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effect of alkali on the structure of cell envelopes of chlamydia psittaci elementary bodies.suspensions of isolated cell envelopes of infectious elementary bodies (eb) of chlamydia psittaci at alkaline ph showed a rapid, extensive decrease in absorbance, accompanied by the release of a cell envelope component in a sedimentable form. this phenomenon was observed both at 0 c and with envelopes which had been previously heated to 100 c. monovalent and divalent cations effectively inhibited the turbidity loss, whereas ethylenediaminetetraacetate (edta) caused an accelerated decrease in tur ...19761375
protein-carbohydrate-lipid complex isolated from the cell envelopes of chlamydia psittaci in alkaline buffer and ethylenediaminetetraacetate.exposure of isolated cell envelopes from purified infectious elementary (eb) of chlamydia psittaci to sodium carbonate-bicarbonate buffer at ph 10 plus ethylenediaminetetraacetate (edta) results in partial solubilization of the total protein. the released materials represent 20% of the dry weight, 16% of the total protein, 40% of the total carbohydrate, and 9% of the total lipid of the cell envelopes. sucrose density gradient centrifugation, and sephadex g-200, sepharose 4b, or diethylaminoethyl ...19761376
ultrastructural studies of the nucleoids of the pleomorphic forms of chlamydia psittaci 6bc: a comparison with bacteria.the nucleoids of the various pleomorphic forms of chlamydia psittaci have been examined by direct observation of infected cells and by observations on isolated particles. the fixation and staining methods used were the same as those routinely used for the examination of bacteria to facilitate the comparison of chlamydial fine structure with that of bacteria. the nucleoids of reticulate bodies were composed of fine fibrils which extended throughout these particles. the nucleoids of intermediate b ...197656212
enzyme-linked immunosorbent assay for chlamydial antibodies.an enzyme-linked immunosorbent assay (elisa) detected chlamydial antibodies in human sera. the assay antigen produced in cell cultures infected with chlamydia psittaci was formalin-fixed to microplates. single convalescent-phase sera positive for chlamydial antibodies by a complement-fixation test were positive at even higher dilutions by elisa. paired sera with diagnostic rises in complement-fixing antibody showed seroconversion by elisa also. control sera from persons with no history of chlamy ...197772759
fulminant psittacosis.two patients died of psittacosis after presenting with generalised toxaemia, acute renal failure, and evidence of pancreatitis. death was attributed to the virulence of the chlamydial strain and the delay in antemortem diagnosis. in one case chlamydia psittaci was isolated from necropsy lung tissue. a third case of psittacosis suggested person-to-person or fomite spread, which is rarely reported. infection was acquired from an apparently healthy, imported and quarantined cockatiel. import restri ...197985004
direct immunofluorescence tests with counterstains for detection of chlamydia psittaci in infected avian tissues.different methods of preparation and serological evaluation of rabbit globulins for use in fluorescent antibody conjugate and different methods of counterstaining with fluorescent antibody tests were evaluated for detection of chlamydia psittaci in infected turkey tissues. the agar gel precipitin reaction was that chosen for testing and selecting antiserums to be used for fluorescein isothiocyanate conjugation. the fluorescent antibody staining was most pronounced with conjugate made from globul ...197991417
chlamydia psittaci: growth characteristics and enumeration of serotypes 1 and 2 in cultured cells.the growth characteristics of chlamydia psittaci serotypes 1 and 2 (ovine and bovine origin) were studed in mouse l cells. formation of inclusions and yield of infectious progeny for serotype 1 were maximal when host cells were treated with cycloheximide and the ph in the cell culture medium was 7.2-7.4. the number of cells that contained inclusions and the infectivity yield for serotype 2 were maximal when the ph was 6.6-7.0. treatments with diethylaminoethyl dextran and cycloheximide increased ...197994339
[isolation and identification of clamydia psittaci as the pathogen in enzootic abortion of sheep in eastern slovakia].abortions in ewes occurred on a large scale in three localities in eastern slovakia. antibodies to the group-type ornithosis antigen were detected in titres of 1 : 128 to 1 : 2048 in the aborting ewes in the mentioned localities. suspensions were prepared from the four samples of material, obtained either from the placentae and afterbirths of the aborting ewes or from the tissues of the aborted foetuses. seven-day old yolk sacs of chicken embryos were infected with these suspensions. four strain ...197899861
comparison of direct and modified direct complement-fixation and agar-gel precipitin methods in detecting chlamydial antibody in wild birds.detection of chlamydial antibody in serums of wild birds was compared for the following methods: direct complement-fixation (dcf), modified dcf (mdcf), and agar-gel precipitin (agp). the birds species used were great-tailed grackles (cassidix mexicanus), common grackles (quiscalus quiscula), brown-headed cowbirds (molothrus ater), bronzed cowbirds (tangavius aeneus), and mourning doves (zenaida macroura). the birds were either inoculated with an isolate of chlamydia psittaci obtained originally ...1978100098
[aetiological studies on viral pneumonia (author's transl)].401 cases of viral pneumonia diagnosed between january 1973 and august 1975 were investigated serologically by the complement-fixation test. the percentage distribution of the responsible pathogenic organism in this series of cases was as follows: influenza virus a 45.9%, mycoplasma pneumoniae 19.5%, coxsackie b viruses 9.2%, cytomegalovirus 7.5% and chlamydia psittaci 8.5%. the remaining 9.4% cases were caused by adeno, parainfluenza, measles, influenza b, herpes simplex and respiratory syncyti ...1976185813
[atypical pneumonia, etiology and possibilities for the diagnosis (author's transl)].beginning with the antimicrobial chemotherapy a decrease in the incidence of bacterial pneumonias is accompanied by a relative increase in the incidence of the so-called atypical pneumonia. this disease syndrome is predominantly caused by mycoplasma pneumoniae, chlamydia psittaci, coxiella burneti and various viruses. in addition, bacteria which are usually involved in lobar pneumonia may occasionally cause atypical pneumonias. the present publication is concerned with the most frequently occurr ...1976186398
growth and effect of chlamydiae in human and bovine oviduct organ cultures. 1979223717
