| identification of cryptosporidium felis in a cow by morphologic and molecular methods. | apicomplexan cryptosporidium parasites infect a wide range of vertebrate hosts. while some species are limited to a single host group, such as cryptosporidium baileyi, which infects chickens, other species of this genus, such as c. parvum, infect a wide range of mammalian species from mice to humans. during an investigation of cryptosporidium infection in cattle on a farm in northern poland, we identified an infection caused by c. felis, in addition to known infections with c. muris and c. parvu ... | 1999 | 10103236 |
| presence of heterogeneous copies of the small subunit rrna gene in cryptosporidium parvum human and marsupial genotypes and cryptosporidium felis. | | 1999 | 10519242 |
| phylogenetic relationships among isolates of cryptosporidium: evidence for several new species. | isolates of cryptosporidium were characterized using nucleotide sequence analysis of the 18s rrna and dihydrofolate reductase genes and also random-amplified polymorphic dna analysis. phylogenetic analysis confirmed the validity of the species of cryptosporidium examined in this study such as cryptospordium muris and cryptosporidium baileyi, and also reinforced evidence from numerous researchers worldwide suggesting that cryptosporidium parvum is not a single uniform species. the data obtained p ... | 1999 | 10647047 |
| morphologic, host specificity, and molecular characterization of a hungarian cryptosporidium meleagridis isolate. | this study was undertaken in order to characterize cryptosporidium meleagridis isolated from a turkey in hungary and to compare the morphologies, host specificities, organ locations, and small-subunit rna (ssu rrna) gene sequences of this organism and other cryptosporidium species. the phenotypic differences between c. meleagridis and cryptosporidium parvum hungarian calf isolate (zoonotic genotype) oocysts were small, although they were statistically significant. oocysts of c. meleagridis were ... | 2000 | 10653744 |
| molecular characterization of cryptosporidium isolates obtained from human immunodeficiency virus-infected individuals living in switzerland, kenya, and the united states. | a total of 22 cryptosporidium isolates from human immunodeficiency virus-infected patients from kenya, switzerland, and the united states were examined at three genetic loci: the 18s ribosomal dna, hsp-70, and acetyl coenzyme a synthetase genes. four distinct cryptosporidium genotypes were identified: (i) the cryptosporidium parvum "human" genotype, (ii) the c. parvum "cattle" genotype, (iii) cryptosporidium felis, and (iv) cryptosporidium meleagridis. this is the first report of c. meleagridis ... | 2000 | 10699017 |
| morphologic, host specificity, and genetic characterization of a european cryptosporidium andersoni isolate. | this study was undertaken in order to characterize a cryptosporidium muris-like parasite isolated from cattle in hungary and to compare this strain with other cryptosporidium species. to date, the large-type oocysts isolated from cattle were considered as c. muris described from several mammals. the size, form, and structure of the oocysts of the hungarian strain were identical with those described by others from cattle. an apparent difference between the morphometric data of c. muris-like paras ... | 2000 | 11191899 |
| unusual cryptosporidium species recovered from human faeces: first description of cryptosporidium felis and cryptosporidium 'dog type' from patients in england. | dna was extracted from faecal samples collected from 1680 patients in which cryptosporidium oocysts were recognised by light microscopy. dna from faeces from five of these patients failed to amplify by pcr three gene fragments--the cryptosporidium oocyst wall protein (cowp) gene, the thrombospondin-related adhesive protein of cryptosporidium-1 (trap-c1) gene and the thrombospondin-related adhesive protein of cryptosporidium-2 (trap-c2) gene--with primers designed from c. parvum sequences. howeve ... | 2001 | 11232777 |
| human infection with cryptosporidium felis: case report and literature review. | an infection with cryptosporidium felis in an hiv-positive man from italy was successfully treated with paromomycin, despite the patient's having a cd4+ cell count of 31/ml. fourteen cases of human infection with c. felis have been described, all in the past 3 years, emphasizing the public health importance of cryptosporidium parasites other than c. parvum. | 2002 | 11749756 |
| pcr-restriction fragment length polymorphism analysis of a diagnostic 452-base-pair dna fragment discriminates between cryptosporidium parvum and c. meleagridis and between c. parvum isolates of human and animal origin. | genomic dnas from human cryptosporidium isolates previously typed by analysis of the 18s ribosomal dna locus (cryptosporidium parvum bovine genotype, c. parvum human genotype, cryptosporidium meleagridis, and cryptosporidium felis) were used to amplify the diagnostic fragment described by laxer et al. (m. a. laxer, b. k. timblin, and r. j. patel, am. j. trop. med. hyg., 45:688-694, 1991). the obtained 452-bp amplified fragments were sequenced and aligned with the homologous cryptosporidium wrair ... | 2002 | 11916736 |
