| degradation of 3-methylpyridine and 3-ethylpyridine by gordonia nitida le31. | cells of gordonia nitida le31 grown on 3-methylpyridine degraded 3-ethylpyridine without a lag time and vice versa. cyclic intermediates were not detected, but formic acid was identified as a metabolite. degradation of levulinic acid was induced in cells grown on 3-methylpyridine and 3-ethylpyridine. levulinic aldehyde dehydrogenase and formamidase activities were higher in cells grown on 3-methylpyridine and 3-ethylpyridine than in cells grown on acetate. these data indicate that 3-methylpyridi ... | 2001 | 11526043 |
| enhancement of phase separation by the addition of de-emulsifiers to three-phase (diesel oil/biocatalyst/aqueous phase) emulsion in diesel biodesulfurization. | ethanol, added as a de-emulsifier to separate oil and biocatalyst (or bacterial cells) from a three-phase (oil/biocatalyst/aqueous phase) emulsion, formed in diesel biodesulfurization employing gordonia nitida, improved oil recovery by centrifugation from about 50% in its absence to almost 100% at 3% (v/v). the biocatalyst recovered with ethanol addition showed similar specific growth rates (0.03 h(-1)) and dibenzothiophene desulfurization rates (6-7.2 mol l(-1) h(-1)) to those (0.03 h(-1) and 7 ... | 2003 | 12882310 |
| biocatalytic desulfurization of diesel oil in an air-lift reactor with immobilized gordonia nitida cyks1 cells. | a new type of air-lift reactor with immobilized gordonia nitida cyks1 cells on a fibrous support was designed and used for the biocatalytic desulfurization (bds) of diesel oil. its performance was evaluated at different phase ratios of the oil to the aqueous medium (or oil phase fractions) and different sucrose concentrations. when the reaction mixture contained 10% diesel oil (v/v), 61-67% of sulfur was removed as the sulfur content decreased from 202-250 to 76-90 mg l(-1) in 72 h. the sulfur c ... | 2005 | 15932256 |
| isolation and preliminary characterization of a respiratory nitrate reductase from hydrocarbon-degrading bacterium gordonia alkanivorans s7. | gordonia alkanivorans s7 is an efficient degrader of fuel oil hydrocarbons that can simultaneously utilize oxygen and nitrate as electron acceptors. the respiratory nitrate reductase (nar) from this organism has been isolated using ion exchange chromatography and gel filtration, and then preliminarily characterized. page, sds-page and gel filtration chromatography revealed that nar consisted of three subunits of 103, 53 and 25 kda. the enzyme was optimally active at ph 7.9 and 40 degrees c. k(m) ... | 2010 | 20379760 |
| ex situ bioremediation of oil-contaminated soil. | an innovative bioprocess method, systematic environmental molecular bioremediation technology (sembt) that combines bioaugmentation and biostimulation with a molecular monitoring microarray biochip, was developed as an integrated bioremediation technology to treat s- and t-series biopiles by using the landfarming operation and reseeding process to enhance the bioremediation efficiency. after 28 days of the bioremediation process, diesel oil (tph(c10-c28)) and fuel oil (tph(c10-c40)) were degrade ... | 2010 | 20053499 |
| biodesulfurization of dibenzothiophene by recombinant gordonia alkanivorans ripi90a. | the dszabc genes from newly reported dibenzothiophene biodesulfurizing bacterium, gordonia alkanivorans ripi90a were cloned and sequenced. the overall nucleotide sequence similarity between the dszabc genes of g. alkanivorans ripi90a and those of rhodococcus erythropolis igts8 and gordonia nitida were 83.1% and 83.2%, respectively. a gene transfer system for g. alkanivorans ripi90a was established employing the escherichia coli-rhodococcus shuttle vector prsg43 as suitable cloning vector, result ... | 2009 | 18653330 |
| dimethyl sulfoxide (dmso) as the sulfur source for the production of desulfurizing resting cells of gordonia alkanivorans ripi90a. | the sulfate repression of desulfurization (dsz) phenotype represents a major barrier to the mass production of desulfurizing resting cells. this repression can be avoided by replacing sulfate with dibenzothiophene (dbt) as the main substrate for the 4s pathway. however, mass production of biocatalyst using dbt is impractical because of its high price, low water solubility, and growth inhibition by 2-hydroxybiphenyl (2-hbp), which is the end product of the 4s pathway. in this work, the results sh ... | 2008 | 18310033 |