modification of the microimmunofluorescence test to provide a routine serodiagnostic test for chlamydial infection.a modification of the microimmunofluorescence test to provide a practicable routine serodiagnostic test for detecting and characterising chlamydial infection is described which uses four antigen pools, one of which corresponds with each of the four main clinical and epidemiological types of chlamydial infection. the three subgroup a chlamydia (chlamydia trachomatis) pools are: pool 1, hyperendemic trachoma tric agent serotypes a, b, and c; pool 2, paratrachoma tric agent serotypes d, e, f, g, h, ...1977326816
[plaque assay for chlamydia psittaci in tissue samples (author's transl)].direct isolation of chlamydia psittaci (var, ovis) from mouse spleen was performed in parallel by plaque assay on mccoy cells and by staining of cytoplasmic inclusions in hela 229 cells. there was a significant difference between the frequency of isolation: the plaque technique was highly sensitive and reproducible; it offers a potential tool for investigations requiring the accurate quantitation of chlamydiae in tissue sample.1977334017
parasite-specified phagocytosis of chlamydia psittaci and chlamydia trachomatis by l and hela cells.phagocytosis of the 6bc strain of chlamydia psittaci and the lymphogranuloma venereum 440l strain of chlamydia trachomatis by l cells and hela 229 cells occurred at rates and to extents that were 10 to 100 times greater than those observed for the phagocytosis of escherichia coli and polystyrene latex spheres. both species of chlamydia were efficiently taken up by host cells of a type they had not previously encountered. phagocytosis of chlamydiae was brought about by the interaction of parasite ...1978344217
legionnaires' disease: antigenic peculiarities, strain differences, and antibiotic sensitivities of the agent.paired sera from victims of legionnaires' disease showed, in many cases, significant rises in immunoglobulin g antibodies to both the causative agent (la) of legionnaires' disease and chlamydia psittaci, but concurrent rises in immunoglobulin m antibodies only against la. guinea pigs experimentally infected with la likewise responded with antibodies to both c. psittaci and la. guinea pigs infected with la also reflected significant differences in antigenic makeup and in pathogenicity among four ...1978355583
sensitivity of immunoperoxidase and immunofluorescence staining for detecting chlamydia in conjunctival scrapings and in cell culture.the sensitivities of giemsa, immunofluorescence, and immunoperoxidase staining for the detection of chlamydia psittaci inclusions in conjunctival scrapings and in irradiated mccoy cell monolayers were compared. conjunctival specimens were obtained from a cat colony in which a trachoma-like disease, feline chlamydial keratoconjunctivitis, was endemic. the two immunochemical techniques were found to be of equal sensitivity and 50% to 100% more sensitive than giemsa stain. permanent preparations of ...1978368085
kinetics of the complement fixing and immunofluorescent antibody response in experimental chlamydiosis in ewes.normal, 70 days pregnant ewes were inoculated i.v. with a chicken embryo grown chlamydia psittaci strain. the ewes presented a biphasic febrile curve and aborted 30 to 68 days after inoculation. chlamydiae were isolated from aborted fetuses and from vaginal swabs. ewes developed cf and if antibodies titrating 1:320 to 1:1024 two to three weeks after inoculation. a second antibody rise occurred one to two weeks before or after abortion and reached titers of 1:640 to 1:1024. immunoglobulin fractio ...1978373577
target tissues associated with genital infection of female guinea pigs by the chlamydial agent of guinea pig inclusion conjunctivitis.female guinea pigs were experimentally infected in the genital tract with the strain of chlamydia psittaci that causes guinea pig inclusion conjunctivitis. chlamydiae were found frequently in superficial squamous epithelial cells of the exocervix, along with heavy involvement at the squamocolumnar junction. in this zone chlamydiae were observed in columnar cells, but inflammation and chlamydial inclusions were not observed in true endocervical epithelium. these observations were supported by res ...1979374637
prevalence of certain antibodies to selected disease-causing agents in wild turkeys in texas.in texas in 1976 and 1977, rio grande turkeys (meleagris gallopavo intermedia) from seven counties and eastern turkeys (m. g. silvestris) from one county were tested for antibodies to selected poultry pathogens. standardized serological tests disclosed reactors to salmonella pullorum (2.4%), s. typhimurium (2.3%), and mycoplasma gallisepticum (4.8%). there were no reactors to newcastle disease virus or chlamydia psittaci. prevalence of m. gallisepticum antibody in wild turkeys was significantly ...1979384989
diagnostic specificity of immunoglobulin m (igm) response in differentiation legionnaires' disease from psittacosis.specific igm and igg antibody responses to legionella pneumophila (ldb) and chlamydia psittaci (psi) in serum specimens from 22 cases of legionnaires' disease (ld) were examined by micro-immunofluorescence (if) tests to explore the diagnostic significance of the igm antibody response. serial samples from 5 patients with ld showed greater than or equal to 4-fold changes in igg antibody against ldb and psi. all 5 patients possessed igm antibodies against ldb but not against psi. in single convales ...1979394307
isolation of chlamydia psittaci ovis in sheep thyroid cell culture. 1979433110
arrays of hemispheric surface projections on chlamydia psittaci and chlamydia trachomatis observed by scanning electron microscopy.scanning microscopy of two strains of chlamydia psittaci and four strains of chlamydia trachomatis representative of the wide diversity in origin and behavior of members of the genus revealed patches of regular arrays of hemispheric projections on the surfaces of elementary bodies of all six strains. these distinctive and perhaps unique surface structure are probably present in all populations of chlamydiae.1979438131
biotyping of chlamydia psittaci based on inclusion morphology and response to diethylaminoethyl-dextran and cycloheximide.strains of chlamydia psittaci from cattle, sheep, pigs, mice, guinea pigs, rabbits, cats, and parrots were subdivided based on their biological characteristics. chlamydiae grown in the yolk sac of chicken embryos were used to infect l cell monolayers. the host cells were infected without further treatment or treated with diethylaminoethyl-dextran, cycloheximide, or both. the following criteria were used for biotyping the strains: the morphology of the inclusions and time after infection at which ...1979457272