| molecular epidemiological analysis of cryptosporidium isolates from humans and animals by using a heteroduplex mobility assay and nucleic acid sequencing based on a small double-stranded rna element. | two extrachromosomal double-stranded rna (dsrna) elements occur in cryptosporidium parvum. a heteroduplex mobility assay (hma) was developed for the rapid characterization of sequence diversity in a 173-bp fragment of the small dsrna element of cryptosporidium with either a natural sequence from cryptosporidium meleagridis or a synthetic sequence as reference dna. the 173-bp fragment was generated from 265 samples of whole feces (242 from humans and 18 from livestock with c. parvum genotype 1 or ... | 2003 | 12624019 |
| a novel multiplex polymerase chain reaction approach for detection of four human infective cryptosporidium isolates: cryptosporidium parvum, types h and c, cryptosporidium canis, and cryptosporidium felis in fecal and soil samples. | a nested multiplex polymerase chain reaction (pcr) approach was adopted for the simultaneous detection of 4 human infective genotypes of the protozoan parasite cryptosporidium. specific pcr primers were designed for the heat shock protein 70 gene of 2 genotypes of cryptosporidium parvum (human and bovine types), cryptosporidium canis, and cryptosporidium felis. these 4 genotypes have all been found in human fecal samples. the primers amplified dna fragments of specific sizes, each representing a ... | 2003 | 12735348 |
| cryptosporidium felis and c. meleagridis in persons with hiv, portugal. | | 2004 | 15672531 |
| detection of cryptosporidium and identification to the species level by nested pcr and restriction fragment length polymorphism. | cryptosporidiosis is an emerging protozoan disease associated with large waterborne outbreaks. diagnosis relies on microscopic examination of stools, but this method cannot identify the infecting species of cryptosporidium. we have developed a test based on nested pcr and restriction fragment length polymorphism (rflp) that offers simple identification of cryptosporidium hominis, cryptosporidium parvum, and most other human infective species in stool samples. purified c. parvum oocysts were used ... | 2005 | 15750054 |
| new genotypes and factors associated with cryptosporidium detection in mussels (mytilus spp.) along the california coast. | a 3 year study was conducted to evaluate mussels as bioindicators of faecal contamination in coastal ecosystems of california. haemolymph samples from 4680 mussels (mytilus spp.) were tested for cryptosporidium genotypes using pcr amplification and dna sequence analysis. our hypotheses were that mussels collected from sites near livestock runoff or human sewage outflow would be more likely to contain the faecal pathogen cryptosporidium than mussels collected distant to these sites, and that the ... | 2005 | 15993883 |
| detection of cryptosporidium felis and giardia duodenalis assemblage f in a cat colony. | eighteen cats, 3-6 months of age, bred and housed in a closed colony, were transferred from that colony and placed in separate stainless steel cages in a building designed for housing animals. at daily intervals, feces were collected from the litter pans in each cage, pans and cages were cleaned, and fresh food and water were provided. beginning 4 weeks after the transfer, oocysts of cryptosporidium were detected in the feces of two cats by brightfield microscopy. for the following 21 days, with ... | 2006 | 16621289 |
| characterisation of small double stranded rna molecule in cryptosporidium hominis, cryptosporidium felis and cryptosporidium meleagridis. | coding regions of double stranded rna molecules from 3 human faecal samples containing cryptosporidium hominis, c. felis and c. meleagridis were characterised by sequencing and compared with that previously obtained for c. parvum. sequences outside the coding regions were also obtained. overall similarities of between 86% and 92% and between 86% and 93% were observed in the nucleotide and amino acid sequences respectively between these species. these larger sequences will allow further molecular ... | 2006 | 16890010 |
| [worldwide human zoonotic cryptosporidiosis caused by cryptosporidium felis]. | cryptosporidium is an important enteric pathogen worldwide distributed causing diarrhoeal illness in humans and animals. identifying cryptosporidium species using conventional criteria, such as oocyst morphology, is inadequate. the advent of molecular techniques has conducted to characterize different species and genotypes of cryptosporidium infecting humans. the vast majority of human cases of cryptosporidiosis in the world are caused by both species, cryptosporidium hominis and cryptosporidium ... | 2007 | 17432054 |