| jerusalem artichoke as low-cost fructose-rich feedstock for fossil fuels desulphurization by a fructophilic bacterium. | through biodesulphurization (bds) is possible to remove the sulphur present in fossil fuels to carry out the very strict legislation. however, this biological process is limited by the cost of the culture medium, and thus, it is important to explore cheaper alternative carbon sources, such as jerusalem artichoke (ja). these carbon sources usually contain sulphates which interfere with the bds process. the goal of this work was to remove the sulphates from jerusalem artichoke juice (jaj) through ... | 2015 | 25494982 |
| the genomes of the non-clearing-zone-forming and natural-rubber- degrading species gordonia polyisoprenivorans and gordonia westfalica harbor genes expressing lcp activity in streptomyces strains. | the latex-clearing protein (lcp(k30)) from the rubber-degrading bacterium streptomyces sp. strain k30 is involved in the cleavage of poly(cis-1,4-isoprene), yielding isoprenoid aldehydes and ketones. lcp homologues have so far been detected in all investigated clearing-zone-forming rubber-degrading bacteria. internal degenerated oligonucleotides derived from lcp genes of streptomyces sp. strain k30 (lcp(k30)), streptomyces coelicolor strain a3(2), and nocardia farcinica strains ifm10152 and e1 w ... | 2008 | 18296529 |
| influence of the carbon source on gordonia alkanivorans strain 1b resistance to 2-hydroxybiphenyl toxicity. | the viability of bacteria plays a critical role in the enhancement of fossil fuels biodesulfurization efficiency since cells are exposed to toxic compounds such as 2-hydroxybiphenyl (2-hbp), the end product of dibenzothiophene (dbt) biodesulfurization. the goal of this work was to study the influence of the carbon source on the resistance of gordonia alkanivorans strain 1b to 2-hbp. the physiological response of this bacterium, pre-grown in glucose or fructose, to 2-hbp was evaluated using two a ... | 2014 | 24728783 |
| exopolysaccharides produced by gordonia alkanivorans enhance bacterial degradation activity for diesel. | exopolysaccharides (eps) produced by gordonia alkanivorans cc-jg39 was used to stimulate cell floating, cell growth, and diesel biodegradation of indigenous or commercial-available, diesel-degrading bacteria. addition of eps-containing supernatant into the culture medium resulted in floatation of the non-floating bacteria and allowed a 40-45% and 38-42% increase in diesel degradation and cell growth, respectively. the eps-stimulating effect on cell growth and diesel degradation positively correl ... | 2008 | 18286235 |
| evidence for the role of zinc on the performance of dibenzothiophene desulfurization by gordonia alkanivorans strain 1b. | gordonia alkanivorans strain 1b is able to desulfurize dibenzothiophene (dbt) to 2-hydroxybiphenyl (2-hbp), the final product of the 4s pathway. however, both the cell growth and the rate of desulfurization can be largely affected by the nutrient composition of the growth medium due to cofactor requirements of many enzymes involved in the biochemical pathways. in this work, the effect of several metal ions on the growth and dbt desulfurization by g. alkanivorans was studied. from all the metal i ... | 2008 | 18043954 |
| dibenzothiophene desulfurization by gordonia alkanivorans strain 1b using recycled paper sludge hydrolyzate. | enzymatic hydrolyzates of recycled paper sludge were tested as suitable feedstock for biological desulfurization by gordonia alkanivorans strain 1b. only the hydrolyzate obtained after enzymatic mixture dialysis (dialyzed hydrolyzate) allowed dibenzothiophene (dbt) desulfurization, in spite of faster bacterial growth did occur on non-dialyzed hydrolyzate. for dialyzed hydrolyzate, 250microm dbt was consumed after 96h displaying a maximum specific productivity of 2-hydroxybiphenyl of 1.1micromol ... | 2008 | 17897697 |