interaction of chlamydia psittaci reticulate bodies with mouse peritoneal macrophages.noninfectious reticulate bodies of chlamydia psittaci are readily phagocytized by thioglycolate-elicited mouse peritoneal macrophages in monolayer culture. the internalized reticulate bodies are rapidly destroyed as indicated by a 60 to 70% decrease in trichloroacetic acid-precipitable radioisotopic counts in the macrophage pellet by 10 h and a concomitant increase of the trichloroacetic acid-soluble radiolabeled chlamydial nucleic acid in the cytoplasm. this intracellular destruction of reticul ...1979468374
changes in the ultrastructure of chlamydia psittaci produced by treatment of the host cell with deae-dextran and cycloheximide. 1979469985
stimulation of peroxidase by chlamydial infection: cytochemistry of guinea pig conjunctival epithelium.guinea pig inclusion conjunctivitis, a naturally occurring chlamydial disease of guinea pigs, resolves spontaneously after 3 to 4 weeks. the factors responsible for curbing the infection have not yet been specifically defined. since iwata (invest. ophthalmol. 15:297-301, 1976) reported cytochemical activity for peroxidase in the conjunctival epithelium of the normal rat, we undertook these studies to determine whether a similar activity exists in the guinea pig, and if so, whether it functions i ...1979478644
cytochalasin b does not inhibit ingestion of chlamydia psittaci by mouse fibroblasts (l cells) and mouse peritoneal macrophages.cytochalasin b did not inhibit ingestion of chlamydia psittaci by either mouse fibroblasts (l cells) or mouse peritoneal macrophages in concentrations that produced distinctive morphological changes and inhibited phagocytosis of polystyrene latex beads and escherichia coli k-12.1979478646
use of hela cell guanine nucleotides by chlamydia psittaci.exogenous guanine was found to be incorporated into the nucleic acids of chlamydia psittaci when the parasite was grown in hela cells containing hypoxanthine guanine phosphoribosyltransferase (ec 2.4.2.8) activity but not when the parasite was grown in transferase-deficient hela cells. no evidence for a chlamydia-specific transferase activity was found in either transferase-containing or transferase-deficient infected hela cells. it is concluded that c. psittaci is incapable of metabolizing guan ...1979478649
wheat germ agglutinin blockage of chlamydial attachment sites: antagonism by n-acetyl-d-glucosamine.addition of 2 to 10 micrograms of wheat germ agglutinin (wga), a lectin from triticum vulgaris specific for n-acetyl-d-glucosamine, per ml to suspensions of mouse fibroblasts (l cells) blocked the attachment of 14c-labeled chlamydia psittaci 6bc to the l-cell surface. wga and strain 6bc competed for similar sites on l cells, but once bound, one was not replaced by the other. n-acetyl-d-glucosamine, but not other monosaccharides of related structure, antagonized the blocking action of wga. lectin ...1979500195
infective endocarditis with glomerulonephritis associated with cat chlamydia (c. psittaci) infection.a patient with glomerulonephritis and endocarditis is described who had evidence of feline chlamydia psittaci infection. treatment with antichlamydial drugs resulted in resolution of the glomerulonephritis and the endocarditis. it is recommended that screening for chlamydia is included in the investigation of patients with suspected or obscure endocarditis.1979508458
isolation of a chlamydial agent from rocky mountain bighorn sheep.a total of 53 clinical specimens from both healthy and diseased rocky mountain bighorn sheep (ovis canadensis) were examined for chlamydia. an agent consistently lethal for chicken embryos was recovered from a nasal swab taken from a normal ewe. this agent, designated bhs-15, possesses antigens which fix complement in the presence of anti-chlamydial serum, is susceptible to chlortetracycline, and is resistant to sodium sulfadiazine and streptomycin. attempts to culture the isolate in quality con ...1979522218
experimental pneumonia in red deer (cervus elaphus l) produced by an ovine chlamydia.an isolate of chlamydia psittaci from ovine pneumonia produced extensive pneumonia in red deer after endobronchial inoculation. associated clinical signs lasting for several days included pyrexia, inappetance, increased pulse and respiration rate, and physical distress after handling, but no coughing or upper respiratory symptoms. histologically, an acute exudative reaction was present after two days, and an early proliferative response after seven days. both the clinical and pathological respon ...1979532073
experimental transmission of chlamydia psittaci to turkeys from wild birds.wild birds were inoculated with chlamydia psittaci to determine species that could be potential hosts and vectors in transmitting the agent to domestic turkeys. infection occurred in turkeys exposed to starlings (sturnus vulgaris), common grackles (quiscalus quiscula), brown-headed cowbirds (molothrus ater), and inca doves (cardafella inca). mourning doves (zenaidura macroura) shed the agent sparingly, but turkeys exposed to them did not become infected, these findings and knowledge of the habit ...1979546413
[the replication cycle of chlamydia trachomatis and chlamydia psittaci: ultrastructural analysis].an ultrastructural comparative analysis is reported on the replication cycle of six strains of chlamydia trachomatis, recently isolated, and chlamydia psittaci strain 6bc, grown in cell cultures. important morphological distinctions of the bacterial walls are documented. in chlamydia trachomatis the cell wall seems more rigid. this causes, during the morphogenesis of the elementary body, its separation from the cell membrane with formation of an electron transparent space, which is not demonstra ...1979553521
[antibodies against chlamydia trachomatis in patients with non-gonococcal urethritis and in the healthy italian population].the authors report the results of a study on the prevalence of serum antibodies against chlamydia trachomatis among patients with non-gonococcal urethritis caused by chlamydia trachomatis and diagnosed on the basis of the isolation of the microorganism in cell cultures, among patients with non-gonococcal urethritis of unknown etiology and in groups of healthy population, in italy. the search for antibodies was performed both with complement-fixation tests in micro-titer system, using an antigen ...1979553522
sucrose density differences of chlamydia psittaci 6bc in relation to its host.previous studies on chlamydia psittaci 6bc propagated in different hosts have shown differences in cytotoxicity but no differences in the ultrastructure of the individual particles. it is shown here that the 6bc strain derived from yolk sac of infected chick embryo sedimented in sucrose gradients at lower densities than the 6bc strain derived from l-cells. host-related modifications of lipid concentrations of the 6bc strains have been previously documented by others. it is thought that the pheno ...1977559537