| molecular identification of cryptosporidium spp. from fecal samples of felines, canines and bovines in the state of são paulo, brazil. | the objective of this study was to obtain information of epidemiological nature through genotypic characterization of cryptosporidium isolates from dogs, cats and bovines from the state of são paulo, brazil. the extraction of dna from oocysts was carried out and polymerase chain reaction was accomplished using specific primers to 18s rrna gene. the amplicons were directed sequenced. seven cat samples, nine dog samples and nine bovine samples were analysed. from the seven cat samples the genotypi ... | 2007 | 17996373 |
| multilocus sequence typing and genetic structure of cryptosporidium hominis from children in kolkata, india. | endemicity of cryptosporidiosis in india has been documented with little genetic characterization of the parasites. fifty cryptosporidium-positive specimens collected between 2001 and 2004 from pediatric patients in kolkata, india were analyzed for parasite genetic structure using multilocus sequence typing (mlst). genotype analyses showed the presence of cryptosporidium hominis, cryptosporidium meleagridis and cryptosporidium felis in 49, 2 and 1 patients, respectively (two patients had mixed i ... | 2007 | 17010677 |
| genetic analysis of cryptosporidium from 2414 humans with diarrhoea in england between 1985 and 2000. | the characterization of cryptosporidium using dna extracted from whole faecal samples collected from 2414 humans with diarrhoea in england between 1985 and 2000 where cryptosporidial oocysts were detected using conventional methods is described. characterization was achieved by pcr/rflp and dna sequencing of fragments of the cryptosporidium oocyst wall protein and the 18s rdna genes. cryptosporidium parvum was detected in 56.1 % of cases, cryptosporidium hominis in 41.7 % and a mixture of c. par ... | 2006 | 16687587 |
| molecular epidemiology of cryptosporidium in humans and cattle in the netherlands. | the protozoan parasite cryptosporidium is found world-wide and can cause disease in both humans and animals. to study the zoonotic potential of cryptosporidium in the netherlands we isolated this parasite from the faeces of infected humans and cattle and genotyped those isolates for several different markers. the overall genotyping results showed: for humans isolates, 70% cryptosporidium hominis, 19% cryptosporidium parvum, 10% a combination of c. hominis and c. parvum, and 1% cryptosporidium fe ... | 2008 | 18054936 |
| morphological and molecular characterisation of a mixed cryptosporidium muris/cryptosporidium felis infection in a cat. | to date cryptosporidium muris has been identified by microscopy and genotyping in cats in two studies. we report morphological and genetic evidence of a mixed c. muris and c. felis infection in a cat and provide the first histological, immunohistochemical, in situ hybridisation and genetic confirmation of a c. muris infection in the stomach of a cat. the cat suffered persistent diarrhoea after the initial consultation, which remained unresolved, despite several medical interventions. further stu ... | 2010 | 21044819 |
| comparison of the diagnostic sensitivity and specificity of seven cryptosporidium assays used in the united kingdom. | to compare the diagnostic sensitivity and specificity of seven cryptosporidium diagnostic assays used in the uk, results from 259 stool samples from patients with acute gastrointestinal symptoms were compared against a nominated gold standard (real-time pcr and oocyst detection). of the 152 'true positives', 80 were c. hominis, 68 c. parvum, two c. felis, one c. ubiquitum and one c. meleagridis. cryptosporidium spp. diagnostic sensitivities of three cryptosporidium and giardia combination copro- ... | 2011 | 21757501 |
| genotyping of cryptosporidium spp. in environmental water in turkey. | this research was undertaken to study the molecular detection and characterization of cryptosporidium spp. in environmental water sources at samsun and giresun provinces of the black sea in turkey. two-hundred forty and one-hundred eighty environmental samples were collected from a total of twenty and twenty-five sampling sites of giresun and samsun provinces. one hundred twenty untreated drinking water samples were also detected for cryptosporidium spp. in both investigated areas. 101 (%42), 92 ... | 2016 | 27787219 |
| molecular detection and characterization of cryptosporidium spp. among breeding cattery cats in japan. | cryptosporidium spp. are pathogenic protozoan that can cause gastrointestinal illness in mammalian hosts. as a result of the close contact between humans and cats, there is concern regarding the potential zoonotic transmission of cryptosporidium spp. from infected cats; however, few data have been reported regarding the prevalence of this pathogen among cats. here, we report the prevalence of cryptosporidium spp. among breeding cattery cats in japan. a total of 286 fresh fecal samples were colle ... | 2016 | 26960959 |