| stabilization of water/gas oil emulsions by desulfurizing cells of gordonia alkanivorans ripi90a. | it has been previously reported that resting-cells, non-proliferating cells, of gordonia alkanivorans ripi90a can convert dibenzothiophene (dbt) to 2-hydroxybiphenyl (2-hbp) via the 4s pathway in a biphasic system. the main goal of the current work was to study the behaviour of resting-cells of this strain in biphasic organic media. resting-cells showed strong affinity for sulfurous organic substrates and were able to stabilize water/gas oil emulsions by attaching to the interface without decrea ... | 2007 | 17464072 |
| gordonia soli sp. nov., a novel actinomycete isolated from soil. | a soil isolate, strain cc-ab07t, was characterized using a polyphasic approach. this organism had chemotaxonomic and morphological properties consistent with its classification in the genus gordonia. 16s rrna gene sequence analysis showed that the novel strain formed a monophyletic branch at the periphery of the evolutionary radiation occupied by the genus gordonia, its closest neighbours being the type strains of gordonia alkanivorans, gordonia amicalis, gordonia bronchialis, gordonia desulfuri ... | 2006 | 17082398 |
| [comparison of the desulfurization activity among several bacteria and analysis of the conservation of their desulfurization genes]. | several bacteria, bacillus brevis r-6, pseudomonas delafleldii r-8, nocardia globerula r-9, bacillus sphaericus r-16, rhodococcus erythropolis lsse8-1 and gordonia nitida lssej-1, which can convert dibenzothiophene into 2-hydroxybiphenyl and sulfate, were investigated. desulfurization products were quantitively determined by hplc. result revealed that each of these bacteria desulfurize dbt at a different rate. in order to obtain more information, the fragments encoding desulfurizing enzymes were ... | 2005 | 16342766 |
| effect of the synthesized mycolic acid on the biodegradation of diesel oil by gordonia nitida strain le31. | the dynamics of diesel oil biodegradation were previously investigated at initial substrate concentrations of 1000 to 20,000 ppm using gordonia nitida isolated from wastewater. following the gas chromatogram profiles of diesel oil degradation, diesel oil with concentrations of up to 15,000 ppm was efficiently degraded by this strain. at a concentrations of 20,000 ppm, however, the degradation by this strain was not effective. the enhancement of the biodegradation of diesel oi1 (at 15,000 and 20, ... | 2005 | 16310733 |
| characterization of floating activity of indigenous diesel-assimilating bacterial isolates. | six diesel-degrading bacterial strains were isolated from oil-polluted sites located in central taiwan. the floating activity of the isolates in an oil-supplemented liquid medium was monitored. cell-surface hydrophobicity as well as cell-free and cell-residue emulsification activities were also investigated. three isolates, identified as gordonia alkanivorans cc-jg 39, rhodococcus erythropolis cc-bc 04, and r. erythropolis cc-bc 11, were found to float and grow near the diesel layer on the surfa ... | 2005 | 16233818 |
| rapid detection and identification of the metabolically diverse genus gordonia by 16s rrna-gene-targeted genus-specific primers. | the importance of the emerging genus gordonia in industrial and environmental biotechnology is evidenced by the recent increase in associated publications and patents. but, investigations into potentially valuable gordonia members are restricted by the limitations of current isolation and detection techniques. this motivated us to design a genus-specific oligonucleotide primer pair which could assist in rapid detection of species of the genus gordonia by means of pcr-specific amplification. the ... | 2005 | 16098691 |
| gordonia nitida yoon et al. 2000 is a later synonym of gordonia alkanivorans kummer et al. 1999. | the name of the species gordonia nitida is validly published but its type strain dsm 44499(t) shares high similarity based on 16s rrna gene sequences with gordonia alkanivorans dsm 44369(t) and gordonia westfalica dsm 44215(t). these three species obviously build up a distinct cluster within the genus gordonia. in the present paper, data from the literature concerning the three gordonia species were reviewed and the genetic similarity of g. nitida dsm 44499(t) and g. alkanivorans dsm 44369(t) wa ... | 2005 | 15774645 |
