| alkaline phosphatase activity of rumen bacteria. | of the 54 strains of rumen bacteria examined for alkaline phosphatase (apase) production, 9 of 33 gram-negative strains and none of 21 gram-positive strains produced the enzyme. the apase of the cells of the three strains of bacteroides ruminicola that produced significant amounts of the enzyme was located in the periplasmic area of the cell envelope, whereas the enzyme was located in the strains of selenomonas ruminantium and succinivibrio dextrinosolvens was associated with the outer membrane. ... | 1977 | 563216 |
| anaerobic vibrio-like organisms cultured from blood: desulfovibrio desulfuricans and succinivibrio species. | two unusual anaerobic vibrio-like organisms were recovered from blood cultures of two patients. one isolate was identified as desulfovibrio desulfuricans. it appeared to be the cause of a 24-h episode of fever, chills, and profuse perspiration. this is apparently the first documented report of human infection due to this organism. the second isolate was a succinivibrio species. it has rarely been described as a cause of bacteremia. the clinical significance of the organism remains unclear. | 1977 | 858783 |
| urease assay and urease-producing species of anaerobes in the bovine rumen and human feces. | a growth medium and test were developed for rapid detection of urease in fermentative anaerobic bacteria. using nonselective rumen fluid roll-tube agar medium and the new test, it was confirmed that peptostreptococcus productus is often the most numerous urease-forming species in human feces. also, some fecal strains of ruminococcus albus, clostridium innocuum, and clostridium beijerinckii produced urease. single strains of fusobacterium prausnitzii, coprococcus catus, and streptococcus mitis th ... | 1977 | 879770 |
| bacteremia due to succinivibrio dextrinosolvens. report of a case. | an unusual case report of a patient with bacteremia due to succinivibrio dextrinosolvens is presented. heretofore, succinivibrio spp. were thought to occur only as organisms in the rumens of herbivorous animals. succinivibrio is an anaerobic, gram-negative, curved, spiral bacillus with a polar monotrichous flagellar pattern. volatile acids produced from glucose metabolism include succinic, acetic, formic and lactic. various carbohydrate substrates are fermented to produce strong or weak acid end ... | 1975 | 1199976 |
| frequent hospital admissions for bacterial infections among aboriginal people with diabetes in central australia. | to determine hospital-based morbidity and mortality from bacterial infections among aboriginal people with diabetes in central australia. | 1991 | 1875833 |
| [production of fibroblast chemotactic factors from root canal bacteria]. | it is well known that periapical pathosis is one of endogenous infections caused by indigenous bacteria in the oral cavity. therefore, interaction of host and parasite factors affect the progress of the lesion. in host factors, fibroblasts migrate chemotactically, proliferate and constitute new connective tissues at a late stage of the inflammation process. all of chemoattractants for fibroblasts previously reported are derived from the host. in this study, fibroblast chemotactic activities in b ... | 1989 | 2519268 |
| glutamine synthetase activity in the ruminal bacterium succinivibrio dextrinosolvens. | succinivibrio dextrinosolvens c18 was found to possess glutamine synthetase (gs), urease, glutamate dehydrogenase, and several other nitrogen assimilation enzymes. when grown in continuous culture under ammonia limitation, both gs and urease activities were high and glutamate dehydrogenase activity was low, but the opposite activity pattern was observed for growth in the presence of ample ammonia. the addition of high-level (15 mm) ammonium chloride to ammonia-limited cultures resulted in a rapi ... | 1985 | 2867738 |
| uncommonly encountered, motile, anaerobic gram-negative bacilli associated with infection. | motile, anaerobic gram-negative bacilli belonging to the genera butyrivibrio, succinimonas, succinivibrio, anaerovibrio, wolinella, campylobacter, desulfovibrio, selenomonas, and anaerobiospirillum are being recognized in clinical specimens with increasing frequency. over a 12.5-year period at the va wadsworth medical center, 13 clinical specimens yielded one of these organisms. six isolates were recovered from infected wounds, five from respiratory tract specimens obtained from patients with an ... | 1987 | 3321364 |
