| interspecific differentiation of trypanosoma cruzi, trypanosoma conorhini and trypanosoma rangeli by lectins in combination with complement lysis. | four-day-old epimastigote culture forms of trypanosoma cruzi, trypanosoma rangeli and trypanosoma conorhini were tested with 21 lectins. furthermore t. conorhini was incubated with the following sera: rat, wistar han, germ free; normal fresh hen, rat and human serum. t. rangeli was agglutinated only by the d-mannose specific lectins from canavalia ensiformis and pisum sativum. t. cruzi and t. conorhini could be distinguished by the lectin from tridacna crocea. the epimastigote culture forms of t ... | 1984 | 6143480 |
| survey of antitrypanosome antibodies and studies of their specificity and autoimmunity. | as a part of investigations to characterize trypanosome infections in taiwan, sera collected from patients admitted to veterans general hospital, taipei were tested for antitrypanosome antibodies. a trypanosoma cruzi extract-based enzyme-linked immunosorbent assay (elisa) was used to screen and titrate 1,297 patient sera. high antitrypanosome titers were detected in 166 (12.8%) of these sera. retitration of random samples of the high titer (ht) sera indicated a 5.4% false positive. thirteen dono ... | 1995 | 9775001 |
| biochemical properties of trypanosomatid lactate dehydrogenases. | lactate dehydrogenase (ldh, e.c.1.1.1.27) was found in supernatant (cytoplasmic enzyme) fractions of the trypanosomatid flagellates trypanosoma conorhini and crithidia fasciculata if 10 mm cysteine was present in the homogenizing medium. the t. conorhini ldh activity with pyruvate as substrate was increased 35% if 5 mm cysteine was also included in reaction mixtures. k(m) values for the t. conorhini enzyme were 3.3 x 10(-4)m with pyruvate, and 1.6 x 10(-4)m with alpha-ketobutyrate. cysteine inhi ... | 1970 | 5464273 |
| temperature gradient gel electrophoresis (tgge) analysis of riboprints from trypanosoma cruzi. | to test the homogeneity of 18s sequences within trypanosoma cruzi, riboprint profiles were separated by temperature gradient gel electrophoresis (tgge). upon interpretation of melting curves of fragments within a riboprint profile, there appeared to be two 18s sequence types within each stock examined. two similar types were also observed within outgroup taxa trypanosoma conorhini, trypanosoma rangeli and leishmania braziliensis. from dna hybridization studies, these fragments were shown to have ... | 1998 | 9774790 |
| homologues of the 24-kda flagellar ca(2+)-binding protein gene of trypanosoma cruzi are present in other members of the trypanosomatidae family. | a full-length cdna encoding the 24-kda flagellar ca(2+)-binding protein (fcabp) of the dm28c clone of trypanosoma cruzi was cloned and characterized. comparison of the deduced amino acid sequence with those of the fcabps of other t. cruzi strains revealed greater than 97% sequence conservation. fcabp-like genes are found in trypanosoma conorhini, trypanosoma freitasi, trypanosoma lewisi, herpetomonas megaseliae, leptomonas seymouri, and phytomonas serpens, but not in crithidia deanei, leishmania ... | 1997 | 9225770 |
| trans-sialidase and sialidase activities discriminate between morphologically indistinguishable trypanosomatids. | the expression of trans-sialidase and sialidase activities in the kinetoplastid protozoa was explored as a potential marker to discriminate between the morphologically indistinguishable flagellates isolated from human, insects and vertebrate reservoir hosts. by virtue of the differences observed in the ratios of these enzyme activities, a collection of 52 species and strains comprising the major taxa of these parasites could be separated into four expression types. type-i parasites express compa ... | 1994 | 7925453 |
| lectins discriminate between pathogenic and nonpathogenic south american trypanosomes. | cell surface carbohydrates of trypanosoma cruzi, trypanosoma rangeli, and trypanosoma conorhini were analyzed by a micro-agglutination assay employing 27 highly purified lectins and by binding assays using various 125i-labeled lectins. the following seven lectins discriminated between the trypanosomes: 1) tomato lectin (an n-acetyl-d-glucosamine-binding protein), both in purified form and as crude tomato juice; 2) bauhinea purpurea and sophora japonica lectins (both n-acetyl-d-galactosamine-bind ... | 1984 | 6385741 |