| desulfurization of dibenzothiophene, benzothiophene, and other thiophene analogs by a newly isolated bacterium, gordonia alkanivorans strain 1b. | a novel bacterium, gordonia alkanivorans strain 1b, was isolated from hydrocarbon-contaminated soil. assessment of the biodegradation of distinct organic sulfur-compounds, such as dibenzothiophene (dbt), benzothiophene (bt), dbt sulfone, and alkylated tiophenic compounds, as the sole source of sulfur was investigated. g. alkanivorans strain 1b was able to remove selectively the sulfur from dbt while keeping intact the remaining carbon-carbon structure. orthophenyl phenol (2-hydroxybiphenyl) was ... | 2005 | 15767694 |
| gordonia westfalica sp. nov., a novel rubber-degrading actinomycete. | a cis-1,4-polyisoprene-degrading bacterium (strain kb2t) was isolated from foul water taken from the inside of a deteriorated automobile tyre found on a farmer's field in westfalia, germany. the strain was aerobic, gram-positive, exhibited orange smooth and rough colonies on complex nutrient agar, produced elementary branching hyphae that fragmented into rod/coccus-like elements and showed chemotaxonomic markers which were consistent with its classification within the genus gordonia, i.e. the pr ... | 2002 | 12148617 |
| production of a desulfurization biocatalyst by two-stage fermentation and its application for the treatment of model and diesel oils. | for the production of oil-desulfurizing biocatalyst, a two-stage fermentation strategy was adopted, in which the cell growth stage and desulfurization activity induction stage were separated. sucrose was found to be the optimal carbon source for the growth of gordonia nitida cyks1. magnesium sulfate was selected to be the sulfur source in the cell growth stage. the optimal ranges of sucrose and magnesium sulfate were 10-50 and 1-2.5 g x l(-1), respectively. such a broad optimal concentration of ... | 2001 | 11587578 |
| gordonia amicalis sp. nov., a novel dibenzothiophene-desulphurizing actinomycete. | the taxonomic position of a dibenzothiophene-desulphurizing soil actinomycete was established using a polyphasic taxonomic approach. the organism, strain iegmt, was shown to have chemical and morphological properties typical of members of the genus gordonia. the tested strain formed a distinct phyletic line within the evolutionary radiation occupied by the genus gordonia, with gordonia alkanivorans dsm 44369t, gordonia desulfuricans ncimb 40816t and gordonia rubropertincta dsm 43197t as the most ... | 2000 | 11155977 |
| gordonia nitida sp. nov., a bacterium that degrades 3-ethylpyridine and 3-methylpyridine. | a bacterial strain, le31t, which is capable of degrading 3-ethylpyridine and 3-methylpyridine, was isolated from an industrial wastewater and was taxonomically studied by using a polyphasic approach. strain le31t was identified as a member of the genus gordonia on the basis of chemotaxonomic characteristics and phylogenetic inference-based 16s rdna sequence. the cell wall contained meso-diaminopimelic acid, arabinose and galactose (wall chemotype iv). the predominant menaquinone was mk-9(h2). th ... | 2000 | 10843064 |
| gordonia alkanivorans sp. nov., isolated from tar-contaminated soil. | twelve bacterial strains isolated from tar-contaminated soil were subjected to a polyphasic taxonomic study. the strains possessed meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan, mk-9(h2) as the predominant menaquinone, long-chain mycolic acids of the gordonia-type, straight-chain saturated and monounsaturated fatty acids, and considerable amounts of tuberculostearic acid. the g + c content of the dna was 68 mol%. chemotaxonomic and physiological properties and 16s ... | 1999 | 10555331 |
| degradation of di(2-ethylhexyl) phthalate by a novel gordonia alkanivorans strain yc-rl2. | one bacterial strain, yc-rl2, isolated from petroleum-contaminated soil, could utilize environmental hormone di(2-ethylhexyl) phthalate (dehp) as a sole carbon source for growth. strain yc-rl2 was identified as gordonia alkanivorans by 16s rrna gene analysis and biolog tests. the effects of environmental factors which might affect the degrading process were optimized at 30 °c and ph 8.0. strain yc-rl2 showed superior halotolerance and could tolerate up to 0-5% nacl in trace element medium supple ... | 2017 | 28078431 |
