| in vitro effect of leukocytic and thymic histones and their fractions on the activity of the causative agent of meningopneumonia. | it was found in studying the antimeningococcus activity of the leukocytic and thymus histones and their fractions that both histones were capable of neutralizing in vitro the activity of the causative agent of meningopneumonia (mp). the neutralization effect was chiefly associated with the f3 fraction rich in arginine and depended on the duration of the histone fraction contact with the mp causative agent, the weight concentration of the histone and the ph of the incubation medium. | 1975 | 1947 |
| antigenic analysis of chlamydiae by two-dimensional immunoelectrophoresis. ii. a trachoma-lgv-specific antigen. | two-dimensional immunoelectrophoresis was utilized to study precipitins in hyperimmune rabbit serum made against chlamydiae and from patients with chlamydial infections. an antigen of triton x-100-solubilized l2/434/bu organisms with an electrophoretic mobility of 0.65 relative to bovine serum albumin at ph 8.6 was excised from the agarose gel of electrophorograms as antigen-antibody complexes and used to immunize rabbits. a monospecific antiserum to antigen 0.65 was obtained that reacted with t ... | 1975 | 51883 |
| [use of the indirect hemagglutination test in the study of ornithosis. i. hemosensitizing activity of ornithosis phosphlipid antigen and a method of performing tests with it]. | a possibility was shown of using the phospholipid group-specific antigen of the ornitosis causative agent in the capacity of a hemosensitin for conducting the indirect hemagglutination test (ihat). there was revealed a direct proportional relationship between the sensitizing activity and the complement fixing properties of the antigens obtained. a method of sorption of this antigen on the tannin-treated erythrocytes and the optimal parameters of conducting the test with it were elaborated. a suf ... | 1976 | 66827 |
| [participation of species specific and group specific ornithosis antigens in immune reactions]. | the immunogenic properties of the species-specific antigen localized in the elementary particle membrane and group-specific or inner antigen of the causative agent of ornithosis were studied. the species specific antigen was shown to induce the antibody neutralizing the infectious properties of the agent as well as those agglutinating elementary bodies, inhibiting hemagglutination, and complement-fixing antibody detectable in the direct and indirect complement-fixation tests. the results indicat ... | 1978 | 86239 |
| [detection of humoral antibodies to species-specific and group ornithosis antigens in the dynamics of the infection]. | the time course of antibody production to group- and species-specific antigens of ornithosis agent was studied by complement fixation (cft) and hemagglutination-inhibition (hi) tests. in rabbits after a single intravenous inoculation of the ornithosis agent, antibody to the homologous species-specific antigen appeared in the peripheral blood 3--5 days after inoculation and reached maximum levels during the 1-st week, and to the group-specific antigen could be detected 3--5 days after inoculation ... | 1979 | 90431 |
| [comparative study of various chlamydia strains--preliminary study (author's transl)]. | | 1979 | 94564 |
| comparison of direct and modified direct complement-fixation and agar-gel precipitin methods in detecting chlamydial antibody in wild birds. | detection of chlamydial antibody in serums of wild birds was compared for the following methods: direct complement-fixation (dcf), modified dcf (mdcf), and agar-gel precipitin (agp). the birds species used were great-tailed grackles (cassidix mexicanus), common grackles (quiscalus quiscula), brown-headed cowbirds (molothrus ater), bronzed cowbirds (tangavius aeneus), and mourning doves (zenaida macroura). the birds were either inoculated with an isolate of chlamydia psittaci obtained originally ... | 1978 | 100098 |
| morphological and cytochemical study of chlamydia with edta regressive technique and gautier staining in ultrathin frozen sections of infected cell cultures: a comparison with embedded material. | the cryo-ultramicrotomy technique was applied to study the ultrastructure of chlamydia using two strains: one of c. psittaci and one of c. trachomatis. it clearly appeared that in both strains reticulate bodies show a high degree of plasticity, contrasting with the rigid spherical appearance of elementary bodies. ultrastructural cytochemical study shows dna fibrils dispersed throughout the cytoplasm in reticulate bodies whereas dna is condensed in a nucleoid in elementary and intermediate bodies ... | 1978 | 106752 |
| from the center for disease control: psittacosis in humans in the united states, 1975--1977. | | 1979 | 222851 |
| growth and effect of chlamydiae in human and bovine oviduct organ cultures. | | 1979 | 223717 |
| chlamydiales: properties, cycle of development and effect on eukaryotic host cells. | | 1977 | 334482 |
| infections due to chlamydia. | | 1977 | 336858 |
| [early diagnosis and the antibacterial treatment of acute pneumonia in adults]. | | 1977 | 337530 |
| chlamydial infections (third of three parts). | | 1978 | 342952 |
| parasite-specified phagocytosis of chlamydia psittaci and chlamydia trachomatis by l and hela cells. | phagocytosis of the 6bc strain of chlamydia psittaci and the lymphogranuloma venereum 440l strain of chlamydia trachomatis by l cells and hela 229 cells occurred at rates and to extents that were 10 to 100 times greater than those observed for the phagocytosis of escherichia coli and polystyrene latex spheres. both species of chlamydia were efficiently taken up by host cells of a type they had not previously encountered. phagocytosis of chlamydiae was brought about by the interaction of parasite ... | 1978 | 344217 |