growth of chlamydia psittaci in macrophages.survival and growth of l-cell-cultivated chlamydia psittaci occurred in mouse macrophages in vitro. two major factors governing the intracellular fate of chlamydiae in macrophages are: (i) the multiplicity of infection (moi), i.e., the elementary body (eb)-to-macrophage ratio, and (ii) the state of the eb. at a low moi (1:1) survival and growth of live, untreated chlamydiae were optimal. the chlamydiae were internalized in macrophages within 30 to 40 min. eb proceeded to differentiate into retic ...1978565338
interaction of chlamydia psittaci with mouse peritoneal macrophages.l-cell-grown chlamydia psittaci elementary bodies (eb) were rapidly phagocytized by mouse peritoneal macrophages in vitro. however, the intracellular fate of chlamydiae in macrophages appeared to be dependent on the multiplicity of infection (moi), i.e., the eb-to-macrophage ratio, and the treatment of the eb. at an moi of 1:1 or less, survival is maximal, and growth and multiplication of live, untreated chlamydiae did occur. in contrast, at a high moi (100:1), survival of chlamydiae is reduced, ...1978565339
[chlamydia psittaci infections among hospital personnel]. 1977607278
division of single host cells after infection with chlamydiae.mouse fibroblasts (l cells) were infected in suspension with chlamydia psittaci (6bc) and then plated out on a solid substrate at a density of 80 cells per cm2 so that the effect of chlamydial infection on the division of single host cells and their progeny could be determined. uninfected l cells multiplied with a mean generation time of 15 h. the generation time of single l cells infected with 1.5 50% infectious units (id50) of c. psittaci was over twice as long. half of the infected l cells ha ...1978624590
chlamydia psittaci infection of horses with respiratory disease.two strains of chlamydia psittaci were isolated from the nasal tract of horses with acute respiratory disease. these 2 isolates (ns 121 and ns 172) were characterized as chlamydia on the basis of their morphology, tinctorial property, growth in chicken embryos, inability to grow on bacterial media and their possession of chlamydial common complement fixing group antigen. they were identified as c. psittaci on the basis of resistance to sodium sulphadiazine. the present strains were not pathogeni ...1978631105
kinetics of phagocytosis of chlamydia psittaci by mouse fibroblasts (l cells): separation of the attachment and ingestion stages.the kinetics of phagocytosis of chlamydia psittaci (6bc) by monolayers of mouse fibroblasts (l cells) was studied with an assay that distinguished between the attachment and ingestion phases of phagocytosis. at multiplicities of 10 and 100 50% infectious doses (id50) per l cell, virtually all of the inoculated c. psittaci had been attached and ingested after 60 min at 37 degrees c. at multiplicities of 500 to 5,000 id50 per l cell, the initial rates of attachment and ingestion of c. psittaci to ...1978631892
loss of inorganic ions from host cells infected with chlamydia psittaci.mouse fibroblasts (l cells) infected with the 6bc strain of chlamydia psittaci released potassium ion (k(+)) into the extracellular milieu in a way that depended on size of inoculum and time after infection. when the multiplicity of infection was 500 to 1,000 50% infectious units (id(50)) per l cell, loss of intracellular k(+) was first apparent 4 to 10 h after infection and was nearly complete at 6 to 20 h. magnesium ion and inorganic phosphate (p(i)) were also released. similar multiplicities ...1978640730
surface projections and internal structure of chlamydia psittaci.the outermost surface of the small infectious forms of chlamydia psittaci contain geometrically arranged spikes distributed over approximately 50% of the bacterial surface. the spikes are located opposite the concave side of an electron-dense crescent-shaped chlamydial core.1978641015
experimentally induced feline chlamydial infection (feline pneumonitis).cats exposed to aerosols of feline chlamydia psittaci developed a disease characterized principally by conjunctivitis. signs of conjunctivitis appeared between postexposure days (ped) 5 and 10, were often unilateral initially, and persisted for 22 to 45 days. fever followed the onset of conjunctivitis (ped 11 to 15) and persisted for 3 to 8 days. signs of mild rhinitis (occasional sneezing and mild serous nasal discharge) occurred in some cats between ped 8 and 37. neither signs of lower respira ...1978646190
immune adherence hemagglutination: alternative to complement-fixation serology.immune adherence hemagglutination (iaha) was compared to complement fixation (cf), using standard procedures, for serological testing of human sera with a number of commercially available antigens. the antigens included herpes simplex, measles, cytomegalo-, and influenza (type b) viruses, as well as mycoplasma pneumoniae and chlamydia psittaci (chlamydia group). the iaha test was found to be as specific as the cf test, but 4 to 20 times as sensitive with all antigens tested. antigen titers were ...1978649762
the isolation of a chlamydia psittaci-like agent from a free-living african buffalo (syncerus caffer). 1978676013
mechanisms of infertility in genital tract infections due to chlamydia psittaci transmitted through contaminated semen.ten heifers inseminated with semen artificially contaminated with chlamydia psittaci were not pregnant 40 days later, whereas five of 10 control heifers inseminated with the same semen mixed with control diluent became pregnant. normal embryos were recovered two and one-half or three days after insemination of another group of animals with semen containing c. psittaci, a finding indicating that fertilization failure was not responsible for the infertility. uterine biopsy samples taken from two h ...1978681792
[human chlamydia psittaci infection]. 1978705936
isolation of chlamydia psittaci from a patient with interstitial keratitis and uveitis associated with otological and cardiovascular lesions.a case history of a 15-year-old schoolgirl with fluctuating bilateral uveitis, bilateral stromal keratitis with vascularisation, and bilateral deafness associated with tinnitus and balance disturbance is described. three years from the onset of her clinical signs she died of a sudden cardiac arrest caused by endocarditis associated with valvular and arterial lesions. chlamydia psittaci was isolated from her conjunctiva. in her blood type-specific antichlamydial antibody at a level of 1/64 agains ...1978708673