| antibiotic activity of an isocyanide metabolite of trichoderma hamatum against rumen bacteria. | a metabolite of trichoderma hamatum, 3-(3-isocyanocyclopent-2-enylidene)propionic acid, was tested for its effects on growth of and carbohydrate metabolism in 11 strains of functionally important rumen bacteria. to standardize the biological activity of this unstable metabolite, a rapid, aerobic disc diffusion assay was developed using escherichia coli atcc 11775. in an anaerobic broth dilution assay using a medium lacking rumen fluid and containing a soluble carbohydrate, the minimum inhibitory ... | 1985 | 4084861 |
| oxygen sensitivity of various anaerobic bacteria. | anaerobes differ in their sensitivity to oxygen, as two patterns were recognizable in the organisms included in this study. strict anaerobes were species incapable of agar surface growth at po(2) levels greater than 0.5%. species that were found to be strict anaerobes were treponema macrodentium, treponema denticola, treponema oralis n. sp., clostridium haemolyticum, selenomonas ruminatium, butyrivibrio fibrisolvens, succinivibrio dextrinosolvens, and lachnospira multiparus. moderate anaerobes w ... | 1969 | 5370458 |
| carbon dioxide requirement of various species of rumen bacteria. | the carbon dioxide requirement of 32 strains of rumen bacteria, representing 11 different species, was studied in detail. increasing concentrations of co(2) were added as nahco(3) to a specially prepared co(2)-free medium which was tubed and inoculated under nitrogen. prior depletion of co(2) in the inoculum was found to affect the level of requirement; however, the complexity and buffering capacity of the medium did not appear to be involved. an absolute requirement for co(2) was observed for e ... | 1971 | 5541030 |
| a note on the fermentation of pectin by pure strains and combined cultures of rumen bacteria. | bacteroides ruminicola, pure or combined with selenomonas ruminantium, and lachnospira multiparus, pure or combined with succinivibrio dextrinosolvens, were grown on a medium with pectin as energy source. there was a difference in fermentation products between the pure and combined cultures and efficiency of substrate utilization was better with the combined cultures. | 1981 | 6168176 |
| isolation and presumptive identification of adherent epithelial bacteria ("epimural" bacteria) from the ovine rumen wall. | one hundred sixty-one strains of adherent bacteria were isolated under anaerobic conditions from four sites on the rumen epithelial surface of sheep fed hay or a hay-grain ration. before isolation of bacteria, rumen tissue was washed six times in an anaerobic dilution solution, and viable bacteria suspended in the washings were counted. calculation indicated that unattached bacteria would have been removed from the tissue by this procedure, but a slow and progressive release of attached bacteria ... | 1981 | 7195191 |
| effects of lasalocid or monensin on lactate-producing or -using rumen bacteria. | lasalocid or monensin inhibited most of the lactate-producing rumen bacteria (butyrivibrio fibrisolvens, eubacterium cellulosolvens, e. ruminantium, lachnospira multiparus, lactobacillus ruminis, l. vitulinus, ruminococcus albus, r. flavefaciens, streptococcus bovis). minimum inhibitory concentrations ranged from .38 to 3.0 micrograms/ml. among the lactate producers, those that produce succinate as a major end product (bacteroides, selenomonas, succinimonas, succinivibrio) were not inhibited by ... | 1981 | 7275867 |
| glucose and carbon dioxide metabolism by succinivibrio dextrinosolvens. | growth rates and culture conditions affect the molar yields of catabolic end products and cells of succinivibrio dextrinosolvens growing on glucose. when growth in chemostats occurred, a trend toward decreased succinate and acetate formation, increased lactate formation, and a higher yield of cells correlated with an increase in the growth rate. end product and cellular yields on defined medium indicate a high maintenance requirement for s. dextrinosolvens and are consistent with energy conserva ... | 1993 | 8481001 |