| [use of the immunofluorescent microagglutination reaction for the purpose of studying ornithosis infection. 1. production and approbation of a corpuscular luminescent diagnosticum]. | a method of obtaining the fluorescent ornithosis corpuscular diagnostic agent providing for the extraction of the corpuscular antigen with its subsequent conjugation with fluorochrome-fluoresceine isothiocyanate. the use of this preparation permits to stage the agglutination reaction on the basis of immunofluorescent analysis, this facilitating the recording of the reaction results and considerably decreasing consumption of the diagnostic agent. as shown, the suggested immunofluorescent microagg ... | 1978 | 371295 |
| chlamydial infections. | | 1979 | 379774 |
| prevalence of certain antibodies to selected disease-causing agents in wild turkeys in texas. | in texas in 1976 and 1977, rio grande turkeys (meleagris gallopavo intermedia) from seven counties and eastern turkeys (m. g. silvestris) from one county were tested for antibodies to selected poultry pathogens. standardized serological tests disclosed reactors to salmonella pullorum (2.4%), s. typhimurium (2.3%), and mycoplasma gallisepticum (4.8%). there were no reactors to newcastle disease virus or chlamydia psittaci. prevalence of m. gallisepticum antibody in wild turkeys was significantly ... | 1979 | 384989 |
| chlamydial infections. microbiological, clinical and diagnostic aspects. | the agent of trachoma, originally regarded as a virus, was described by halberstadter and von prowacek in 1907. since then, studies on chlamydiae have shown that they are procaryocytic organisms that infect eucaryocytic cells as obligate intracellular parasites. infections caused by these pathogenic agents occur in a wide range of avian and mammalian species. chlamydiae are also responsible for several diseases in man, and there is a rapidly growing awareness of their public health significance. ... | 1979 | 388102 |
| [causative agent of benign lymphoreticulosis]. | a causative agent ("d" strain) was isolated from the contents of an affected lymph node of a patient with beningn lymphoreticulosis. according to the biological and antigenic properties this agent is identified with chlamydia but is a distinct species. the chlamydial etiology of benign lymphoreticulosis is determined also by the complement-fixation test with sera from convalescents. | 1977 | 411261 |
| human cell-mediated immune responses to chlamydial antigens. | a reproducible method was developed to determine the ability of chlamydial antigens to stimulate lymphocytes from volunteers. in tests repeated 4 to 14 times, the cells from a given volunteer gave a relatively narrow range of responses, but there were great differences in the mean response of different volunteers. in the entire group of 52 volunteers, lymphocyte stimulation was significantly associated with the presence of antibody, but in a given individual results of one test did not aid in pr ... | 1979 | 422247 |
| [use of a cryoultramicrotomy method for the purpose of studying chlamydial ultrastructure]. | the method of cryoultramicrotomy was adapted for the study of the ultrastructure of hela and mccoy cells in monolayer cultures infected with chlamydia, obligatory intracellular procaryotic parasites, the causative agents of ornithosis (strain loth) and paratrachoma (strain lb 1). the cryosections were obtained by the fixation of the monolayer with 2.5% glutaraldehyde, by the gradual infiltration of precipitated cells with sucrose (0.6--1.2--1.8--2.3 m) prior to freezing in liquid nitrogen, and b ... | 1979 | 433503 |
| arrays of hemispheric surface projections on chlamydia psittaci and chlamydia trachomatis observed by scanning electron microscopy. | scanning microscopy of two strains of chlamydia psittaci and four strains of chlamydia trachomatis representative of the wide diversity in origin and behavior of members of the genus revealed patches of regular arrays of hemispheric projections on the surfaces of elementary bodies of all six strains. these distinctive and perhaps unique surface structure are probably present in all populations of chlamydiae. | 1979 | 438131 |
| biotyping of chlamydia psittaci based on inclusion morphology and response to diethylaminoethyl-dextran and cycloheximide. | strains of chlamydia psittaci from cattle, sheep, pigs, mice, guinea pigs, rabbits, cats, and parrots were subdivided based on their biological characteristics. chlamydiae grown in the yolk sac of chicken embryos were used to infect l cell monolayers. the host cells were infected without further treatment or treated with diethylaminoethyl-dextran, cycloheximide, or both. the following criteria were used for biotyping the strains: the morphology of the inclusions and time after infection at which ... | 1979 | 457272 |
| chlamydia: the organism and neonatal infection. | | 1979 | 478514 |
| wheat germ agglutinin blockage of chlamydial attachment sites: antagonism by n-acetyl-d-glucosamine. | addition of 2 to 10 micrograms of wheat germ agglutinin (wga), a lectin from triticum vulgaris specific for n-acetyl-d-glucosamine, per ml to suspensions of mouse fibroblasts (l cells) blocked the attachment of 14c-labeled chlamydia psittaci 6bc to the l-cell surface. wga and strain 6bc competed for similar sites on l cells, but once bound, one was not replaced by the other. n-acetyl-d-glucosamine, but not other monosaccharides of related structure, antagonized the blocking action of wga. lectin ... | 1979 | 500195 |
| [use of the indirect hemagglutination reaction in studying ornithosis infection. iii. the diagnostic value of the ihr with an ornithosis erythocytic diagnosticum]. | in both experimental and clinical conditions the passive hemagglutination test (phat with the use of an ornithosis erythrocytic diagnostic preparation was found to be sufficiently sensitive and specific as compared with the complement fixation test (cft), a routine testing method. the study of the dynamics of immune response in infected animals and ornithosis patients allowed to regard the phat as a comparatively early method of serological analysis. hemagglutinins were also found to circulate i ... | 1979 | 433499 |