chlamydia psittaci infection in danish cattle.intestinal tract infection by chlamydia psittaci was demonstrated in one cattle herd by isolation from faecal specimens, using embryonated eggs. such infections were observed in all animals younger than 12 months, in 60% of the heifers and in none of the adult cows. the presence of infection correlated (r=0.511) with the serum titre of compliment fixation antibodies against chlamydial antigen. young calves, which were spontaneously infected with chlamydia postnatally, developed ileitis and moder ...1978716920
response of c3h/hej and c3h/hen mice and their peritoneal macrophages to the toxicity of chlamydia psittaci elementary bodies.intravenous injection of toxic doses of chlamydia psittaci elementary bodies into endotoxin-responsive c3h/hen mice or endotoxin-nonresponsive c3h/hej mice resulted in essentially identical time intervals to death. inoculation of monolayer cultures of thioglycolate-stimulated peritoneal macrophages from the two strains of mice with 250 elementary bodies per macrophage resulted in immediate host cell toxicity, although the c3h/hej macrophages were somewhat less sensitive to elementary body toxici ...1978730377
use of enteric vaccines in protection against chlamydial infections of the genital tract and the eye of guinea pigs.guinea pigs in a test group were fed living guinea pig inclusion conjunctivitis (gpic) organisms classified as chlamydia psittaci in 60% yolk-sac suspensions as enteric vaccines, while animals in a control group received uninfected yolk sac. seven test animals and 14 control animals were challenged 11 or 22 days later with 1,000 50% infectious doses of gpic organisms in either the conjunctiva or the vagina. evidence of protection from mucosal infection in both sites was noted in test animals. cl ...1978739153
chlamydia shedding by four species of wild birds.four wild bird species--great-tailed grackle (cassidix mexicanus), common grackle (quiscalus quiscula), brown-headed cowbird (molothrus ater), and mourning dove (zenaidura macroura)--were either inoculated intratracheally with chlamydia psittaci or exposed indirectly as uninoculated cagemates. shedding of chlamydiae was monitored by inoculating mice with suspensions of material eluted from cloacal swabs collected from all birds, usually at 3-day intervals. sporadic shedding of chlamydiae was dem ...1978749892
the incidence and aetiology of respiratory tract infections in general practice--with emphasis on mycoplasma pneumoniae.the incidence of respiratory tract infections in patients seeking medical advice at a community care centre (dalby) during 1973 and 1974 was studied. about every third patient seen at this primary health station presented with signs of such infections. in the age groups less than 10, 10-19, 20-39, 40-59 and greater than or equal to 60 years, respiratory tract infections accounted for 65, 45, 32, 18 and 9% of the fotal number of diagnoses made during 1974. the aetiology of acute respiratory tract ...1976783048
observations of the surface projections of infectious small cell of chlamydia psittaci in thin sections. 1976799722
vaccination against feline pneumonitis.a commercially available modified live chlamydial vaccine against feline pneumonitis was tested in 26 cats for its ability to protect against aerosol challenge exposure to the feline pneumonitis strain of chlamydia psittaci. after cats were challenge exposed (30 days after vaccination), pyrexia of greater than 40.0 c occurred in 81% of nonvaccinated (control) cats and in 13% of vaccinated cats (principals). evidence of upper respiratory tract disease and the presence of the agent in ocular fluid ...1977921030
toxicity of low and moderate multiplicities of chlamydia psittaci for mouse fibroblasts (l cells).when mouse fibroblasts (l cells) were infected in suspension or in monolayer with 10 to 100 50% infectious doses (id(50)) of chlamydia psittaci (6bc) per host cell, they showed signs of damage 24 to 48 h later. host-cell injuries were termed multiplication dependent when both the ingestion and subsequent reproduction of c. psittaci were required; when only ingestion but not replication was needed, the injuries were considered to be multiplication independent. the time that the injury was first a ...1977924681
experimental chlamydiosis in wild and domestic lagomorphs.chlamydia psittaci (strain m56, the agent of epizootic chlamydiosis of muskrats and hares) was highly lethal for the snowshoe hare (lepus americans) following intravenous inoculation, whereas the agent was much less virulent for cottontail (sylvilagus floridanus) and albino domestic rabbits (oryctolagus cuniculus). tissue titres of strain m56 were generally higher after 96 hr in the snowshoe hare than in tissues of the other lagomorphs. spleen, liver and bone marrow were apparently the chief sit ...1976933312
utilization of exogenous thymidine by chlamydia psittaci growing in the thymidine kinase-containing and thymidine kinase-deficient l cells.the incorporation of [3h]thymidine into the deoxyribonucleic acid (dna) of chlamydia psittaci (strain 6bc) growing in thymidine kinase (adenosine 5'-triphosphate-thymidine 5'-phosphotransferase, ec 1.7.1.21)-containing l cells, l(tk+), and thymidine kinase-deficient l cells, lm(tk-), was examined by autoradiography. label was detected over c. psittaci inclusions in l(tk+) but not lm(tk-) cells. no evidence for a chlamydia-specific thymidine kinase activity in either l(tk+) or lm(tk-) cells was o ...1976942715
autoradiography of [3h]thymidine-labeled chlamydia psittaci 6bc in mononuclear phagocytes.incorporation of tritiated [3h]thymidine by chlamydia psittaci 6bc was achieved by growing the parasites in chick embryo yolk sac explants which were exposed to exogenous labeled thymidine. these labeled, purified chlamydiae were next observed by autoradiography within mouse peritoneal macrophages. the number of silver grains remained constant in the cytoplasm of macrophages throughout the developmental cycle of the parasite. the proliferation of labeled chlamydiae in macrophages was confirmed b ...1976943214
alterations in the ultrastructure of chlamydia psittaci 6bc harvested from the allantoic fluid of chick embryos.the allantoic fluid of chick embryos infected with chlamydia psittaci is routinely used as a source of material for the study of the chemical and biological properties of the chlamydiae. we have examined pellets recovered from this allantoic fluid by low- and high-speed centrifugation, as well as high-speed pellets which had been stored at -70 degrees c, and we find that all of the pleomorphic forms of the chlamydiae are present in these materials. the reticulate bodies and large intermediate bo ...1976943217