| diet-dependent shifts in the bacterial population of the rumen revealed with real-time pcr. | a set of pcr primers was designed and validated for specific detection and quantification of prevotella ruminicola, prevotella albensis, prevotella bryantii, fibrobacter succinogenes, selenomonas ruminantium-mitsuokella multiacida, streptococcus bovis, ruminococcus flavefaciens, ruminobacter amylophilus, eubacterium ruminantium, treponema bryantii, succinivibrio dextrinosolvens, and anaerovibrio lipolytica. by using these primers and the real-time pcr technique, the corresponding species in the ... | 2001 | 11375193 |
| detection of proteobacteria from the rumen by pcr using methanotroph-specific primers. | to detect proteobacteria, including methanotrophs, from the rumen fluid and the bacteria inhabiting the rumen epithelium. | 2002 | 12180951 |
| dominance of prevotella and low abundance of classical ruminal bacterial species in the bovine rumen revealed by relative quantification real-time pcr. | relative quantification real-time pcr was used to quantify several bacterial species in ruminal samples from two lactating cows, each sampled 3 h after feeding on two successive days. abundance of each target taxon was calculated as a fraction of the total 16s rrna gene copies in the samples, using taxon-specific and eubacterial domain-level primers. bacterial populations showed a clear predominance of members of the genus prevotella, which comprised 42% to 60% of the bacterial rrna gene copies ... | 2007 | 17235560 |
| biotransformation of 2,4,6-trinitrotoluene by pure culture ruminal bacteria. | twenty-one ruminal bacteria species were tested for their ability to degrade 2,4,6-trinitrotoluene (tnt) within 24 h. butyrivibrio fibrisolvens, fibrobacter succinogenes, lactobacillus vitulinus, selenomonas ruminantium, streptococcus caprinus, and succinivibrio dextrinosolvens were able to completely degrade 100 mg/l tnt, with <5% of the original tnt recovered as diaminonitrotoluene metabolites. eubacterium ruminantium, lactobacillus ruminis, ruminobacter amylophilus, streptococcus bovis, and w ... | 2009 | 18839246 |
| the effect of replacing corn with glycerol on ruminal bacteria in continuous culture fermenters. | the effects of substituting corn with glycerol on dna concentration of selected ruminal bacteria were investigated using continuous fermenters. four continuous culture fermenters were used in a 4 × 4 latin square design with four 10 days consecutive periods. treatment diets (60:40 forage to concentrate) were fed at 45 g/day dry matter (dm) in three equal portions. glycerol (0.995 g/g glycerol) was used to replace corn in a grain mix at proportions of 0% (t0; control), 15% (t15), 30% (t30) and 45 ... | 2010 | 20880288 |
| bacterial community analysis of swine manure treated with autothermal thermophilic aerobic digestion. | due to the environmental problems associated with disposal of livestock sludge, many stabilization studies emphasizing on the sludge volume reduction were performed. however, little is known about the microbial risk present in sludge and its stabilized products. this study microbiologically explored the effects of anaerobic lagoon fermentation (alf) and autothermal thermophilic aerobic digestion (atad) on pathogen-related risk of raw swine manure by using culture-independent 16s rdna cloning and ... | 2010 | 20922382 |
| fermentation of xylans by butyrivibrio fibrisolvens and other ruminal bacteria. | the ability of butyrivibrio fibrisolvens and other ruminal bacteria (6 species, 18 strains) to ferment a crude xylan from wheat straw or to ferment xylans from larchwood or oat spelts was studied. liquid cultures were monitored for carbohydrate utilization, cell growth (protein), and fermentation acid production. b. fibrisolvens 49, h17c, actf2, and d1 grew almost as well on one or more of the xylans as they did on cellobiose-maltose. b. fibrisolvens 12, r28, a38, x10c34, ard22a, and x6c61 exhib ... | 1987 | 3124741 |
| Impact of Feed Efficiency and Diet on the Adaptive Variations in the Bacterial Community in the Rumen Fluid of Cattle Identified by PCR-DGGE and Quantitative Real Time PCR Analysis. | Limited knowledge of the structure and activities of the ruminal bacterial community prevents understanding of the impact of population dynamics on functional bacterial groups and on host productivity. This study aimed to identify particular bacteria associated with host feed efficiency in steers differing diets and residual feed intake (RFI) using culture-independent methods: PCR-denaturing gradient gel electrophoresis (DGGE) and quantitative real time PCR analysis. PCR-DGGE profiles were gener ... | 2011 | 22156428 |
