thus, to investigate the influence and its effect on bile acid metabolism, this study was performed: 1) to examine whether patients with gastric ulcers who have been treated with h2-receptor antagonists have positive bile acid breath tests due to bact ... | 1995 | 7735920 |
| absence of cholic acid 7 alpha-dehydroxylase activity in the strains of lactobacillus and bifidobacterium. | to investigate the presence of 7 alpha-dehydroxylase activity on bile acids in the bacterial strains of fermented milk products, 46 strains of lactobacillus casei, lactobacillus paracasei, lactobacillus rhamnosus, lactobacillus acidophilus, lactobacillus gasseri, bifidobacterium adolescentis, bifidobacterium bifidum, bifidobacterium breve, bifidobacterium infantis, bifidobacterium longum, lactococcus lactis spp. lactis, and streptococcus salivarius spp. thermophilus were tested for their ability ... | 1994 | 7814703 |
| anaerobic susceptibility testing. slight differences in inoculum size can make a difference in minimum inhibitory concentrations. | in the present study, we compared the actual inoculum density from inoculated broth microdilution wells to the targeted inoculum size (10(5) cfu/well) when the inoculum was prepared using a mcfarland nephelometer to achieve the standard density. three target inoculum sizes (10(5), 5 x 10(5), and 10(6) cfu/well) were used to compare the effect of slight inoculum size increases of both atcc and clinical strains of anaerobes on mics of various antimicrobials. actual colony counts of bacteroides fra ... | 1994 | 7924212 |
| growth inhibition of clostridium difficile by intestinal flora of infant faeces in continuous flow culture. | growth of clostridium difficile was inhibited more strongly in continuous flow (cf) culture with c. difficile-negative faeces of infants than with c. difficile-positive faeces. culture of faecal flora of infants yielded a greater variety of bacterial species in c. difficile-negative than in c. difficile-positive faeces. in the mixed cf culture of c. difficile with enterococcus avium, bacteroides distasonis, eubacterium lentum, c. ramosum, c. perfringens and either escherichia coli or klebsiella ... | 1994 | 8114067 |
| [cytotoxicity of anaerobic bacteria isolated from infected root canal]. | bacteria in the infected root canal play an important role in the progression of the periapical lesion. the purpose of the present study was to investigate the cytotoxic effects of bacteria isolated from an infected root canal on the periapical lesion. four obligately anaerobic bacteria were isolated and their sonic extracts (se) were prepared. the cytotoxic assay of each se for mc3t3-e1 cell was used with respect to the morphological cell changes and [3h]-thymidine uptake. the following results ... | 1993 | 8228523 |
| uv red fluorescence of eubacterium lentum. | twenty-nine clinical isolates of eubacterium lentum and two type species were evaluated for the ability to fluoresce under uv light. twenty-one of the 29 isolates and both of the reference strains showed orange-to-red fluorescence. this fluorescence did not require blood or hemin in the culture media and did not fade upon air exposure. the fluorescent pigment, after extraction by 1 n naoh, showed peak excitation at a wavelength of around 400 nm. the capacity of e. lentum to produce fluorescence ... | 1993 | 8463378 |
| reduction of oxalate content of foods by the oxalate degrading bacterium, eubacterium lentum wyh-1. | urinary oxalate may contribute far more than urinary calcium to the pathogenesis of urinary calculi. urinary oxalate may be reduced by restricting the intake of foods high in oxalate. the oxalate content foods might be reduced by oxalate-degrading bacteria. the purpose of this experiment was to reduce the oxalate content of foods with an oxalate-degrading bacterium which was isolated from the feces of japanese male. | 1996 | 8646596 |
| antitumor mechanisms of eubacterium lentum and its components. | in the present study, some antitumor mechanisms of eubacterium lentum (tyh-11) and bacterial components having antitumor effects were investigated. e.lentum induced maximum nk cell activity in c3h/he mice on day 1 after injection (90.6% against 33.9% of control at e:t ratio 50:1) and the activity was kept at a level of 48.6% on day 7. tumoricidal peritoneal macrophages were induced 9 days after e.lentum injection into balb/c mice (56.2% against 10.1% control at e:t ratio 10:1). tumoricidal macro ... | 1995 | 8703241 |