| possible zoonotic transmission of cryptosporidium felis in a household. | in humans, the risk of contracting cryptosporidiosis caused by cryptosporidium felis is considered to be relatively low, and most of the confirmed cases have been observed in immunocompromised patients. both anthroponotic and zoonotic transmission routes have been suggested. here, we report a case of suspected zoonotic transmission of c. felis from a cat to a human. the cat developed diarrhea several months before such symptoms were displayed by its owner, a 37-year-old immunocompetent woman. th ... | 2015 | 26446304 |
| molecular characterization of cryptosporidium spp., giardia duodenalis, and enterocytozoon bieneusi in captive wildlife at zhengzhou zoo, china. | cryptosporidium spp., giardia duodenalis, and enterocytozoon bieneusi are common gastrointestinal protists in humans and animals. two hundred and three fecal specimens from 80 wildlife species were collected in zhengzhou zoo and their genomic dna extracted. three intestinal pathogens were characterized with a dna sequence analysis of different loci. cryptosporidium felis, c. baileyi, and avian genotype iii were identified in three specimens (1.5%), the manul, red-crowned crane, and cockatiel, re ... | 2015 | 26384582 |
| unexpected finding of feline-specific giardia duodenalis assemblage f and cryptosporidium felis in asymptomatic adult cattle in northern spain. | giardia duodenalis and cryptosporidium spp. are common enteric protozoan parasites in production animals, including cattle. typically, both the clinical outcome of these infections and the distribution of g. duodenalis assemblages and cryptosporidium species are age-dependent, with the occurrence of diarrhoeal disease being mainly associated with young animals and sub-clinical, low intensity infections being predominantly found in adult animals. to investigate the prevalence and genetic diversit ... | 2015 | 25771933 |
| enteric protozoa of cats and their zoonotic potential-a field study from austria. | domestic cats can be infected with a variety of enteric protozoa. genotyping of protozoan species, especially giardia as the most common, can improve assessment of their relevance as zoonotic agents. for an overview on the occurrence of feline enteric protozoa, 298 faecal samples of cats from private households, catteries and animal shelters in austria were collected. all samples were examined by flotation and using a rapid test for giardia (fastest). for the detection of tritrichomonas blagburn ... | 2015 | 25762189 |
| a retrospective molecular study of select intestinal protozoa in healthy pet cats from italy. | the feline gut can harbour a number of protozoan parasites. recent genetic studies have highlighted new epidemiological findings about species of cryptosporidium, assemblages of giardia duodenalis and toxoplasma gondii. furthermore, epidemiological studies suggest the occurrence of tritrichomonas foetus in cats is on the increase worldwide. the prevalence of selected intestinal protozoa was determined by pcr using dna previously extracted from the faeces of 146 privately owned healthy cats from ... | 2015 | 24793744 |
| cryptosporidium felis in faeces from cats in the uk. | | 2014 | 24789855 |
| two cases of zoonotic cryptosporidiosis in spain by the unusual species cryptosporidium ubiquitum and cryptosporidium felis. | two cases of infection by zoonotic transmission of unusual species of cryptosporidium were detected in 2010-2011 in spain (león and zaragoza). | 2012 | 22728073 |
| cryptosporidium felis infection, spain. | | 2006 | 17073113 |
| prevalence of and risk factors associated with shedding of cryptosporidium felis in domestic cats of mississippi and alabama. | the prevalence of and risk factors associated with the shedding of cryptosporidial oocysts were determined for domestic cats in northeastern mississippi and northwestern alabama. cryptosporidial oocysts were found in a single fecal samples from 30 of 250 cats using a centrifugal concentration technique followed by an immunofluorescent assay. the odds of a cat shedding oocysts were increased when another cat shedding oocysts was present in the household. logistic regression analysis showed only c ... | 2009 | 19117680 |
| long-term cryptosporidium typing reveals the aetiology and species-specific epidemiology of human cryptosporidiosis in england and wales, 2000 to 2003. | to improve understanding of the aetiology and epidemiology of human cryptosporidiosis, over 8,000 cryptosporidium isolates were submitted for typing to the species level over a four year period. the majority were either cryptosporidium parvum (45.9%) or cryptosporidium hominis (49.2%). dual infection occurred in 40 (0.5%) cases and six other known cryptosporidium species or genotypes were found in 67 (0.9%) cases. these were cryptosporidium meleagridis, cryptosporidium felis, cryptosporidium can ... | 2009 | 19161717 |