| nucleotide sequence analysis of a transposon (tn5393) carrying streptomycin resistance genes in erwinia amylovora and other gram-negative bacteria. | a class ii tn3-type transposable element, designated tn5393 and located on plasmid pea34 from streptomycin-resistant strain ca11 of erwinia amylovora, was identified by its ability to move from pea34 to different sites in plasmids pgem3zf(+) and pucd800. nucleotide sequence analysis reveals that tn5393 consists of 6,705 bp with 81-bp terminal inverted repeats and generates 5-bp duplications of the target dna following insertion. tn5393 contains open reading frames that encode a putative transpos ... | 1993 | 8380801 |
| characterization of plasmids that encode streptomycin-resistance in bacterial epiphytes of apple. | streptomycin resistance in strains of pseudomonas syringae pv. papulans, pantoea agglomerans and a yellow-pigmented, non-fluorescent pseudomonas sp. (py), isolated from apple orchards in new york and washington states, is predominantly associated with stra-strb genes carried on conjugal plasmids (r plasmids). none of 128 resistant erwinia amylovora strains from the eastern and western usa hybridized with a stra-strb probe, smp3. resistant py strains transfered r plasmids to ps. syringae pv. papu ... | 1999 | 10347868 |
| homologous streptomycin resistance gene present among diverse gram-negative bacteria in new york state apple orchards. | the streptomycin resistance gene of pseudomonas syringae pv. papulans psp36 was cloned into escherichia coli and used to develop a 500-bp dna probe that is specific for streptomycin resistance in p. syringae pv. papulans. the probe is a portion of a 1-kb region shared by three different dna clones of the resistance gene. in southern hybridizations, the probe hybridized only with dna isolated from streptomycin-resistant strains of p. syringae pv. papulans and not with the dna of streptomycin-sens ... | 1991 | 16348415 |
| rapid diagnosis of pseudomonas syringae pv. papulans, the causal agent of blister spot of apple, by polymerase chain reaction using specifically designed hrpl gene primers. | abstract the identification and detection of pseudomonas syringae pv. papulans, the causal agent of blister spot of apple, on apple leaves and fruit was achieved by polymerase chain reaction amplification of a specific dna fragment of the hrpl sequence. the consensus primers hrpl(1) and hrpl(2) were designed based on the alignment of pseudomonad hrpl gene sequences available in nucleic acid data banks. this primer set produced a 631-bp amplicon from 37 of the 57 pseudomonads strains tested. thes ... | 2002 | 18944218 |