| serological cross-reactions between acinetobacter calcoaceticus and chlamydiae. | a cross-reaction between acinetobacter calcoaceticus and chlamydiae is described. a water-soluble, heat stable, non-dialyzable antigen was extracted from acinetobacter species by boiling. this antigen fixed complement in the presence of homologous hyperimmune sera from rabbits or guinea pigs and in the presence of heterologous human or hyperimmunized animal sera containing chlamydial antibodies. hyperimmune antisera to the extracted antigen, or to suspensions of live acinetobacters, also reacted ... | 1979 | 521480 |
| demonstration of chlamydial endotoxin-like activity. | | 1979 | 521792 |
| isolation of a chlamydial agent from rocky mountain bighorn sheep. | a total of 53 clinical specimens from both healthy and diseased rocky mountain bighorn sheep (ovis canadensis) were examined for chlamydia. an agent consistently lethal for chicken embryos was recovered from a nasal swab taken from a normal ewe. this agent, designated bhs-15, possesses antigens which fix complement in the presence of anti-chlamydial serum, is susceptible to chlortetracycline, and is resistant to sodium sulfadiazine and streptomycin. attempts to culture the isolate in quality con ... | 1979 | 522218 |
| isolation of chlamydia psittaci ovis in sheep thyroid cell culture. | | 1979 | 433110 |
| experimental chlamydial salpingitis in immunosuppressed guinea pigs infected in the genital tract with the agent of guinea pig inclusion conjunctivitis. | at necropsy indication of spread of infection to fallopian tubes was found in 25 of 41 (60%) female guinea pigs infected in the genital tract with the chlamydial agent of guinea pig inclusion conjunctivitis and immunosuppressed with cyclophosphamide. eighteen were examined histologically, and the diagnosis of acute salpingitis was confirmed in 10, based on inflammatory reaction, detection of guinea pig inclusion conjunctivitis in tissue, and formation of cysts (pyosalpinx and hydrosalpinx). infe ... | 1979 | 397933 |
| [the replication cycle of chlamydia trachomatis and chlamydia psittaci: ultrastructural analysis]. | an ultrastructural comparative analysis is reported on the replication cycle of six strains of chlamydia trachomatis, recently isolated, and chlamydia psittaci strain 6bc, grown in cell cultures. important morphological distinctions of the bacterial walls are documented. in chlamydia trachomatis the cell wall seems more rigid. this causes, during the morphogenesis of the elementary body, its separation from the cell membrane with formation of an electron transparent space, which is not demonstra ... | 1979 | 553521 |
| target tissues associated with genital infection of female guinea pigs by the chlamydial agent of guinea pig inclusion conjunctivitis. | female guinea pigs were experimentally infected in the genital tract with the strain of chlamydia psittaci that causes guinea pig inclusion conjunctivitis. chlamydiae were found frequently in superficial squamous epithelial cells of the exocervix, along with heavy involvement at the squamocolumnar junction. in this zone chlamydiae were observed in columnar cells, but inflammation and chlamydial inclusions were not observed in true endocervical epithelium. these observations were supported by res ... | 1979 | 374637 |
| kinetics of the complement fixing and immunofluorescent antibody response in experimental chlamydiosis in ewes. | normal, 70 days pregnant ewes were inoculated i.v. with a chicken embryo grown chlamydia psittaci strain. the ewes presented a biphasic febrile curve and aborted 30 to 68 days after inoculation. chlamydiae were isolated from aborted fetuses and from vaginal swabs. ewes developed cf and if antibodies titrating 1:320 to 1:1024 two to three weeks after inoculation. a second antibody rise occurred one to two weeks before or after abortion and reached titers of 1:640 to 1:1024. immunoglobulin fractio ... | 1978 | 373577 |
| [chlamydiosis in sheep and cattle in south africa (author's transl)]. | | 1977 | 563917 |
| sensitivity of immunoperoxidase and immunofluorescence staining for detecting chlamydia in conjunctival scrapings and in cell culture. | the sensitivities of giemsa, immunofluorescence, and immunoperoxidase staining for the detection of chlamydia psittaci inclusions in conjunctival scrapings and in irradiated mccoy cell monolayers were compared. conjunctival specimens were obtained from a cat colony in which a trachoma-like disease, feline chlamydial keratoconjunctivitis, was endemic. the two immunochemical techniques were found to be of equal sensitivity and 50% to 100% more sensitive than giemsa stain. permanent preparations of ... | 1978 | 368085 |
| legionnaires' disease: antigenic peculiarities, strain differences, and antibiotic sensitivities of the agent. | paired sera from victims of legionnaires' disease showed, in many cases, significant rises in immunoglobulin g antibodies to both the causative agent (la) of legionnaires' disease and chlamydia psittaci, but concurrent rises in immunoglobulin m antibodies only against la. guinea pigs experimentally infected with la likewise responded with antibodies to both c. psittaci and la. guinea pigs infected with la also reflected significant differences in antigenic makeup and in pathogenicity among four ... | 1978 | 355583 |
| [plaque assay for chlamydia psittaci in tissue samples (author's transl)]. | direct isolation of chlamydia psittaci (var, ovis) from mouse spleen was performed in parallel by plaque assay on mccoy cells and by staining of cytoplasmic inclusions in hela 229 cells. there was a significant difference between the frequency of isolation: the plaque technique was highly sensitive and reproducible; it offers a potential tool for investigations requiring the accurate quantitation of chlamydiae in tissue sample. | 1977 | 334017 |
| pathogenesis of chlamydial polyarthritis in domestic animals: characteristics of causative agent. | | 1979 | 263453 |
