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characterization of n2o-producing xanthomonas-like isolates from biofilters as stenotrophomonas nitritireducens sp. nov., luteimonas mephitis gen. nov., sp. nov. and pseudoxanthomonas broegbernensis gen. nov., sp. nov.a group of yellow-pigmented isolates from ammonia-supplied biofilters showed an unusual denitrification reaction. all strains reduced nitrite but not nitrate without production of nitrogen (n2). the only product found was nitrous oxide (n2o). the strains were divided into two clusters and one separate strain by their fatty acid profiles, which were similar to the fatty acid profiles of the genera xanthomonas and stenotrophomonas. analyses of the 165 rdna sequences showed that these clusters and ...200010826814
substrate specificity of stenotrophomonas nitritireducens in the hydroxylation of unsaturated fatty acid.an isolated bacterium that converted unsaturated fatty acids to hydroxy fatty acids was identified as stenotrophomonas nitritireducens by api analysis, cellular fatty acids compositions, sequencing the full 16s ribosomal ribonucleic acid, and evaluating its nitrite reduction ability. s. nitritireducens has unique regio-specificity for c16 and c18 cis-9 unsaturated fatty acids. these fatty acids are converted to their 10-hydroxy fatty acids without detectable byproducts. among the cis-9-unsaturat ...200818060400
conversion of oleic acid to 10-hydroxystearic acid by whole cells of stenotrophomonas nitritireducens.the optimal reaction conditions for the conversion of oleic acid to 10-hydroxystearic acid by whole cells of stenotrophomonas nitritireducens were: ph 7.5, 35 °c, 0.05% (w/v) tween 80, 20 g cells l(-1), and 30 g oleic acid l(-1) in an anaerobic atmosphere. under these conditions, the cells produced 31.5 g 10-hydroxystearic acid l(-1) over 4 h with a conversion yield of 100% (mol/mol) and a productivity of 7.9 g l(-1) h(-1), indicating that oleic acid was converted completely to 10-hydroxystearic ...201121207107
characterization of a new keratin-degrading bacterium isolated from deer fur.a keratin-degrading bacterium was isolated from soil containing deer fur. an axenic culture of the keratin-degrading bacterium was obtained in liquid culture using a keratin enrichment technique. the isolated bacterium was gram negative and catalase- and oxidase-positive. transmission electron microscopic observations showed that the bacterium was rod-shaped, 1.0-1.3 microm long and 0.7 microm in diameter. phylogenetic analysis of 16s rdna revealed that the new isolate has only 90.6% homology wi ...200216233255
bacterium-based no2- biosensor for environmental applications.a sensitive no2- biosensor that is based on bacterial reduction of no2- to n2o and subsequent detection of the n2o by a built-in electrochemical n2o sensor was developed. four different denitrifying organisms lacking no3- reductase activity were assessed for use in the biosensor. the relevant physiological aspects examined included denitrifying characteristics, growth rate, no2- tolerance, and temperature and salinity effects on the growth rate. two organisms were successfully used in the biosen ...200415528518
stenotrophomonas acidaminiphila sp. nov., a strictly aerobic bacterium isolated from an upflow anaerobic sludge blanket (uasb) reactor.two of several strictly aerobic, mesophilic bacteria isolated from a lab-scale upflow anaerobic sludge blanket (uasb) reactor treating a petrochemical wastewater, strains amx 17 and amx 19t, were subjected to detailed taxonomic study. cells were gram-negative, motile, non-sporulating, straight to curved rods with a polar flagellum. the isolates exhibited phenotypic traits of members of the genus stenotrophomonas, including cellular fatty acid composition and the limited range of substrates that ...200211931169
bacteria-mediated aerobic degradation of hexacosane in vitro conditions.in vitro degradation of hexacosane (c26h54), a hmw n-alkane, was studied in msm by two bacterial strains i.e., pseudomonas sp. bp10 and stenotrophomonas nitritireducens e9, isolated from petroleum sludge, in isolation and combination. the results revealed that both the strains were able to metabolize hexacosane by 82% in isolation and 98% in their consortium after 7days. an enhancement of 16% in hexacosane degradation by the consortium indicated an additive action of bacterial strains. however, ...201425125193
production of 10-hydroxy-12,15(z,z)-octadecadienoic acid from α-linolenic acid by permeabilized stenotrophomonas nitritireducens cells.to improve the production of 10-hydroxy-12,15(z,z)-octadecadienoic acid (hoda) from α-linolenic acid in stenotrophomonas nitritireducens.201526188993