characterization of miniature pig kidney cells and their resistance to chlamydial infection.a miniature pig kidney cell line has been established from porcine fetuses taken aseptically by hysterectomy and maintained for more than 50 passages in eagle's minimal essential medium containing 10% heat-inactivated newborn calf serum. cell transfers were performed each week. primary and serially passaged cells were found to be highly refractory to infection by chlamydia trachomatis strains tw-3, bour, and lgv 440l and chlamydia psittaci strains meningopneumonitis and 6bc and insusceptible to ...1976944002
detection of dna polymerase activity in chlamydia psittaci.dna polymerase activities of intact and disrupted suspensions of the mature infectious, extracellular elementary bodies of the meningopneumonitis strain of chlamydia psittaci were studied. intact elementary bodies failed to incorporate labeled thymidine triphosphate (ttp), but homogenates of the organisms did incorporate ttp into the acid insoluble fraction; the reaction continued at a linear rate for 60 min and the newly synthesized dna hybridized exclusively with dna derived from c. psittaci e ...1976957524
differences in physicochemical and antigenic properties of chlamydial strains.antigenic analysis of chlamydia psittaci, c. trachomatis, and lymphogranuloma venereum (lgv) revealed basic physicochemical differences among the three chlamydial strains. these were manifested in structural, isoelectric points, absorption spectra, and in the characteristics of the chromophobe-containing proteins. the effective period of sonication for c. psittaci and c. trachomatis is around 60 min during which the linkages most susceptible to external sonication forces were broken, releasing a ...1976963615
requirements for ingestion of chlamydia psittaci by mouse fibroblasts (l cells).ingestion of 14c-amino acid-labeled chlamydia psittaci (6bc) by mouse fibroblasts (l cells) was inhibited when the host cells were incubated for 30 min at 37 degrees c in earle salts containing 10 mug of crystalline trypsin per ml. tryptic digestion also inhibited the ingestion of 1-mum polystrene latex beads. trypsin-treated l cells almost completely recovered their ability to ingest chlamydiae after 4 h at 37 degrees c in medium 199 with 5% fetal calf serum. cycloheximide (10 mug/ml) blocked t ...1976965090
immediate toxicity of high multiplicities of chlamydia psittaci for mouse fibroblasts (l cells).one hour after suspensions of mouse fibroblasts (l cells) were exposed to 500 to 1,000 l-cell 50% infectious doses of chlamydia psittaci (6bc), the l cells failed to attach to and spread out on solid substrates, phagocytosed polystyrene latex spheres at reduced rates, incorporated less [14c]isoleucine into protein, and had smaller soluble pools of nucleoside triphosphates. the infected l cells began to die at 8 h and were all dead by 20 h. lower multiplicities of infection took correspondingly l ...1976985806
phagocytic and chlamydiae-inhibiting activities of stimulated and nonstimulated periotneal mouse macrophages.phagocytic and chamydiacidal properties of nonstimulated and stimulated mouse mononuclear cells for two chlamydia psittaci 6bc strains were investigated. it was determined that macrophages kept in monolayer culture (i.e. stimulated phagocytes) developed much more efficient chlamydiacidal ability than did cells kept in suspension directly after harvest (i.e. nonstimulated phagocytes). a thousandfold decrease of chlamydial infectivity was observed 60 min after induction of phagocytosis in stimulat ...1976986871
observations on the involvement of wildlife in an epornitic of chlamydiosis in domestic turkeys.in an investigation of potential wildlife reservoirs of chlamydia psittaci at the site of an acute, highly fatal epornitic of chlamydiosis in domestic turkeys in texas, various species of wild birds and rodents were captured and tested for chlamydial serum antibodies and chlamydiae in their tissues. thirteen (65%) of 20 blackbirds (agelaius sp), 4 (44%) of 9 killdeer (oxyechus vociferus), 3 (27%) of 11 sparrows (passer sp) and 1 of 4 mourning doves (zenaidura macroura) were seropositive, but chl ...1976988009
some physical properties of a sheep pneumonia strain of chlamydia psittaci. 1976997192
lysosomes and the "toxicity" of rickettsias. vi. in vivo response of mouse peritoneal phagocytes to l-cell-grown chlamydia psittaci 6bc strain.the l-cell-grown 6bc strain of c. psittaci inoculated intraperitoneally in mice induced an injurious effect on mononuclear phagocytes and their lysosomes; the influx of polymorphonuclear phagocytes (pmn's) increased markedly and the pmn's showed karyorrhexis and lysis. cytochemical methods failed to detect chlamydial forms in peritoneal fluids from day 1 and up to 6 days after inoculation of mice. chlamydial infectivity was not detected in either the cell-bound or the cell-free fractions of peri ...19751090349
competition between chlamydia psittaci and l cells for host isoleucine pools: a limiting factor in chlamydial multiplication.l cells (mouse fibroblasts) supported the multiplication of the obligately intracellular parasitic bacterium chlamydia psittaci (strain 6bc) when incubated in fresh growth medium (medium 199 + 5% fetal calf serum). when incubated in the medium supernatant from a 24-h-old culture of uninfected l cells (24-h used medium), uninfected cells did not divide and infected cells did not provide an adequate environment for the multiplication of c. psittaci, which persisted in a noninfectious latent state ...19751095493
immunofluorescence of peritoneal phagocytes after infection of mice with l-cell-attenuated chlamydia psittaci 6bc.large amounts of particulate antigen of chlamydia psittaci 6bc attenuated by growth in l cells were phagocytized by peritoneal mononuclear phagocytes during the 1st h after intraperitoneal inoculation. the phagocytes subsequently destroyed the immunofluorescent (if) properties of the chlamydial antigens. it is suggested that the early damage of phagocytes by lysosomal enzymes activation induced by chlamydiae contributed to the relatively early disappearance of if antigens from the peritoneal flu ...19751097065
an epornitic of fatal chlamydiosis (ornithosis) in south carolina turkeys.an unusual epornitic of fatal chlamydiosis occurred in a flock of 10,283 domestic turkeys in south carolina. total mortality over a 2-week period was 483 birds (4.7% of the flock). the principal gross lesion was severe pericarditis, but there was little or no airsacculitis, an observation at variance with many previous reports of chlamydiosis. furthermore, an unusually heavy infestation of the turkeys with sanguivorous black flies (simulium slossonae and s congareenarum) was observed at the time ...19751112744
protein profiles of dense-centered forms of five chlamydial strains of animal origin.purified dense-centered form of 1 bovine strain (lw613) and 3 ovine strains (b577, 034-eye, and 047-eye) of chlamydia psittaci and 1 murine strain of chlamydia trachomatis (mopn) were dissociated in the presence of sodium dodecyl sulfate (sds) and 2-mercaptoethanol. the number of polypeptides detected in the 5 strains varied between 17 and 20, with a molecular weight range of 29,000 to 120,000. two polypeptides predominated and comprised approximately a third of the total protein in each of the ...19751147351
