| complete sequence and genome properties of chinese wheat mosaic virus, a new furovirus from china. | the complete nucleotide sequence of a virus infecting winter wheat in shandong province, china has been determined. this was previously thought to be soil-borne wheat mosaic virus but, while the two viruses are related, they are only 75% (rna1) and 63% (rna2) identical at the nucleotide level, while the amino acid sequences share from 62% (19 kda rna2 product) to 84% (rna1 replicase) identity. the analysis shows that the chinese virus should be considered a new member of the genus furovirus and ... | 1999 | 10355760 |
| sequences of european wheat mosaic virus and oat golden stripe virus and genome analysis of the genus furovirus. | the complete nucleotide sequences of both rnas of oat golden stripe virus (ogsv) and a wheat-infecting furovirus isolate from france, previously thought to be soil-borne wheat mosaic virus (sbwmv), have been determined. both viruses had a similar genomic organisation to sbwmv and chinese wheat mosaic virus, the two other furoviruses previously sequenced but had <70% nucleotides identical to them. the french isolate has been named european wheat mosaic virus (ewmv). phylogenetic analyses supporte ... | 1999 | 10497118 |
| soil-borne viruses affecting cereals: known for long but still a threat. | a number of furo- and bymoviruses, which are naturally transmitted by the ubiquitous soil-borne protozoon polymyxa graminis are of great economic importance for autumn-sown wheat, barley, rye, triticale, and oats. the two barley-affecting bymoviruses, barley yellow mosaic virus and barley mild mosaic virus, are widespread in europe and east asia. production of wheat, rye, and triticale is threatened by three related furoviruses and two bymoviruses. soil-borne wheat mosaic virus mainly occurs in ... | 2009 | 19159654 |
| functional identification of two minor capsid proteins from chinese wheat mosaic virus using its infectious full-length cdna clones. | full-length cdna clones of chinese wheat mosaic virus (cwmv) rna1 and rna2 were produced from single reverse transcription pcr reactions and transcripts were shown to be infectious in both wheat and nicotiana benthamiana. an efficient and reliable agro-infiltration method was then developed for reverse genetic assays in n. benthamiana. inoculation of infectious cdna clones resulted in obvious chlorotic symptoms, and cwmv viral genomic rnas, capsid protein (cp)-related proteins, and typical rod-s ... | 2016 | 27357465 |
| analysis of small rnas derived from chinese wheat mosaic virus. | the virus-derived small interfering rnas (vsirnas) of chinese wheat mosaic virus (cwmv), a member of the genus furovirus, were characterised from wheat plants by deep sequencing. cwmv vsirnas of 21-22 nt in length predominated, suggesting that there might be a conserved mechanism of dcl2 and dcl4 involvement in the biogenesis of vsirnas, as well as a common rna silencing pathway in cwmv-infected wheat plants. the 5'-terminal base of vsirnas was biased towards a/u, suggesting that cwmv vsirnas mi ... | 2014 | 24997977 |
| the cug-initiated larger form coat protein of chinese wheat mosaic virus binds to the cysteine-rich rna silencing suppressor. | some viruses use alternative translation initiation at non-aug codons as a strategy to produce multiple proteins during gene expression. here we show that, using this strategy, chinese wheat mosaic virus (cwmv; furovirus) expresses a larger form of coat protein (n-ext/cp) in infected plants. site-directed mutagenesis and transient expression analysis confirmed that cwmv n-ext/cp is initiated at an upstream in-frame cug codon at nucleotide position 207-209 of rna 2, which adds a 39 amino acid (aa ... | 2013 | 23911633 |
| root-specific role for nicotiana benthamiana rdr6 in the inhibition of chinese wheat mosaic virus accumulation at higher temperatures. | some viruses only infect plants at cool temperatures but the molecular mechanism underlying this low-temperature dependence remains unclear. chinese wheat mosaic virus (cwmv, genus furovirus) was able to infect wheat and nicotiana benthamiana plants at 16 but not at 24°c. when cwmv-infected plants were transferred to 24°c for 2 weeks, the newly emerged leaves and roots became virus free. co-infection with potato virus y rescued cwmv accumulation in n. benthamiana plants after a temperature shift ... | 2013 | 23777430 |
| differential detection of wheat yellow mosaic virus, japanese soil-borne wheat mosaic virus and chinese wheat mosaic virus by reverse transcription loop-mediated isothermal amplification reaction. | a differential detection method for three wheat viruses: wheat yellow mosaic virus (wymv), japanese soil-borne mosaic virus (jsbwmv) and chinese wheat mosaic virus (cwmv) using reverse transcription loop-mediated isothermal amplification (rt-lamp) reaction was developed. all three primer sets, which were designed from the genome sequences of wymv, jsbwmv and cwmv respectively, worked most efficiently at 65 °c and could detect each virus rna within 10 min by fluorescence monitoring using an isoth ... | 2013 | 23523736 |
| identification of the amino acid residues and domains in the cysteine-rich protein of chinese wheat mosaic virus that are important for rna silencing suppression and subcellular localization. | cysteine-rich proteins (crps) encoded by some plant viruses in diverse genera function as rna silencing suppressors. within the n-terminal portion of crps encoded by furoviruses, there are six conserved cysteine residues and a cys-gly-x-x-his motif (cys, cysteine; gly, glycine; his, histidine; x, any amino acid residue) with unknown function. the central domains contain coiled-coil heptad amino acid repeats that usually mediate protein dimerization. here, we present evidence that the conserved c ... | 2013 | 23458485 |
| endoplasmic reticulum export and vesicle formation of the movement protein of chinese wheat mosaic virus are regulated by two transmembrane domains and depend on the secretory pathway. | the 37k protein of chinese wheat mosaic virus (cwmv) belongs to the 30k superfamily of plant virus movement proteins. cwmv 37k trans-complemented the cell-to-cell spread of a movement-defective potato virus x. cwmv 37k fused to enhanced green fluorescent protein localized to plasmodesmata and formed endoplasmic reticulum (er)-derived vesicular and large aggregate structures. cwmv 37k has two putative n-terminal transmembrane domains (tmds). mutations disrupting tmd1 or tmd2 impaired 37k movement ... | 2013 | 23137810 |