gene cloning of an efficiency oleate hydratase from stenotrophomonas nitritireducens for polyunsaturated fatty acids and its application in the conversion of plant oils to 10-hydroxy fatty acids.hydroxy fatty acids are used as precursors of lactones and dicarboxylic acids, as starting materials of polymers, and as additives in coatings and paintings. stenotrophomonas nitritireducens efficiently converts cis-9 polyunsaturated fatty acids (pufas) to 10-hydroxy fatty acids. however, gene encoding enzyme involved in this conversion has not been identified to date. we purified a putative fatty acid double-bond hydratase from s. nitritireducens by ultrafiltration and hiprep deae ff and resour ...201727474883
γ-dodecelactone production from safflower oil via 10-hydroxy-12(z)-octadecenoic acid intermediate by whole cells of candida boidinii and stenotrophomonas nitritireducens.candida boidinii was selected as a γ-dodecelactone producer because of the highest production of γ-dodecelactone from 10-hydroxy-12(z)-octadecenoic acid among the 11 yeast strains tested. under the reaction conditions of ph 5.5 and 25 °c with 5 g/l 10-hydroxy-12(z)-octadecenoic acid and 30 g/l cells, whole c. boidinii cells produced 2.1 g/l γ-dodecelactone from 5 g/l 10-hydroxy-12(z)-octadecenoic acid after 6 h, with a conversion yield of 64% (mol/mol) and a volumetric productivity of 350 mg/l/h ...201424967938
screening for endophytic nitrogen-fixing bacteria in brazilian sugar cane varieties used in organic farming and description of stenotrophomonas pavanii sp. nov.a gram-negative, rod-shaped, non-spore-forming and nitrogen-fixing bacterium, designated icb 89(t), was isolated from stems of a brazilian sugar cane variety widely used in organic farming. 16s rrna gene sequence analysis revealed that strain icb 89(t) belonged to the genus stenotrophomonas and was most closely related to stenotrophomonas maltophilia lmg 958(t), stenotrophomonas rhizophila lmg 22075(t), stenotrophomonas nitritireducens l2(t), [pseudomonas] geniculata atcc 19374(t), [pseudomonas] ...201120495025
stenotrophomonas daejeonensis sp. nov., isolated from sewage.a gram-stain-negative, motile, aerobic bacterial strain, designated mj03(t), was isolated from sewage and was characterized taxonomically by using a polyphasic approach. comparative 16s rrna gene sequence analysis showed that strain mj03(t) belongs to the family xanthomonadaceae, class gammaproteobacteria, and was related most closely to stenotrophomonas acidaminiphila amx 19(t) (97.9  % sequence similarity), stenotrophomonas humi r-32729(t) (97.1  %), stenotrophomonas nitritireducens l2(t) (96. ...201120400671
stenotrophomonas ginsengisoli sp. nov., isolated from a ginseng field.a gram-negative, non-spore-forming, rod-shaped bacterium, designated strain dcy01(t), was isolated from soil from a ginseng field in south korea and was characterized in order to determine its taxonomic position. 16s rrna gene sequence analysis revealed that strain dcy01(t) belonged to the gammaproteobacteria and was most closely related to stenotrophomonas koreensis kctc 12211(t) (98.4 % similarity), stenotrophomonas humi r-32729(t) (97.2 %), stenotrophomonas terrae r-32768 (97.1 %), stenotroph ...201019684314
conversion of linoleic acid into 10-hydroxy-12(z)-octadecenoic acid by whole cells of stenotrophomonas nitritireducens.the conversion of linoleic acid into 10-hydroxy-12(z)-octadecenoic acid by whole cells of stenotrophomonas nitritireducens as an isolated bacterium was optimized, and the optimal temperature, ph, and cell and substrate concentrations were 30 degrees c, 7.5, and 20 and 20 g/l, respectively. under these conditions, whole cells in a bioreactor produced 15 g/l 10-hydroxy-12(z)-octadecenoic acid in 2 h of reaction time without detectable byproducts. using 2 g/l linoleic acid, the cells produced 1.92 ...200818197675
isotopic analysis of n and o in nitrite and nitrate by sequential selective bacterial reduction to n2o.nitrite is an important intermediate species in the biogeochemical cycling of nitrogen, but its role in natural aquatic systems is poorly understood. isotopic data can be used to study the sources and transformations of no2- in the environment, but methods for independent isotopic analyses of no2- in the presence of other n species are still new and evolving. this study demonstrates that isotopic analyses of n and o in no2- can be done by treating whole freshwater or saltwater samples with the d ...200717580983
degradation of low-ethoxylated nonylphenols by a stenotrophomonas strain and development of new phylogenetic probes for stenotrophomonas spp. detection.an aerobic bacterium (bcc6), isolated from nonylphenol polyethoxylates (npeos)-contaminated sludge, was shown to be capable of degrading low-ethoxylated npeo mixtures. sequencing of 16s rrna gene (rdna) showed that it clustered with stenotrophomonas nitritireducens. fluorescent in situ hybridization (fish), performed on bcc6 strain and on the previously isolated stenotrophomonas bcal2, also involved in npeo degradation but clustering with s. maltophilia, showed that strain bcc6 did not hybridize ...200616392004
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