electron microscopy of the in vivo internalization of virulent chlamydia psittaci 6bc strain.the internalization of virulent chlamydia psittaci 6bc particles by wandering mononuclear phagocytes in the peritoneal cavity of intraperitoneally inoculated mice occurred asynchronously, i.e., fragile reticulate bodies (rb) appeared to be more readily phagocytized than the rigid elementary bodies (eb). early damage of mononuclear phagocytes occurred after internalization of chlamydiae. this was followed by a decreased uptake of particles, and may explain the relatively long persistence (up to ...19751148946
utilization of l-cell nucleoside triphosphates by chlamydia psittaci for ribonucleic acid synthesis.long-term, 32-p-labeled l cells were infected with the obligately intracellular parasite chlamydia psittaci (strain 6 bc). at 20 h postinfection, [3-h]uridine was added, and the infected cells were sampled at intervals for incorporation of the labels into the uridine triphosphate (utp) and cytidine triphosphate (ctp) pools of the host l cell and the uridine monophosphate (ump) and cytidine monophosphate (cmp) in 16s ribosomal ribonucleic acid (rna) of the parasite. the specific activity of the n ...19751168632
ultrastructural cytochemical evidence for the activation of lysosomes in the cytocidal effect of chlamydia psittaci.the cytopathic effect of the polyarthritis strain of chlamydia psittaci was studied in cultured bovine fetal spleen cells and found to be mediated by the release of lysosomal enzymes into the host cytoplasm during the late stages of chlamydial development. ultrastructural cytochemical analysis and cell fractionation studies of infected cells revealed a close relationship between the stage of chlamydial development, fine structural features of the host, and localization of lysosomal enzyme activi ...19751172486
insidious endocarditis caused by chlamydia psittaci. 19751212580
[experimental infection of guinea pigs with chlamydia. 1. pathomorphological studies].the changes that occurred following nasal instillation of a bovine strain of chlamydia psittaci were characterized by hypertrophy and proliferation of epithelial cells and exudates containing polymorphonuclear leukocytes, with acute bronchopneumonia and less intense inflammatory reactions in nasal and tracheal mucosa, spleen and pulmonary lymph nodes. the intestines, liver, kidney and brain were scarcely affected. there was little prospect of complete recovery from the clinically mild, or even s ...19751227428
[morphological ultrastructural and immunological studies of a rickettsia isolated from a parrot (author's transl)].a rickettsia isolated from a parrot was found by use of electron microscopic and serological technic to be a coxiella burneti. in cell culture one can see a dramatic polymorphism and a gram negative-like structure of the cell wall. they divide by binary fission with no appearance of a complexe cycle of development like the one described for chlamydia psittaci. many myelinic fibrils and altered coxiella suggest that the host cell react very deaply.19751238044
ultrastructural studies of chlamydia psittaci 6bc in situ in yolk sac explants and l cells: a comparison with gram-negative bacteria.chlamydia psittaci (6bc) was grown in yolk sac explants and in l cells and fixed by perfusion in situ to provide undamaged material for comparison with gram-negative bacteria. reticulate, intermediate, and elementary bodies were all seen to lack a well-defined periplasmic space; intermediate and elementary bodies showed condensations of the nucleoid which differ from common bacterial configurations; and the cytoplasm of highly condensed elementary bodies was much more electron dense than that of ...19751238156
the effect of purification on the ultrastructure and infectivity of egg-attenuated chlamydia psittaci (6bc).a procedure is described for the purification of mixed populations of the three different morphological forms of chlamydia psittaci (6bc) from infected yolk sac membranes. elementary bodies and small intermediate bodies are not perceptibly damaged during purification which involves homogenization of the host cells, differential centrifugation, sedimentation through 20% sucrose, and treatment with trypsin. the observation that elementary bodies undergo plasmolysis in 20% sucrose is interpreted as ...19751238157
detection of igm antibodies to chlamydia trachomatis, chlamydia pneumoniae, and chlamydia psittaci from japanese infants and children with pneumonia.chlamydia trachomatis (c. trachomatis) is now well established as a pathogen of neonatal inclusion conjunctivitis and infantile pneumonia. c. pneumoniae (twar) and c. psittaci also cause pneumonia and other respiratory infections. serum samples from 223 japanese infants and children with pneumonia were tested for igm antibodies to c. trachomatis, c. pneumoniae and c. psittaci. igm antibodies to c. trachomatis were measured by an enzyme immunoassay (eia) and by a microimmunofluorescence (mif) tes ...19921296808
purification of chlamydia psittaci antigen by affinity chromatography on polymyxin b agarose for use in the enzyme-linked immunosorbent assay (elisa).the purification of cell wall antigens of chlamydia psittaci by affinity chromatography on polymyxin b agarose is described. chlamydial cell wall antigens were prepared using different methods: heat treatment, ultrasonication and sodium deoxycholate treatment. the antigens were subsequently purified by gel chromatography. the highest amount of cell wall antigens was obtained by heat treatment of the chlamydiae at 90 degrees c and ph 8.5. the purified antigens showed molecular weights of 450 kda ...19921303687
evaluation of the polymerase chain reaction (pcr) for detection of chlamydia psittaci in abortion material from ewes.the polymerase chain reaction (pcr) was evaluated as a diagnostic tool for detection of chlamydia (c.) psittaci in abortion material from 40 ewes. for this purpose, pcr results of 87 samples were compared with direct microscopic identification after chemical staining, cell culture isolation and a commercially available enzyme-linked immunosorbent assay (elisa). the value for sensitivity as compared to cell culture was 97.7% whereas the specificity-value was calculated to be 84.1%.19921303688