| pyomyositis due to eubacterium lentum and streptococcus constellatus from a periodontal source. | | 1996 | 8824994 |
| alteration of bile acid metabolism by cimetidine in healthy humans. | to clarify an effect of cimetidine on bile acid metabolism, we evaluated whether an increased deconjugation of bile acids would occur in healthy humans who have received cimetidine. we examined: 1) whether healthy volunteers taking cimetidine would have positive bile acid breath tests because of bacterial overgrowth in the jejunum; 2) whether the isolated bacteria would exhibit deconjugation ability; and 3) whether a change in gastric ph was related to the bacterial overgrowth. | 1996 | 8952227 |
| protein degradation by prevotella intermedia and actinomyces meyeri supports the growth of non-protein-cleaving oral bacteria in serum. | the proteolytic activities of oral bacteria are thought to play an important role in the aetiology of dental abscesses. bacteria-derived proteases may contribute to tissue destruction, and are likely to impair host defence by degrading immunoglobulins and complement. degraded periodontal tissue and tissue fluid are likely to constitute essential sources of nutrients in the abscess. tissue fluid, which is derived from serum, is rich in protein and poor in carbohydrate, suggesting that breakdown o ... | 1997 | 9178115 |
| associations of endodontic symptoms and signs with particular combinations of specific bacteria. | significant associations have been reported between (a) specific bacterial species isolated from root canals and (b) between individual bacterial species and endodontic symptoms and signs. the prime objective of this study was to determine whether particular combinations of specific bacteria are associated with individual endodontic symptoms and signs. seventy root canals were investigated microbiologically taking care to maintain the viability of obligate anaerobes, which accounted for 64% of t ... | 1996 | 9206427 |
| clarithromycin-induced digoxin intoxication. | to report a case of clarithromycin-induced digoxin intoxication. | 1997 | 9296239 |
| nitrate-reducing bacteria in diversion colitis: a clue to inflammation? | a pathogenic role of nitric oxide has been suggested in acute and chronic intestinal inflammation. we took the opportunity offered by studies in patients with excluded colon, which represents a model of chronic intestinal inflammation with no exogenous nitrite or nitrate supply, to evaluate the quantity and the quality of nitrate reducers in diversion colitis. thirty patients (17 men, 13 women, mean age 45 years) having an excluded colon for various reasons were sampled by rectal swabs and compa ... | 1997 | 9440640 |
| evaluation of root canal bacteria and their antimicrobial susceptibility in teeth with necrotic pulp. | this study aimed to evaluate the microbiota of necrotic pulp in teeth without carious lesions where the crown and root were intact and to test the sensitivity of this microbiota to antibiotics in order to improve treatment. the necrotic pulp was sampled from 26 single-rooted teeth in intact pulp chambers. a total of 84 strains were isolated. the number of species isolated per tooth varied from 2 to 8, with a strong component (81%) of anaerobic bacteria. the most commonly represented species were ... | 1997 | 9467386 |
| cellular fatty acid composition, soluble-protein profile, and antimicrobial resistance pattern of eubacterium lentum. | phenotypic heterogeneity among isolates of eubacterium lentum has been recognized for many years. to better delineate their taxonomic relatedness, 29 clinical isolates of e. lentum were examined for soluble-protein content, cellular fatty acid profile, and antimicrobial resistance pattern in order to ascertain whether differences in these characteristics could be correlated with differences in biochemical activities. among 29 isolates we could identify 6 that were different from all the others. ... | 1998 | 9508307 |
| omeprazole induces altered bile acid metabolism. | it has been reported that the acidity of gastric contents could be an important factor in regulating jejunal flora. | 1998 | 9536953 |
| restriction fragment-length polymorphism analysis of 16s rdna from oral asaccharolytic eubacterium species amplified by polymerase chain reaction. | restriction fragment-length polymorphism (rflp) analysis of 16s rdna amplified by polymerase chain reaction was used to generate restriction profiles of the type strains of oral asaccharolytic eubacterium species, that is, eubacterium brachy, eubacterium exiguum, eubacterium lentum, eubacterium minutum, eubacterium nodatum, eubacterium saphenum, eubacterium timidum and 33 asaccharolytic eubacterium strains isolated from oral sites. the 16s rrna gene sequences from isolated genomic dna samples we ... | 1998 | 9573818 |