| [isolation of the causative agent of ornithosis from gamasid mites]. | a microorganism was isolated from gamasoid mites (ornithonyssus sylviarum) collected in nests of blue rock pigeons by means of blind passages in white mice and chick embryos. the studies of its morphological, cultural, and antigenic properties and pathogenicity for laboratory animals permitted to identify it as the causative agent of ornithosis. it is suggested that the possibility of infection of ectoparasites is determined to a considerable extent by the degree of activity of the infectious pr ... | 1979 | 160679 |
| [chlamydia--a new pathogen]. | | 1977 | 590158 |
| [effect of leukocyte cation proteins in vitro on the activity of the pathogen of meningopneumonitis]. | a study was made of the influence of cation proteins from the lysosomes and nuclei of rabbit polymorphonuclear leukocytes on the survival of the causative agent of meningopneumonia in vitro. as revealed, the greatest antibiotic activity was possessed by the nuclear histones and the low molecular basic proteins of lysosomes. biological significance of the detected effects is discussed. | 1977 | 596028 |
| [results of identifying ornithosis strains isolated in tajikistan (author's transl)]. | studies were carried out aimed at isolation of the causative agent of ornithosis from the organs of pigeons and budgerigars. the present study was based on the results of a serological survey which had revealed a high rate of infection with ornithosis in pigeons as well as a case of ornithosis in an owner of budgerigars. the isolates were identified, their pathogenicity for some animal species was determined; morphological studies were carried out in cell culture; antigens and immune sera were p ... | 1975 | 128906 |
| division of single host cells after infection with chlamydiae. | mouse fibroblasts (l cells) were infected in suspension with chlamydia psittaci (6bc) and then plated out on a solid substrate at a density of 80 cells per cm2 so that the effect of chlamydial infection on the division of single host cells and their progeny could be determined. uninfected l cells multiplied with a mean generation time of 15 h. the generation time of single l cells infected with 1.5 50% infectious units (id50) of c. psittaci was over twice as long. half of the infected l cells ha ... | 1978 | 624590 |
| [acute meningo-encephalitis in adolescents and adults--i. germs, rate of morbidity and lethality (author's transl)]. | diverse forms of acute meningo-encephalitis found in adolescents and adults are discussed considering 1244 cases treated at the neurologic clinic of the university at cologne in the time from 1950 till 1975. the rate of morbidity and lethality was found to vary according to the type of causative germ. the inflammation often took another course than in childhood. the age at the outbreak of the disease, infection immunity, precursing and concomitant illnesses as well as the onset of medication inf ... | 1979 | 118326 |
| [isolation and identification of clamydia psittaci as the pathogen in enzootic abortion of sheep in eastern slovakia]. | abortions in ewes occurred on a large scale in three localities in eastern slovakia. antibodies to the group-type ornithosis antigen were detected in titres of 1 : 128 to 1 : 2048 in the aborting ewes in the mentioned localities. suspensions were prepared from the four samples of material, obtained either from the placentae and afterbirths of the aborting ewes or from the tissues of the aborted foetuses. seven-day old yolk sacs of chicken embryos were infected with these suspensions. four strain ... | 1978 | 99861 |
| chlamydia psittaci: growth characteristics and enumeration of serotypes 1 and 2 in cultured cells. | the growth characteristics of chlamydia psittaci serotypes 1 and 2 (ovine and bovine origin) were studed in mouse l cells. formation of inclusions and yield of infectious progeny for serotype 1 were maximal when host cells were treated with cycloheximide and the ph in the cell culture medium was 7.2-7.4. the number of cells that contained inclusions and the infectivity yield for serotype 2 were maximal when the ph was 6.6-7.0. treatments with diethylaminoethyl dextran and cycloheximide increased ... | 1979 | 94339 |
| direct immunofluorescence tests with counterstains for detection of chlamydia psittaci in infected avian tissues. | different methods of preparation and serological evaluation of rabbit globulins for use in fluorescent antibody conjugate and different methods of counterstaining with fluorescent antibody tests were evaluated for detection of chlamydia psittaci in infected turkey tissues. the agar gel precipitin reaction was that chosen for testing and selecting antiserums to be used for fluorescein isothiocyanate conjugation. the fluorescent antibody staining was most pronounced with conjugate made from globul ... | 1979 | 91417 |
| loss of inorganic ions from host cells infected with chlamydia psittaci. | mouse fibroblasts (l cells) infected with the 6bc strain of chlamydia psittaci released potassium ion (k(+)) into the extracellular milieu in a way that depended on size of inoculum and time after infection. when the multiplicity of infection was 500 to 1,000 50% infectious units (id(50)) per l cell, loss of intracellular k(+) was first apparent 4 to 10 h after infection and was nearly complete at 6 to 20 h. magnesium ion and inorganic phosphate (p(i)) were also released. similar multiplicities ... | 1978 | 640730 |
| enzyme-linked immunosorbent assay for chlamydial antibodies. | an enzyme-linked immunosorbent assay (elisa) detected chlamydial antibodies in human sera. the assay antigen produced in cell cultures infected with chlamydia psittaci was formalin-fixed to microplates. single convalescent-phase sera positive for chlamydial antibodies by a complement-fixation test were positive at even higher dilutions by elisa. paired sera with diagnostic rises in complement-fixing antibody showed seroconversion by elisa also. control sera from persons with no history of chlamy ... | 1977 | 72759 |