| production of ω-hydroxy palmitic acid using cyp153a35 and comparison of cytochrome p450 electron transfer system in vivo. | bacterial cytochrome p450 enzymes in cytochrome p450 (cyp)153 family were recently reported as fatty acid ω-hydroxylase. among them, cyp153as from marinobacter aquaeolei vt8 (cyp153a33), alcanivorax borkumensis sk2 (cyp153a13), and gordonia alkanivorans (cyp153a35) were selected, and their specific activities and product yields of ω-hydroxy palmitic acid based on whole cell reactions toward palmitic acid were compared. using camab as redox partner, cyp153a35 and cyp153a13 showed the highest prod ... | 2016 | 27344594 |
| Analysis of petroleum biodesulfurization in an airlift bioreactor using response surface methodology. | For the first time, growing cells of Gordonia alkanivorans RIPI90A were used for biodesulfurization (BDS) of diesel. This process was carried out in an internal airlift bioreactor. BDS parameters (oil/water phase ratio and initial sulfur concentration) were optimized in flasks using response surface methodology. Predicted results were found to be in good agreement with experimental results. Initial sulfur concentration had a remarkable effect on BDS process. Maximum removal of sulfur (21 mg/l) c ... | 2011 | 21945162 |
| toxicity evaluation of 2-hydroxybiphenyl and other compounds involved in studies of fossil fuels biodesulphurisation. | the acute toxicity of some compounds used in fossil fuels biodesulphurisation studies, on the respiration activity, was evaluated by gordonia alkanivorans and rhodococcus erythropolis. moreover, the effect of 2-hydroxybiphenyl on cell growth of both strains was also determined, using batch (chronic bioassays) and continuous cultures. the ic(50) values obtained showed the toxicity of all the compounds tested to both strains, specially the high toxicity of 2-hbp. these results were confirmed by th ... | 2011 | 21767949 |
| genomic structure and promoter analysis of the dsz operon for dibenzothiophene biodesulfurization from gordonia alkanivorans ripi90a. | the bacterium gordonia alkanivorans ripi90a has been previously reported as dibenzothiophene-desulfurizing strain. the present study provides a complete investigation of the dsz operon including dsz promoter analysis from desulfurization competent strain belonging to the genus gordonia. pcr was used to amplify the dszabc genes and adaptor ligation-based pcr-walking strategy used to isolate the dsz promoter. unlike the dsz operon of rhodococcus erythropolis, the operon of ripi90a was located on c ... | 2010 | 20414649 |
| simultaneously saccharification and fermentation approach as a tool for enhanced fossil fuels biodesulfurization. | biodesulfurization can be a complementary technology to the hydrodesulfurization, the commonly physical-chemical process used for sulfur removal from crude oil. the desulfurizing bacterium gordonia alkanivorans strain 1b as a fructophilic microorganism requires fructose as c-source. in this context, the main goal of this work was the optimization of a simultaneous saccharification and fermentation (ssf) approach using the zygosaccharomyces bailii strain talf1 crude enzymes with invertase activit ... | 2016 | 27505164 |
| locating and activating molecular 'time bombs': induction of mycolata prophages. | little is known about the prevalence, functionality and ecological roles of temperate phages for members of the mycolic acid producing bacteria, the mycolata. while many lytic phages infective for these organisms have been isolated, and assessed for their suitability for use as biological control agents of activated sludge foaming, no studies have investigated how temperate phages might be induced for this purpose. bioinformatic analysis using the phage search tool (phast) on mycolata whole geno ... | 2016 | 27487243 |