| experimental root canal infections in conventional and germ-free mice. | a small animal model was evaluated to study the interrelationships between microorganisms after their implantation in root canals (inferior central incisors) using germ-free (gf) and conventional (cv) mice. the selected microorganisms were: porphyromonas endodontalis (atcc 35406), eubacterium lentum (atcc 25559), peptostreptococcus anaerobius (atcc 27337), fusobacterium nucleatum (atcc 10953), escherichia coli (atcc 25922), and enterococcus faecalis (atcc 4083). only p. anaerobius, e. coli, and ... | 1998 | 9693583 |
| deconjugation ability of bacteria isolated from the jejunal fluid of patients with progressive systemic sclerosis and its gastric ph. | our goal was to demonstrate the role of bacteria in altered bile acid metabolism, which overgrow in the upper small intestine of patients with progressive systemic sclerosis. we identified the bacterial species, isolated from the jejunal fluid obtained from patients with progressive systemic sclerosis, who had previously shown an increase in 14co2, specific activity on breath test, and normal controls. after which, we investigated the deconjugation ability of the isolated bacteria and the relati ... | 1998 | 9840121 |
| clinical and microbiological characteristics of smokers with early onset periodontitis. | cigarette smoking is a potential risk factor which has recently been associated with periodontal disease progression. the objective of this study was to compare the microbial profile of smokers and non-smokers in a group of patients with early onset periodontitis. the study population consisted of 60 healthy individuals, 40 males and 20 females aged 22 to 35 yr, exhibiting early onset periodontitis. thirty patients were smokers (30.9 cigarettes/d) and 30 non-smokers. smokers had a higher proport ... | 1999 | 10086883 |
| the family coriobacteriaceae: reclassification of eubacterium exiguum (poco et al. 1996) and peptostreptococcus heliotrinreducens (lanigan 1976) as slackia exigua gen. nov., comb. nov. and slackia heliotrinireducens gen. nov., comb. nov., and eubacterium lentum (prevot 1938) as eggerthella lenta gen. nov., comb. nov. | 16s rrna gene sequences were determined for eubacterium exiguum and peptostreptococcus heliotrinreducens. these species were found to be closely related and, together with eubacterium lentum, to constitute a branch of the coriobacteriaceae. two new genera are proposed on the basis of phenotypic characteristics and 16s rrna gene sequence comparisons: slackia to include the bile-sensitive species eubacterium exiguum and p. heliotrinreducens, and eggerthella to include the bile-resistant eubacteriu ... | 1999 | 10319481 |
| cryptobacterium curtum gen. nov., sp. nov., a new genus of gram-positive anaerobic rod isolated from human oral cavities. | novel eubacterium-like isolates, strains 12-3t and kv43-b, which were isolated from the periodontal pocket of an adult patient with periodontal disease and necrotic dental pulp, respectively, were studied taxonomically and phylogenetically. the morphological and differential biochemical characteristics of these organisms are also described in this paper. these organisms were gram-positive, anaerobic, non-spore-forming, rod-shaped bacteria that were inert in most of the conventional biochemical t ... | 1999 | 10425779 |
| phylogenetic evidence for the transfer of eubacterium lentum to the genus eggerthella as eggerthella lenta gen. nov., comb. nov. | eubacterium lentum has unique phenotypic characters within the genus eubacterium. the 16s rrna sequence of eubacterium lentum was determined and its phylogenetic position was defined. this micro-organism is a member of the genus eubacterium but it is not closely related to eubacterium limosum, the type species of the genus eubacterium, and is nearer to collinsella aerofaciens and coriobacterium glomerans. a pcr-based identification system using species-specific primers designed on the basis of d ... | 1999 | 10555354 |