| experimentally induced feline chlamydial infection (feline pneumonitis). | cats exposed to aerosols of feline chlamydia psittaci developed a disease characterized principally by conjunctivitis. signs of conjunctivitis appeared between postexposure days (ped) 5 and 10, were often unilateral initially, and persisted for 22 to 45 days. fever followed the onset of conjunctivitis (ped 11 to 15) and persisted for 3 to 8 days. signs of mild rhinitis (occasional sneezing and mild serous nasal discharge) occurred in some cats between ped 8 and 37. neither signs of lower respira ... | 1978 | 646190 |
| ultrastructural studies of the nucleoids of the pleomorphic forms of chlamydia psittaci 6bc: a comparison with bacteria. | the nucleoids of the various pleomorphic forms of chlamydia psittaci have been examined by direct observation of infected cells and by observations on isolated particles. the fixation and staining methods used were the same as those routinely used for the examination of bacteria to facilitate the comparison of chlamydial fine structure with that of bacteria. the nucleoids of reticulate bodies were composed of fine fibrils which extended throughout these particles. the nucleoids of intermediate b ... | 1976 | 56212 |
| occupational diseases of the lungs. | | 1975 | 50754 |
| mechanisms of infertility in genital tract infections due to chlamydia psittaci transmitted through contaminated semen. | ten heifers inseminated with semen artificially contaminated with chlamydia psittaci were not pregnant 40 days later, whereas five of 10 control heifers inseminated with the same semen mixed with control diluent became pregnant. normal embryos were recovered two and one-half or three days after insemination of another group of animals with semen containing c. psittaci, a finding indicating that fertilization failure was not responsible for the infertility. uterine biopsy samples taken from two h ... | 1978 | 681792 |
| [endocarditis and aortic valve insufficiency caused by ornithosis]. | | 1978 | 688959 |
| protein-carbohydrate-lipid complex isolated from the cell envelopes of chlamydia psittaci in alkaline buffer and ethylenediaminetetraacetate. | exposure of isolated cell envelopes from purified infectious elementary (eb) of chlamydia psittaci to sodium carbonate-bicarbonate buffer at ph 10 plus ethylenediaminetetraacetate (edta) results in partial solubilization of the total protein. the released materials represent 20% of the dry weight, 16% of the total protein, 40% of the total carbohydrate, and 9% of the total lipid of the cell envelopes. sucrose density gradient centrifugation, and sephadex g-200, sepharose 4b, or diethylaminoethyl ... | 1976 | 1376 |
| effect of alkali on the structure of cell envelopes of chlamydia psittaci elementary bodies. | suspensions of isolated cell envelopes of infectious elementary bodies (eb) of chlamydia psittaci at alkaline ph showed a rapid, extensive decrease in absorbance, accompanied by the release of a cell envelope component in a sedimentable form. this phenomenon was observed both at 0 c and with envelopes which had been previously heated to 100 c. monovalent and divalent cations effectively inhibited the turbidity loss, whereas ethylenediaminetetraacetate (edta) caused an accelerated decrease in tur ... | 1976 | 1375 |
| [ornithosis agents as an etiological factor in atypical pneumonia]. | | 1978 | 714775 |
| infective endocarditis with glomerulonephritis associated with cat chlamydia (c. psittaci) infection. | a patient with glomerulonephritis and endocarditis is described who had evidence of feline chlamydia psittaci infection. treatment with antichlamydial drugs resulted in resolution of the glomerulonephritis and the endocarditis. it is recommended that screening for chlamydia is included in the investigation of patients with suspected or obscure endocarditis. | 1979 | 508458 |
| use of hela cell guanine nucleotides by chlamydia psittaci. | exogenous guanine was found to be incorporated into the nucleic acids of chlamydia psittaci when the parasite was grown in hela cells containing hypoxanthine guanine phosphoribosyltransferase (ec 2.4.2.8) activity but not when the parasite was grown in transferase-deficient hela cells. no evidence for a chlamydia-specific transferase activity was found in either transferase-containing or transferase-deficient infected hela cells. it is concluded that c. psittaci is incapable of metabolizing guan ... | 1979 | 478649 |
| cytochalasin b does not inhibit ingestion of chlamydia psittaci by mouse fibroblasts (l cells) and mouse peritoneal macrophages. | cytochalasin b did not inhibit ingestion of chlamydia psittaci by either mouse fibroblasts (l cells) or mouse peritoneal macrophages in concentrations that produced distinctive morphological changes and inhibited phagocytosis of polystyrene latex beads and escherichia coli k-12. | 1979 | 478646 |
| chlamydia shedding by four species of wild birds. | four wild bird species--great-tailed grackle (cassidix mexicanus), common grackle (quiscalus quiscula), brown-headed cowbird (molothrus ater), and mourning dove (zenaidura macroura)--were either inoculated intratracheally with chlamydia psittaci or exposed indirectly as uninoculated cagemates. shedding of chlamydiae was monitored by inoculating mice with suspensions of material eluted from cloacal swabs collected from all birds, usually at 3-day intervals. sporadic shedding of chlamydiae was dem ... | 1978 | 749892 |
| chlamydiae, cervicitis, and abnormal papanicolaou smears. | cervical abnormalities accompanied by papanicolaou class ii or class iii cytologic results are commonly encountered in gynecologic office practice. chlamydiae are a common cause of genital tract infection, with or without manifest symptoms or signs. an immunofluorescence method was used to determine the presence of antichlamydial antibodies in cervical secretions. eleven of 15 patients in whom such antibodies were found (73.3%) had papanicolaou class ii or class iii smears, in contrast to only 3 ... | 1979 | 760016 |