| molecular analysis of bacterial communities in uranium ores and surrounding soils from banduhurang open cast uranium mine, india: a comparative study. | bacterial community structure of heavy metal rich- uranium ores and surrounding soils was explored using 16s rrna gene based clone library analysis and denaturing gradient gel electrophoresis (dgge) to provide baseline microbial diversity data on autochthonous communities. sequence analysis of major ribotypes and/or dgge bands revealed proteobacteria and acidobacteria as the two most frequently present bacterial phyla across the samples, although relative abundance of each phyla and identity of ... | 2011 | 21308598 |
| characterization of egg yolk antibodies for detection and quantification of selenomonas ruminantium by using an enzyme-linked immunosorbent assay. | the specificity of polyclonal antibodies prepared against strains of selenomonas ruminantium, the effect of assay conditions, and quantification of individual strains in mixed-cell suspensions of selenomonad strains were examined in this study. whole-cell suspensions were prepared with pure cultures of s. ruminantium pc18, hd(4), ga192, and d. each cell suspension was injected into a leghorn laying hen, and polyclonal antibodies were harvested from eggs laid in week 3 or 7 following initial immu ... | 1990 | 16348287 |
| isolation, enumeration, and characteristics of proteolytic ruminal bacteria. | fulghum, robert s. (virginia polytechnic institute, blacksburg) and w. e. c. moore. isolation, enumeration, and characteristics of proteolytic ruminal bacteria. j. bacteriol. 85:808-815. 1963.-colony counts of proteolytic ruminal bacteria in the order of 10(9) organisms per g of whole rumen contents, and total colony counts in the order of 2 to 3 x 10(9) organisms per g, were obtained from rumen contents of cattle fed a maintenance ration of hay and grain. the proteolytic counts averaged 38% of ... | 1963 | 14044947 |
| phylogenetic analysis of formivibrio citricus, propionivibrio dicarboxylicus, anaerobiospirillum thomasii, succinimonas amylolytica and succinivibrio dextrinosolvens and proposal of succinivibrionaceae fam. nov. | the phylogenetic position of gram-negative, strictly anaerobic, non-spore-forming bacteria, representing four different genera, was determined by analysis of their 16s rdna sequences. formivibrio citricus and propionivibrio dicarboxylicus are members of the beta-subclass of the class proteobacteria. while formivibrio citricus stands phylogenetically isolated, propionivibrio dicarboxylicus is moderately related to members of the genus rhodocyclus. succinimonas amylolytica and succinivibrio dextri ... | 1999 | 10319502 |
| 1,4-naphthoquinone and other nutrient requirements of succinivibrio dextrinosolvens. | three strains of succinivibrio dextrinosolvens isolated from the rumen of cattle or sheep under diverse conditions grew well in a minimal medium containing glucose, minerals, cysteine, methionine, leucine, serine, ammonia, 1,4-naphthoquinone, p-aminobenzoic acid, and bicarbonate-carbonic acid buffer, ph 6.7. when menadione or vitamin k5 was substituted for 1,4-naphthoquinone, the growth rate was somewhat depressed. growth was poor with vitamin k1 and ammonia, further addition of the amino acids ... | 1982 | 7125652 |
| effects of chetomin on growth and acidic fermentation products of rumen bacteria. | chetomin, an antibiotic metabolite of chaetomium spp., was tested in the form of its tetrathiol derivative for its effects on growth and carbohydrate metabolism by five strains of functionally important rumen bacteria. the compound was bacteriostatic for the strains tested and gram-positive bacteria were more sensitive to inhibition than gram-negative bacteria. in an anaerobic broth dilution assay using a medium lacking rumen fluid, the minimum inhibitory concentration (mic) of chetomin which co ... | 1983 | 6686488 |
| antigenic distinctiveness of mobiluncus curtisii and mobiluncus mulieris. | a total of 26 mobiluncus strains (17 m. curtisii and 9 m. mulieris strains) were compared serologically by double immunodiffusion and immunoblotting against antisera prepared against representative isolates of each species. all strains from the same species were strongly reactive with homologous antisera but generally weakly reactive with antisera to the heterologous mobiluncus spp. the antisera did not react with strains of the unrelated genera campylobacter, succinivibrio, wolinella, actinomyc ... | 1985 | 3924951 |