serological survey for bovine bacterial and viral pathogens in captive arabian oryx (oryx leucoryx pallas, 1776).tests for antibodies to bovine bacterial and viral pathogens were conducted on 239 sera from 128 arabian oryx (oryx leucoryx) from seven locations (taif, riyadh and mahazat as said, saudi arabia; san diego, united states of america [usa]; shaumari, jordan; qatar; and bahrain). no antibodies to pasteurella multocida type e or epizootic haemorrhagic disease 1 virus were found. antibodies to brucella abortus, p. multocida type b, p. multocida type d, lumpy skin disease virus and akabane virus were ...19921305861
similarity of chlamydia pneumoniae strains in the variable domain iv region of the major outer membrane protein gene.dna was amplified by polymerase chain reaction from the gene encoding the major outer membrane protein (momp) of chlamydia pneumoniae in order to examine the relatedness of strains isolated from diverse geographical regions. primers for this reaction were chosen to span a 207-bp region comparable to that of the fourth variable segment of the momp gene of chlamydia trachomatis. among c. trachomatis, sequence heterogeneity is characteristic within variable sequence domain iv (vdiv) and correlates ...19921339411
[biological products and laboratory reagents dehydrated by a rapid direct vacuum desiccation method. vi. studies of the preservation of microbial strains (c. burnetii, chlamydia psittaci, mycoplasma pneumoniae) and a laboratory reagent (para-nitrophenyl phosphate) by vacuum desiccation]. 19921340260
biological properties and genetic analysis of the ompa locus in chlamydiae isolated from swine.eight strains of chlamydia psittaci isolated from swine with pneumonia, pleuritis, pericarditis, and enteritis were characterized through analysis of the major outer membrane protein gene ompa by a two-step polymerase chain reaction, by their interactions with cells in culture, and by the morphologic features and ultrastructure of intracellular inclusions. amplified chlamydial ompa dna fragments were differentiated by restriction endonuclease digestion. chlamydial isolates were separated into 2 ...19921358014
pcr detection and differentiation of chlamydia pneumoniae, chlamydia psittaci and chlamydia trachomatis.a pcr-based system was developed for the detection and differentiation of chlamydia trachomatis, chlamydia psittaci and chlamydia pneumoniae. a conserved 145 bp fragment of the chlamydial omp1 gene was amplified from all three species. the three species were then differentiated from each other by digestion of this pcr product with restriction enzymes eco ri and either hind iii or pst i. the system was shown to work for two strains of c. pneumoniae, 11 strains of c. psittaci and 10 serovars of c. ...19921361961
a synthetic glycoconjugate representing the genus-specific epitope of chlamydial lipopolysaccharide exhibits the same specificity as its natural counterpart.the tetrasaccharide 3-deoxy-alpha-d-manno-2-octulosonic acid (alpha-kdo) (2----8)-alpha-kdo(2----4)-alpha-kdo(2----6)-beta glcnac, a partial structure of chlamydial lipopolysaccharide (lps) representing a genus-specific epitope, was synthesized and covalently linked to bovine serum albumin, resulting in an artificial glycoconjugate antigen. mice were immunized with the glycoconjugate to prepare chlamydia-specific monoclonal antibodies. they were selected with chlamydia-specific lps antigens and ...19921372290
identification of chlamydia pneumoniae by dna amplification of the 16s rrna gene.chlamydia pneumoniae is an important cause of respiratory disease in humans, but diagnosis of c. pneumoniae is hindered by difficulties in the in vitro growth of the organism. in order to improve detection and identification, we recently developed a polymerase chain reaction (pcr) assay which uses oligonucleotide primers specific for c. pneumoniae. the nucleic acid sequence was determined for the 16s rrna of c. pneumoniae, and regions in which c. pneumoniae differed from both chlamydia psittaci ...19921374077
immunoelectron microscopy of chlamydia psittaci with monoclonal antibodies.an immunoelectron microscopic study was performed to determine the distribution of antigenic components on particles of chlamydia psittaci and infected cells using a number of monoclonal antibodies (mabs). of three anti-lipopolysaccharide (lps) antibodies (4d5, a2 and 4g5), two antibodies (4d5 and a2) reacted with the surface of reticulate bodies (rbs) but not with that of elementary bodies (ebs). the other antibody (4g5) reacted with both ebs and rbs. examination of infected cells in thin secti ...19921374830
detection and antigenicity of chlamydial proteins that bind eukaryotic cell membrane proteins.chlamydia psittaci proteins capable of binding eukaryotic cell membranes were identified and antigenically characterized. cell membrane proteins (cmp) of noninfected cells were labeled with biotin (b-cmp), then were extracted with 1% triton x-100. nitrocellulose membrane strips containing sodium dodecyl sulfate-polyacrylamide gel electrophoresis-resolved proteins of chlamydial elementary bodies (eb) were reacted with the b-cmp extract, followed by addition of streptavidin-conjugated horse radish ...19921378251
keratitis in free-ranging koalas (phascolarctos cinereus) on magnetic island, townsville.seventy free-ranging koalas (phascolarctos cinereus) from magnetic island (queensland, australia) underwent an ocular examination, blood collection and serological examination for chlamydia psittaci antibodies, and an examination of their teeth and genitalia. in 12 koalas long-standing unilateral keratitis was noted and in another 10 animals long-standing bilateral keratitis was observed. all animals were seronegative for chlamydia psittaci and apart from some nasal discharge and a variety of as ...19921380994
chlamydial endocarditis: a report on ten cases.over the period 1983-1990, 10 cases of infective native-valve endocarditis as a result of chlamydia were seen. all patients were men, with a mean age of 42 years, and none had a history of exposure to chlamydia psittaci. symptoms, such as weight loss and anorexia, with fever in eight cases, had persisted for at least 2 months before admission. haemodynamic failure was present in seven patients, and neurological signs in four. the aortic valve was involved in seven cases, the mitral valve in one ...19921396819
prevalence of chlamydia psittaci infection among persons who work with birds. 19921399508
[familial outbreak of pneumonia by psittacosis].a familial outbreak (4 cases) of pneumonia by psittacosis in vall de uxó (castellón) during the month of april in 1990 is described. in addition to the epidemiologic, clinical and serologic (complement fixation) studies carried out a survey and serology of 11 healthy controls (neighbors, and employees from a bird shop) were performed. the serum of a parrot recently acquired by the patients (positive at 1/8) was analyzed. the habit of petting the animal was significantly associated with the disea ...19921405807
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