| description of mogibacterium pumilum gen. nov., sp. nov. and mogibacterium vescum gen. nov., sp. nov., and reclassification of eubacterium timidum (holdeman et al. 1980) as mogibacterium timidum gen. nov., comb. nov. | a new genus, mogibacterium, is proposed for anaerobic, non-spore-forming, gram-positive, rod-shaped bacteria which have been isolated from the periodontal pockets of adult human patients with periodontal disease and infected root canals. the novel isolates, strains d2-18t, ba11a-f and d5-2t, were inert in most of the conventional biochemical tests and phenotypically resemble asaccharolytic eubacterium species. the protein profiles of whole cells on sds-page gels and western immunoblotting reacti ... | 2000 | 10758875 |
| bacteriology of histopathologically defined appendicitis in children. | acute appendicitis is the most common surgical emergency in childhood. however, the pathogenesis and detailed microbiology are obscure. | 2000 | 11099090 |
| enterococcal vanb resistance locus in anaerobic bacteria in human faeces. | while developing a rapid method to detect carriers of vancomycin-resistant enterococci (vre), we found the vanb gene by pcr in 13 of 50 human faecal specimens that did not contain culturable vre. passaging under antibiotic selection allowed us to isolate two species of anaerobic bacteria that were vanb pcr positive, vancomycin resistant, and teicoplanin sensitive. sequence analysis of the 16s rrna genes showed that one isolate resembled eggerthella lenta (98% identity), and the other clostridium ... | 2001 | 11265957 |
| rapid detection of human fecal eubacterium species and related genera by nested pcr method. | pcr procedures based on 16s rdna gene sequence specific for seven eubacterium spp. and eggerthella lenta that predominate in the human intestinal tract were developed, and used for direct detection of these species in seven human feces samples. three species of eggerthella lenta, eubacterium rectale, and eubacterium eligens were detected from seven fecal samples. eubacterium biforme was detected from six samples. it was reported that e. rectale, e. eligens, and e. biforme were difficult to detec ... | 2001 | 11386422 |
| association of early onset periodontitis microbiota with aspartate aminotransferase activity in gingival crevicular fluid. | the objective of this study was to determine the relationship between the activity of the enzyme aspartate aminotransferase (ast) in gingival crevicular fluid (gcf) using the colorimetric periogard (ptm) test and the subgingival microflora in early onset periodontitis lesions. | 2001 | 11737506 |
| the microflora recovered from the outer-surfaces of the frialit-2 implanto-prosthetic connector. | the aim of the present investigation was to examine the outer-surface microbiota of the prosthetic connector of frialit-2 implants, and to compare the microbial findings with the peri-implant parameters 2 years after functional loading. in 16 implant-treated patients (55.8 +/- 9.5 years) the outer-surface micro-organisms of the prosthetic connectors were determined in 32 frialit-2 implants. the functional loading time of the prosthetic suprastructures was 24.1 +/- 13.8 months on average. after r ... | 2003 | 12562362 |
| effect of garenoxacin on eubacteria in the normal intestinal microflora when administered concomitantly with digoxin. | garenoxacin is a new des-f(6)-quinolone with a broad antimicrobial spectrum. it has been reported that antibiotics may raise digoxin concentrations in the plasma of patients who are taking these agents concurrently, possibly due to the effect on the digoxin-metabolizing intestinal microflora. sixteen healthy subjects were given a loading dose of digoxin (0.25 mg orally, q 6 h) on day 1 followed by once-daily doses of 0.25 mg on days 2 through 14. the subjects also received garenoxacin 600 mg ora ... | 2003 | 12868550 |
| in vitro activities of a new des-fluoro(6) quinolone, garenoxacin, against clinical anaerobic bacteria. | the antimicrobial activities of garenoxacin and eight other antibiotics against 641 anaerobic isolates were evaluated with the nccls agar dilution method. overall, the mics of garenoxacin for 50 and 90% of the strains tested (in micrograms per milliliter) were as follows: bacteroides fragilis group, 0.5 and 2; prevotella spp., 0.25 and 2; fusobacterium spp., 0.25 and 0.5; porphyromonas spp., 0.125 and 0.25; bilophila wadsworthia, 0.5 and 1; veillonella spp., 0.25 and 0.5; clostridium spp., 0.25 ... | 2003 | 14576144 |
| multiple bacteria in aortic aneurysms. | the purpose of the present study was to reexamine the possibility that bacteria, particularly anaerobes, are present in aortic aneurysms. | 2003 | 14681645 |