| production and characterization of a novel yeast extracellular invertase activity towards improved dibenzothiophene biodesulfurization. | the main goal of this work was the production and characterization of a novel invertase activity from zygosaccharomyces bailii strain talf1 for further application to biodesulfurization (bds) in order to expand the exploitable alternative carbon sources to renewable sucrose-rich feedstock. the maximum invertase activity (163 u ml(-1)) was achieved after 7 days of z. bailii strain talf1 cultivation at ph 5.5-6.0, 25 °c, and 150 rpm in yeast malt broth with 25 % jerusalem artichoke pulp as inducer ... | 2014 | 25163885 |
| enhancement of dibenzothiophene desulfurization by gordonia alkanivorans strain 1b using sugar beet molasses as alternative carbon source. | there are several problems limiting an industrial application of fossil fuel biodesulfurization, and one of them is the cost of culture media used to grow the microorganisms involved in the process. in this context, the utilization of alternative carbon sources resulting from agro-industrial by-products could be a strategy to reduce the investment in the operating expenses of a future industrial application. recently, gordonia alkanivorans 1b was described as a fructophilic desulfurizing bacteri ... | 2014 | 24519629 |
| draft genome sequence of gordonia alkanivorans strain cgmcc6845, a halotolerant hydrocarbon-degrading bacterium. | gordonia alkanivorans strain cgmcc6845 is a halotolerant hydrocarbon-degrading bacterium isolated from petroleum-contaminated saline soil. here we present the 5.0-mb draft genome sequence of this strain, which will improve our understanding of the diversity of g. alkanivorans and the mechanisms of microbial hydrocarbon degradation in saline environment. | 2014 | 24482528 |
| fructophilic behaviour of gordonia alkanivorans strain 1b during dibenzothiophene desulfurization process. | biodesulfurization (bds) aims at the removal of recalcitrant sulfur from fossil fuels at mild operating conditions with the aid of microorganisms. these microorganisms can remove sulfur from dibenzothiphene (dbt), a model compound, or other polycyclic aromatic used as sulfur source, making bds an easy and environmental friendly process. gordonia alkanivorans strain 1b has been described as a desulfurizing bacterium, able to desulfurize dbt to 2-hydroxybiphenyl (2-hbp), the final product of the 4 ... | 2014 | 24012483 |
| in silico modeling and evaluation of gordonia alkanivorans for biodesulfurization. | the genus gordonia is well known for its catabolic diversity and ability to transform several compounds including the various recalcitrant polyaromatic sulfur heterocycles (pashs) found in the fossil fuels. in fact, some strains offer the unique ability to desulfurize even benzothiophene (bt) and other thiophenic compounds, which most of the commonly studied rhodococci strains cannot. in this work, we present the first genome scale metabolic model for g. alkanivorans, a desulfurizing strain, to ... | 2013 | 23921469 |
| screening of novel yeast inulinases and further application to bioprocesses. | inulin is a carbohydrate composed of linear chains of β-2,1-linked d-fructofuranose molecules terminated by a glucose residue through a sucrose-type linkage at the reducing end. jerusalem artichoke (ja) is one of the most interesting materials among unconventional and renewable raw materials, with levels of inulin reaching 50-80% of dry matter. inulin or inulin-rich materials can be actively hydrolyzed by microbial inulinases to produce glucose and fructose syrups that can be used in bioprocesse ... | 2013 | 23419675 |
| metabolic roles of carotenoid produced by non-photosynthetic bacterium gordonia alkanivorans skf120101. | carotenoids produced by non-photosynthetic bacteria protect organisms against lethal photodynamic reactions and scavenge oxygenic radicals. however, the carotenoid produced by gordonia alkanivorans skf120101 is coupled to reducing power generation. skf120101 selectively produces carotenoid under light conditions. the growth yield of skf120101 cultivated under light conditions was higher than that under dark condition. in the cyclic voltammetry, both upper and lower voltammograms for neutral red ... | 2012 | 23124336 |