| changes in the ultrastructure of chlamydia psittaci produced by treatment of the host cell with deae-dextran and cycloheximide. | | 1979 | 469985 |
| interaction of chlamydia psittaci reticulate bodies with mouse peritoneal macrophages. | noninfectious reticulate bodies of chlamydia psittaci are readily phagocytized by thioglycolate-elicited mouse peritoneal macrophages in monolayer culture. the internalized reticulate bodies are rapidly destroyed as indicated by a 60 to 70% decrease in trichloroacetic acid-precipitable radioisotopic counts in the macrophage pellet by 10 h and a concomitant increase of the trichloroacetic acid-soluble radiolabeled chlamydial nucleic acid in the cytoplasm. this intracellular destruction of reticul ... | 1979 | 468374 |
| experimental pneumonia in red deer (cervus elaphus l) produced by an ovine chlamydia. | an isolate of chlamydia psittaci from ovine pneumonia produced extensive pneumonia in red deer after endobronchial inoculation. associated clinical signs lasting for several days included pyrexia, inappetance, increased pulse and respiration rate, and physical distress after handling, but no coughing or upper respiratory symptoms. histologically, an acute exudative reaction was present after two days, and an early proliferative response after seven days. both the clinical and pathological respon ... | 1979 | 532073 |
| utilization of exogenous thymidine by chlamydia psittaci growing in the thymidine kinase-containing and thymidine kinase-deficient l cells. | the incorporation of [3h]thymidine into the deoxyribonucleic acid (dna) of chlamydia psittaci (strain 6bc) growing in thymidine kinase (adenosine 5'-triphosphate-thymidine 5'-phosphotransferase, ec 1.7.1.21)-containing l cells, l(tk+), and thymidine kinase-deficient l cells, lm(tk-), was examined by autoradiography. label was detected over c. psittaci inclusions in l(tk+) but not lm(tk-) cells. no evidence for a chlamydia-specific thymidine kinase activity in either l(tk+) or lm(tk-) cells was o ... | 1976 | 942715 |
| humoral immunity in the resolution of genital infection in female guinea pigs infected with the agent of guinea pig inclusion conjunctivitis. | female guinea pigs infected in the genital tract with the chlamydial agent of guinea pig inclusion conjunctivitis were selectively immunosuppressed by varying regimens of cyclophosphamide (cy) treatment. temporary suppression of both humoral and cell-mediated immunity by daily treatment of cy (25 mg/kg) for 13 days resulted in a prolonged infection, whereas daily treatment for the duration of the experiment totally prevented the development of humoral and cell-mediated responses and produced an ... | 1979 | 546788 |
| characterization of miniature pig kidney cells and their resistance to chlamydial infection. | a miniature pig kidney cell line has been established from porcine fetuses taken aseptically by hysterectomy and maintained for more than 50 passages in eagle's minimal essential medium containing 10% heat-inactivated newborn calf serum. cell transfers were performed each week. primary and serially passaged cells were found to be highly refractory to infection by chlamydia trachomatis strains tw-3, bour, and lgv 440l and chlamydia psittaci strains meningopneumonitis and 6bc and insusceptible to ... | 1976 | 944002 |
| the use of cell cultures from the chorioallantoic membrane of chick embryos for studies of chlamydiae. | cells of the chorioallantoic membrane of chick embryos were processed into monolayer cell cultures by a recently described technique of cursiefen and brecht. the cultures provided a sensitive substrate for the quantification of infectivity of c. psittaci strain 6bc and meningo-pneumonitis. | 1977 | 559536 |
| sucrose density differences of chlamydia psittaci 6bc in relation to its host. | previous studies on chlamydia psittaci 6bc propagated in different hosts have shown differences in cytotoxicity but no differences in the ultrastructure of the individual particles. it is shown here that the 6bc strain derived from yolk sac of infected chick embryo sedimented in sucrose gradients at lower densities than the 6bc strain derived from l-cells. host-related modifications of lipid concentrations of the 6bc strains have been previously documented by others. it is thought that the pheno ... | 1977 | 559537 |
| differences in physicochemical and antigenic properties of chlamydial strains. | antigenic analysis of chlamydia psittaci, c. trachomatis, and lymphogranuloma venereum (lgv) revealed basic physicochemical differences among the three chlamydial strains. these were manifested in structural, isoelectric points, absorption spectra, and in the characteristics of the chromophobe-containing proteins. the effective period of sonication for c. psittaci and c. trachomatis is around 60 min during which the linkages most susceptible to external sonication forces were broken, releasing a ... | 1976 | 963615 |
| growth of chlamydia psittaci in macrophages. | survival and growth of l-cell-cultivated chlamydia psittaci occurred in mouse macrophages in vitro. two major factors governing the intracellular fate of chlamydiae in macrophages are: (i) the multiplicity of infection (moi), i.e., the elementary body (eb)-to-macrophage ratio, and (ii) the state of the eb. at a low moi (1:1) survival and growth of live, untreated chlamydiae were optimal. the chlamydiae were internalized in macrophages within 30 to 40 min. eb proceeded to differentiate into retic ... | 1978 | 565338 |
| interaction of chlamydia psittaci with mouse peritoneal macrophages. | l-cell-grown chlamydia psittaci elementary bodies (eb) were rapidly phagocytized by mouse peritoneal macrophages in vitro. however, the intracellular fate of chlamydiae in macrophages appeared to be dependent on the multiplicity of infection (moi), i.e., the eb-to-macrophage ratio, and the treatment of the eb. at an moi of 1:1 or less, survival is maximal, and growth and multiplication of live, untreated chlamydiae did occur. in contrast, at a high moi (100:1), survival of chlamydiae is reduced, ... | 1978 | 565339 |