| structural features and antigenic properties of carbohydrate-containing components of trypanosoma conorhini. | aqueous and phenolic extracts of trypanosoma conorhini were fractionated and high molecular weight, carbohydrate-rich fractions obtained. their antigenic characteristics, reactivity with lectins and partial chemical structure were determined. the major component, the phenolic extract, was electrophoretically diffuse and consisted of 15% protein, 5% phosphorus, hexosamine, and 67% neutral carbohydrate, which contained mannose, galactose, and xylose in a molar ratio of 1.0:1.8:1.8. chemical analys ... | 1987 | 3123927 |
| characterization of protein-linked oligosaccharides in trypanosomatid flagellates. | protein-linked, endo-beta-n-acetylglucosaminidase h-sensitive oligosaccharides were isolated from several trypanosomatids incubated with [u-14c]glucose. structural analysis of the compounds revealed that man9glcnac2 was the oligosaccharide transferred from dolichol-p-p derivatives to proteins in trypanosoma dionisii, trypanosoma conorhini, leptomonas samueli and herpetomonas samuelpessoai and man6glcnac2 in blastocrithidia culicis and leishmania adleri. in all cases, transiently glucosylated com ... | 1986 | 3083255 |
| characterization of dolichol monophosphate- and dolichol diphosphate-linked saccharides in trypanosomatid flagellates. | dolichol-p- and dolichol-p-p-linked saccharides were isolated from several trypanosomatid flagellates incubated with [u-14c]glucose. formation of glc-p-dolichol and man-p-dolichol was observed in herpetomonas muscarum and leishmania adleri, whereas only the latter derivative was synthesized in trypanosoma dionisii and leptomonas samueli. the main and largest dolichol-p-p-linked oligosaccharide formed in trypanosoma conorhini, t. dionisii, l. samueli, herpetomonas samuelpessoai and h. muscarum ap ... | 1986 | 3083254 |
| restriction fragment length polymorphisms in the ribosomal gene spacers of trypanosoma cruzi and trypanosoma conorhini. | the ribosomal rna genes of two species of trypanosoma, trypanosoma cruzi, the etiological agent of chagas' disease, and trypanosoma conorhini, a non-pathogenic rodent trypanosome, were cloned and partially characterized. the physical maps derived for their rrna genes were similar throughout the region that encompasses the ssu-and lsu-rrna coding sequences. however, the non-transcribed spacer dna of both t. cruzi and t. conorhini was found to be polymorphic for several restriction enzyme sites. w ... | 1990 | 1978249 |
| the development and survival of the blood-stream forms of trypanosoma conorhini in culture. | | 1963 | 14074432 |
| life-cycle of trypanosoma conorhini. influence of temperature and other factors on growth and morphogenesis. | | 1963 | 14074433 |
| ultrastructure of the "cyst-like bodies" of trypanosoma conorhini. | | 1972 | 4332907 |
| the diversity and expansion of the trans-sialidase gene family is a common feature in trypanosoma cruzi clade members. | trans-sialidase (ts) is a polymorphic protein superfamily described in members of the protozoan genus trypanosoma. of the eight ts groups recently described, ts group i proteins (some of which have catalytic activity) are present in the distantly related trypanosoma brucei and trypanosoma cruzi phylogenetic clades, whereas other ts groups have only been described in some species belonging to the t. cruzi clade. in the present study we analyzed the repertoire, distribution and phylogenetic relati ... | 2016 | 26640033 |
| trypanosoma cruzi oleate desaturase: molecular characterization and comparative analysis in other trypanosomatids. | trypanosoma cruzi lipids contain a high content of unsaturated fatty acids, primarily oleic acid (c18:1) and linoleic acid (c18:2). previous data suggest that this parasite is able to convert oleic acid into linoleic acid; humans are not able to do this. presently, we show that t. cruzi has a gene with high similarity to the delta12 (omega6)-oleate desaturase from plants. northern blot analysis of the oleate desaturase gene from t. cruzi (od(tc)) indicated that this gene is transcribed in epimas ... | 2006 | 17152952 |
| are asian monkeys the original mammalian hosts of trypanosoma conorhini? | it is hypothesized that asian monkeys were the original hosts of trypanosoma conorhini because they have been found naturally infected, the vector among rats is a tropicopolitan triatomine bug that belongs to a complex of asian species, and primates were shown to be more susceptible than rats. | 1986 | 3796277 |