| infectivity titration of guinea-pig inclusion conjunctivitis agent in irradiated mccoy cells. | estimation of the infectivity of the agent of guinea-pig inclusion conjunctivitis for irradiated mccoy cells, assayed as inclusion-forming units, was influenced by the age of cells after irradiation, the maturation time of the inclusions, the centrifugal force and the centrifugation temperature. agent passaged through irradiated mccoy cells or guinea-pig conjunctivae showed a greater capacity to infect irradiated mccoy cells without centrifuging than agent grown in a chick embryo. the nature of ... | 1976 | 986419 |
| [use of the indirect hemagglutination test for the study of ornithosis. report 2. preparation and approbation of the dry ornithosis erythrocyte diagnostic agent]. | an original preparation--dry ornithosis erythrocytic diagnostic agent for the indirect hemagglutination test was prepared on the basis of formalinized tannin-treated sheep red blood cells and group-specific phospholipid antigen of the causative agent of ornithosis. this diagnostic agent retained its specific activity for 18 months (observation period). the use of this preparation considerably facilitated the method of performance of this test, this offering a possibility of its wide application ... | 1978 | 566502 |
| prevalence of ornithosis and other chlamydia-like infections in the ukrainian ssr. | the prevalence of ornithosis and other chlamydia infections among various groups of the population, some species of birds and mammals was studied in several regions of the ukrainian ssr. among the town population having no occupational contact with domestic fowl and farm animals, complement-fixing antibodies to group ornithosis antigen were found in 6.5% of the examined, among the inhabitants of rural localities in 8.4% and among the workers of poultry farms in 19.9%. during the examination of t ... | 1979 | 575382 |
| [importance and distribution of serological findings of ornithosis-psittacosis in semi-domestic pigeons]. | ornithosis-psittacosis is a widely distributed disease in birds throughout the world. a serological study by the complement-fixation test and a few isolation of chlamyqia were performed on feral pigeon population of antwerpen city. the incidence of positive cases in antwerpen averages 58.51 %. for each serum, a serological index (s. i.) was calculated with regard to the intensity of immunological response in different dilutions: s. i. seems to suggest an endemic status of the disease in antwerpe ... | 1977 | 579614 |
| some physical properties of a sheep pneumonia strain of chlamydia psittaci. | | 1976 | 997192 |
| ultrastructural changes in host cellular organelles in the course of the chlamydial developmental cycle. | different stages in the chlamydial developmental cycle were correlated with cytopathic changes in host cellular organelles by ultrastructural analysis of infected cultured cells and cells of the intestinal mucosa of newborn calves. four distinct morphologic forms of chlamydial development can be distinguished: elementary bodies, dispersing forms, reticulate bodies and condensing forms which proceed to form elementary bodies. from the stage of elementary body uptake through formation and multipli ... | 1976 | 1015025 |
| antigenic analysis of chlamydiae by two-dimensional immunoelectrophoresis. i. antigenic heterogeneity between c. trachomatis and c. psittaci. | tw0-dimensional immunoelectrophoresis was used for the antigenic analysis of chlamydia. antigens of chlamydiae were found to be successfully solubilized with the nonionic detergent triton x-100. nineteen and 16 distinct components were observed for chlamydia trachomatis strain l2/434/bu and c. psittaci strain meningopneumonitis/cal-10, respectively. the triton-solubilized antigens of chlamydiae differ strikingly between species since only one strong reciprocal cross-reacting antigen was observed ... | 1975 | 1080772 |
| chlamydiae as agents of sexually transmitted diseases. | chlamydiae are being increasingly recognized as an important cause of human disease. the known geographical distribution of lymphogranuloma venereum and the role of chlamydiae as agents of sexually transmitted diseases are reviewed. the presence of chlamydiae in the urethra and the cervix, and their etiological relationship to genital infections, first recognized in connexion with ocular infections, have been proved in a number of studies in selected populations in a few countries. chlamydiae ap ... | 1976 | 1088107 |
| [detection of the antigens of the causative agent of enzootic ovine abortion by an immunoperoxidase method]. | | 1975 | 1108388 |
| [study of the specificity of various classes of immunoglobulins (igm and igg) v techenie ornitoznoĭ infektsii]. | studies of the specificity of immunoglobulins of different classes in the course of both experimental and natural orinthosis infection showed igg antibody to possess a wide spectrum of activity: group-, species- and strain-specific, but specificity to group antigenic components was predominant. igm antibodies were species-specific, however, population of gammam-immunoglobulins of animal sera in approximately 7 and 14 days after infection antibody suggested a method for the study of the antigenic ... | 1975 | 1108451 |
| [chlamydia psittaci infections among hospital personnel]. | | 1977 | 607278 |
| psittacosis: the reservoir persists. | during a one-year period, 101 parakeets and parrots were submitted for laboratory examination. the birds were sick, dead, or from premises where morbidity had been observed. tissue specimens from these birds were tested for the presence of chlamydiapsittaci by two methods. a tissue culture system using mccoy cells treated with 5-iodo-2-deoxyuridine was found to be more sensitive than intraperitoneal inoculation of mice for isolation of the chlamydiae. chlamydiae were recovered from 21 (34%) of 6 ... | 1978 | 624851 |
| chlamydia psittaci infection of horses with respiratory disease. | two strains of chlamydia psittaci were isolated from the nasal tract of horses with acute respiratory disease. these 2 isolates (ns 121 and ns 172) were characterized as chlamydia on the basis of their morphology, tinctorial property, growth in chicken embryos, inability to grow on bacterial media and their possession of chlamydial common complement fixing group antigen. they were identified as c. psittaci on the basis of resistance to sodium sulphadiazine. the present strains were not pathogeni ... | 1978 | 631105 |
| kinetics of phagocytosis of chlamydia psittaci by mouse fibroblasts (l cells): separation of the attachment and ingestion stages. | the kinetics of phagocytosis of chlamydia psittaci (6bc) by monolayers of mouse fibroblasts (l cells) was studied with an assay that distinguished between the attachment and ingestion phases of phagocytosis. at multiplicities of 10 and 100 50% infectious doses (id50) per l cell, virtually all of the inoculated c. psittaci had been attached and ingested after 60 min at 37 degrees c. at multiplicities of 500 to 5,000 id50 per l cell, the initial rates of attachment and ingestion of c. psittaci to ... | 1978 | 631892 |
| stimulation of cell-mediated immunity of chlamydiosis in turkeys by inoculation of chlamydial bacterin. | | 1978 | 637395 |
| [experimental infection of guinea pigs with chlamydia. 1. pathomorphological studies]. | the changes that occurred following nasal instillation of a bovine strain of chlamydia psittaci were characterized by hypertrophy and proliferation of epithelial cells and exudates containing polymorphonuclear leukocytes, with acute bronchopneumonia and less intense inflammatory reactions in nasal and tracheal mucosa, spleen and pulmonary lymph nodes. the intestines, liver, kidney and brain were scarcely affected. there was little prospect of complete recovery from the clinically mild, or even s ... | 1975 | 1227428 |
| surface projections and internal structure of chlamydia psittaci. | the outermost surface of the small infectious forms of chlamydia psittaci contain geometrically arranged spikes distributed over approximately 50% of the bacterial surface. the spikes are located opposite the concave side of an electron-dense crescent-shaped chlamydial core. | 1978 | 641015 |
| the isolation of a chlamydia psittaci-like agent from a free-living african buffalo (syncerus caffer). | | 1978 | 676013 |
| detection of igm antibodies to chlamydia trachomatis, chlamydia pneumoniae, and chlamydia psittaci from japanese infants and children with pneumonia. | chlamydia trachomatis (c. trachomatis) is now well established as a pathogen of neonatal inclusion conjunctivitis and infantile pneumonia. c. pneumoniae (twar) and c. psittaci also cause pneumonia and other respiratory infections. serum samples from 223 japanese infants and children with pneumonia were tested for igm antibodies to c. trachomatis, c. pneumoniae and c. psittaci. igm antibodies to c. trachomatis were measured by an enzyme immunoassay (eia) and by a microimmunofluorescence (mif) tes ... | 1992 | 1296808 |
| chlamydia pneumoniae respiratory infection in a child--a case report. | a case of respiratory infection in a child due to chlamydia pneumoniae is reported. the diagnosis was made by the detection of chlamydial antigen in the tracheal secretion and a significant increase in c. pneumoniae antibody titre. the infection responded well to erythromycin therapy. | 1992 | 1303485 |
| transmission of chlamydiae from grackles to turkeys. | two female common grackles (quiscalus quiscula) were inoculated intratracheally with 1.6 x 10(8) chick embryo lethal doses50 of a chlamydial organism isolated from turkeys. eight female grackles were kept as uninoculated contacts. six days later, two 12-week-old broad-breasted white turkeys (1 male and 1 female) were placed in the pen with the grackles. chlamydiae were isolated in mice from cloacal swabs taken 14 days postinoculation from the infected grackles. swabs from the contact grackles di ... | 1978 | 678235 |
| [human chlamydia psittaci infection]. | | 1978 | 705936 |
| response of c3h/hej and c3h/hen mice and their peritoneal macrophages to the toxicity of chlamydia psittaci elementary bodies. | intravenous injection of toxic doses of chlamydia psittaci elementary bodies into endotoxin-responsive c3h/hen mice or endotoxin-nonresponsive c3h/hej mice resulted in essentially identical time intervals to death. inoculation of monolayer cultures of thioglycolate-stimulated peritoneal macrophages from the two strains of mice with 250 elementary bodies per macrophage resulted in immediate host cell toxicity, although the c3h/hej macrophages were somewhat less sensitive to elementary body toxici ... | 1978 | 730377 |
| use of enteric vaccines in protection against chlamydial infections of the genital tract and the eye of guinea pigs. | guinea pigs in a test group were fed living guinea pig inclusion conjunctivitis (gpic) organisms classified as chlamydia psittaci in 60% yolk-sac suspensions as enteric vaccines, while animals in a control group received uninfected yolk sac. seven test animals and 14 control animals were challenged 11 or 22 days later with 1,000 50% infectious doses of gpic organisms in either the conjunctiva or the vagina. evidence of protection from mucosal infection in both sites was noted in test animals. cl ... | 1978 | 739153 |
| [experimental infection of guinea pigs with clamydia. 2. fluorescent antibody examinations]. | the direct fluorescent antibody technique was applied to blood smears and organ impression smears from guinea-pigs killed at various times during 13 weeks following nasal infection with a bovine strain of chlamydia. antigen inclusions were demonstrated one hour after infection in blood granulocytes, after 3 hours in most of the organs examined and after 6-12 hours in all the organs. antigen was present throughout the period of observation in blood smears and samples from nasal mucosa, trachea, l ... | 1975 | 776118 |